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Gene information
General information
TC008051 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4344932..4348146
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008051
    iBeetle screen iB_08111

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008051 RA
      MTWLSGLLL LSLIINTQS ESVDIDHSF ASRVYGTEL 
      LLLESDYQN QRTYKFEDF FWTGSGDGS EDDYQPTTI 
      YKTKTVLST IYLEPSTMI NTTTKCSFN CETPVTATE 
      EITPTPTQP PDDDFLSAD RHFWLLTVL KSDGKDPVI 
      IDLKNSLAK LYKTAFQRQ QERHLGIVN RDKRDAKNL 
      DKPVKVYIH NVEKSKTNG DEKIEVLYH VSVSGKPVD 
      AVTAAEDMT LITDDEVRS ELGYPFLIK AEPYLKPPA 
      PQGLSRSKN TWIFIGVSI VALLAILLV VAFLTLALT 
      KRKKRPPSG MDNRRQIFE RGALNEAKS DQSPTYINF 
      RNQPSNQTL RSVSVARPR SSISSSSAS STSLDISPL 
      MALKKQRTP PKPPRPKAA TNLKRIAPE LLDSDSNGS 
      KRESPETIY DPGVVSPKS YLSMPSVKA FPRGSMPEP 
      LNKVLEPVS VLHLDMPDD DGLVREGSR YGLTRHGSF 
      GAVEDPGVI GPVVWNMHC QRLQHGVSV DEGIDDLKV 
      PSNVSRMRK RFHDLLDDT FSLFGSRRE SPSKPPPIE 
      VKSHSAVNR PNDDIQPSV RPRPKTTDP RRIPPSPGS 
      IKPKGAWAS TAPSPLIRP LSADLTPKI NVQHCL
    CDS sequence
    • >TC008051 RA
      atgacatgg ctgagcggt ctcctcctc ttatcactg 
      attatcaac acccaatcg gagtcggtc gacattgac 
      cactctttt gcgtcacgt gtgtatgga acagagcta 
      ctcctcctg gagagcgat taccaaaat cagagaaca 
      tacaagttt gaggacttt ttctggact ggatcgggc 
      gatgggtcc gaagatgac taccaaccc acaaccatt 
      tacaaaacc aaaaccgtt ctttcgacc atttatctc 
      gaaccatcg actatgata aacacgacc actaaatgt 
      tcattcaat tgcgaaact ccagtcacc gcgactgaa 
      gaaatcacc ccaacccca acccaaccc cctgatgac 
      gatttctta agcgccgat cgtcacttc tggctgtta 
      acagttcta aagagcgac ggcaaagac cctgtcatt 
      attgatctc aagaatagt ttagctaaa ttatacaaa 
      accgcattt caaagacaa caagaacgc cacttaggg 
      atcgtaaac cgtgataaa agagacgcc aaaaatcta 
      gacaaaccg gtcaaagtc tacatccac aacgtggag 
      aagagtaaa acaaacggc gacgaaaaa atcgaagtc 
      ctttatcac gtctcagtt tcgggaaaa ccggtcgat 
      gctgtcacg gcggccgaa gacatgact ttgataaca 
      gacgacgaa gtgcggagc gaattgggt tatcctttc 
      ctcataaaa gccgaaccg tacttgaaa ccaccagcc 
      cctcaaggc ctctcccgt tcgaaaaac acgtggatt 
      tttatcggg gtttcaatc gtggccctt ttagcaatt 
      ctcctagtg gtggccttc ctgacccta gccctaaca 
      aagcgcaaa aagcgcccc ccgtctggc atggacaat 
      cggaggcag attttcgaa cggggggct ctcaacgaa 
      gctaaatcg gaccaatca cccacttat atcaacttc 
      cgaaaccag ccctcaaac cagacgctt cggagcgtc 
      tccgtagcc agaccccgc agctcaata agctcgagc 
      tcagccagt tccacaagt cttgatatt tccccgtta 
      atggctttg aaaaagcaa cgaacccct ccgaagccc 
      ccgcgtccc aaagctgcg actaatttg aaacgaatc 
      gcgccggag cttttggac tctgatagt aatgggagt 
      aagagggag tcaccggag acgatttac gacccgggg 
      gtggtcagc cccaagtcg tacttgtct atgccgtcg 
      gttaaggcg ttccccagg gggagtatg ccggaacct 
      ttgaataag gttttggag ccagtgagt gtcctacat 
      ttggatatg ccggacgat gatgggctt gtgagggaa 
      gggtcaagg tacggttta acgaggcat gggagcttt 
      ggggctgtg gaggatcca ggggttata gggcctgtt 
      gtttggaat atgcactgc cagaggttg caacacggg 
      gtgagcgta gacgagggc attgacgac ctaaaagtg 
      ccctccaac gtctcccga atgcgtaaa cgcttccac 
      gacctcctc gacgacacc ttcagcctg ttcggcagc 
      cggcgcgag agcccctcc aagccgccc cccatcgag 
      gtcaagagc cactccgcc gtcaacaga cccaacgac 
      gacatccag ccatcggtg cgcccccgc ccgaaaacc 
      accgatcct cgccgaata ccgccatcc ccgggaagt 
      attaaaccg aaaggtgcg tgggccagc acggccccg 
      tcgcccctc atccgcccc ctcagcgcc gacctaact 
      ccgaaaatc aacgtccag cattgcctt taa
    mRNA sequence
    • >TC008051 RA
      ggtgaacca tgagagact cctcactaa gggtagagt 
      cgtgcagtg gagtgcctt ccggggcca ccgaccctg 
      cgacgtctc ctcatcggt atgacatgg ctgagcggt 
      ctcctcctc ttatcactg attatcaac acccaatcg 
      gagtcggtc gacattgac cactctttt gcgtcacgt 
      gtgtatgga acagagcta ctcctcctg gagagcgat 
      taccaaaat cagagaaca tacaagttt gaggacttt 
      ttctggact ggatcgggc gatgggtcc gaagatgac 
      taccaaccc acaaccatt tacaaaacc aaaaccgtt 
      ctttcgacc atttatctc gaaccatcg actatgata 
      aacacgacc actaaatgt tcattcaat tgcgaaact 
      ccagtcacc gcgactgaa gaaatcacc ccaacccca 
      acccaaccc cctgatgac gatttctta agcgccgat 
      cgtcacttc tggctgtta acagttcta aagagcgac 
      ggcaaagac cctgtcatt attgatctc aagaatagt 
      ttagctaaa ttatacaaa accgcattt caaagacaa 
      caagaacgc cacttaggg atcgtaaac cgtgataaa 
      agagacgcc aaaaatcta gacaaaccg gtcaaagtc 
      tacatccac aacgtggag aagagtaaa acaaacggc 
      gacgaaaaa atcgaagtc ctttatcac gtctcagtt 
      tcgggaaaa ccggtcgat gctgtcacg gcggccgaa 
      gacatgact ttgataaca gacgacgaa gtgcggagc 
      gaattgggt tatcctttc ctcataaaa gccgaaccg 
      tacttgaaa ccaccagcc cctcaaggc ctctcccgt 
      tcgaaaaac acgtggatt tttatcggg gtttcaatc 
      gtggccctt ttagcaatt ctcctagtg gtggccttc 
      ctgacccta gccctaaca aagcgcaaa aagcgcccc 
      ccgtctggc atggacaat cggaggcag attttcgaa 
      cggggggct ctcaacgaa gctaaatcg gaccaatca 
      cccacttat atcaacttc cgaaaccag ccctcaaac 
      cagacgctt cggagcgtc tccgtagcc agaccccgc 
      agctcaata agctcgagc tcagccagt tccacaagt 
      cttgatatt tccccgtta atggctttg aaaaagcaa 
      cgaacccct ccgaagccc ccgcgtccc aaagctgcg 
      actaatttg aaacgaatc gcgccggag cttttggac 
      tctgatagt aatgggagt aagagggag tcaccggag 
      acgatttac gacccgggg gtggtcagc cccaagtcg 
      tacttgtct atgccgtcg gttaaggcg ttccccagg 
      gggagtatg ccggaacct ttgaataag gttttggag 
      ccagtgagt gtcctacat ttggatatg ccggacgat 
      gatgggctt gtgagggaa gggtcaagg tacggttta 
      acgaggcat gggagcttt ggggctgtg gaggatcca 
      ggggttata gggcctgtt gtttggaat atgcactgc 
      cagaggttg caacacggg gtgagcgta gacgagggc 
      attgacgac ctaaaagtg ccctccaac gtctcccga 
      atgcgtaaa cgcttccac gacctcctc gacgacacc 
      ttcagcctg ttcggcagc cggcgcgag agcccctcc 
      aagccgccc cccatcgag gtcaagagc cactccgcc 
      gtcaacaga cccaacgac gacatccag ccatcggtg 
      cgcccccgc ccgaaaacc accgatcct cgccgaata 
      ccgccatcc ccgggaagt attaaaccg aaaggtgcg 
      tgggccagc acggccccg tcgcccctc atccgcccc 
      ctcagcgcc gacctaact ccgaaaatc aacgtccag 
      cattgcctt taatcgcgg ccattaaga gcgaaatcg 
      agaagtgtt cgttgccgg ggagtacca ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY