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Gene information
General information
TC007177 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 8027729..8029256
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007177
    iBeetle screen iB_08155

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007177 RA
      MSKHPYNYG ATASTDVGF IGKRSPEFN NLCDGVVTN 
      IYTINSSLK TLDNALKTI GTRKDNQGL RSSIHVTQL 
      STNQIASVT SKDIHKLKQ LVSKSEKQQ QLQVEKLEE 
      NFKEAITRY YSLQKDLAN KQKSHLLVS VSIENDYTP 
      EEDTEQQRQ AQLAREMAF EQDMLMERE ARIKQIEAD 
      VLDVNEIMR ELGSLVHAQ AETIDTIEN SIDHATGNV 
      EEATEQLIT AASYQNKYR RKLLFMVLI GLAIVAIII 
      IILVVQFKK
    CDS sequence
    • >TC007177 RA
      atgagtaaa catccctac aattacggt gctactgcg 
      tccactgac gtaggtttc atcggtaag cgctccccc 
      gaattcaat aatttatgc gatggcgtc gtaacaaac 
      atttacaca atcaactca agtctcaag accttagac 
      aacgcttta aaaacaatc ggaaccaga aaagacaat 
      cagggatta agaagttca atacacgtc actcaattg 
      agcacaaac cagatcgca tcagtcaca agcaaagac 
      attcacaag ctcaagcaa cttgtatcc aaaagcgag 
      aaacagcag cagttgcaa gtagaaaag ttagaagag 
      aattttaaa gaagcgatc acgcgctac tattcctta 
      caaaaggac ttggccaac aagcagaaa tcacattta 
      ttggtttct gtaagcata gaaaatgac tacacgccg 
      gaagaagac acagaacag caacggcaa gctcagctt 
      gcacgcgaa atggcgttt gagcaagat atgctcatg 
      gagagagaa gctaggatt aaacaaatt gaagccgat 
      gtacttgat gttaacgaa atcatgcgc gaattgggg 
      tccttagtg cacgcccaa gcggaaaca atcgatact 
      attgaaaat agcatagac catgcaact ggcaatgtg 
      gaagaagcg acagaacag ttaattaca gcagcgagt 
      tatcagaat aagtatagg cgcaagttg ctattcatg 
      gtactgata ggacttgca atagttgca ataattatt 
      ataatacta gttgtacag tttaaaaag tag
    mRNA sequence
    • >TC007177 RA
      tttttaaaa cacaaataa gattttttt tacaaaata 
      gcaggatta tgtggtagg gtcttgtta tcatctttt 
      tatattaca gtttgaagt attttttaa taaaaatcg 
      aatcgggtg tgatttttt taacatgta tttttaaaa 
      tgtgaggta tttcaatta ttcaacaat aagctaatt 
      ttgtttcat ccccgaggc cacaattga actcacccc 
      cttgttata acaataagt cacctgatc tttcctact 
      cctaacctc agttttaag tttacgaaa cgtcaaatc 
      acaaacgaa aaggggcgt cgaagacaa caatgagta 
      aacatccct acaattacg gtgctactg cgtccactg 
      acgtaggtt tcatcggta agcgctccc ccgaattca 
      ataatttat gcgatggcg tcgtaacaa acatttaca 
      caatcaact caagtctca agaccttag acaacgctt 
      taaaaacaa tcggaacca gaaaagaca atcagggat 
      taagaagtt caatacacg tcactcaat tgagcacaa 
      accagatcg catcagtca caagcaaag acattcaca 
      agctcaagc aacttgtat ccaaaagcg agaaacagc 
      agcagttgc aagtagaaa agttagaag agaatttta 
      aagaagcga tcacgcgct actattcct tacaaaagg 
      acttggcca acaagcaga aatcacatt tattggttt 
      ctgtaagca tagaaaatg actacacgc cggaagaag 
      acacagaac agcaacggc aagctcagc ttgcacgcg 
      aaatggcgt ttgagcaag atatgctca tggagagag 
      aagctagga ttaaacaaa ttgaagccg atgtacttg 
      atgttaacg aaatcatgc gcgaattgg ggtccttag 
      tgcacgccc aagcggaaa caatcgata ctattgaaa 
      atagcatag accatgcaa ctggcaatg tggaagaag 
      cgacagaac agttaatta cagcagcga gttatcaga 
      ataagtata ggcgcaagt tgctattca tggtactga 
      taggacttg caatagttg caataatta ttataatac 
      tagttgtac agtttaaaa agtagctgc gtaatatta 
      cttttcttt ataattact caaacctgt ttttgtggt 
      gaataactg cttgatata tttatatac agtatacac 
      agacttttt atagcaact aatcttatc atagaattt 
      atatttatt tatgtaatt atcttaatc acaaaatat 
      gaatgatat ttagttttt ttgtataat tatccataa 
      aattctatg agaaccgca aaaaataag tagtgacat 
      gaaacactc tgtagttaa aatgtag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY