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Gene information
General information
TC007382 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5291788..5296813
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007382
    iBeetle screen iB_08120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007382 RA
      MADTAAENE ADSGSGGPA QEAPDGTQE DDAEHNEAE 
RVTGSNTNY DSSEGTHRC TRCDFEAVN KVALAAHVS 
QAHTTNEVK METENNNSP PDRIHRKMF ECDVCNMKF 
SNGANMRRH KMRHTGVKP YECRVCQKR FFRKDHLAE 
HFTTHTKTL PYHCPICNR GFQRQIAMR AHFQNEHVG 
QHDLVKSCP LCSYRAGSM KSLRVHFFN RHGIDLDNP 
GPAGSQASL FAAAAQQGF SMDSALTSL GNLAGSVSV 
SVTGANYSD SGDSTGARS TDNATPPMH FLTPHVEIS 
MADAPAPVF SPNLNDQRS NLPGQSGEV SQRINGECP 
GSPQSADSN SNAPSSSHI QPNSLRIDQ ETNITPSIS 
LIPIKQEPG DELYDVSKR NGPNPSTSP SGSVQMQTA 
TSSSLSSLI KVSPLKSLL RDDLKRKIV SRMNSSPRG 
RSTASPNQN PETSPSDTA TTTNGAITS TGPEQPSDS 
SLWRAMGKR SLSSLQCIF CGIGFPDQT LYFLHKGCH 
SDANPWKCN ICGEQCCNV YEFNSHLLS KSHQ
    CDS sequence
    • >TC007382 RA
      atggccgac actgcagcc gaaaatgag gcagattcg 
ggaagtggt ggccctgca caggaggcc cctgacggc 
actcaagaa gacgacgcc gaacacaac gaggccgag 
cgtgtgacg ggttcaaac acgaactac gattccagc 
gaaggcacc catcgctgc acccgatgc gactttgaa 
gccgttaat aaagtagcc ttagcagcc cacgtgtcc 
caagcccac accacaaac gaagtcaaa atggaaaca 
gaaaacaat aactcacca cccgaccgg atacaccgg 
aagatgttc gaatgtgac gtctgtaac atgaaattc 
agcaacggg gcgaacatg agacgccac aagatgaga 
cacacgggc gtgaagccc tacgagtgc agagtgtgc 
cagaagagg ttcttccgg aaggaccat ctcgccgaa 
cactttacc actcacacg aagacgttg ccttaccat 
tgccccatt tgcaacagg gggttccaa aggcagatt 
gccatgagg gcccatttt cagaatgag catgtgggg 
caacatgac ttggtcaag tcgtgcccg ctttgtagc 
tacagggcg ggctcgatg aagagtctg cgggtgcat 
tttttcaat agacatggg attgatttg gataatcca 
ggcccggct gggtctcag gcgtctctg tttgcggcg 
gccgcccaa cagggcttt tccatggac agtgctttg 
acttcgttg ggtaatttg gcggggagc gtgtctgtg 
agcgtaaca ggggcgaat tatagcgac tctggggat 
tccactggc gctcgttcg acggataac gcaacccca 
cctatgcat tttctaaca ccacacgtg gagatttca 
atggccgat gctccggcg cccgtcttt tccccgaat 
cttaacgac cagcgttcg aatttgccg ggccaaagc 
ggcgaagtg tcccaaagg ataaacggc gagtgtccc 
ggctcccct caatcagcc gactccaat tcgaatgct 
ccgtccagc agccatatt caaccaaat tcccttaga 
atagaccaa gaaacgaac ataacgccc tcaatttcc 
ctcatcccc ataaaacag gaacctgga gacgagctg 
tacgacgtt tccaaacga aacggccct aacccaagc 
acctctccc tcgggaagc gtacaaatg caaacagcg 
acctcaagt agccttagc tcgttaatc aaagtgtct 
ccgttgaag agcttactt cgtgacgat ttaaagagg 
aagatcgtc tcgcggatg aactcctcg ccaaggggg 
cgctccacc gcgagtccc aaccagaat cctgagact 
tcgcccagt gacacggcg accaccacc aatggggct 
atcacctcg acggggccc gaacaaccg tcggactcc 
tccctgtgg cgggcgatg ggcaaaagg tctttgtct 
agtttacaa tgtattttc tgcgggatc ggatttcca 
gaccagact ttgtacttt ttgcataaa gggtgtcac 
tccgacgcg aatccttgg aaatgcaat atctgcgga 
gagcagtgc tgcaatgtt tacgagttt aattcgcat 
ttgttgagt aaaagtcat cagtag
    mRNA sequence
    • >TC007382 RA
      aaaagtgca tcactgttt attaattaa cttgagcaa 
atccttcgc acaaaaacg gtgcaatta gtgataaac 
agcgaagaa aagttgtca ttttaatct gtgcaatta 
tgtcgcaac gcaattaca gcaggtggt tgggaacaa 
tgtagcaca catctctaa cccagggcc gcattccgc 
ccaatgcgt cggcctggt cccggggct gcttccagc 
agccccgtg cgtgaaaca catcgacgg ttcgcgatg 
aatgcgtga taatgtggg tgtttcacg cacgatctg 
ctggtgcta ggtcccgat gacagcgtg gccgcgccg 
ggcgaatcc ggccctggt cggctcaca atcccactt 
cctccgaca ggtcagcct ggaatggcc gaaaagcgg 
tcaaacgtt gtacaggtg ttttttaaa tgtagttta 
tagagtcgg acgttgcga gcatcagtg gcaagtgtt 
gatggaatc cccaagagc gccgtctga gtccttcaa 
gttcctgcg gtgatcatg gccgacact gcagccgaa 
aatgaggca gattcggga agtggtggc cctgcacag 
gaggcccct gacggcact caagaagac gacgccgaa 
cacaacgag gccgagcgt gtgacgggt tcaaacacg 
aactacgat tccagcgaa ggcacccat cgctgcacc 
cgatgcgac tttgaagcc gttaataaa gtagcctta 
gcagcccac gtgtcccaa gcccacacc acaaacgaa 
gtcaaaatg gaaacagaa aacaataac tcaccaccc 
gaccggata caccggaag atgttcgaa tgtgacgtc 
tgtaacatg aaattcagc aacggggcg aacatgaga 
cgccacaag atgagacac acgggcgtg aagccctac 
gagtgcaga gtgtgccag aagaggttc ttccggaag 
gaccatctc gccgaacac tttaccact cacacgaag 
acgttgcct taccattgc cccatttgc aacaggggg 
ttccaaagg cagattgcc atgagggcc cattttcag 
aatgagcat gtggggcaa catgacttg gtcaagtcg 
tgcccgctt tgtagctac agggcgggc tcgatgaag 
agtctgcgg gtgcatttt ttcaataga catgggatt 
gatttggat aatccaggc ccggctggg tctcaggcg 
tctctgttt gcggcggcc gcccaacag ggcttttcc 
atggacagt gctttgact tcgttgggt aatttggcg 
gggagcgtg tctgtgagc gtaacaggg gcgaattat 
agcgactct ggggattcc actggcgct cgttcgacg 
gataacgca accccacct atgcatttt ctaacacca 
cacgtggag atttcaatg gccgatgct ccggcgccc 
gtcttttcc ccgaatctt aacgaccag cgttcgaat 
ttgccgggc caaagcggc gaagtgtcc caaaggata 
aacggcgag tgtcccggc tcccctcaa tcagccgac 
tccaattcg aatgctccg tccagcagc catattcaa 
ccaaattcc cttagaata gaccaagaa acgaacata 
acgccctca atttccctc atccccata aaacaggaa 
cctggagac gagctgtac gacgtttcc aaacgaaac 
ggccctaac ccaagcacc tctccctcg ggaagcgta 
caaatgcaa acagcgacc tcaagtagc cttagctcg 
ttaatcaaa gtgtctccg ttgaagagc ttacttcgt 
gacgattta aagaggaag atcgtctcg cggatgaac 
tcctcgcca agggggcgc tccaccgcg agtcccaac 
cagaatcct gagacttcg cccagtgac acggcgacc 
accaccaat ggggctatc acctcgacg gggcccgaa 
caaccgtcg gactcctcc ctgtggcgg gcgatgggc 
aaaaggtct ttgtctagt ttacaatgt attttctgc 
gggatcgga tttccagac cagactttg tactttttg 
cataaaggg tgtcactcc gacgcgaat ccttggaaa 
tgcaatatc tgcggagag cagtgctgc aatgtttac 
gagtttaat tcgcatttg ttgagtaaa agtcatcag 
taggctcga tttacttaa actatgaca tactttgta 
aagtttaga ttagttcaa gtttcatac agttttaca 
cggaaaaat tgagtatgt gataatttg ttgtagaaa 
ttgttgatg catttcttg tttttgaac aagttcagg 
gttttatgg gtacattta cgagcattc cgtttagtt 
tcattccct tttggaaat tgtccaagt gcctgccgg 
ttgcctata agcaaaatt acctgaaga tttgctcta 
aactcatat ccgtccatt agtaagaag agaggtata 
tttacaagc atgatattt tccaaaata tattgtaaa 
gcaagtgct tagtctcta tagttggag cttcattac 
attgttaca taaattaga attaagttt tacatttta 
tatatacat tccatttta gtgtattgt tgattccat 
gagacagaa acaaaatag tttgtacag tatttattg 
ttatatttt atggtaatt ttatttcgt gcaatatat 
tttgcagtg ccttaaaca aaattgcac tcggacgtt 
taagttcat ttaaatttt tttacgaag taaggattt 
tattttcgt aggcaatta tcgtagtgt agatagtag 
ggcgacatg ttacattcc ttcagttca ttcaaaaat 
atagatata ttttttttt tatttatac acaaaatgt 
aaattacaa attcttgta attctggtg aaagggtta 
aataaactt tatggaatt tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY