| General information | |||
| TC032860
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 7360134..7364074 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032860 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08147 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032860 RA
MNWGYYSTS QKNCCLGMK DQKCMVPRG KSIGGSSAL NAVLYVRGN PQDYDRWEA LGNPGWSYK DVLPYFIKS ENSQIDGDP DYHGIGGFW NVEYSFPAS DLYENFIAA CDELNMTRL DYNGKGQIG TDRSQINIK HGKRQSLGT AFLDNARKR ANIDIITNA LVTKVIINP ESKEAQGVE FVTKEEKFA ATAVREVIL SAGAINSPQ ILMLSGVGP KKHLEELGI EVIEDLPVG ENLLEHPLF PGLVIQTNY TLPGTTMEI LLDQYLQGL GPLTSPAHV DSIGFLHTG DGPADLPTV EYLFIPPGG STLPILNRV YNYDDNLVY NFLSRINSR SDITVYLAL LHQKSKGRI TLQSTSPID FPLIDLNMF AEPEDVDNL IEGIEFVMN LTKTEAFKK INANLLNVP ICTEFTKYS KQYWECMIR QMAQTIYHA CGTTAMGPN KTSSVVDSN LKVHGIGKL RVVDAGVFP TTISGHTNA PAVMVAEKI ADVIKNEYG NPGWSYKDV LPYFTKSEN SQIDGDPDY HGIGGFWNV EYSFPASDL YENFITACD ELNMTRLDY NGKKQIGFD KSQINTKHG KRQSLGTAF LDNARKRKN IDVVTNALV TKIIINPQS KEAKGVEFV TKNKKYSAT ATAGAVNSP QILMLSGVG PKKHLEELG IEVIEDLPV GENLLDHPL FPGLVIQTN YTLPDTTIK MLLEQYLKG QGPLTSSML KTIAFIHTG DGPEDLPTV EYLFIPPSG PTQPILKRI YNYDVNLAL NFLSRINSR SDITVYLTL LHQKSKGRI TLQSKNPID FPLVDLNMF AEAEDIDNL IEGIEFVMN LTKTEAFKK INAKLLDVP ICSDFTKHS RQYWECMIR QMAQTIYHT CGTTAMGPN KTTSVVDRD LKVHGIGKL RVVSAAVFP TTISGHANA PAVMVAEKI ADAIKKEYA
- >TC032860 RA
atgaactgg ggatactac agcacgtct caaaaaaat tgttgtttg ggaatgaaa gatcagaaa tgtatggtc cctcgggga aaatcaata gggggtagc agtgcccta aatgctgtg ctctacgtt agggggaat ccgcaagat tatgaccgt tgggaagct ttaggaaac cctggatgg tcgtacaaa gacgtgctt ccatacttc atcaaatcg gaaaattcg caaattgac ggcgatccg gattatcac ggaatcgga ggattttgg aacgttgaa tattcattt ccagcctca gatctttat gaaaatttt atcgctgcg tgtgatgag ttaaatatg acacgtctt gattacaat gggaaagga caaatcggt actgatagg tcccaaatc aatattaaa cacgggaaa aggcaaagt ttgggtaca gctttcttg gacaatgcc cgaaaacgg gcaaacatt gatatcata actaatgcc ctagtgacc aaagttata ataaaccca gaaagcaaa gaagcacaa ggggttgaa tttgtcacc aaagaagaa aaattcgca gcaacggct gtcagagaa gtcatactt tcagcaggt gccataaat tcgccccaa attttaatg ctctcaggg gtaggtccg aaaaaacat ttagaagag ttgggtatt gaagtaatt gaagatttg ccagttggt gagaattta ctcgagcac ccccttttt cccggtctg gtaatacaa accaattac acgctccct ggtacaaca atggaaata ttacttgac cagtatttg caagggcta ggaccgttg acaagtccg gcccatgtg gacagcatc ggcttcctc cacactgga gacggccct gccgacctg cccactgtc gaatattta tttattcct cccggtggc tcaaccctc ccaatttta aaccgagtt tacaattac gacgacaac ctcgtctac aacttcttg agccgaatc aactcacgc agcgacatt acggtatat ttggccctc ttgcaccaa aaatccaaa ggaagaatc actttacaa tcaacgagt cccatcgat ttccctctc atcgacttg aacatgttt gcagagccc gaagacgtt gataattta attgaagga atcgagttc gttatgaac ttaacaaaa accgaagct ttcaaaaaa atcaatgcc aatctcctt aatgtaccg atttgtacg gagtttacc aagtattcg aaacaatac tgggagtgt atgattcgt caaatggca caaaccatc tatcatgca tgtggcaca acagcgatg ggaccgaat aaaacatct tccgttgtc gatagtaat ctaaaagtt catggaatt gggaagttg agggtggtg gatgctggc gtgttccca acaacaatc tcggggcac accaatgcg cccgctgtt atggttgca gagaaaatt gcagatgtt attaaaaac gaatacggt aacccaggt tggtcgtac aaagacgtg cttccatac ttcaccaaa tcggaaaat tcgcaaatt gacggcgat ccagattat cacggaatt gggggtttc tggaacgtt gaatattca tttccagct tcagatctt tatgaaaat tttatcact gcgtgtgat gagttaaat atgacacgt cttgattat aacggtaaa aaacaaatc ggttttgat aaatcacaa atcaataca aaacatggg aaaagacaa agtttgggt accgctttc ctagataat gcccgaaaa cggaaaaac atcgatgtc gtaactaac gctctagtg accaaaatt ataataaac ccacaaagt aaagaagca aaaggggtt gaatttgtc accaaaaac aaaaaatac tcggcaacg gcaacggca ggggcggta aattcgccc caaatctta atgttgtca ggggtaggt cccaaaaaa catttagaa gagttgggt attgaagta attgaggac ttgccagtt ggtgagaac ttactcgac catcccctt tttcccggt ctggtaata caaaccaat tacacactt cccgataca acaatcaaa atgttgcta gaacaatat ttaaaaggg caagggccg ttaacaagt tcaatgctg aaaaccatc gccttcatt cacactggg gacggtcct gaggacctc cccaccgtt gaatacttg tttattcct ccgagcggc cccactcag ccaatttta aaacgaatt tacaattac gacgtcaac ctcgccctc aacttcttg agcagaatc aactcacgg agcgacatt accgtatac ttgaccctc ttgcatcaa aaatccaaa gggagaatc actttacaa tcaaagaat ccaatcgac tttcctctc gtcgacttg aacatgttt gcagaagca gaagacatt gataattta attgaagga atcgagttc gttatgaac ttaacaaaa accgaagct ttcaaaaaa atcaatgcc aagctcctc gatgtaccc atttgtagc gactttacc aagcattcg agacaatac tgggagtgc atgattcgt caaatggca caaactatt tatcatacg tgtggcacc accgcaatg gggccaaat aagacaact tccgttgtc gatagagat cttaaagtc cacggaatt gggaagttg agggttgtt agtgccgca gtttttcca acgaccatt tcgggacac gcaaatgca cccgctgtt atggtcgca gagaaaatt gccgatgca attaaaaaa gaatacgct taa
- >TC032860 RA
tcaaaccta attaccgaa cgaaatgct gaagcattt gtttaccag cttcaaatt caaataaaa caacggatt tgggaaatt atgacttta ttatagtgg gagctgggt cttcgggga gtgttttag caacaagac tcacagaga taagtgact ggaaaattc tcttgttgg aagcaggag gtgaagaag atgatttta gtaatattc ctgcagagc tcatgtttc ttcagctca gtgatatga actggggat actacagca cgtctcaaa aaaattgtt gtttgggaa tgaaagatc agaaatgta tggtccctc ggggaaaat caatagggg gtagcagtg ccctaaatg ctgtgctct acgttaggg ggaatccgc aagattatg accgttggg aagctttag gaaaccctg gatggtcgt acaaagacg tgcttccat acttcatca aatcggaaa attcgcaaa ttgacggcg atccggatt atcacggaa tcggaggat tttggaacg ttgaatatt catttccag cctcagatc tttatgaaa attttatcg ctgcgtgtg atgagttaa atatgacac gtcttgatt acaatggga aaggacaaa tcggtactg ataggtccc aaatcaata ttaaacacg ggaaaaggc aaagtttgg gtacagctt tcttggaca atgcccgaa aacgggcaa acattgata tcataacta atgccctag tgaccaaag ttataataa acccagaaa gcaaagaag cacaagggg ttgaatttg tcaccaaag aagaaaaat tcgcagcaa cggctgtca gagaagtca tactttcag caggtgcca taaattcgc cccaaattt taatgctct caggggtag gtccgaaaa aacatttag aagagttgg gtattgaag taattgaag atttgccag ttggtgaga atttactcg agcaccccc tttttcccg gtctggtaa tacaaacca attacacgc tccctggta caacaatgg aaatattac ttgaccagt atttgcaag ggctaggac cgttgacaa gtccggccc atgtggaca gcatcggct tcctccaca ctggagacg gccctgccg acctgccca ctgtcgaat atttattta ttcctcccg gtggctcaa ccctcccaa ttttaaacc gagtttaca attacgacg acaacctcg tctacaact tcttgagcc gaatcaact cacgcagcg acattacgg tatatttgg ccctcttgc accaaaaat ccaaaggaa gaatcactt tacaatcaa cgagtccca tcgatttcc ctctcatcg acttgaaca tgtttgcag agcccgaag acgttgata atttaattg aaggaatcg agttcgtta tgaacttaa caaaaaccg aagctttca aaaaaatca atgccaatc tccttaatg taccgattt gtacggagt ttaccaagt attcgaaac aatactggg agtgtatga ttcgtcaaa tggcacaaa ccatctatc atgcatgtg gcacaacag cgatgggac cgaataaaa catcttccg ttgtcgata gtaatctaa aagttcatg gaattggga agttgaggg tggtggatg ctggcgtgt tcccaacaa caatctcgg ggcacacca atgcgcccg ctgttatgg ttgcagaga aaattgcag atgttatta aaaacgaat acggtaacc caggttggt cgtacaaag acgtgcttc catacttca ccaaatcgg aaaattcgc aaattgacg gcgatccag attatcacg gaattgggg gtttctgga acgttgaat attcatttc cagcttcag atctttatg aaaatttta tcactgcgt gtgatgagt taaatatga cacgtcttg attataacg gtaaaaaac aaatcggtt ttgataaat cacaaatca atacaaaac atgggaaaa gacaaagtt tgggtaccg ctttcctag ataatgccc gaaaacgga aaaacatcg atgtcgtaa ctaacgctc tagtgacca aaattataa taaacccac aaagtaaag aagcaaaag gggttgaat ttgtcacca aaaacaaaa aatactcgg caacggcaa cggcagggg cggtaaatt cgccccaaa tcttaatgt tgtcagggg taggtccca aaaaacatt tagaagagt tgggtattg aagtaattg aggacttgc cagttggtg agaacttac tcgaccatc ccctttttc ccggtctgg taatacaaa ccaattaca cacttcccg atacaacaa tcaaaatgt tgctagaac aatatttaa aagggcaag ggccgttaa caagttcaa tgctgaaaa ccatcgcct tcattcaca ctggggacg gtcctgagg acctcccca ccgttgaat acttgttta ttcctccga gcggcccca ctcagccaa ttttaaaac gaatttaca attacgacg tcaacctcg ccctcaact tcttgagca gaatcaact cacggagcg acattaccg tatacttga ccctcttgc atcaaaaat ccaaaggga gaatcactt tacaatcaa agaatccaa tcgactttc ctctcgtcg acttgaaca tgtttgcag aagcagaag acattgata atttaattg aaggaatcg agttcgtta tgaacttaa caaaaaccg aagctttca aaaaaatca atgccaagc tcctcgatg tacccattt gtagcgact ttaccaagc attcgagac aatactggg agtgcatga ttcgtcaaa tggcacaaa ctatttatc atacgtgtg gcaccaccg caatggggc caaataaga caacttccg ttgtcgata gagatctta aagtccacg gaattggga agttgaggg ttgttagtg ccgcagttt ttccaacga ccatttcgg gacacgcaa atgcacccg ctgttatgg tcgcagaga aaattgccg atgcaatta aaaaagaat acgcttaat agtgttatt tattttaat aaagtatta atcttcaa
