| General information | |||
| TC007382 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5291788..5296813 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007382 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08120 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007382 RA
MADTAAENE ADSGSGGPA QEAPDGTQE DDAEHNEAE RVTGSNTNY DSSEGTHRC TRCDFEAVN KVALAAHVS QAHTTNEVK METENNNSP PDRIHRKMF ECDVCNMKF SNGANMRRH KMRHTGVKP YECRVCQKR FFRKDHLAE HFTTHTKTL PYHCPICNR GFQRQIAMR AHFQNEHVG QHDLVKSCP LCSYRAGSM KSLRVHFFN RHGIDLDNP GPAGSQASL FAAAAQQGF SMDSALTSL GNLAGSVSV SVTGANYSD SGDSTGARS TDNATPPMH FLTPHVEIS MADAPAPVF SPNLNDQRS NLPGQSGEV SQRINGECP GSPQSADSN SNAPSSSHI QPNSLRIDQ ETNITPSIS LIPIKQEPG DELYDVSKR NGPNPSTSP SGSVQMQTA TSSSLSSLI KVSPLKSLL RDDLKRKIV SRMNSSPRG RSTASPNQN PETSPSDTA TTTNGAITS TGPEQPSDS SLWRAMGKR SLSSLQCIF CGIGFPDQT LYFLHKGCH SDANPWKCN ICGEQCCNV YEFNSHLLS KSHQ
- >TC007382 RA
atggccgac actgcagcc gaaaatgag gcagattcg ggaagtggt ggccctgca caggaggcc cctgacggc actcaagaa gacgacgcc gaacacaac gaggccgag cgtgtgacg ggttcaaac acgaactac gattccagc gaaggcacc catcgctgc acccgatgc gactttgaa gccgttaat aaagtagcc ttagcagcc cacgtgtcc caagcccac accacaaac gaagtcaaa atggaaaca gaaaacaat aactcacca cccgaccgg atacaccgg aagatgttc gaatgtgac gtctgtaac atgaaattc agcaacggg gcgaacatg agacgccac aagatgaga cacacgggc gtgaagccc tacgagtgc agagtgtgc cagaagagg ttcttccgg aaggaccat ctcgccgaa cactttacc actcacacg aagacgttg ccttaccat tgccccatt tgcaacagg gggttccaa aggcagatt gccatgagg gcccatttt cagaatgag catgtgggg caacatgac ttggtcaag tcgtgcccg ctttgtagc tacagggcg ggctcgatg aagagtctg cgggtgcat tttttcaat agacatggg attgatttg gataatcca ggcccggct gggtctcag gcgtctctg tttgcggcg gccgcccaa cagggcttt tccatggac agtgctttg acttcgttg ggtaatttg gcggggagc gtgtctgtg agcgtaaca ggggcgaat tatagcgac tctggggat tccactggc gctcgttcg acggataac gcaacccca cctatgcat tttctaaca ccacacgtg gagatttca atggccgat gctccggcg cccgtcttt tccccgaat cttaacgac cagcgttcg aatttgccg ggccaaagc ggcgaagtg tcccaaagg ataaacggc gagtgtccc ggctcccct caatcagcc gactccaat tcgaatgct ccgtccagc agccatatt caaccaaat tcccttaga atagaccaa gaaacgaac ataacgccc tcaatttcc ctcatcccc ataaaacag gaacctgga gacgagctg tacgacgtt tccaaacga aacggccct aacccaagc acctctccc tcgggaagc gtacaaatg caaacagcg acctcaagt agccttagc tcgttaatc aaagtgtct ccgttgaag agcttactt cgtgacgat ttaaagagg aagatcgtc tcgcggatg aactcctcg ccaaggggg cgctccacc gcgagtccc aaccagaat cctgagact tcgcccagt gacacggcg accaccacc aatggggct atcacctcg acggggccc gaacaaccg tcggactcc tccctgtgg cgggcgatg ggcaaaagg tctttgtct agtttacaa tgtattttc tgcgggatc ggatttcca gaccagact ttgtacttt ttgcataaa gggtgtcac tccgacgcg aatccttgg aaatgcaat atctgcgga gagcagtgc tgcaatgtt tacgagttt aattcgcat ttgttgagt aaaagtcat cagtag
- >TC007382 RA
aaaagtgca tcactgttt attaattaa cttgagcaa atccttcgc acaaaaacg gtgcaatta gtgataaac agcgaagaa aagttgtca ttttaatct gtgcaatta tgtcgcaac gcaattaca gcaggtggt tgggaacaa tgtagcaca catctctaa cccagggcc gcattccgc ccaatgcgt cggcctggt cccggggct gcttccagc agccccgtg cgtgaaaca catcgacgg ttcgcgatg aatgcgtga taatgtggg tgtttcacg cacgatctg ctggtgcta ggtcccgat gacagcgtg gccgcgccg ggcgaatcc ggccctggt cggctcaca atcccactt cctccgaca ggtcagcct ggaatggcc gaaaagcgg tcaaacgtt gtacaggtg ttttttaaa tgtagttta tagagtcgg acgttgcga gcatcagtg gcaagtgtt gatggaatc cccaagagc gccgtctga gtccttcaa gttcctgcg gtgatcatg gccgacact gcagccgaa aatgaggca gattcggga agtggtggc cctgcacag gaggcccct gacggcact caagaagac gacgccgaa cacaacgag gccgagcgt gtgacgggt tcaaacacg aactacgat tccagcgaa ggcacccat cgctgcacc cgatgcgac tttgaagcc gttaataaa gtagcctta gcagcccac gtgtcccaa gcccacacc acaaacgaa gtcaaaatg gaaacagaa aacaataac tcaccaccc gaccggata caccggaag atgttcgaa tgtgacgtc tgtaacatg aaattcagc aacggggcg aacatgaga cgccacaag atgagacac acgggcgtg aagccctac gagtgcaga gtgtgccag aagaggttc ttccggaag gaccatctc gccgaacac tttaccact cacacgaag acgttgcct taccattgc cccatttgc aacaggggg ttccaaagg cagattgcc atgagggcc cattttcag aatgagcat gtggggcaa catgacttg gtcaagtcg tgcccgctt tgtagctac agggcgggc tcgatgaag agtctgcgg gtgcatttt ttcaataga catgggatt gatttggat aatccaggc ccggctggg tctcaggcg tctctgttt gcggcggcc gcccaacag ggcttttcc atggacagt gctttgact tcgttgggt aatttggcg gggagcgtg tctgtgagc gtaacaggg gcgaattat agcgactct ggggattcc actggcgct cgttcgacg gataacgca accccacct atgcatttt ctaacacca cacgtggag atttcaatg gccgatgct ccggcgccc gtcttttcc ccgaatctt aacgaccag cgttcgaat ttgccgggc caaagcggc gaagtgtcc caaaggata aacggcgag tgtcccggc tcccctcaa tcagccgac tccaattcg aatgctccg tccagcagc catattcaa ccaaattcc cttagaata gaccaagaa acgaacata acgccctca atttccctc atccccata aaacaggaa cctggagac gagctgtac gacgtttcc aaacgaaac ggccctaac ccaagcacc tctccctcg ggaagcgta caaatgcaa acagcgacc tcaagtagc cttagctcg ttaatcaaa gtgtctccg ttgaagagc ttacttcgt gacgattta aagaggaag atcgtctcg cggatgaac tcctcgcca agggggcgc tccaccgcg agtcccaac cagaatcct gagacttcg cccagtgac acggcgacc accaccaat ggggctatc acctcgacg gggcccgaa caaccgtcg gactcctcc ctgtggcgg gcgatgggc aaaaggtct ttgtctagt ttacaatgt attttctgc gggatcgga tttccagac cagactttg tactttttg cataaaggg tgtcactcc gacgcgaat ccttggaaa tgcaatatc tgcggagag cagtgctgc aatgtttac gagtttaat tcgcatttg ttgagtaaa agtcatcag taggctcga tttacttaa actatgaca tactttgta aagtttaga ttagttcaa gtttcatac agttttaca cggaaaaat tgagtatgt gataatttg ttgtagaaa ttgttgatg catttcttg tttttgaac aagttcagg gttttatgg gtacattta cgagcattc cgtttagtt tcattccct tttggaaat tgtccaagt gcctgccgg ttgcctata agcaaaatt acctgaaga tttgctcta aactcatat ccgtccatt agtaagaag agaggtata tttacaagc atgatattt tccaaaata tattgtaaa gcaagtgct tagtctcta tagttggag cttcattac attgttaca taaattaga attaagttt tacatttta tatatacat tccatttta gtgtattgt tgattccat gagacagaa acaaaatag tttgtacag tatttattg ttatatttt atggtaatt ttatttcgt gcaatatat tttgcagtg ccttaaaca aaattgcac tcggacgtt taagttcat ttaaatttt tttacgaag taaggattt tattttcgt aggcaatta tcgtagtgt agatagtag ggcgacatg ttacattcc ttcagttca ttcaaaaat atagatata ttttttttt tatttatac acaaaatgt aaattacaa attcttgta attctggtg aaagggtta aataaactt tatggaatt tt
