General information | |||
TC008294 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 8065718..8071852 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC008294 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08156 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
mandible shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs potentially size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen cuticle posterior structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC008294 RA
MQAASNGCV QNGAPSSSN GASENGHED TSNGPAVQL TQTSQDIVR LIGQYLKNE GLTRTADSL MAESGCRLD HPAAAKFRH HVMDGDWTK ADHDLQELQ NLIASSNSL LEMKFLLLE QKYLEYLED GRVLDALHV LRNELTPLQ HNTSRVHQL SSYMMCSNT QELHERTNW DGKGLKSRT ILMDKLQTY LPPSVMLPP SRLHTLLNQ ALELQTLHC SHHNTAQQI TLDNASLLV DHCCARDTF PTHTIQILN DHCDEVWFC QFSPDGTKL ATGSKDTNV IIWDVHPET CTLSLKKIL EGHNYGASY ISWSPDSVH LIACGPEES PEVWLWNIE TEKFLKVSQ SPEDALACC AWHKDGTKF VVGGIRGHF YQCDMEGTV IDSWEGVRV NGLWCRKDG KTVIASDTH HRIRGYIFE ELSDHQILQ EDHAIMSFT VDKNDKLAL LNVATQGVH LWDLNDKCL VRRYQGVTQ GHFTIHSTF GGANQDFIA SGSEDNQVY IWHIRNEQP IAVLTGHTR TVNCVAWNP VYPQMLVSV SDDCTVRVW GPKDKVPKP TVSLSSSDS SNNSTWNEM IS
- >TC008294 RA
atgcaagcg gccagcaac ggatgcgtg caaaatggg gccccctcg tccagcaac ggggcttcg gagaatgga cacgaggac acttccaat gggcccgcc gtgcaattg acacagact agtcaggac attgtcagg ctcatcggc caatacctc aaaaatgaa gggctaaca cgaacggcc gactcttta atggccgaa tccgggtgc cgtttggac caccccgca gctgccaaa tttcgccac cacgtaatg gacggtgac tggacaaag gcagatcac gatctgcaa gaattacag aatttaatt gcatcttcc aatagctta ttggaaatg aagttttta ttactcgaa cagaaatat ctggaatat ttagaagac gggagagtt ttggatgct ctccacgtg ttgcgaaat gaattaaca cctctacaa cacaatact tcaagagtg catcaactc tcaagctac atgatgtgt tcaaatacg caagaattg cacgaacgt actaattgg gatgggaag ggtttaaag tcccgaaca attttgatg gacaaactc caaacctat ctgcccccg agtgttatg ttaccgcct agccgacta catactttg ctcaatcaa gcgcttgag ttacaaact ttacattgt agccatcat aacacggca cagcagatc accctggac aacgcctcg ctcttagtt gaccactgc tgtgccaga gacacgttt cctactcat actatacaa atcttaaac gatcactgt gatgaggtc tggttctgc cagttttcg cccgatggg acgaagctg gcgaccgga tccaaagat accaatgtg attatttgg gacgtccac ccggagact tgcacgctc tccctgaaa aaaatcctc gaaggacac aattacgga gcttcgtac atatcgtgg agtcccgat tcggttcat ttaattgcg tgcggtccg gaggaaagt cccgaggtt tggttgtgg aatatcgaa actgaaaaa ttcctcaaa gtctcgcaa tctcccgaa gatgctttg gcgtgctgt gcatggcat aaagacggt acaaaattt gtggtgggt gggattaga ggtcatttt tatcagtgt gatatggaa ggtactgtc atagattcg tgggaggga gtccgtgtg aacggtttg tggtgtcgg aaagacggg aaaacggtc attgcgtcg gatacacat caccggatc aggggctac atctttgag gagctttca gaccatcaa atattgcaa gaagatcac gctatcatg tcgttcacc gttgacaaa aacgacaaa cttgcgctt ttaaacgtt gccacacaa ggtgttcat ctatgggac ttaaacgat aaatgctta gttagacga taccaagga gttactcag ggtcatttt acaatacat tcaactttt ggaggagct aaccaggac tttatcgcc agtgggagt gaagacaat caggtctac atctggcat attcgaaac gagcaaccc attgctgtt ttaactggc catacaaga acagtaaac tgtgttgcc tggaatcct gtttatcca caaatgctt gtttcagtt tctgacgac tgtacagtt agggtatgg ggaccaaaa gataaggtg cctaaacca acagtgtct ttatctagt agcgattca agtaataac agcacatgg aacgaaatg atttcatag
- >TC008294 RA
gttatatta tataaaaaa cggaaaaaa tgtttttgc aaacttgga aagctattt ttggaacaa aggtaattg acctagttc gctggttat gcaaccagg aattcacac ttgtggttg gttatttta ttgcttgaa caacggtgt tttaaggtt ggtaacggt aagttcggg tggttgttg ttgtctctt tctggtcgt caaatttcc tttggttct tcttcacgt ggcaatatt ttcaaatca aatgttaat gtggaacaa agatgagga tataacgtg tatttagtg gaggattgg taaataaag agcacctca gcggatgtg taccgtctg acatttcag ctgttcagc ccgtttgtt gttgttagt ggaatatca actcccagc gtttattat atggactgt tgaggtcaa gccaccagc tgttttctg ggttgttgg aatttgaac catttgtcc attgaacaa acagttggt gagcgttgg ttcagctgt aattcagct gatgtttgt tgtggggtg gtgcagttt ctaggattg tcaatttcc tcaaaaaat tcacgtgat tttccactc aaagaatga agagtggtt gaaaacaac atctccatg gtcaacaat cgaaatctg cgtgattac cagtgtgac gtaataaat ggatctgtg aaaacaaag agcccgttt taattaatg ttaatcgcg tctaaagta aacgtgatc gtggaaagt gaggaggaa aagtgcaaa aataagtct ggcctctaa tagtgattc atccgggga cgacggtgg acagccgcc tcgtaaaaa aacccgaag agacaatag tattgcgat tgacaccac cataatgca agcggccag caacggatg cgtgcaaaa tggggcccc ctcgtccag caacggggc ttcggagaa tggacacga ggacacttc caatgggcc cgccgtgca attgacaca gactagtca ggacattgt caggctcat cggccaata cctcaaaaa tgaagggct aacacgaac ggccgactc tttaatggc cgaatccgg gtgccgttt ggaccaccc cgcagctgc caaatttcg ccaccacgt aatggacgg tgactggac aaaggcaga tcacgatct gcaagaatt acagaattt aattgcatc ttccaatag cttattgga aatgaagtt tttattact cgaacagaa atatctgga atatttaga agacgggag agttttgga tgctctcca cgtgttgcg aaatgaatt aacacctct acaacacaa tacttcaag agtgcatca actctcaag ctacatgat gtgttcaaa tacgcaaga attgcacga acgtactaa ttgggatgg gaagggttt aaagtcccg aacaatttt gatggacaa actccaaac ctatctgcc cccgagtgt tatgttacc gcctagccg actacatac tttgctcaa tcaagcgct tgagttaca aactttaca ttgtagcca tcataacac ggcacagca gatcaccct ggacaacgc ctcgctctt agttgacca ctgctgtgc cagagacac gtttcctac tcatactat acaaatctt aaacgatca ctgtgatga ggtctggtt ctgccagtt ttcgcccga tgggacgaa gctggcgac cggatccaa agataccaa tgtgattat ttgggacgt ccacccgga gacttgcac gctctccct gaaaaaaat cctcgaagg acacaatta cggagcttc gtacatatc gtggagtcc cgattcggt tcatttaat tgcgtgcgg tccggagga aagtcccga ggtttggtt gtggaatat cgaaactga aaaattcct caaagtctc gcaatctcc cgaagatgc tttggcgtg ctgtgcatg gcataaaga cggtacaaa atttgtggt gggtgggat tagaggtca tttttatca gtgtgatat ggaaggtac tgtcataga ttcgtggga gggagtccg tgtgaacgg tttgtggtg tcggaaaga cgggaaaac ggtcattgc gtcggatac acatcaccg gatcagggg ctacatctt tgaggagct ttcagacca tcaaatatt gcaagaaga tcacgctat catgtcgtt caccgttga caaaaacga caaacttgc gcttttaaa cgttgccac acaaggtgt tcatctatg ggacttaaa cgataaatg cttagttag acgatacca aggagttac tcagggtca ttttacaat acattcaac ttttggagg agctaacca ggactttat cgccagtgg gagtgaaga caatcaggt ctacatctg gcatattcg aaacgagca acccattgc tgttttaac tggccatac aagaacagt aaactgtgt tgcctggaa tcctgttta tccacaaat gcttgtttc agtttctga cgactgtac agttagggt atggggacc aaaagataa ggtgcctaa accaacagt gtctttatc tagtagcga ttcaagtaa taacagcac atggaacga aatgatttc atagtgact acttacaaa gactgagcg agaaaacat catctctta agtttattt attggctat acagcgata aggatttaa ggtaggagt caaaattgt atctgtttg gaatagtgc tccaatgag tgtaattaa gattaaagc ttttataaa cgaggaggt ttatcaata atgttatta catagtgat attaacaca gacaattat ttaaaatag agtgtaagt aattaaaag gaatgtatc ttatctcca aataaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
