Status
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Gene information
General information
TC004682 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 188686..219510
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004682
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08119

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004682 RA
      MCWFVQQFW NNQKKLDMS RRKQANPAK LDPQEEDDL 
      EAQHLRLAE DETAASSAS GGSGCGETQ PFDDEQVQG 
      DQDQDTKPG PYKCTVCGE NYLKEKLLR EHEQSHTDH 
      LQFTCAYCP RLFKHKRSR DRHTKLHTG DKKYKCQQC 
      DSAFSRSDH LKIHMKTHD SRKPYKCGT CNRGYNTAA 
      ALSSHQQSH LKQESRSGS RTSGGSTPS PGLFRCTHC 
      AETFGKPDL LQSHVAMVH SDTDSSLSQ TPEPLSEYQ 
      NQIEDLKIV CMYCSKEFP SLELMYQHT NIAHRDVPN 
      GVTTSVSVS SPAIQNSPK PEICQNGTP TYACDRCTM 
      QLDSLQNLK NHINNVHWR PALSPNPVN FMGIEKSYS 
      PFQTQPTDL SRKKKSEDS AEKAVKKKK DQHSPTAIS 
      PYDSNDKPC ICSCCYAQL PNFKSFLHH MESHVISSN 
      NSLLGFCPV CGEPGRDPV SFTNHIFSH AIAQVPGRC 
      CYTCKKSFE RLEELQKHL LEVHVVSVF KCSICNDIF 
      DTKISMQLH LTNKHSDEC KHFKCYLCT NQVFHDRLS 
      AELHISMKH YQQFTPCVG ANQLLRSQY QELDTRFRD 
      YNMIFQCTF CHKTFKDQY SQYIHILKE HNESKEGEK 
      FILDSAMNP NPSRSPNFM FPKIVTPDH VEPLESVYT 
      CDICNRSDI TSESDLINH KKLHHSSKN KIGPVSLQC 
      AYCNEYCKS RTDLENHMK THQVTCGKG KHKCNICDE 
      IYSSTLTLA DHKLTHCKI VEGNSCVQC KSVLTDENS 
      FYSHQLQHS NPGKPNSQI SLPANCIIC CQTLQTDVE 
      IKLHAKFHL KHLTQKEYM CGVCSKVFD SQGQPANDV 
      NIVVCKDCA KSEDEGKKV PKQFACIQC PQTFDSESD 
      VQNHAAMHM LNEGTNLEC RLCKQVFSS PLKLQTHLI 
      EHNFYGMNQ YSCYVCSSV FTAASGLQS HIIGHGLDS 
      RPYECSQCQ MKFFFRAEL DNHRFVHLQ KSYPIHNGG 
      EIYENKPSP RYKICKYCS NSYLDNSFF VEHLNQCPF 
      RISPKRETF DNEVDNNIK QEVTEIASE EKDEQ
    • >TC004682 RB
      MCWFVQQFW NNQKKLDMS RRKQANPAK LDPQEEDDL 
      EAQHLRLAE DETAASSAS GGSGCGETQ PFDDEQVQG 
      DQDQDTKPG PYKCTSHTD HLQFTCAYC PRLFKHKRS 
      RDRHTKLHT GDKKYKCQQ CDSAFSRSD HLKIHMKTH 
      DSRKPYKCG TCNRGYNTA AALSSHQQS HLKQESRSG 
      SRTSGGSTP SPGLFRCTH CAETFGKPD LLQSHVAMV 
      HSDTDSSLS QTPEPLSEY QNQIEDLKI VCMYCSKEF 
      PSLELMYQH TNIAHRDVP NGVTTSVSV SSPAIQNSP 
      KPEICQNGT PTYACDRCT MQLDSLQNL KNHINNVHW 
      RPALSPNPV NFMGIEKSY SPFQTQPTD LSRKKKSED 
      SAEKAVKKK KDQHSPTAI SPYDSNDKP CICSCCYAQ 
      LPNFKSFLH HMESHVISS NNSLLGFCP VCGEPGRDP 
      VSFTNHIFS HAIAQVPGR CCYTCKKSF ERLEELQKH 
      LLEVHVVSV FKCSICNDI FDTKISMQL HLTNKHSDE 
      CKHFKCYLC TNQVFHDRL SAELHISMK HYQQFTPCV 
      GANQLLRSQ YQELDTRFR DYNMIFQCT FCHKTFKDQ 
      YSQYIHILK EHNESKEGE KFILDSAMN PNPSRSPNF 
      MFPKIVTPD HVEPLESVY TCDICNRSD ITSESDLIN 
      HKKLHHSSK NKIGPVSLQ CAYCNEYCK SRTDLENHM 
      KTHQVTCGK GKHKCNICD EIYSSTLTL ADHKLTHCK 
      IVEGNSCVQ CKSVLTDEN SFYSHQLQH SNPGKPNSQ 
      ISLPANCII CCQTLQTDV EIKLHAKFH LKHLTQKEY 
      MCGVCSKVF DSQGQPAND VNIVVCKDC AKSEDEGKK 
      VPKQFACIQ CPQTFDSES DVQNHAAMH MLNEGTNLE 
      CRLCKQVFS SPLKLQTHL IEHNFYGMN QYSCYVCSS 
      VFTAASGLQ SHIIGHGLD SRPYECSQC QMKFFFRAE 
      LDNHRFVHL QKSYPIHNG GEIYENKPS PRYKICKYC 
      SNSYLDNSF FVEHLNQCP FRISPKRET FDNEVDNNI 
      KQEVTEIAS EEKDEQ
    • >TC004682 RC
      MCWFVQQFW NNQKKLDMS RRKQANPAK LDPQEEDDL 
      EAQHLRLSD DQFAKMKML FKGNSSRLE LLIEKIQST 
      KEGEFKVPV AEDETAASS ASGGSGCGE TQPFDDEQV 
      QGDQDQDTK PGPYKCTVC GENYLKEKL LREHEQSHT 
      DHLQFTCAY CPRLFKHKR SRDRHTKLH TGDKKYKCQ 
      QCDSAFSRS DHLKIHMKT HDSRKPYKC GTCNRGYNT 
      AAALSSHQQ SHLKQESRS GSRTSGGST PSPGLFRCT 
      HCAETFGKP DLLQSHVAM VHSDTDSSL SQTPEPLSE 
      YQNQIEDLK IVCMYCSKE FPSLELMYQ HTNIAHRDV 
      PNGVTTSVS VSSPAIQNS PKPEICQNG TPTYACDRC 
      TMQLDSLQN LKNHINNVH WRPALSPNP VNFMGIEKS 
      YSPFQTQPT DLSRKKKSE DSAEKAVKK KKDQHSPTA 
      ISPYDSNDK PCICSCCYA QLPNFKSFL HHMESHVIS 
      SNNSLLGFC PVCGEPGRD PVSFTNHIF SHAIAQVPG 
      RCCYTCKKS FERLEELQK HLLEVHVVS VFKCSICND 
      IFDTKISMQ LHLTNKHSD ECKHFKCYL CTNQVFHDR 
      LSAELHISM KHYQQFTPC VGANQLLRS QYQELDTRF 
      RDYNMIFQC TFCHKTFKD QYSQYIHIL KEHNESKEG 
      EKFILDSAM NPNPSRSPN FMFPKIVTP DHVEPLESV 
      YTCDICNRS DITSESDLI NHKKLHHSS KNKIGPVSL 
      QCAYCNEYC KSRTDLENH MKTHQVTCG KGKHKCNIC 
      DEIYSSTLT LADHKLTHC KIVEGNSCV QCKSVLTDE 
      NSFYSHQLQ HSNPGKPNS QISLPANCI ICCQTLQTD 
      VEIKLHAKF HLKHLTQKE YMCGVCSKV FDSQGQPAN 
      DVNIVVCKD CAKSEDEGK KVPKQFACI QCPQTFDSE 
      SDVQNHAAM HMLNEGTNL ECRLCKQVF SSPLKLQTH 
      LIEHNFYGM NQYSCYVCS SVFTAASGL QSHIIGHGL 
      DSRPYECSQ CQMKFFFRA ELDNHRFVH LQKSYPIHN 
      GGEIYENKP SPRYKICKY CSNSYLDNS FFVEHLNQC 
      PFRISPKRE TFDNEVDNN IKQEVTEIA SEEKDEQ
    CDS sequence
    • >TC004682 RA
      atgtgttgg tttgtgcaa cagttttgg aataatcaa 
      aagaagtta gatatgtcc agacggaag caagccaat 
      cctgccaaa ttagatcct caggaggag gacgaccta 
      gaagcacag cacttgaga ctagccgaa gatgaaacg 
      gctgccagt tccgcatcc ggtggttct ggatgtggc 
      gaaactcag ccttttgac gatgaacaa gtgcaggga 
      gatcaagat caagatacc aaacccgga ccatacaaa 
      tgcacggta tgtggagaa aattattta aaagaaaaa 
      ctgctcagg gagcacgaa cagagtcac actgaccat 
      ctgcaattc acttgtgct tattgtcct cggcttttc 
      aagcacaag cgatcgaga gatcgccac acgaagctc 
      cacactggg gataaaaaa tataaatgt cagcagtgc 
      gattccgcc ttttcacga agtgatcat cttaaaatt 
      catatgaaa acacatgat agtcgtaaa ccatataaa 
      tgtggtaca tgcaatagg ggctataac accgccgca 
      gctctctcg tcgcatcaa caaagtcac ttgaaacaa 
      gaatctagg tccggaagt cgcacgagt ggaggaagc 
      actcccagc cccggactt ttccggtgc actcattgt 
      gccgaaact ttcggaaaa ccggatctg ctacagagt 
      catgtcgca atggtccac agcgacacg gactcgtcg 
      ctaagccaa actccggag cccttatca gagtatcaa 
      aaccaaatc gaagacctg aaaatcgtt tgcatgtac 
      tgctcgaag gagttcccc agtttggag ctgatgtac 
      cagcacaca aacatcgcc catcgtgac gtccccaac 
      ggcgttaca acttccgtt tccgtctcc tcccccgcg 
      atccaaaac tccccaaaa ccggaaatt tgccaaaac 
      ggaacacca acctacgcc tgtgataga tgcacgatg 
      caattagac tcgttgcag aacttgaaa aaccacata 
      aacaacgta cactggcgt ccggccctc tcgccaaac 
      cccgtcaat ttcatgggg attgagaaa agctactcc 
      ccgttccag acccaaccg accgacctg agccgcaag 
      aagaaaagc gaggacagt gcggagaaa gcggtcaag 
      aagaagaaa gaccagcac tcccccact gccatcagt 
      ccctacgac tccaacgac aagccctgc atttgctcg 
      tgttgctac gcccaactc ccaaacttc aaaagcttc 
      ctccaccac atggagtcg cacgtaatc tcctcgaac 
      aactccctc ctcggcttc tgccccgtc tgcggcgag 
      cctggccgg gaccccgtc tcgttcacc aaccacatt 
      tttagccac gctattgct caagtacct ggccggtgt 
      tgctacact tgcaagaag tccttcgaa aggctggaa 
      gagctgcag aagcacctg ttggaagtc cacgtcgtg 
      tcggtgttc aagtgttcc atctgcaac gatattttc 
      gatacgaag atttcaatg cagttgcat ctgacgaac 
      aaacacagc gacgaatgc aagcacttc aagtgctac 
      ttgtgcacg aatcaggtg tttcacgat cgactttcg 
      gcagaattg cacatctcc atgaagcac taccagcag 
      ttcacgccc tgtgttggt gccaaccaa ctgctacgg 
      tcgcaatac caggagttg gacaccagg tttcgcgac 
      tataacatg attttccag tgcacgttc tgccacaaa 
      accttcaag gaccagtac tcgcagtat atccacatt 
      ttgaaggag cacaacgaa agcaaagag ggtgaaaaa 
      ttcattttg gattcagca atgaaccca aatccgtcc 
      agatcgccc aacttcatg ttccccaaa atcgtgacc 
      cctgaccac gtggagccc ctggaatcg gtctacact 
      tgtgatata tgcaaccgc agtgatata acctccgaa 
      tcggaccta ataaaccac aagaagctc caccacagc 
      agcaagaac aagatcggt cccgtgagc ctccaatgc 
      gcctactgc aacgagtac tgcaagtcg cgcacggac 
      ctggagaac cacatgaag acccaccaa gtgacctgc 
      ggcaaaggc aagcacaag tgcaacatc tgcgacgag 
      atttactcc tccacgttg accctcgcc gaccacaag 
      ctcacccac tgcaagatc gtcgagggg aacagttgc 
      gtccagtgc aagtcggtg ctaaccgac gagaactcc 
      ttctacagc caccagctg cagcacagc aacccgggg 
      aagcccaac tcgcagatc tcgctccca gcgaactgc 
      atcatctgc tgccagacg ctgcagacg gacgtggag 
      atcaagctc cacgccaag ttccacctg aagcacctg 
      acgcagaag gagtacatg tgcggggtc tgcagcaaa 
      gtgttcgac agccagggg cagcccgcc aacgacgtc 
      aacatcgtc gtgtgcaaa gactgcgcg aagagtgag 
      gacgagggg aaaaaagtg ccgaaacag ttcgcgtgt 
      atccagtgc ccccagact ttcgacagt gaaagtgac 
      gtgcaaaac cacgctgcc atgcacatg ctgaacgag 
      gggacgaac ctggagtgc cgcttgtgc aagcaggtc 
      ttctcgtcg cccctcaag ctgcagacg cacctaatc 
      gagcacaac ttctacgga atgaaccag tacagttgt 
      tacgtgtgt tcctcggtt ttcacggca gctagcggc 
      ctgcaaagc cacataatc gggcatggg ctggacagc 
      agaccctat gagtgttcg caatgccag atgaagttc 
      ttcttccgg gccgagttg gataatcat aggtttgtg 
      cacctccag aagagctat ccgattcac aacgggggc 
      gagatctac gagaacaag ccaagcccc cggtataaa 
      atctgtaaa tactgttcg aactcgtat ctggacaac 
      tcgtttttc gtagaacat ttgaatcag tgtcccttc 
      aggataagt ccgaaacgt gagactttc gataatgaa 
      gtagacaat aatattaaa caagaagta acagagatc 
      gcgtcggag gaaaaagat gagcaataa
    • >TC004682 RB
      atgtgttgg tttgtgcaa cagttttgg aataatcaa 
      aagaagtta gatatgtcc agacggaag caagccaat 
      cctgccaaa ttagatcct caggaggag gacgaccta 
      gaagcacag cacttgaga ctagccgaa gatgaaacg 
      gctgccagt tccgcatcc ggtggttct ggatgtggc 
      gaaactcag ccttttgac gatgaacaa gtgcaggga 
      gatcaagat caagatacc aaacccgga ccatacaaa 
      tgcacgagt cacactgac catctgcaa ttcacttgt 
      gcttattgt cctcggctt ttcaagcac aagcgatcg 
      agagatcgc cacacgaag ctccacact ggggataaa 
      aaatataaa tgtcagcag tgcgattcc gccttttca 
      cgaagtgat catcttaaa attcatatg aaaacacat 
      gatagtcgt aaaccatat aaatgtggt acatgcaat 
      aggggctat aacaccgcc gcagctctc tcgtcgcat 
      caacaaagt cacttgaaa caagaatct aggtccgga 
      agtcgcacg agtggagga agcactccc agccccgga 
      cttttccgg tgcactcat tgtgccgaa actttcgga 
      aaaccggat ctgctacag agtcatgtc gcaatggtc 
      cacagcgac acggactcg tcgctaagc caaactccg 
      gagccctta tcagagtat caaaaccaa atcgaagac 
      ctgaaaatc gtttgcatg tactgctcg aaggagttc 
      cccagtttg gagctgatg taccagcac acaaacatc 
      gcccatcgt gacgtcccc aacggcgtt acaacttcc 
      gtttccgtc tcctccccc gcgatccaa aactcccca 
      aaaccggaa atttgccaa aacggaaca ccaacctac 
      gcctgtgat agatgcacg atgcaatta gactcgttg 
      cagaacttg aaaaaccac ataaacaac gtacactgg 
      cgtccggcc ctctcgcca aaccccgtc aatttcatg 
      gggattgag aaaagctac tccccgttc cagacccaa 
      ccgaccgac ctgagccgc aagaagaaa agcgaggac 
      agtgcggag aaagcggtc aagaagaag aaagaccag 
      cactccccc actgccatc agtccctac gactccaac 
      gacaagccc tgcatttgc tcgtgttgc tacgcccaa 
      ctcccaaac ttcaaaagc ttcctccac cacatggag 
      tcgcacgta atctcctcg aacaactcc ctcctcggc 
      ttctgcccc gtctgcggc gagcctggc cgggacccc 
      gtctcgttc accaaccac atttttagc cacgctatt 
      gctcaagta cctggccgg tgttgctac acttgcaag 
      aagtccttc gaaaggctg gaagagctg cagaagcac 
      ctgttggaa gtccacgtc gtgtcggtg ttcaagtgt 
      tccatctgc aacgatatt ttcgatacg aagatttca 
      atgcagttg catctgacg aacaaacac agcgacgaa 
      tgcaagcac ttcaagtgc tacttgtgc acgaatcag 
      gtgtttcac gatcgactt tcggcagaa ttgcacatc 
      tccatgaag cactaccag cagttcacg ccctgtgtt 
      ggtgccaac caactgcta cggtcgcaa taccaggag 
      ttggacacc aggtttcgc gactataac atgattttc 
      cagtgcacg ttctgccac aaaaccttc aaggaccag 
      tactcgcag tatatccac attttgaag gagcacaac 
      gaaagcaaa gagggtgaa aaattcatt ttggattca 
      gcaatgaac ccaaatccg tccagatcg cccaacttc 
      atgttcccc aaaatcgtg acccctgac cacgtggag 
      cccctggaa tcggtctac acttgtgat atatgcaac 
      cgcagtgat ataacctcc gaatcggac ctaataaac 
      cacaagaag ctccaccac agcagcaag aacaagatc 
      ggtcccgtg agcctccaa tgcgcctac tgcaacgag 
      tactgcaag tcgcgcacg gacctggag aaccacatg 
      aagacccac caagtgacc tgcggcaaa ggcaagcac 
      aagtgcaac atctgcgac gagatttac tcctccacg 
      ttgaccctc gccgaccac aagctcacc cactgcaag 
      atcgtcgag gggaacagt tgcgtccag tgcaagtcg 
      gtgctaacc gacgagaac tccttctac agccaccag 
      ctgcagcac agcaacccg gggaagccc aactcgcag 
      atctcgctc ccagcgaac tgcatcatc tgctgccag 
      acgctgcag acggacgtg gagatcaag ctccacgcc 
      aagttccac ctgaagcac ctgacgcag aaggagtac 
      atgtgcggg gtctgcagc aaagtgttc gacagccag 
      gggcagccc gccaacgac gtcaacatc gtcgtgtgc 
      aaagactgc gcgaagagt gaggacgag gggaaaaaa 
      gtgccgaaa cagttcgcg tgtatccag tgcccccag 
      actttcgac agtgaaagt gacgtgcaa aaccacgct 
      gccatgcac atgctgaac gaggggacg aacctggag 
      tgccgcttg tgcaagcag gtcttctcg tcgcccctc 
      aagctgcag acgcaccta atcgagcac aacttctac 
      ggaatgaac cagtacagt tgttacgtg tgttcctcg 
      gttttcacg gcagctagc ggcctgcaa agccacata 
      atcgggcat gggctggac agcagaccc tatgagtgt 
      tcgcaatgc cagatgaag ttcttcttc cgggccgag 
      ttggataat cataggttt gtgcacctc cagaagagc 
      tatccgatt cacaacggg ggcgagatc tacgagaac 
      aagccaagc ccccggtat aaaatctgt aaatactgt 
      tcgaactcg tatctggac aactcgttt ttcgtagaa 
      catttgaat cagtgtccc ttcaggata agtccgaaa 
      cgtgagact ttcgataat gaagtagac aataatatt 
      aaacaagaa gtaacagag atcgcgtcg gaggaaaaa 
      gatgagcaa taa
    • >TC004682 RC
      atgtgttgg tttgtgcaa cagttttgg aataatcaa 
      aagaagtta gatatgtcc agacggaag caagccaat 
      cctgccaaa ttagatcct caggaggag gacgaccta 
      gaagcacag cacttgaga ctgagtgac gaccagttc 
      gccaaaatg aaaatgcta ttcaaggga aatagcagc 
      cgcttggag ctactaatc gaaaaaatt cagtccacc 
      aaagagggc gaatttaaa gtgccagta gccgaagat 
      gaaacggct gccagttcc gcatccggt ggttctgga 
      tgtggcgaa actcagcct tttgacgat gaacaagtg 
      cagggagat caagatcaa gataccaaa cccggacca 
      tacaaatgc acggtatgt ggagaaaat tatttaaaa 
      gaaaaactg ctcagggag cacgaacag agtcacact 
      gaccatctg caattcact tgtgcttat tgtcctcgg 
      cttttcaag cacaagcga tcgagagat cgccacacg 
      aagctccac actggggat aaaaaatat aaatgtcag 
      cagtgcgat tccgccttt tcacgaagt gatcatctt 
      aaaattcat atgaaaaca catgatagt cgtaaacca 
      tataaatgt ggtacatgc aataggggc tataacacc 
      gccgcagct ctctcgtcg catcaacaa agtcacttg 
      aaacaagaa tctaggtcc ggaagtcgc acgagtgga 
      ggaagcact cccagcccc ggacttttc cggtgcact 
      cattgtgcc gaaactttc ggaaaaccg gatctgcta 
      cagagtcat gtcgcaatg gtccacagc gacacggac 
      tcgtcgcta agccaaact ccggagccc ttatcagag 
      tatcaaaac caaatcgaa gacctgaaa atcgtttgc 
      atgtactgc tcgaaggag ttccccagt ttggagctg 
      atgtaccag cacacaaac atcgcccat cgtgacgtc 
      cccaacggc gttacaact tccgtttcc gtctcctcc 
      cccgcgatc caaaactcc ccaaaaccg gaaatttgc 
      caaaacgga acaccaacc tacgcctgt gatagatgc 
      acgatgcaa ttagactcg ttgcagaac ttgaaaaac 
      cacataaac aacgtacac tggcgtccg gccctctcg 
      ccaaacccc gtcaatttc atggggatt gagaaaagc 
      tactccccg ttccagacc caaccgacc gacctgagc 
      cgcaagaag aaaagcgag gacagtgcg gagaaagcg 
      gtcaagaag aagaaagac cagcactcc cccactgcc 
      atcagtccc tacgactcc aacgacaag ccctgcatt 
      tgctcgtgt tgctacgcc caactccca aacttcaaa 
      agcttcctc caccacatg gagtcgcac gtaatctcc 
      tcgaacaac tccctcctc ggcttctgc cccgtctgc 
      ggcgagcct ggccgggac cccgtctcg ttcaccaac 
      cacattttt agccacgct attgctcaa gtacctggc 
      cggtgttgc tacacttgc aagaagtcc ttcgaaagg 
      ctggaagag ctgcagaag cacctgttg gaagtccac 
      gtcgtgtcg gtgttcaag tgttccatc tgcaacgat 
      attttcgat acgaagatt tcaatgcag ttgcatctg 
      acgaacaaa cacagcgac gaatgcaag cacttcaag 
      tgctacttg tgcacgaat caggtgttt cacgatcga 
      ctttcggca gaattgcac atctccatg aagcactac 
      cagcagttc acgccctgt gttggtgcc aaccaactg 
      ctacggtcg caataccag gagttggac accaggttt 
      cgcgactat aacatgatt ttccagtgc acgttctgc 
      cacaaaacc ttcaaggac cagtactcg cagtatatc 
      cacattttg aaggagcac aacgaaagc aaagagggt 
      gaaaaattc attttggat tcagcaatg aacccaaat 
      ccgtccaga tcgcccaac ttcatgttc cccaaaatc 
      gtgacccct gaccacgtg gagcccctg gaatcggtc 
      tacacttgt gatatatgc aaccgcagt gatataacc 
      tccgaatcg gacctaata aaccacaag aagctccac 
      cacagcagc aagaacaag atcggtccc gtgagcctc 
      caatgcgcc tactgcaac gagtactgc aagtcgcgc 
      acggacctg gagaaccac atgaagacc caccaagtg 
      acctgcggc aaaggcaag cacaagtgc aacatctgc 
      gacgagatt tactcctcc acgttgacc ctcgccgac 
      cacaagctc acccactgc aagatcgtc gaggggaac 
      agttgcgtc cagtgcaag tcggtgcta accgacgag 
      aactccttc tacagccac cagctgcag cacagcaac 
      ccggggaag cccaactcg cagatctcg ctcccagcg 
      aactgcatc atctgctgc cagacgctg cagacggac 
      gtggagatc aagctccac gccaagttc cacctgaag 
      cacctgacg cagaaggag tacatgtgc ggggtctgc 
      agcaaagtg ttcgacagc caggggcag cccgccaac 
      gacgtcaac atcgtcgtg tgcaaagac tgcgcgaag 
      agtgaggac gaggggaaa aaagtgccg aaacagttc 
      gcgtgtatc cagtgcccc cagactttc gacagtgaa 
      agtgacgtg caaaaccac gctgccatg cacatgctg 
      aacgagggg acgaacctg gagtgccgc ttgtgcaag 
      caggtcttc tcgtcgccc ctcaagctg cagacgcac 
      ctaatcgag cacaacttc tacggaatg aaccagtac 
      agttgttac gtgtgttcc tcggttttc acggcagct 
      agcggcctg caaagccac ataatcggg catgggctg 
      gacagcaga ccctatgag tgttcgcaa tgccagatg 
      aagttcttc ttccgggcc gagttggat aatcatagg 
      tttgtgcac ctccagaag agctatccg attcacaac 
      gggggcgag atctacgag aacaagcca agcccccgg 
      tataaaatc tgtaaatac tgttcgaac tcgtatctg 
      gacaactcg tttttcgta gaacatttg aatcagtgt 
      cccttcagg ataagtccg aaacgtgag actttcgat 
      aatgaagta gacaataat attaaacaa gaagtaaca 
      gagatcgcg tcggaggaa aaagatgag caataa
    mRNA sequence
    • >TC004682 RA
      cagtgcgtg acaacttaa aacggagcg ataaatggg 
      gtcatgatt acaccaaac accgcatcc aaaagtgaa 
      acgctcaaa gttgattgc aaaaacggg ataaatgtg 
      ttggtttgt gcaacagtt ttggaataa tcaaaagaa 
      gttagatat gtccagacg gaagcaagc caatcctgc 
      caaattaga tcctcagga ggaggacga cctagaagc 
      acagcactt gagactagc cgaagatga aacggctgc 
      cagttccgc atccggtgg ttctggatg tggcgaaac 
      tcagccttt tgacgatga acaagtgca gggagatca 
      agatcaaga taccaaacc cggaccata caaatgcac 
      ggtatgtgg agaaaatta tttaaaaga aaaactgct 
      cagggagca cgaacagag tcacactga ccatctgca 
      attcacttg tgcttattg tcctcggct tttcaagca 
      caagcgatc gagagatcg ccacacgaa gctccacac 
      tggggataa aaaatataa atgtcagca gtgcgattc 
      cgccttttc acgaagtga tcatcttaa aattcatat 
      gaaaacaca tgatagtcg taaaccata taaatgtgg 
      tacatgcaa taggggcta taacaccgc cgcagctct 
      ctcgtcgca tcaacaaag tcacttgaa acaagaatc 
      taggtccgg aagtcgcac gagtggagg aagcactcc 
      cagccccgg acttttccg gtgcactca ttgtgccga 
      aactttcgg aaaaccgga tctgctaca gagtcatgt 
      cgcaatggt ccacagcga cacggactc gtcgctaag 
      ccaaactcc ggagccctt atcagagta tcaaaacca 
      aatcgaaga cctgaaaat cgtttgcat gtactgctc 
      gaaggagtt ccccagttt ggagctgat gtaccagca 
      cacaaacat cgcccatcg tgacgtccc caacggcgt 
      tacaacttc cgtttccgt ctcctcccc cgcgatcca 
      aaactcccc aaaaccgga aatttgcca aaacggaac 
      accaaccta cgcctgtga tagatgcac gatgcaatt 
      agactcgtt gcagaactt gaaaaacca cataaacaa 
      cgtacactg gcgtccggc cctctcgcc aaaccccgt 
      caatttcat ggggattga gaaaagcta ctccccgtt 
      ccagaccca accgaccga cctgagccg caagaagaa 
      aagcgagga cagtgcgga gaaagcggt caagaagaa 
      gaaagacca gcactcccc cactgccat cagtcccta 
      cgactccaa cgacaagcc ctgcatttg ctcgtgttg 
      ctacgccca actcccaaa cttcaaaag cttcctcca 
      ccacatgga gtcgcacgt aatctcctc gaacaactc 
      cctcctcgg cttctgccc cgtctgcgg cgagcctgg 
      ccgggaccc cgtctcgtt caccaacca catttttag 
      ccacgctat tgctcaagt acctggccg gtgttgcta 
      cacttgcaa gaagtcctt cgaaaggct ggaagagct 
      gcagaagca cctgttgga agtccacgt cgtgtcggt 
      gttcaagtg ttccatctg caacgatat tttcgatac 
      gaagatttc aatgcagtt gcatctgac gaacaaaca 
      cagcgacga atgcaagca cttcaagtg ctacttgtg 
      cacgaatca ggtgtttca cgatcgact ttcggcaga 
      attgcacat ctccatgaa gcactacca gcagttcac 
      gccctgtgt tggtgccaa ccaactgct acggtcgca 
      ataccagga gttggacac caggtttcg cgactataa 
      catgatttt ccagtgcac gttctgcca caaaacctt 
      caaggacca gtactcgca gtatatcca cattttgaa 
      ggagcacaa cgaaagcaa agagggtga aaaattcat 
      tttggattc agcaatgaa cccaaatcc gtccagatc 
      gcccaactt catgttccc caaaatcgt gacccctga 
      ccacgtgga gcccctgga atcggtcta cacttgtga 
      tatatgcaa ccgcagtga tataacctc cgaatcgga 
      cctaataaa ccacaagaa gctccacca cagcagcaa 
      gaacaagat cggtcccgt gagcctcca atgcgccta 
      ctgcaacga gtactgcaa gtcgcgcac ggacctgga 
      gaaccacat gaagaccca ccaagtgac ctgcggcaa 
      aggcaagca caagtgcaa catctgcga cgagattta 
      ctcctccac gttgaccct cgccgacca caagctcac 
      ccactgcaa gatcgtcga ggggaacag ttgcgtcca 
      gtgcaagtc ggtgctaac cgacgagaa ctccttcta 
      cagccacca gctgcagca cagcaaccc ggggaagcc 
      caactcgca gatctcgct cccagcgaa ctgcatcat 
      ctgctgcca gacgctgca gacggacgt ggagatcaa 
      gctccacgc caagttcca cctgaagca cctgacgca 
      gaaggagta catgtgcgg ggtctgcag caaagtgtt 
      cgacagcca ggggcagcc cgccaacga cgtcaacat 
      cgtcgtgtg caaagactg cgcgaagag tgaggacga 
      ggggaaaaa agtgccgaa acagttcgc gtgtatcca 
      gtgccccca gactttcga cagtgaaag tgacgtgca 
      aaaccacgc tgccatgca catgctgaa cgaggggac 
      gaacctgga gtgccgctt gtgcaagca ggtcttctc 
      gtcgcccct caagctgca gacgcacct aatcgagca 
      caacttcta cggaatgaa ccagtacag ttgttacgt 
      gtgttcctc ggttttcac ggcagctag cggcctgca 
      aagccacat aatcgggca tgggctgga cagcagacc 
      ctatgagtg ttcgcaatg ccagatgaa gttcttctt 
      ccgggccga gttggataa tcataggtt tgtgcacct 
      ccagaagag ctatccgat tcacaacgg gggcgagat 
      ctacgagaa caagccaag cccccggta taaaatctg 
      taaatactg ttcgaactc gtatctgga caactcgtt 
      tttcgtaga acatttgaa tcagtgtcc cttcaggat 
      aagtccgaa acgtgagac tttcgataa tgaagtaga 
      caataatat taaacaaga agtaacaga gatcgcgtc 
      ggaggaaaa agatgagca ataaaatcg ttgtaggac 
      tagctagga cttttatac gtactttta tttattaa
    • >TC004682 RB
      cagtgcgtg acaacttaa aacggagcg ataaatggg 
      gtcatgatt acaccaaac accgcatcc aaaagtgaa 
      acgctcaaa gttgattgc aaaaacggg ataaatgtg 
      ttggtttgt gcaacagtt ttggaataa tcaaaagaa 
      gttagatat gtccagacg gaagcaagc caatcctgc 
      caaattaga tcctcagga ggaggacga cctagaagc 
      acagcactt gagactagc cgaagatga aacggctgc 
      cagttccgc atccggtgg ttctggatg tggcgaaac 
      tcagccttt tgacgatga acaagtgca gggagatca 
      agatcaaga taccaaacc cggaccata caaatgcac 
      gagtcacac tgaccatct gcaattcac ttgtgctta 
      ttgtcctcg gcttttcaa gcacaagcg atcgagaga 
      tcgccacac gaagctcca cactgggga taaaaaata 
      taaatgtca gcagtgcga ttccgcctt ttcacgaag 
      tgatcatct taaaattca tatgaaaac acatgatag 
      tcgtaaacc atataaatg tggtacatg caatagggg 
      ctataacac cgccgcagc tctctcgtc gcatcaaca 
      aagtcactt gaaacaaga atctaggtc cggaagtcg 
      cacgagtgg aggaagcac tcccagccc cggactttt 
      ccggtgcac tcattgtgc cgaaacttt cggaaaacc 
      ggatctgct acagagtca tgtcgcaat ggtccacag 
      cgacacgga ctcgtcgct aagccaaac tccggagcc 
      cttatcaga gtatcaaaa ccaaatcga agacctgaa 
      aatcgtttg catgtactg ctcgaagga gttccccag 
      tttggagct gatgtacca gcacacaaa catcgccca 
      tcgtgacgt ccccaacgg cgttacaac ttccgtttc 
      cgtctcctc ccccgcgat ccaaaactc cccaaaacc 
      ggaaatttg ccaaaacgg aacaccaac ctacgcctg 
      tgatagatg cacgatgca attagactc gttgcagaa 
      cttgaaaaa ccacataaa caacgtaca ctggcgtcc 
      ggccctctc gccaaaccc cgtcaattt catggggat 
      tgagaaaag ctactcccc gttccagac ccaaccgac 
      cgacctgag ccgcaagaa gaaaagcga ggacagtgc 
      ggagaaagc ggtcaagaa gaagaaaga ccagcactc 
      ccccactgc catcagtcc ctacgactc caacgacaa 
      gccctgcat ttgctcgtg ttgctacgc ccaactccc 
      aaacttcaa aagcttcct ccaccacat ggagtcgca 
      cgtaatctc ctcgaacaa ctccctcct cggcttctg 
      ccccgtctg cggcgagcc tggccggga ccccgtctc 
      gttcaccaa ccacatttt tagccacgc tattgctca 
      agtacctgg ccggtgttg ctacacttg caagaagtc 
      cttcgaaag gctggaaga gctgcagaa gcacctgtt 
      ggaagtcca cgtcgtgtc ggtgttcaa gtgttccat 
      ctgcaacga tattttcga tacgaagat ttcaatgca 
      gttgcatct gacgaacaa acacagcga cgaatgcaa 
      gcacttcaa gtgctactt gtgcacgaa tcaggtgtt 
      tcacgatcg actttcggc agaattgca catctccat 
      gaagcacta ccagcagtt cacgccctg tgttggtgc 
      caaccaact gctacggtc gcaatacca ggagttgga 
      caccaggtt tcgcgacta taacatgat tttccagtg 
      cacgttctg ccacaaaac cttcaagga ccagtactc 
      gcagtatat ccacatttt gaaggagca caacgaaag 
      caaagaggg tgaaaaatt cattttgga ttcagcaat 
      gaacccaaa tccgtccag atcgcccaa cttcatgtt 
      ccccaaaat cgtgacccc tgaccacgt ggagcccct 
      ggaatcggt ctacacttg tgatatatg caaccgcag 
      tgatataac ctccgaatc ggacctaat aaaccacaa 
      gaagctcca ccacagcag caagaacaa gatcggtcc 
      cgtgagcct ccaatgcgc ctactgcaa cgagtactg 
      caagtcgcg cacggacct ggagaacca catgaagac 
      ccaccaagt gacctgcgg caaaggcaa gcacaagtg 
      caacatctg cgacgagat ttactcctc cacgttgac 
      cctcgccga ccacaagct cacccactg caagatcgt 
      cgaggggaa cagttgcgt ccagtgcaa gtcggtgct 
      aaccgacga gaactcctt ctacagcca ccagctgca 
      gcacagcaa cccggggaa gcccaactc gcagatctc 
      gctcccagc gaactgcat catctgctg ccagacgct 
      gcagacgga cgtggagat caagctcca cgccaagtt 
      ccacctgaa gcacctgac gcagaagga gtacatgtg 
      cggggtctg cagcaaagt gttcgacag ccaggggca 
      gcccgccaa cgacgtcaa catcgtcgt gtgcaaaga 
      ctgcgcgaa gagtgagga cgaggggaa aaaagtgcc 
      gaaacagtt cgcgtgtat ccagtgccc ccagacttt 
      cgacagtga aagtgacgt gcaaaacca cgctgccat 
      gcacatgct gaacgaggg gacgaacct ggagtgccg 
      cttgtgcaa gcaggtctt ctcgtcgcc cctcaagct 
      gcagacgca cctaatcga gcacaactt ctacggaat 
      gaaccagta cagttgtta cgtgtgttc ctcggtttt 
      cacggcagc tagcggcct gcaaagcca cataatcgg 
      gcatgggct ggacagcag accctatga gtgttcgca 
      atgccagat gaagttctt cttccgggc cgagttgga 
      taatcatag gtttgtgca cctccagaa gagctatcc 
      gattcacaa cgggggcga gatctacga gaacaagcc 
      aagcccccg gtataaaat ctgtaaata ctgttcgaa 
      ctcgtatct ggacaactc gtttttcgt agaacattt 
      gaatcagtg tcccttcag gataagtcc gaaacgtga 
      gactttcga taatgaagt agacaataa tattaaaca 
      agaagtaac agagatcgc gtcggagga aaaagatga 
      gcaataaaa tcgttgtag gactagcta ggactttta 
      tacgtactt ttatttatt aa
    • >TC004682 RC
      cagtgcgtg acaacttaa aacggagcg ataaatggg 
      gtcatgatt acaccaaac accgcatcc aaaagtgaa 
      acgctcaaa gttgattgc aaaaacggg ataaatgtg 
      ttggtttgt gcaacagtt ttggaataa tcaaaagaa 
      gttagatat gtccagacg gaagcaagc caatcctgc 
      caaattaga tcctcagga ggaggacga cctagaagc 
      acagcactt gagactgag tgacgacca gttcgccaa 
      aatgaaaat gctattcaa gggaaatag cagccgctt 
      ggagctact aatcgaaaa aattcagtc caccaaaga 
      gggcgaatt taaagtgcc agtagccga agatgaaac 
      ggctgccag ttccgcatc cggtggttc tggatgtgg 
      cgaaactca gccttttga cgatgaaca agtgcaggg 
      agatcaaga tcaagatac caaacccgg accatacaa 
      atgcacggt atgtggaga aaattattt aaaagaaaa 
      actgctcag ggagcacga acagagtca cactgacca 
      tctgcaatt cacttgtgc ttattgtcc tcggctttt 
      caagcacaa gcgatcgag agatcgcca cacgaagct 
      ccacactgg ggataaaaa atataaatg tcagcagtg 
      cgattccgc cttttcacg aagtgatca tcttaaaat 
      tcatatgaa aacacatga tagtcgtaa accatataa 
      atgtggtac atgcaatag gggctataa caccgccgc 
      agctctctc gtcgcatca acaaagtca cttgaaaca 
      agaatctag gtccggaag tcgcacgag tggaggaag 
      cactcccag ccccggact tttccggtg cactcattg 
      tgccgaaac tttcggaaa accggatct gctacagag 
      tcatgtcgc aatggtcca cagcgacac ggactcgtc 
      gctaagcca aactccgga gcccttatc agagtatca 
      aaaccaaat cgaagacct gaaaatcgt ttgcatgta 
      ctgctcgaa ggagttccc cagtttgga gctgatgta 
      ccagcacac aaacatcgc ccatcgtga cgtccccaa 
      cggcgttac aacttccgt ttccgtctc ctcccccgc 
      gatccaaaa ctccccaaa accggaaat ttgccaaaa 
      cggaacacc aacctacgc ctgtgatag atgcacgat 
      gcaattaga ctcgttgca gaacttgaa aaaccacat 
      aaacaacgt acactggcg tccggccct ctcgccaaa 
      ccccgtcaa tttcatggg gattgagaa aagctactc 
      cccgttcca gacccaacc gaccgacct gagccgcaa 
      gaagaaaag cgaggacag tgcggagaa agcggtcaa 
      gaagaagaa agaccagca ctcccccac tgccatcag 
      tccctacga ctccaacga caagccctg catttgctc 
      gtgttgcta cgcccaact cccaaactt caaaagctt 
      cctccacca catggagtc gcacgtaat ctcctcgaa 
      caactccct cctcggctt ctgccccgt ctgcggcga 
      gcctggccg ggaccccgt ctcgttcac caaccacat 
      ttttagcca cgctattgc tcaagtacc tggccggtg 
      ttgctacac ttgcaagaa gtccttcga aaggctgga 
      agagctgca gaagcacct gttggaagt ccacgtcgt 
      gtcggtgtt caagtgttc catctgcaa cgatatttt 
      cgatacgaa gatttcaat gcagttgca tctgacgaa 
      caaacacag cgacgaatg caagcactt caagtgcta 
      cttgtgcac gaatcaggt gtttcacga tcgactttc 
      ggcagaatt gcacatctc catgaagca ctaccagca 
      gttcacgcc ctgtgttgg tgccaacca actgctacg 
      gtcgcaata ccaggagtt ggacaccag gtttcgcga 
      ctataacat gattttcca gtgcacgtt ctgccacaa 
      aaccttcaa ggaccagta ctcgcagta tatccacat 
      tttgaagga gcacaacga aagcaaaga gggtgaaaa 
      attcatttt ggattcagc aatgaaccc aaatccgtc 
      cagatcgcc caacttcat gttccccaa aatcgtgac 
      ccctgacca cgtggagcc cctggaatc ggtctacac 
      ttgtgatat atgcaaccg cagtgatat aacctccga 
      atcggacct aataaacca caagaagct ccaccacag 
      cagcaagaa caagatcgg tcccgtgag cctccaatg 
      cgcctactg caacgagta ctgcaagtc gcgcacgga 
      cctggagaa ccacatgaa gacccacca agtgacctg 
      cggcaaagg caagcacaa gtgcaacat ctgcgacga 
      gatttactc ctccacgtt gaccctcgc cgaccacaa 
      gctcaccca ctgcaagat cgtcgaggg gaacagttg 
      cgtccagtg caagtcggt gctaaccga cgagaactc 
      cttctacag ccaccagct gcagcacag caacccggg 
      gaagcccaa ctcgcagat ctcgctccc agcgaactg 
      catcatctg ctgccagac gctgcagac ggacgtgga 
      gatcaagct ccacgccaa gttccacct gaagcacct 
      gacgcagaa ggagtacat gtgcggggt ctgcagcaa 
      agtgttcga cagccaggg gcagcccgc caacgacgt 
      caacatcgt cgtgtgcaa agactgcgc gaagagtga 
      ggacgaggg gaaaaaagt gccgaaaca gttcgcgtg 
      tatccagtg cccccagac tttcgacag tgaaagtga 
      cgtgcaaaa ccacgctgc catgcacat gctgaacga 
      ggggacgaa cctggagtg ccgcttgtg caagcaggt 
      cttctcgtc gcccctcaa gctgcagac gcacctaat 
      cgagcacaa cttctacgg aatgaacca gtacagttg 
      ttacgtgtg ttcctcggt tttcacggc agctagcgg 
      cctgcaaag ccacataat cgggcatgg gctggacag 
      cagacccta tgagtgttc gcaatgcca gatgaagtt 
      cttcttccg ggccgagtt ggataatca taggtttgt 
      gcacctcca gaagagcta tccgattca caacggggg 
      cgagatcta cgagaacaa gccaagccc ccggtataa 
      aatctgtaa atactgttc gaactcgta tctggacaa 
      ctcgttttt cgtagaaca tttgaatca gtgtccctt 
      caggataag tccgaaacg tgagacttt cgataatga 
      agtagacaa taatattaa acaagaagt aacagagat 
      cgcgtcgga ggaaaaaga tgagcaata aaatcgttg 
      taggactag ctaggactt ttatacgta cttttattt 
      attaa
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