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Gene information
General information
TC007227 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7228813..7234201
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007227
    iBeetle screen iB_08146

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007227 RA
      MSQSILRLL KREASNIAA STGNSTTNI TLPAVSSGG 
      TVPETVVKL EGSTTEFST TPPDVTTTG PALKDRAME 
      MAEVVAEKT GMKPWMVIT IVIAICAII LGICFCCIR 
      RCFRKRRAK DGKKGMKGV DLKSVQLLG SSYKEKVQP 
      DMDELTENA EEPDEAEKP EVQKLGKLQ YKLEYDFNQ 
      NSLSVTVIQ AEDLPALDM GGTSDPYVK VYLLPDKKK 
      KFETKVHRK TLSPVFNET FVFKNIPYA DAMNKTLVF 
      AIFDFDRFS KHDQIGEVK VPLCQIDLA QTIEEWREL 
      QSVEGEGGQ ENKLGDICF SLRYVPTAG KLTVVILEA 
      KNLKKMDVG GLSDPYVKI ALVQNGKRL KKKKTSIKK 
      CTLNPYYNE SFTFEVPFE QIQKVNLVV TVVDYDRIG 
      TSEPIGKVV LGYNASGTE LRHWSDMLA SPRRPIAQW 
      HTLKDPEDE KKD
    CDS sequence
    • >TC007227 RA
      atgtctcaa tcgatatta agattacta aagcgggag 
      gcgagcaat atagccgcc tccaccgga aatagtacc 
      acaaacata acacttccg gcagtttca agtggaggc 
      acagtacca gaaacagta gtcaaactg gaaggatca 
      actacggaa ttttcaaca actccgcct gatgttacc 
      acaacagga ccagcactt aaagatcga gccatggag 
      atggcagag gttgtagct gaaaagact ggaatgaag 
      ccatggatg gtcatcacc atcgtgatt gctatttgt 
      gctataatc cttggaatc tgcttctgc tgcataaga 
      agatgtttc cggaaaagg agagcaaag gatgggaag 
      aaaggtatg aaaggagtg gacttgaag tcagtccag 
      ttgcttggc tcctcgtac aaagagaaa gtccaaccg 
      gacatggac gaactgacg gaaaatgct gaagagccg 
      gacgaggcg gaaaagcca gaggtgcaa aaactgggc 
      aaactgcag tataagctc gaatatgac ttcaatcaa 
      aacagtcta tccgtcact gtcattcag gccgaagat 
      ttaccagct cttgacatg ggtggcact tcagatccc 
      tacgtcaag gtgtacttg cttccagac aagaagaag 
      aagttcgaa acaaaagtc catcgtaaa acgttaagt 
      ccagttttc aatgaaacg tttgtcttt aagaacatt 
      ccgtacgcc gatgcaatg aacaaaacc ctcgtgttt 
      gcgattttc gactttgat cggttctcc aaacatgac 
      cagatcggt gaggtgaaa gtccccctt tgtcaaatc 
      gatttagcc caaacgatc gaggaatgg cgcgagctg 
      cagagcgtg gaaggagaa ggtggtcag gaaaacaag 
      ttgggagac atttgcttc tcattacga tacgtaccg 
      acagctgga aaactcacc gtcgttatc ctagaagct 
      aagaacttg aagaaaatg gacgttgga ggattatct 
      gacccgtac gtgaagatc gctctagta cagaacgga 
      aagaggcta aagaagaag aagaccagc atcaagaag 
      tgcacactg aacccgtat tacaatgag tcgttcact 
      tttgaagta ccgttcgaa caaattcag aaagttaac 
      ttggtggta actgtggtg gactacgac aggattggt 
      acctcggaa cccattggt aaagtcgtc ttgggttac 
      aacgcaagc gggaccgaa ctgcgccac tggtctgac 
      atgttagca tcgccgcgc aggcccatc gcccagtgg 
      cacaccctc aaagaccca gaggacgag aagaaggac 
      taa
    mRNA sequence
    • >TC007227 RA
      ggtggccat tttaccgac tcttgcgct cgtcctacc 
      ctcaagttg aggcagaaa ccactgcct gaaaagcca 
      agcagtgca cacaaaatg tcctacagt tcttgaaag 
      tggaaagtt tccgttctt gaggtccat ttaacaaaa 
      atacgaaat gtctcaatc gatattaag attactaaa 
      gcgggaggc gagcaatat agccgcctc caccggaaa 
      tagtaccac aaacataac acttccggc agtttcaag 
      tggaggcac agtaccaga aacagtagt caaactgga 
      aggatcaac tacggaatt ttcaacaac tccgcctga 
      tgttaccac aacaggacc agcacttaa agatcgagc 
      catggagat ggcagaggt tgtagctga aaagactgg 
      aatgaagcc atggatggt catcaccat cgtgattgc 
      tatttgtgc tataatcct tggaatctg cttctgctg 
      cataagaag atgtttccg gaaaaggag agcaaagga 
      tgggaagaa aggtatgaa aggagtgga cttgaagtc 
      agtccagtt gcttggctc ctcgtacaa agagaaagt 
      ccaaccgga catggacga actgacgga aaatgctga 
      agagccgga cgaggcgga aaagccaga ggtgcaaaa 
      actgggcaa actgcagta taagctcga atatgactt 
      caatcaaaa cagtctatc cgtcactgt cattcaggc 
      cgaagattt accagctct tgacatggg tggcacttc 
      agatcccta cgtcaaggt gtacttgct tccagacaa 
      gaagaagaa gttcgaaac aaaagtcca tcgtaaaac 
      gttaagtcc agttttcaa tgaaacgtt tgtctttaa 
      gaacattcc gtacgccga tgcaatgaa caaaaccct 
      cgtgtttgc gattttcga ctttgatcg gttctccaa 
      acatgacca gatcggtga ggtgaaagt ccccctttg 
      tcaaatcga tttagccca aacgatcga ggaatggcg 
      cgagctgca gagcgtgga aggagaagg tggtcagga 
      aaacaagtt gggagacat ttgcttctc attacgata 
      cgtaccgac agctggaaa actcaccgt cgttatcct 
      agaagctaa gaacttgaa gaaaatgga cgttggagg 
      attatctga cccgtacgt gaagatcgc tctagtaca 
      gaacggaaa gaggctaaa gaagaagaa gaccagcat 
      caagaagtg cacactgaa cccgtatta caatgagtc 
      gttcacttt tgaagtacc gttcgaaca aattcagaa 
      agttaactt ggtggtaac tgtggtgga ctacgacag 
      gattggtac ctcggaacc cattggtaa agtcgtctt 
      gggttacaa cgcaagcgg gaccgaact gcgccactg 
      gtctgacat gttagcatc gccgcgcag gcccatcgc 
      ccagtggca caccctcaa agacccaga ggacgagaa 
      gaaggacta agctggtcg cttacaacg ctgatttat 
      tcaaacact ataagattt actcataaa gatattggt 
      tccctagca tgagaaaac gtcgcgtta cttaattta 
      cttaaaagg aaaacagtt ataagtgta tatttgtaa 
      catcataga tgtcagtaa catatattt tataataac 
      tacttgaag gaaatattc gatgtaact ctcgggact 
      acctaattt tattatttt tcccgaaaa caggttact 
      cagcttgcc ttcaacgaa ctactgttt ctgtaaata 
      gatcgcctt cttccaaac cgtttttcg tttcgaaat 
      tgtaacacc cacattgaa acagacgcg gaagagtcg 
      ccaatgagt attagacga ttagatcat acagacaat 
      agaaatttg atagcttgt agaacagtt gaaattact 
      ttgcaaatt ttaatggac cggagcaca aaaaacttt 
      cgaatctca atcccacaa tgggcgcta ttgcaattt 
      gtggattat tattgccta tttaccacc aaaacaacg 
      atacgacga caaaaggcg cattgttgg gattgcgtc 
      agcattgag acatgctgt tttaaacaa cgtagagta 
      gaattccgt tccaaaatt ttatttggc ggtaccctg 
      accaaaaat cacacggtc cagggtacc aaacagaaa 
      aaatgatat tattcttcc tcttttctt gtatatacc 
      tataaataa tgttacaat gttgtgtaa aataaaaaa 
      gtatgtaaa cagtataga gaagtgtta cggcccctc 
      ttatgtatg taccctccc aaacaccag tatatgggc 
      ccaagttat ttatttagt aagaaatca gcacacacc 
      tagctctaa taccgaaga aatttggta atcatcaat 
      gcgtgatct tattttgtt atcaggtgg tgacaattg 
      tagtttgca cgagcttct tggagtggc aatatctta 
      ggaatgtaa ttgtacgtg atcttaggt ttagttaaa 
      caaagaaaa caaaaactg taaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY