Status
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Gene information
General information
TC008255 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7376074..7378498
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008255
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08148

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    dorsal ridge row bristle dorsal present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008255 RA
      MSAHVVCVT SGGGLPIFS RKKGNTDSL PFSTIGSLN 
      GVHMFGKSQ KITLQNTVT EDYSLAWKE YHDSLVLIG 
      ISSGCTIEV LTKTLDSIF NAIVLIVGI EEIKSQRNI 
      ERFKRELRI CYPLIDRLL DSLDCGDSG SRHSSDLIG 
      LVETILCPE NHLIQIVLD SFTECVDSL FSCVLIHGK 
      IAAATNSWW SLHPEEIKL LAILAQSEN TSSSKDIPV 
      FLPYKSPTV AFRFVCCTL IPDIQLCCL CGPTPVLSE 
      VEFSATNCF KNSADILSS AIQCHPRNF HSTCHIDGG 
      ILGLLLVNT TYGKYMITR NPHQNSNKK NASSSHRLD 
      ILRTFFYQS VLTFLVPSE DSNEDAEIS NKSEANVGL 
      ETYWCSEYH KCFAMKIRD NILAVLFNS SIPTFAMRL 
      ITEKTLKNL ISDKQICW
    CDS sequence
    • >TC008255 RA
      atgtcggca catgttgtt tgtgtcacc tcggggggt 
      ggtctgccc atattttcc aggaagaaa gggaatacg 
      gacagtctg cccttttca accattggg tccctaaat 
      ggggtccac atgtttggc aaatcgcaa aaaatcacc 
      cttcaaaat acggtaact gaagactac tctttagcc 
      tggaaggag taccacgac agcttggtt ttgataggg 
      atatcaagt ggatgcacc atcgaggtg ctcaccaag 
      accctcgac tcgattttc aacgcaatc gtgttaatt 
      gtcggaatc gaggaaatc aagtcgcag cgaaatatc 
      gaacggttt aagagagaa ttgcggatt tgctaccct 
      ttgatcgat cgcctgctg gattcttta gactgtggc 
      gattctggc agcagacac tcgtcggat ttaatcgga 
      ctggtggaa acgatttta tgtcccgaa aaccacttg 
      attcagatc gttcttgac tcgtttacc gaatgcgtc 
      gattcctta tttagctgc gtccttatt catggcaaa 
      attgcggct gcgaccaac agctggtgg tcgctgcac 
      ccagaagag atcaagctt ttggccatt ctggcccag 
      agcgagaac acgtcgagt tcgaaagac atccccgtt 
      ttcctgcca tacaaaagc ccaaccgtg gcttttcgt 
      ttcgtttgc tgtacttta attccggac atccagctg 
      tgttgtctc tgcggcccc actcccgtg ctctcggag 
      gtggaattt tccgcgact aattgtttc aagaattcc 
      gcagatatt ttaagttcc gccatacaa tgccacccg 
      cgcaacttc cactcgact tgtcacatc gacggcggg 
      attttgggt ttactgttg gtgaacacg acttacggc 
      aaatacatg attacgaga aatccgcat cagaatagt 
      aataaaaag aacgcgagc agctcgcat cgcttggat 
      attttacga acgtttttc tatcaatcg gttttaacg 
      ttccttgtg ccgagcgag gactcgaat gaagatgcg 
      gaaatttcc aataaaagt gaagcgaat gtggggctt 
      gaaacctac tggtgctcg gagtatcat aaatgcttc 
      gcaatgaaa atcagggat aatattcta gcggtttta 
      ttcaactcg tcgattccg acgttcgca atgcgactg 
      attacggag aaaactctc aagaatttg atctcagat 
      aaacaaatt tgttggtga
    mRNA sequence
    • >TC008255 RA
      aataaccag agatttgtc atggtggcc cacagaggg 
      cgtcgactc gcttcaatc aaaacgctc cacagcgaa 
      atttacggc caataatcg gccaatttc agtgtttta 
      agcgccttt caggcccgc aatcgctgg gtcacccgg 
      atttccggt gatgtatca atgtttact tgtacaata 
      tttgcccta acccatgaa aaatgtcgg cacatgttg 
      tttgtgtca cctcggggg gtggtctgc ccatatttt 
      ccaggaaga aagggaata cggacagtc tgccctttt 
      caaccattg ggtccctaa atggggtcc acatgtttg 
      gcaaatcgc aaaaaatca cccttcaaa atacggtaa 
      ctgaagact actctttag cctggaagg agtaccacg 
      acagcttgg ttttgatag ggatatcaa gtggatgca 
      ccatcgagg tgctcacca agaccctcg actcgattt 
      tcaacgcaa tcgtgttaa ttgtcggaa tcgaggaaa 
      tcaagtcgc agcgaaata tcgaacggt ttaagagag 
      aattgcgga tttgctacc ctttgatcg atcgcctgc 
      tggattctt tagactgtg gcgattctg gcagcagac 
      actcgtcgg atttaatcg gactggtgg aaacgattt 
      tatgtcccg aaaaccact tgattcaga tcgttcttg 
      actcgttta ccgaatgcg tcgattcct tatttagct 
      gcgtcctta ttcatggca aaattgcgg ctgcgacca 
      acagctggt ggtcgctgc acccagaag agatcaagc 
      ttttggcca ttctggccc agagcgaga acacgtcga 
      gttcgaaag acatccccg ttttcctgc catacaaaa 
      gcccaaccg tggcttttc gtttcgttt gctgtactt 
      taattccgg acatccagc tgtgttgtc tctgcggcc 
      ccactcccg tgctctcgg aggtggaat tttccgcga 
      ctaattgtt tcaagaatt ccgcagata ttttaagtt 
      ccgccatac aatgccacc cgcgcaact tccactcga 
      cttgtcaca tcgacggcg ggattttgg gtttactgt 
      tggtgaaca cgacttacg gcaaataca tgattacga 
      gaaatccgc atcagaata gtaataaaa agaacgcga 
      gcagctcgc atcgcttgg atattttac gaacgtttt 
      tctatcaat cggttttaa cgttccttg tgccgagcg 
      aggactcga atgaagatg cggaaattt ccaataaaa 
      gtgaagcga atgtggggc ttgaaacct actggtgct 
      cggagtatc ataaatgct tcgcaatga aaatcaggg 
      ataatattc tagcggttt tattcaact cgtcgattc 
      cgacgttcg caatgcgac tgattacgg agaaaactc 
      tcaagaatt tgatctcag ataaacaaa tttgttggt 
      gatggttta atatatttt ttgcaatat ctagtctct 
      caattcctg gggtacggg tggcggcgt tgggggtgg 
      tttattgtt cttaactct gccagtgta actcccgaa 
      caaccaaac ggtgtaaga acgagctcg tccctgaaa 
      cctacggct ctcaaaatg agccgtctg tattaacct 
      gaacaaaat tagagtcac aacctgaaa aaaattata 
      ataaaacaa gtttgattt tgacggagt cctacatac 
      ctcaaggat aatatagag aattaaatt acccacaat 
      aatccatcc aactgtttg ttttttttc gcggatcat 
      ttcgtaaca tcctctcgt cgaaatgct caaattaaa 
      ttcaggagt gtacaaata tgcttttcc tgtgacttc 
      gatattgtg gtggataca gggagaaag tgatttcat 
      agaacaaca actaaacgg tgtgttacc atcagcagc 
      agaacttaa aaaccaatt tactaatta acgatgttt 
      ttataattg tcgctttct acgctagat taggatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY