Status
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Gene information
General information
TC008061 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4424032..4426116
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008061
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08113

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008061 RA
      MQPVKKLIE QQTGSARWK RYDEVIKRL RCPKEDEYT 
      QLLSSEDVI CDTPIFACK FAQQKSNEN LLALANEDG 
      KLAIQDTSK KHESYGIRA HNNAIFDLC WMFDNMKIV 
      TASGDHTAK LFDVAEADV REIRSFFGH TRSVKTIAF 
      RKEDPSVFA TGGRDGNII IWDTRTLGG SLVGKVDRT 
      IFNSHNTRT PVKTRKKCT CLSPSRASN AKSVTGLVF 
      QDDNTLISC GAGDGVIKI WDLRKNYLF YKQEPLPKN 
      KIPYGGTTT KNGFSSLII DRDCVRLYA NCLDNTIYS 
      YNVGTCNPE PVMRYTGHQ NSTFYIKAC LSRDGNYLI 
      SGSSDENAY IWNTSHSRP LVRLTGHVA EVTCVAWCE 
      RDDPTLVTC SDDMSHRIW RVGPEELPE NWEIIGKGH 
      AEVLPLIPV QSELKRPID EIIPISKKM SLACKKCDS 
      PSTSGVCQN CCSNNKRKI DEEISNETK RFHGELGPR 
      RLFQNLENN DEKTLTLNL PNYVIDGVA PHLNFSPPK 
      KKDQDWLTK LRTERKFKR DFETASTST PPKVAKLEA 
      TPKKCAPKS PLLRFFKVK CEGHSCTKS HSNCNVAAP 
      NTQSSQ
    CDS sequence
    • >TC008061 RA
      atgcagcca gtgaagaag ctaatcgag caacagacc 
      ggctcagcc cgctggaaa cgctacgac gaagtaatt 
      aagcgtctc cggtgcccc aaagaggac gaatacacc 
      cagttacta tcctccgaa gatgttatt tgtgatacc 
      ccgatcttt gcgtgcaaa ttcgcgcag caaaaatca 
      aacgaaaac ttgctggct ctcgctaac gaagacggc 
      aaactggcc attcaagac accagtaaa aagcacgag 
      tcttacggg atccgggcg cacaacaac gctattttc 
      gacctgtgc tggatgttc gacaatatg aagatcgtg 
      acggcgtca ggggaccac actgcgaag ctcttcgat 
      gtggcggag gccgacgtg agagaaatt cgctctttt 
      ttcggtcac acgcgctca gttaaaacc attgcgttc 
      cgcaaagag gacccgagt gtctttgcg actgggggg 
      cgcgacggc aatatcata atttgggac acgcggaca 
      ctcgggggt agcttggtc gggaaggtc gataggacc 
      atttttaac tcacacaat actagaact ccggtcaaa 
      acgcgcaaa aagtgcacg tgtttgagt ccctcgagg 
      gcgtcgaac gccaagtca gtgacgggg ttggtcttc 
      caagacgat aatacgttg atttcgtgc ggtgccggt 
      gatggggtt atcaaaatc tgggatttg cgcaagaat 
      tatctcttt tataagcag gagccgctg ccgaagaac 
      aaaatcccg tacgggggc accacgacg aagaacggc 
      ttcagcagt cttatcatc gatagggac tgtgtcagg 
      ctgtacgcc aattgttta gacaatacg atttatagt 
      tacaacgtt ggcacgtgt aatcctgag cccgtgatg 
      aggtacacg gggcaccag aacagcact ttttacatt 
      aaggcgtgt ctcagtcgc gatggcaat tatttgatt 
      agtgggagt agcgacgaa aacgcgtat atttggaac 
      acaagtcat tcgcgacct ttggtccga ttaaccgga 
      catgtggcg gaagtcacg tgtgtggca tggtgcgag 
      cgagacgac ccgacttta gtcacatgt tccgacgac 
      atgtcccac cggatctgg cgagtgggc ccggaggaa 
      ctgccggaa aattgggaa ataatcggg aaaggccac 
      gccgaagtg ttgccttta atcccggtc cagtccgaa 
      ctgaagcga ccaattgat gaaataatc ccgattagt 
      aaaaaaatg tcgcttgct tgcaaaaag tgcgacagt 
      ccttccacc agtggcgtg tgccaaaac tgttgttct 
      aacaacaaa cggaaaatc gatgaggaa atttccaac 
      gagactaag aggtttcac ggcgaattg gggccgagg 
      cgccttttc caaaatttg gaaaataat gatgagaag 
      acgctgact ttgaatttg cccaattat gtgattgat 
      ggggtggcc ccacatttg aatttttcg cctcccaag 
      aaaaaggat caagactgg ttgacgaaa ttgcgtacg 
      gagaggaag tttaagcgg gattttgaa acagcctcc 
      acttcgacg cccccgaaa gtggccaag ttggaggcg 
      acgccgaaa aaatgtgcg ccgaaaagc ccccttttg 
      aggtttttt aaagttaag tgtgaaggg cattcgtgt 
      acgaagtct cattcaaac tgtaatgtt gcggcgccg 
      aatacgcaa tccagtcaa taa
    mRNA sequence
    • >TC008061 RA
      aacgagaga aagagccac aatcacata agttggtgc 
      gtcccttga catttcccg ctttaacct cacattcta 
      gaaacatca acaactcaa aggccttgg tgaaatcgc 
      agcgaaatt gttactttt tgccccaaa atcgtgcaa 
      atgcagcca gtgaagaag ctaatcgag caacagacc 
      ggctcagcc cgctggaaa cgctacgac gaagtaatt 
      aagcgtctc cggtgcccc aaagaggac gaatacacc 
      cagttacta tcctccgaa gatgttatt tgtgatacc 
      ccgatcttt gcgtgcaaa ttcgcgcag caaaaatca 
      aacgaaaac ttgctggct ctcgctaac gaagacggc 
      aaactggcc attcaagac accagtaaa aagcacgag 
      tcttacggg atccgggcg cacaacaac gctattttc 
      gacctgtgc tggatgttc gacaatatg aagatcgtg 
      acggcgtca ggggaccac actgcgaag ctcttcgat 
      gtggcggag gccgacgtg agagaaatt cgctctttt 
      ttcggtcac acgcgctca gttaaaacc attgcgttc 
      cgcaaagag gacccgagt gtctttgcg actgggggg 
      cgcgacggc aatatcata atttgggac acgcggaca 
      ctcgggggt agcttggtc gggaaggtc gataggacc 
      atttttaac tcacacaat actagaact ccggtcaaa 
      acgcgcaaa aagtgcacg tgtttgagt ccctcgagg 
      gcgtcgaac gccaagtca gtgacgggg ttggtcttc 
      caagacgat aatacgttg atttcgtgc ggtgccggt 
      gatggggtt atcaaaatc tgggatttg cgcaagaat 
      tatctcttt tataagcag gagccgctg ccgaagaac 
      aaaatcccg tacgggggc accacgacg aagaacggc 
      ttcagcagt cttatcatc gatagggac tgtgtcagg 
      ctgtacgcc aattgttta gacaatacg atttatagt 
      tacaacgtt ggcacgtgt aatcctgag cccgtgatg 
      aggtacacg gggcaccag aacagcact ttttacatt 
      aaggcgtgt ctcagtcgc gatggcaat tatttgatt 
      agtgggagt agcgacgaa aacgcgtat atttggaac 
      acaagtcat tcgcgacct ttggtccga ttaaccgga 
      catgtggcg gaagtcacg tgtgtggca tggtgcgag 
      cgagacgac ccgacttta gtcacatgt tccgacgac 
      atgtcccac cggatctgg cgagtgggc ccggaggaa 
      ctgccggaa aattgggaa ataatcggg aaaggccac 
      gccgaagtg ttgccttta atcccggtc cagtccgaa 
      ctgaagcga ccaattgat gaaataatc ccgattagt 
      aaaaaaatg tcgcttgct tgcaaaaag tgcgacagt 
      ccttccacc agtggcgtg tgccaaaac tgttgttct 
      aacaacaaa cggaaaatc gatgaggaa atttccaac 
      gagactaag aggtttcac ggcgaattg gggccgagg 
      cgccttttc caaaatttg gaaaataat gatgagaag 
      acgctgact ttgaatttg cccaattat gtgattgat 
      ggggtggcc ccacatttg aatttttcg cctcccaag 
      aaaaaggat caagactgg ttgacgaaa ttgcgtacg 
      gagaggaag tttaagcgg gattttgaa acagcctcc 
      acttcgacg cccccgaaa gtggccaag ttggaggcg 
      acgccgaaa aaatgtgcg ccgaaaagc ccccttttg 
      aggtttttt aaagttaag tgtgaaggg cattcgtgt 
      acgaagtct cattcaaac tgtaatgtt gcggcgccg 
      aatacgcaa tccagtcaa taaattaag cacacaatg 
      tatctcata ttttaagac tttgtattg tagttttaa 
      gctgtcttg tttgttttt atgattttt tattatact 
      atgttttgt cttgttttt gacctgaaa ttaataaaa 
      aagttttat tttg
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