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Gene information
General information
TC008294 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8065718..8071852
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008294
    iBeetle screen iB_08156

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    mandible shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs potentially size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen cuticle posterior structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008294 RA
      MQAASNGCV QNGAPSSSN GASENGHED TSNGPAVQL 
      TQTSQDIVR LIGQYLKNE GLTRTADSL MAESGCRLD 
      HPAAAKFRH HVMDGDWTK ADHDLQELQ NLIASSNSL 
      LEMKFLLLE QKYLEYLED GRVLDALHV LRNELTPLQ 
      HNTSRVHQL SSYMMCSNT QELHERTNW DGKGLKSRT 
      ILMDKLQTY LPPSVMLPP SRLHTLLNQ ALELQTLHC 
      SHHNTAQQI TLDNASLLV DHCCARDTF PTHTIQILN 
      DHCDEVWFC QFSPDGTKL ATGSKDTNV IIWDVHPET 
      CTLSLKKIL EGHNYGASY ISWSPDSVH LIACGPEES 
      PEVWLWNIE TEKFLKVSQ SPEDALACC AWHKDGTKF 
      VVGGIRGHF YQCDMEGTV IDSWEGVRV NGLWCRKDG 
      KTVIASDTH HRIRGYIFE ELSDHQILQ EDHAIMSFT 
      VDKNDKLAL LNVATQGVH LWDLNDKCL VRRYQGVTQ 
      GHFTIHSTF GGANQDFIA SGSEDNQVY IWHIRNEQP 
      IAVLTGHTR TVNCVAWNP VYPQMLVSV SDDCTVRVW 
      GPKDKVPKP TVSLSSSDS SNNSTWNEM IS
    CDS sequence
    • >TC008294 RA
      atgcaagcg gccagcaac ggatgcgtg caaaatggg 
      gccccctcg tccagcaac ggggcttcg gagaatgga 
      cacgaggac acttccaat gggcccgcc gtgcaattg 
      acacagact agtcaggac attgtcagg ctcatcggc 
      caatacctc aaaaatgaa gggctaaca cgaacggcc 
      gactcttta atggccgaa tccgggtgc cgtttggac 
      caccccgca gctgccaaa tttcgccac cacgtaatg 
      gacggtgac tggacaaag gcagatcac gatctgcaa 
      gaattacag aatttaatt gcatcttcc aatagctta 
      ttggaaatg aagttttta ttactcgaa cagaaatat 
      ctggaatat ttagaagac gggagagtt ttggatgct 
      ctccacgtg ttgcgaaat gaattaaca cctctacaa 
      cacaatact tcaagagtg catcaactc tcaagctac 
      atgatgtgt tcaaatacg caagaattg cacgaacgt 
      actaattgg gatgggaag ggtttaaag tcccgaaca 
      attttgatg gacaaactc caaacctat ctgcccccg 
      agtgttatg ttaccgcct agccgacta catactttg 
      ctcaatcaa gcgcttgag ttacaaact ttacattgt 
      agccatcat aacacggca cagcagatc accctggac 
      aacgcctcg ctcttagtt gaccactgc tgtgccaga 
      gacacgttt cctactcat actatacaa atcttaaac 
      gatcactgt gatgaggtc tggttctgc cagttttcg 
      cccgatggg acgaagctg gcgaccgga tccaaagat 
      accaatgtg attatttgg gacgtccac ccggagact 
      tgcacgctc tccctgaaa aaaatcctc gaaggacac 
      aattacgga gcttcgtac atatcgtgg agtcccgat 
      tcggttcat ttaattgcg tgcggtccg gaggaaagt 
      cccgaggtt tggttgtgg aatatcgaa actgaaaaa 
      ttcctcaaa gtctcgcaa tctcccgaa gatgctttg 
      gcgtgctgt gcatggcat aaagacggt acaaaattt 
      gtggtgggt gggattaga ggtcatttt tatcagtgt 
      gatatggaa ggtactgtc atagattcg tgggaggga 
      gtccgtgtg aacggtttg tggtgtcgg aaagacggg 
      aaaacggtc attgcgtcg gatacacat caccggatc 
      aggggctac atctttgag gagctttca gaccatcaa 
      atattgcaa gaagatcac gctatcatg tcgttcacc 
      gttgacaaa aacgacaaa cttgcgctt ttaaacgtt 
      gccacacaa ggtgttcat ctatgggac ttaaacgat 
      aaatgctta gttagacga taccaagga gttactcag 
      ggtcatttt acaatacat tcaactttt ggaggagct 
      aaccaggac tttatcgcc agtgggagt gaagacaat 
      caggtctac atctggcat attcgaaac gagcaaccc 
      attgctgtt ttaactggc catacaaga acagtaaac 
      tgtgttgcc tggaatcct gtttatcca caaatgctt 
      gtttcagtt tctgacgac tgtacagtt agggtatgg 
      ggaccaaaa gataaggtg cctaaacca acagtgtct 
      ttatctagt agcgattca agtaataac agcacatgg 
      aacgaaatg atttcatag
    mRNA sequence
    • >TC008294 RA
      gttatatta tataaaaaa cggaaaaaa tgtttttgc 
      aaacttgga aagctattt ttggaacaa aggtaattg 
      acctagttc gctggttat gcaaccagg aattcacac 
      ttgtggttg gttatttta ttgcttgaa caacggtgt 
      tttaaggtt ggtaacggt aagttcggg tggttgttg 
      ttgtctctt tctggtcgt caaatttcc tttggttct 
      tcttcacgt ggcaatatt ttcaaatca aatgttaat 
      gtggaacaa agatgagga tataacgtg tatttagtg 
      gaggattgg taaataaag agcacctca gcggatgtg 
      taccgtctg acatttcag ctgttcagc ccgtttgtt 
      gttgttagt ggaatatca actcccagc gtttattat 
      atggactgt tgaggtcaa gccaccagc tgttttctg 
      ggttgttgg aatttgaac catttgtcc attgaacaa 
      acagttggt gagcgttgg ttcagctgt aattcagct 
      gatgtttgt tgtggggtg gtgcagttt ctaggattg 
      tcaatttcc tcaaaaaat tcacgtgat tttccactc 
      aaagaatga agagtggtt gaaaacaac atctccatg 
      gtcaacaat cgaaatctg cgtgattac cagtgtgac 
      gtaataaat ggatctgtg aaaacaaag agcccgttt 
      taattaatg ttaatcgcg tctaaagta aacgtgatc 
      gtggaaagt gaggaggaa aagtgcaaa aataagtct 
      ggcctctaa tagtgattc atccgggga cgacggtgg 
      acagccgcc tcgtaaaaa aacccgaag agacaatag 
      tattgcgat tgacaccac cataatgca agcggccag 
      caacggatg cgtgcaaaa tggggcccc ctcgtccag 
      caacggggc ttcggagaa tggacacga ggacacttc 
      caatgggcc cgccgtgca attgacaca gactagtca 
      ggacattgt caggctcat cggccaata cctcaaaaa 
      tgaagggct aacacgaac ggccgactc tttaatggc 
      cgaatccgg gtgccgttt ggaccaccc cgcagctgc 
      caaatttcg ccaccacgt aatggacgg tgactggac 
      aaaggcaga tcacgatct gcaagaatt acagaattt 
      aattgcatc ttccaatag cttattgga aatgaagtt 
      tttattact cgaacagaa atatctgga atatttaga 
      agacgggag agttttgga tgctctcca cgtgttgcg 
      aaatgaatt aacacctct acaacacaa tacttcaag 
      agtgcatca actctcaag ctacatgat gtgttcaaa 
      tacgcaaga attgcacga acgtactaa ttgggatgg 
      gaagggttt aaagtcccg aacaatttt gatggacaa 
      actccaaac ctatctgcc cccgagtgt tatgttacc 
      gcctagccg actacatac tttgctcaa tcaagcgct 
      tgagttaca aactttaca ttgtagcca tcataacac 
      ggcacagca gatcaccct ggacaacgc ctcgctctt 
      agttgacca ctgctgtgc cagagacac gtttcctac 
      tcatactat acaaatctt aaacgatca ctgtgatga 
      ggtctggtt ctgccagtt ttcgcccga tgggacgaa 
      gctggcgac cggatccaa agataccaa tgtgattat 
      ttgggacgt ccacccgga gacttgcac gctctccct 
      gaaaaaaat cctcgaagg acacaatta cggagcttc 
      gtacatatc gtggagtcc cgattcggt tcatttaat 
      tgcgtgcgg tccggagga aagtcccga ggtttggtt 
      gtggaatat cgaaactga aaaattcct caaagtctc 
      gcaatctcc cgaagatgc tttggcgtg ctgtgcatg 
      gcataaaga cggtacaaa atttgtggt gggtgggat 
      tagaggtca tttttatca gtgtgatat ggaaggtac 
      tgtcataga ttcgtggga gggagtccg tgtgaacgg 
      tttgtggtg tcggaaaga cgggaaaac ggtcattgc 
      gtcggatac acatcaccg gatcagggg ctacatctt 
      tgaggagct ttcagacca tcaaatatt gcaagaaga 
      tcacgctat catgtcgtt caccgttga caaaaacga 
      caaacttgc gcttttaaa cgttgccac acaaggtgt 
      tcatctatg ggacttaaa cgataaatg cttagttag 
      acgatacca aggagttac tcagggtca ttttacaat 
      acattcaac ttttggagg agctaacca ggactttat 
      cgccagtgg gagtgaaga caatcaggt ctacatctg 
      gcatattcg aaacgagca acccattgc tgttttaac 
      tggccatac aagaacagt aaactgtgt tgcctggaa 
      tcctgttta tccacaaat gcttgtttc agtttctga 
      cgactgtac agttagggt atggggacc aaaagataa 
      ggtgcctaa accaacagt gtctttatc tagtagcga 
      ttcaagtaa taacagcac atggaacga aatgatttc 
      atagtgact acttacaaa gactgagcg agaaaacat 
      catctctta agtttattt attggctat acagcgata 
      aggatttaa ggtaggagt caaaattgt atctgtttg 
      gaatagtgc tccaatgag tgtaattaa gattaaagc 
      ttttataaa cgaggaggt ttatcaata atgttatta 
      catagtgat attaacaca gacaattat ttaaaatag 
      agtgtaagt aattaaaag gaatgtatc ttatctcca 
      aataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY