Status
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Gene information
General information
TC032860
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008254,TC008253
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7360134..7364074
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032860
    iBeetle screen iB_08147

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032860 RA
      MNWGYYSTS QKNCCLGMK DQKCMVPRG KSIGGSSAL 
      NAVLYVRGN PQDYDRWEA LGNPGWSYK DVLPYFIKS 
      ENSQIDGDP DYHGIGGFW NVEYSFPAS DLYENFIAA 
      CDELNMTRL DYNGKGQIG TDRSQINIK HGKRQSLGT 
      AFLDNARKR ANIDIITNA LVTKVIINP ESKEAQGVE 
      FVTKEEKFA ATAVREVIL SAGAINSPQ ILMLSGVGP 
      KKHLEELGI EVIEDLPVG ENLLEHPLF PGLVIQTNY 
      TLPGTTMEI LLDQYLQGL GPLTSPAHV DSIGFLHTG 
      DGPADLPTV EYLFIPPGG STLPILNRV YNYDDNLVY 
      NFLSRINSR SDITVYLAL LHQKSKGRI TLQSTSPID 
      FPLIDLNMF AEPEDVDNL IEGIEFVMN LTKTEAFKK 
      INANLLNVP ICTEFTKYS KQYWECMIR QMAQTIYHA 
      CGTTAMGPN KTSSVVDSN LKVHGIGKL RVVDAGVFP 
      TTISGHTNA PAVMVAEKI ADVIKNEYG NPGWSYKDV 
      LPYFTKSEN SQIDGDPDY HGIGGFWNV EYSFPASDL 
      YENFITACD ELNMTRLDY NGKKQIGFD KSQINTKHG 
      KRQSLGTAF LDNARKRKN IDVVTNALV TKIIINPQS 
      KEAKGVEFV TKNKKYSAT ATAGAVNSP QILMLSGVG 
      PKKHLEELG IEVIEDLPV GENLLDHPL FPGLVIQTN 
      YTLPDTTIK MLLEQYLKG QGPLTSSML KTIAFIHTG 
      DGPEDLPTV EYLFIPPSG PTQPILKRI YNYDVNLAL 
      NFLSRINSR SDITVYLTL LHQKSKGRI TLQSKNPID 
      FPLVDLNMF AEAEDIDNL IEGIEFVMN LTKTEAFKK 
      INAKLLDVP ICSDFTKHS RQYWECMIR QMAQTIYHT 
      CGTTAMGPN KTTSVVDRD LKVHGIGKL RVVSAAVFP 
      TTISGHANA PAVMVAEKI ADAIKKEYA
    CDS sequence
    • >TC032860 RA
      atgaactgg ggatactac agcacgtct caaaaaaat 
      tgttgtttg ggaatgaaa gatcagaaa tgtatggtc 
      cctcgggga aaatcaata gggggtagc agtgcccta 
      aatgctgtg ctctacgtt agggggaat ccgcaagat 
      tatgaccgt tgggaagct ttaggaaac cctggatgg 
      tcgtacaaa gacgtgctt ccatacttc atcaaatcg 
      gaaaattcg caaattgac ggcgatccg gattatcac 
      ggaatcgga ggattttgg aacgttgaa tattcattt 
      ccagcctca gatctttat gaaaatttt atcgctgcg 
      tgtgatgag ttaaatatg acacgtctt gattacaat 
      gggaaagga caaatcggt actgatagg tcccaaatc 
      aatattaaa cacgggaaa aggcaaagt ttgggtaca 
      gctttcttg gacaatgcc cgaaaacgg gcaaacatt 
      gatatcata actaatgcc ctagtgacc aaagttata 
      ataaaccca gaaagcaaa gaagcacaa ggggttgaa 
      tttgtcacc aaagaagaa aaattcgca gcaacggct 
      gtcagagaa gtcatactt tcagcaggt gccataaat 
      tcgccccaa attttaatg ctctcaggg gtaggtccg 
      aaaaaacat ttagaagag ttgggtatt gaagtaatt 
      gaagatttg ccagttggt gagaattta ctcgagcac 
      ccccttttt cccggtctg gtaatacaa accaattac 
      acgctccct ggtacaaca atggaaata ttacttgac 
      cagtatttg caagggcta ggaccgttg acaagtccg 
      gcccatgtg gacagcatc ggcttcctc cacactgga 
      gacggccct gccgacctg cccactgtc gaatattta 
      tttattcct cccggtggc tcaaccctc ccaatttta 
      aaccgagtt tacaattac gacgacaac ctcgtctac 
      aacttcttg agccgaatc aactcacgc agcgacatt 
      acggtatat ttggccctc ttgcaccaa aaatccaaa 
      ggaagaatc actttacaa tcaacgagt cccatcgat 
      ttccctctc atcgacttg aacatgttt gcagagccc 
      gaagacgtt gataattta attgaagga atcgagttc 
      gttatgaac ttaacaaaa accgaagct ttcaaaaaa 
      atcaatgcc aatctcctt aatgtaccg atttgtacg 
      gagtttacc aagtattcg aaacaatac tgggagtgt 
      atgattcgt caaatggca caaaccatc tatcatgca 
      tgtggcaca acagcgatg ggaccgaat aaaacatct 
      tccgttgtc gatagtaat ctaaaagtt catggaatt 
      gggaagttg agggtggtg gatgctggc gtgttccca 
      acaacaatc tcggggcac accaatgcg cccgctgtt 
      atggttgca gagaaaatt gcagatgtt attaaaaac 
      gaatacggt aacccaggt tggtcgtac aaagacgtg 
      cttccatac ttcaccaaa tcggaaaat tcgcaaatt 
      gacggcgat ccagattat cacggaatt gggggtttc 
      tggaacgtt gaatattca tttccagct tcagatctt 
      tatgaaaat tttatcact gcgtgtgat gagttaaat 
      atgacacgt cttgattat aacggtaaa aaacaaatc 
      ggttttgat aaatcacaa atcaataca aaacatggg 
      aaaagacaa agtttgggt accgctttc ctagataat 
      gcccgaaaa cggaaaaac atcgatgtc gtaactaac 
      gctctagtg accaaaatt ataataaac ccacaaagt 
      aaagaagca aaaggggtt gaatttgtc accaaaaac 
      aaaaaatac tcggcaacg gcaacggca ggggcggta 
      aattcgccc caaatctta atgttgtca ggggtaggt 
      cccaaaaaa catttagaa gagttgggt attgaagta 
      attgaggac ttgccagtt ggtgagaac ttactcgac 
      catcccctt tttcccggt ctggtaata caaaccaat 
      tacacactt cccgataca acaatcaaa atgttgcta 
      gaacaatat ttaaaaggg caagggccg ttaacaagt 
      tcaatgctg aaaaccatc gccttcatt cacactggg 
      gacggtcct gaggacctc cccaccgtt gaatacttg 
      tttattcct ccgagcggc cccactcag ccaatttta 
      aaacgaatt tacaattac gacgtcaac ctcgccctc 
      aacttcttg agcagaatc aactcacgg agcgacatt 
      accgtatac ttgaccctc ttgcatcaa aaatccaaa 
      gggagaatc actttacaa tcaaagaat ccaatcgac 
      tttcctctc gtcgacttg aacatgttt gcagaagca 
      gaagacatt gataattta attgaagga atcgagttc 
      gttatgaac ttaacaaaa accgaagct ttcaaaaaa 
      atcaatgcc aagctcctc gatgtaccc atttgtagc 
      gactttacc aagcattcg agacaatac tgggagtgc 
      atgattcgt caaatggca caaactatt tatcatacg 
      tgtggcacc accgcaatg gggccaaat aagacaact 
      tccgttgtc gatagagat cttaaagtc cacggaatt 
      gggaagttg agggttgtt agtgccgca gtttttcca 
      acgaccatt tcgggacac gcaaatgca cccgctgtt 
      atggtcgca gagaaaatt gccgatgca attaaaaaa 
      gaatacgct taa
    mRNA sequence
    • >TC032860 RA
      tcaaaccta attaccgaa cgaaatgct gaagcattt 
      gtttaccag cttcaaatt caaataaaa caacggatt 
      tgggaaatt atgacttta ttatagtgg gagctgggt 
      cttcgggga gtgttttag caacaagac tcacagaga 
      taagtgact ggaaaattc tcttgttgg aagcaggag 
      gtgaagaag atgatttta gtaatattc ctgcagagc 
      tcatgtttc ttcagctca gtgatatga actggggat 
      actacagca cgtctcaaa aaaattgtt gtttgggaa 
      tgaaagatc agaaatgta tggtccctc ggggaaaat 
      caatagggg gtagcagtg ccctaaatg ctgtgctct 
      acgttaggg ggaatccgc aagattatg accgttggg 
      aagctttag gaaaccctg gatggtcgt acaaagacg 
      tgcttccat acttcatca aatcggaaa attcgcaaa 
      ttgacggcg atccggatt atcacggaa tcggaggat 
      tttggaacg ttgaatatt catttccag cctcagatc 
      tttatgaaa attttatcg ctgcgtgtg atgagttaa 
      atatgacac gtcttgatt acaatggga aaggacaaa 
      tcggtactg ataggtccc aaatcaata ttaaacacg 
      ggaaaaggc aaagtttgg gtacagctt tcttggaca 
      atgcccgaa aacgggcaa acattgata tcataacta 
      atgccctag tgaccaaag ttataataa acccagaaa 
      gcaaagaag cacaagggg ttgaatttg tcaccaaag 
      aagaaaaat tcgcagcaa cggctgtca gagaagtca 
      tactttcag caggtgcca taaattcgc cccaaattt 
      taatgctct caggggtag gtccgaaaa aacatttag 
      aagagttgg gtattgaag taattgaag atttgccag 
      ttggtgaga atttactcg agcaccccc tttttcccg 
      gtctggtaa tacaaacca attacacgc tccctggta 
      caacaatgg aaatattac ttgaccagt atttgcaag 
      ggctaggac cgttgacaa gtccggccc atgtggaca 
      gcatcggct tcctccaca ctggagacg gccctgccg 
      acctgccca ctgtcgaat atttattta ttcctcccg 
      gtggctcaa ccctcccaa ttttaaacc gagtttaca 
      attacgacg acaacctcg tctacaact tcttgagcc 
      gaatcaact cacgcagcg acattacgg tatatttgg 
      ccctcttgc accaaaaat ccaaaggaa gaatcactt 
      tacaatcaa cgagtccca tcgatttcc ctctcatcg 
      acttgaaca tgtttgcag agcccgaag acgttgata 
      atttaattg aaggaatcg agttcgtta tgaacttaa 
      caaaaaccg aagctttca aaaaaatca atgccaatc 
      tccttaatg taccgattt gtacggagt ttaccaagt 
      attcgaaac aatactggg agtgtatga ttcgtcaaa 
      tggcacaaa ccatctatc atgcatgtg gcacaacag 
      cgatgggac cgaataaaa catcttccg ttgtcgata 
      gtaatctaa aagttcatg gaattggga agttgaggg 
      tggtggatg ctggcgtgt tcccaacaa caatctcgg 
      ggcacacca atgcgcccg ctgttatgg ttgcagaga 
      aaattgcag atgttatta aaaacgaat acggtaacc 
      caggttggt cgtacaaag acgtgcttc catacttca 
      ccaaatcgg aaaattcgc aaattgacg gcgatccag 
      attatcacg gaattgggg gtttctgga acgttgaat 
      attcatttc cagcttcag atctttatg aaaatttta 
      tcactgcgt gtgatgagt taaatatga cacgtcttg 
      attataacg gtaaaaaac aaatcggtt ttgataaat 
      cacaaatca atacaaaac atgggaaaa gacaaagtt 
      tgggtaccg ctttcctag ataatgccc gaaaacgga 
      aaaacatcg atgtcgtaa ctaacgctc tagtgacca 
      aaattataa taaacccac aaagtaaag aagcaaaag 
      gggttgaat ttgtcacca aaaacaaaa aatactcgg 
      caacggcaa cggcagggg cggtaaatt cgccccaaa 
      tcttaatgt tgtcagggg taggtccca aaaaacatt 
      tagaagagt tgggtattg aagtaattg aggacttgc 
      cagttggtg agaacttac tcgaccatc ccctttttc 
      ccggtctgg taatacaaa ccaattaca cacttcccg 
      atacaacaa tcaaaatgt tgctagaac aatatttaa 
      aagggcaag ggccgttaa caagttcaa tgctgaaaa 
      ccatcgcct tcattcaca ctggggacg gtcctgagg 
      acctcccca ccgttgaat acttgttta ttcctccga 
      gcggcccca ctcagccaa ttttaaaac gaatttaca 
      attacgacg tcaacctcg ccctcaact tcttgagca 
      gaatcaact cacggagcg acattaccg tatacttga 
      ccctcttgc atcaaaaat ccaaaggga gaatcactt 
      tacaatcaa agaatccaa tcgactttc ctctcgtcg 
      acttgaaca tgtttgcag aagcagaag acattgata 
      atttaattg aaggaatcg agttcgtta tgaacttaa 
      caaaaaccg aagctttca aaaaaatca atgccaagc 
      tcctcgatg tacccattt gtagcgact ttaccaagc 
      attcgagac aatactggg agtgcatga ttcgtcaaa 
      tggcacaaa ctatttatc atacgtgtg gcaccaccg 
      caatggggc caaataaga caacttccg ttgtcgata 
      gagatctta aagtccacg gaattggga agttgaggg 
      ttgttagtg ccgcagttt ttccaacga ccatttcgg 
      gacacgcaa atgcacccg ctgttatgg tcgcagaga 
      aaattgccg atgcaatta aaaaagaat acgcttaat 
      agtgttatt tattttaat aaagtatta atcttcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY