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Gene information
General information
TC008191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6422479..6424333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008191
    iBeetle screen iB_08135

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008191 RA
      MRLSTPVIV AISGFCILL LALLCSSVI FPELENYLI 
      SKETAFVEG SKTFETWKK IPFPFKLKI YFLNVTNVD 
      DFQMGAKPT FREIGPYVY DEFREREVL AVSEDNDTV 
      RYNQKKTYF FNREDSGCR TEEDVVTII NTAAVGIAH 
      KIYKLAPDA MDIVNDALP FLYPGIKNI FVTNTVRNI 
      LFDGVTMSC GSDEVAMIC DGLKKRRPP SIRPADNNK 
      DYLVAMFHH MNGSVDGPY EMQRGLKDS SKGQVVGFK 
      DNNMLTLWT GDCNTIQGT DLTLNPNLN DLPPKIYFF 
      ASDFCRSFS VKFDKELVY LGLKSYKFK NSNLFHIEK 
      NCFCDKNPE NEVPGCTPA GTMDVSPCT GSSVVLSQP 
      HFLNAEKSL LDEAQGLAP NENRHGTFI IMEPKTGLA 
      LVVKTRFQM NVYLQDFED VDLLANVSA GFFPLLWLE 
      NGAEVPHDI VDEIRENFN KLMVFDAVK YFLIVLGAI 
      LLAVSIMLA MSQDKKWCF TNKNNSIAS DNQMAKIMN 
      LNANAALAR NWENINNGT WKIVE
    CDS sequence
    • >TC008191 RA
      atgcgccta agtacgccc gtaattgtg gccatttca 
      ggcttctgt atcttactc ttagcccta ctttgctca 
      agtgtgatt tttcctgaa ctggaaaac tacttaata 
      agtaaggaa accgcgttc gtggaaggg agcaagaca 
      tttgagacc tggaaaaaa ataccgttc ccattcaaa 
      ttaaaaatt tattttctc aatgtgacc aacgttgat 
      gatttccaa atgggggcc aaaccaact tttagggaa 
      atagggccc tatgtttac gacgaattt cgggaacgc 
      gaagtttta gcagttagt gaagacaac gacactgtt 
      agatataat cagaaaaag acctatttt ttcaataga 
      gaggactca gggtgtcgg acggaagaa gatgtagta 
      acgataatt aacactgcc gctgtggga atcgctcat 
      aaaatttat aaactcgca ccagacgcg atggatata 
      gtaaacgat gctttacca ttcctttac cctggtata 
      aaaaatatt tttgtgact aacacagtc cgaaacatt 
      ttattcgac ggtgttaca atgtcttgc ggatcggac 
      gaagtggca atgatttgt gatgggtta aagaaacga 
      cgtccaccc agcattcga ccagctgac aacaacaag 
      gactatttg gttgcaatg ttccatcat atgaatggg 
      tccgttgac ggtccttac gagatgcaa cgtgggctt 
      aaagactca agcaaaggt caggtggtc ggtttcaaa 
      gacaacaac atgttgacg ctctggacc ggcgactgc 
      aacacaatt caaggaacc gacctcacc ctcaaccca 
      aacttgaac gacttgccc ccgaaaata tattttttc 
      gcatctgac ttctgccgc tcattttcc gtaaaattc 
      gacaaagag ttggtttat ttggggctt aaaagttac 
      aaattcaag aacagtaat ttattccac attgagaaa 
      aattgtttc tgtgacaag aaccccgaa aatgaggtg 
      ccgggatgt acaccggct ggaacaatg gatgtgagt 
      ccttgtacc ggatcctcg gttgtactc tcgcagccg 
      cattttctg aacgctgaa aaatcgcta cttgacgag 
      gcccaaggc ctcgcacct aacgaaaat aggcatggg 
      acctttatc ataatggaa ccgaaaacc ggtttggca 
      cttgtcgtc aaaacacgg tttcaaatg aacgtttat 
      ttacaggat tttgaagat gttgaccta cttgcaaat 
      gttagcgcc ggctttttc ccattgctt tggctcgag 
      aatggcgct gaggttccc cacgatatc gtggacgag 
      attcgcgaa aatttcaac aaactgatg gtttttgac 
      gcggttaag tacttcctg atcgtgctc ggggcgatt 
      ttgttagcc gtttccatt atgctagcg atgagtcaa 
      gacaaaaag tggtgtttc accaacaaa aataactca 
      attgctagc gacaaccaa atggcgaaa attatgaat 
      ctgaacgca aatgctgca cttgcccgg aactgggaa 
      aatatcaac aacggcact tggaaaatc gtggaatag
    mRNA sequence
    • >TC008191 RA
      actagcacg caaaatgcg cctaagtac gcccgtaat 
      tgtggccat ttcaggctt ctgtatctt actcttagc 
      cctactttg ctcaagtgt gatttttcc tgaactgga 
      aaactactt aataagtaa ggaaaccgc gttcgtgga 
      agggagcaa gacatttga gacctggaa aaaaatacc 
      gttcccatt caaattaaa aatttattt tctcaatgt 
      gaccaacgt tgatgattt ccaaatggg ggccaaacc 
      aacttttag ggaaatagg gccctatgt ttacgacga 
      atttcggga acgcgaagt tttagcagt tagtgaaga 
      caacgacac tgttagata taatcagaa aaagaccta 
      ttttttcaa tagagagga ctcagggtg tcggacgga 
      agaagatgt agtaacgat aattaacac tgccgctgt 
      gggaatcgc tcataaaat ttataaact cgcaccaga 
      cgcgatgga tatagtaaa cgatgcttt accattcct 
      ttaccctgg tataaaaaa tatttttgt gactaacac 
      agtccgaaa cattttatt cgacggtgt tacaatgtc 
      ttgcggatc ggacgaagt ggcaatgat ttgtgatgg 
      gttaaagaa acgacgtcc acccagcat tcgaccagc 
      tgacaacaa caaggacta tttggttgc aatgttcca 
      tcatatgaa tgggtccgt tgacggtcc ttacgagat 
      gcaacgtgg gcttaaaga ctcaagcaa aggtcaggt 
      ggtcggttt caaagacaa caacatgtt gacgctctg 
      gaccggcga ctgcaacac aattcaagg aaccgacct 
      caccctcaa cccaaactt gaacgactt gcccccgaa 
      aatatattt tttcgcatc tgacttctg ccgctcatt 
      ttccgtaaa attcgacaa agagttggt ttatttggg 
      gcttaaaag ttacaaatt caagaacag taatttatt 
      ccacattga gaaaaattg tttctgtga caagaaccc 
      cgaaaatga ggtgccggg atgtacacc ggctggaac 
      aatggatgt gagtccttg taccggatc ctcggttgt 
      actctcgca gccgcattt tctgaacgc tgaaaaatc 
      gctacttga cgaggccca aggcctcgc acctaacga 
      aaataggca tgggacctt tatcataat ggaaccgaa 
      aaccggttt ggcacttgt cgtcaaaac acggtttca 
      aatgaacgt ttatttaca ggattttga agatgttga 
      cctacttgc aaatgttag cgccggctt tttcccatt 
      gctttggct cgagaatgg cgctgaggt tccccacga 
      tatcgtgga cgagattcg cgaaaattt caacaaact 
      gatggtttt tgacgcggt taagtactt cctgatcgt 
      gctcggggc gattttgtt agccgtttc cattatgct 
      agcgatgag tcaagacaa aaagtggtg tttcaccaa 
      caaaaataa ctcaattgc tagcgacaa ccaaatggc 
      gaaaattat gaatctgaa cgcaaatgc tgcacttgc 
      ccggaactg ggaaaatat caacaacgg cacttggaa 
      aatcgtgga atagagcac ttaccttac ataaggtgt 
      gacctagat ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY