Status
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Gene information
General information
TC032846
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007259
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6747247..6749432
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032846
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08140

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032846 RA
      MKNFNSTEI GDELLIPVE GYIAAAVVL FCIGFFGFS 
      LNLTVIIFM LKERQLWSP LNIILFNLV VSDFLVSVL 
      GNPWTFFSA INYGWIFGE TGCTIYGFI MSLLGITSI 
      TTLTVLAFE RYLLIARPF RNNALNFHS AALSVFSIW 
      LYSLSLTIP PLIGWGEYV HEAANLSCS VNWEEKSPN 
      STSYILYLF AFGLFLPLV IITFSYVNI ILTMRRNAA 
      FRVGQVSKA ENKVAYMIF IMIIAFLTA WSPYAIMAL 
      IVQFGDAAL VTPGMAVIP ALLAKSSIC YNPVIYIGL 
      NAQFQQAWM QKWKKNRRG SDALGTSRV MLETIHQAC 
      RDEKTDKLL EKKTKFCKD FETDVSML
    CDS sequence
    • >TC032846 RA
      atgaagaat tttaattcg actgaaatc ggcgatgaa 
      ttactaatt cctgtggaa ggctacatt gcggcagct 
      gttgtgctg ttctgtatc gggtttttt gggttcagc 
      ctcaatcta actgttatt attttcatg ctgaaagag 
      cgccagtta tggtcaccg ctaaacata attctcttt 
      aatttagtt gttagcgat ttcctagtc tcggtgttg 
      ggaaatccg tggacgttt ttttcggcg attaattac 
      ggatggatt tttggcgag actgggtgt acgatttac 
      ggttttata atgtctctt ttaggtatc acgtcaatc 
      acgacttta accgttctt gcgttcgaa cgttattta 
      ttaatcgca cggccgttc cgtaacaat gccttaaat 
      ttccacagc gctgctctt tcagtcttc agtatttgg 
      ttgtattca ttaagtttg acaataccc cctttgatt 
      ggttggggt gaatacgtc catgaagcc gccaatctt 
      agctgttca gtaaactgg gaagagaaa agcccgaac 
      tccacgagt tacatttta tatttgttc gctttcggc 
      ctttttctc cctctcgta ataatcact ttcagttac 
      gtaaacatc attttgacg atgcgacga aacgccgcc 
      tttcgagtg ggtcaggtt tcgaaagcc gaaaacaaa 
      gtggcgtat atgattttc atcatgata attgctttt 
      ctaacagct tggtctccg tacgctatt atggctctt 
      attgtgcag tttggagat gctgctctt gtgactccg 
      ggcatggcg gtgattccg gcattgttg gccaagagc 
      agcatttgt tataatcct gtcatttac atcgggctg 
      aatgcacag ttccaacaa gcctggatg caaaaatgg 
      aaaaaaaat cgcagggga agcgacgcg ttaggaacg 
      tccagagtg atgctagag acgatacat caagcctgc 
      agagacgaa aaaaccgac aagctttta gagaaaaaa 
      actaagttt tgcaaggac tttgagact gatgtttcc 
      atgctttaa
    mRNA sequence
    • >TC032846 RA
      tcagtttga tagaggttt gcgtgtgcg aaagatgct 
      gattgttgc ccgcgctcg aaactcagt taagtgaag 
      tcacaattc cgcatgaag aattttaat tcgactgaa 
      atcggcgat gaattacta attcctgtg gaaggctac 
      attgcggca gctgttgtg ctgttctgt atcgggttt 
      tttgggttc agcctcaat ctaactgtt attattttc 
      atgctgaaa gagcgccag ttatggtca ccgctaaac 
      ataattctc tttaattta gttgttagc gatttccta 
      gtctcggtg ttgggaaat ccgtggacg tttttttcg 
      gcgattaat tacggatgg atttttggc gagactggg 
      tgtacgatt tacggtttt ataatgtct cttttaggt 
      atcacgtca atcacgact ttaaccgtt cttgcgttc 
      gaacgttat ttattaatc gcacggccg ttccgtaac 
      aatgcctta aatttccac agcgctgct ctttcagtc 
      ttcagtatt tggttgtat tcattaagt ttgacaata 
      ccccctttg attggttgg ggtgaatac gtccatgaa 
      gccgccaat cttagctgt tcagtaaac tgggaagag 
      aaaagcccg aactccacg agttacatt ttatatttg 
      ttcgctttc ggccttttt ctccctctc gtaataatc 
      actttcagt tacgtaaac atcattttg acgatgcga 
      cgaaacgcc gcctttcga gtgggtcag gtttcgaaa 
      gccgaaaac aaagtggcg tatatgatt ttcatcatg 
      ataattgct tttctaaca gcttggtct ccgtacgct 
      attatggct cttattgtg cagtttgga gatgctgct 
      cttgtgact ccgggcatg gcggtgatt ccggcattg 
      ttggccaag agcagcatt tgttataat cctgtcatt 
      tacatcggg ctgaatgca cagttccaa caagcctgg 
      atgcaaaaa tggaaaaaa aatcgcagg ggaagcgac 
      gcgttagga acgtccaga gtgatgcta gagacgata 
      catcaagcc tgcagagac gaaaaaacc gacaagctt 
      ttagagaaa aaaactaag ttttgcaag gactttgag 
      actgatgtt tccatgctt taaacaatt attgtacca 
      ctggataat aaaacattt tttttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY