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Gene information
General information
TC007382 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5291788..5296813
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007382
    iBeetle screen iB_08120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007382 RA
      MADTAAENE ADSGSGGPA QEAPDGTQE DDAEHNEAE 
      RVTGSNTNY DSSEGTHRC TRCDFEAVN KVALAAHVS 
      QAHTTNEVK METENNNSP PDRIHRKMF ECDVCNMKF 
      SNGANMRRH KMRHTGVKP YECRVCQKR FFRKDHLAE 
      HFTTHTKTL PYHCPICNR GFQRQIAMR AHFQNEHVG 
      QHDLVKSCP LCSYRAGSM KSLRVHFFN RHGIDLDNP 
      GPAGSQASL FAAAAQQGF SMDSALTSL GNLAGSVSV 
      SVTGANYSD SGDSTGARS TDNATPPMH FLTPHVEIS 
      MADAPAPVF SPNLNDQRS NLPGQSGEV SQRINGECP 
      GSPQSADSN SNAPSSSHI QPNSLRIDQ ETNITPSIS 
      LIPIKQEPG DELYDVSKR NGPNPSTSP SGSVQMQTA 
      TSSSLSSLI KVSPLKSLL RDDLKRKIV SRMNSSPRG 
      RSTASPNQN PETSPSDTA TTTNGAITS TGPEQPSDS 
      SLWRAMGKR SLSSLQCIF CGIGFPDQT LYFLHKGCH 
      SDANPWKCN ICGEQCCNV YEFNSHLLS KSHQ
    CDS sequence
    • >TC007382 RA
      atggccgac actgcagcc gaaaatgag gcagattcg 
      ggaagtggt ggccctgca caggaggcc cctgacggc 
      actcaagaa gacgacgcc gaacacaac gaggccgag 
      cgtgtgacg ggttcaaac acgaactac gattccagc 
      gaaggcacc catcgctgc acccgatgc gactttgaa 
      gccgttaat aaagtagcc ttagcagcc cacgtgtcc 
      caagcccac accacaaac gaagtcaaa atggaaaca 
      gaaaacaat aactcacca cccgaccgg atacaccgg 
      aagatgttc gaatgtgac gtctgtaac atgaaattc 
      agcaacggg gcgaacatg agacgccac aagatgaga 
      cacacgggc gtgaagccc tacgagtgc agagtgtgc 
      cagaagagg ttcttccgg aaggaccat ctcgccgaa 
      cactttacc actcacacg aagacgttg ccttaccat 
      tgccccatt tgcaacagg gggttccaa aggcagatt 
      gccatgagg gcccatttt cagaatgag catgtgggg 
      caacatgac ttggtcaag tcgtgcccg ctttgtagc 
      tacagggcg ggctcgatg aagagtctg cgggtgcat 
      tttttcaat agacatggg attgatttg gataatcca 
      ggcccggct gggtctcag gcgtctctg tttgcggcg 
      gccgcccaa cagggcttt tccatggac agtgctttg 
      acttcgttg ggtaatttg gcggggagc gtgtctgtg 
      agcgtaaca ggggcgaat tatagcgac tctggggat 
      tccactggc gctcgttcg acggataac gcaacccca 
      cctatgcat tttctaaca ccacacgtg gagatttca 
      atggccgat gctccggcg cccgtcttt tccccgaat 
      cttaacgac cagcgttcg aatttgccg ggccaaagc 
      ggcgaagtg tcccaaagg ataaacggc gagtgtccc 
      ggctcccct caatcagcc gactccaat tcgaatgct 
      ccgtccagc agccatatt caaccaaat tcccttaga 
      atagaccaa gaaacgaac ataacgccc tcaatttcc 
      ctcatcccc ataaaacag gaacctgga gacgagctg 
      tacgacgtt tccaaacga aacggccct aacccaagc 
      acctctccc tcgggaagc gtacaaatg caaacagcg 
      acctcaagt agccttagc tcgttaatc aaagtgtct 
      ccgttgaag agcttactt cgtgacgat ttaaagagg 
      aagatcgtc tcgcggatg aactcctcg ccaaggggg 
      cgctccacc gcgagtccc aaccagaat cctgagact 
      tcgcccagt gacacggcg accaccacc aatggggct 
      atcacctcg acggggccc gaacaaccg tcggactcc 
      tccctgtgg cgggcgatg ggcaaaagg tctttgtct 
      agtttacaa tgtattttc tgcgggatc ggatttcca 
      gaccagact ttgtacttt ttgcataaa gggtgtcac 
      tccgacgcg aatccttgg aaatgcaat atctgcgga 
      gagcagtgc tgcaatgtt tacgagttt aattcgcat 
      ttgttgagt aaaagtcat cagtag
    mRNA sequence
    • >TC007382 RA
      aaaagtgca tcactgttt attaattaa cttgagcaa 
      atccttcgc acaaaaacg gtgcaatta gtgataaac 
      agcgaagaa aagttgtca ttttaatct gtgcaatta 
      tgtcgcaac gcaattaca gcaggtggt tgggaacaa 
      tgtagcaca catctctaa cccagggcc gcattccgc 
      ccaatgcgt cggcctggt cccggggct gcttccagc 
      agccccgtg cgtgaaaca catcgacgg ttcgcgatg 
      aatgcgtga taatgtggg tgtttcacg cacgatctg 
      ctggtgcta ggtcccgat gacagcgtg gccgcgccg 
      ggcgaatcc ggccctggt cggctcaca atcccactt 
      cctccgaca ggtcagcct ggaatggcc gaaaagcgg 
      tcaaacgtt gtacaggtg ttttttaaa tgtagttta 
      tagagtcgg acgttgcga gcatcagtg gcaagtgtt 
      gatggaatc cccaagagc gccgtctga gtccttcaa 
      gttcctgcg gtgatcatg gccgacact gcagccgaa 
      aatgaggca gattcggga agtggtggc cctgcacag 
      gaggcccct gacggcact caagaagac gacgccgaa 
      cacaacgag gccgagcgt gtgacgggt tcaaacacg 
      aactacgat tccagcgaa ggcacccat cgctgcacc 
      cgatgcgac tttgaagcc gttaataaa gtagcctta 
      gcagcccac gtgtcccaa gcccacacc acaaacgaa 
      gtcaaaatg gaaacagaa aacaataac tcaccaccc 
      gaccggata caccggaag atgttcgaa tgtgacgtc 
      tgtaacatg aaattcagc aacggggcg aacatgaga 
      cgccacaag atgagacac acgggcgtg aagccctac 
      gagtgcaga gtgtgccag aagaggttc ttccggaag 
      gaccatctc gccgaacac tttaccact cacacgaag 
      acgttgcct taccattgc cccatttgc aacaggggg 
      ttccaaagg cagattgcc atgagggcc cattttcag 
      aatgagcat gtggggcaa catgacttg gtcaagtcg 
      tgcccgctt tgtagctac agggcgggc tcgatgaag 
      agtctgcgg gtgcatttt ttcaataga catgggatt 
      gatttggat aatccaggc ccggctggg tctcaggcg 
      tctctgttt gcggcggcc gcccaacag ggcttttcc 
      atggacagt gctttgact tcgttgggt aatttggcg 
      gggagcgtg tctgtgagc gtaacaggg gcgaattat 
      agcgactct ggggattcc actggcgct cgttcgacg 
      gataacgca accccacct atgcatttt ctaacacca 
      cacgtggag atttcaatg gccgatgct ccggcgccc 
      gtcttttcc ccgaatctt aacgaccag cgttcgaat 
      ttgccgggc caaagcggc gaagtgtcc caaaggata 
      aacggcgag tgtcccggc tcccctcaa tcagccgac 
      tccaattcg aatgctccg tccagcagc catattcaa 
      ccaaattcc cttagaata gaccaagaa acgaacata 
      acgccctca atttccctc atccccata aaacaggaa 
      cctggagac gagctgtac gacgtttcc aaacgaaac 
      ggccctaac ccaagcacc tctccctcg ggaagcgta 
      caaatgcaa acagcgacc tcaagtagc cttagctcg 
      ttaatcaaa gtgtctccg ttgaagagc ttacttcgt 
      gacgattta aagaggaag atcgtctcg cggatgaac 
      tcctcgcca agggggcgc tccaccgcg agtcccaac 
      cagaatcct gagacttcg cccagtgac acggcgacc 
      accaccaat ggggctatc acctcgacg gggcccgaa 
      caaccgtcg gactcctcc ctgtggcgg gcgatgggc 
      aaaaggtct ttgtctagt ttacaatgt attttctgc 
      gggatcgga tttccagac cagactttg tactttttg 
      cataaaggg tgtcactcc gacgcgaat ccttggaaa 
      tgcaatatc tgcggagag cagtgctgc aatgtttac 
      gagtttaat tcgcatttg ttgagtaaa agtcatcag 
      taggctcga tttacttaa actatgaca tactttgta 
      aagtttaga ttagttcaa gtttcatac agttttaca 
      cggaaaaat tgagtatgt gataatttg ttgtagaaa 
      ttgttgatg catttcttg tttttgaac aagttcagg 
      gttttatgg gtacattta cgagcattc cgtttagtt 
      tcattccct tttggaaat tgtccaagt gcctgccgg 
      ttgcctata agcaaaatt acctgaaga tttgctcta 
      aactcatat ccgtccatt agtaagaag agaggtata 
      tttacaagc atgatattt tccaaaata tattgtaaa 
      gcaagtgct tagtctcta tagttggag cttcattac 
      attgttaca taaattaga attaagttt tacatttta 
      tatatacat tccatttta gtgtattgt tgattccat 
      gagacagaa acaaaatag tttgtacag tatttattg 
      ttatatttt atggtaatt ttatttcgt gcaatatat 
      tttgcagtg ccttaaaca aaattgcac tcggacgtt 
      taagttcat ttaaatttt tttacgaag taaggattt 
      tattttcgt aggcaatta tcgtagtgt agatagtag 
      ggcgacatg ttacattcc ttcagttca ttcaaaaat 
      atagatata ttttttttt tatttatac acaaaatgt 
      aaattacaa attcttgta attctggtg aaagggtta 
      aataaactt tatggaatt tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY