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Gene information
General information
TC008220 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6944430..6945904
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008220
    iBeetle screen iB_08142

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008220 RA
      MALNLQVKI HPVVLFQIV DAYERRNAD SHRVIGTLL 
      GNVDKGIVE VINCFCVPH KETVDQVEA ELNYAMDVH 
      DLNRRVNSN ESIVGWWAT GHEVTNHSS VIHEYYARE 
      CNNPVHVTL DSSLQGGRM GLKAYVCVS LGVPNGKQG 
      CMFTSIPID VTCYDPEIF GLQLCQKTL GAGGGRSRN 
      VHPLLDLAQ VSEAGSKMG ILLEQVLSY VEDVLAGKV 
      QPNNAVGRS LLDLINSVP HMNNEQFSE MFNSNVKDL 
      LMVITLSQL IKTQLQLNE KLTLLTSV
    CDS sequence
    • >TC008220 RA
      atggcgctc aacttgcaa gttaaaatc catccggtg 
      gtgcttttc caaattgtg gacgcctat gaacggaga 
      aatgcagac tcgcaccgg gttattggc acgctcttg 
      ggaaacgtc gataaaggc atcgtggag gtgataaac 
      tgcttttgc gtcccgcat aaggagacc gtcgaccag 
      gtcgaagcc gagctgaat tacgccatg gatgtgcat 
      gacctaaac cgtcgtgtg aattccaac gaaagtatc 
      gtcggttgg tgggccaca ggccacgaa gtcaccaac 
      cacagttcc gttattcac gagtattat gccagagaa 
      tgcaacaat ccagtccac gtgaccctt gattcaagt 
      cttcaaggg ggccgcatg ggccttaaa gcatacgtg 
      tgtgtatct ctaggagtc cccaatggg aaacaaggt 
      tgcatgttt acatccatc cccatcgat gttacgtgt 
      tacgacccc gagattttt gggttacaa ctatgtcaa 
      aaaacattg ggggctggg ggagggagg tctcggaac 
      gttcatccc ttgttggac ttggcccag gtgtctgaa 
      gctggatca aagatgggg atactttta gagcaagtt 
      ctgtcgtat gttgaagat gttttagct ggaaaggtt 
      caaccgaat aatgcagta gggaggtca ctactcgac 
      ttgatcaat tccgtgccg catatgaat aacgaacag 
      ttctcagaa atgtttaac agtaacgtc aaagacttg 
      ttaatggtg attacgttg tctcagctc attaaaacc 
      caattgcaa cttaacgag aagcttacg ctgctcacg 
      tcggtgtaa
    mRNA sequence
    • >TC008220 RA
      tttgtgaaa aatttgcac ccaccttct aagttttat 
      ccagattaa tccaacagg cacacgcta cggaacggt 
      ataaatccg gcacgcctg cattgcgga tataccaaa 
      acaacgcat atacacagc cagtggtgg gccgcgaaa 
      ccattttga tgcgagcca tcaatacta aaagtcaaa 
      cttggacgt gccgatttc gacactaat ccaccccca 
      aaccgcgaa agcgccaca ccacgcgaa acgaccacg 
      attttggga aaaacggcc gaaaacccg gtcgaattc 
      ggcggaatt tcgacgatt tatcgatga aacaaaaca 
      cgaactttc gcagatttg aatgcaaac tggacactt 
      ttcgcggtg ctgtgttgg catgcttgg aaacttagg 
      ggattgcgc atggttctt ggttacttt tctgggcaa 
      aatggcgct caacttgca agttaaaat ccatccggt 
      ggtgctttt ccaaattgt ggacgccta tgaacggag 
      aaatgcaga ctcgcaccg ggttattgg cacgctctt 
      gggaaacgt cgataaagg catcgtgga ggtgataaa 
      ctgcttttg cgtcccgca taaggagac cgtcgacca 
      ggtcgaagc cgagctgaa ttacgccat ggatgtgca 
      tgacctaaa ccgtcgtgt gaattccaa cgaaagtat 
      cgtcggttg gtgggccac aggccacga agtcaccaa 
      ccacagttc cgttattca cgagtatta tgccagaga 
      atgcaacaa tccagtcca cgtgaccct tgattcaag 
      tcttcaagg gggccgcat gggccttaa agcatacgt 
      gtgtgtatc tctaggagt ccccaatgg gaaacaagg 
      ttgcatgtt tacatccat ccccatcga tgttacgtg 
      ttacgaccc cgagatttt tgggttaca actatgtca 
      aaaaacatt gggggctgg gggagggag gtctcggaa 
      cgttcatcc cttgttgga cttggccca ggtgtctga 
      agctggatc aaagatggg gatactttt agagcaagt 
      tctgtcgta tgttgaaga tgttttagc tggaaaggt 
      tcaaccgaa taatgcagt agggaggtc actactcga 
      cttgatcaa ttccgtgcc gcatatgaa taacgaaca 
      gttctcaga aatgtttaa cagtaacgt caaagactt 
      gttaatggt gattacgtt gtctcagct cattaaaac 
      ccaattgca acttaacga gaagcttac gctgctcac 
      gtcggtgta atatgacaa aaataaacg aatcagaat 
      ttt
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