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Gene information
General information
TC007499 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3704199..3710233
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007499
    iBeetle screen iB_08103

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007499 RA
      MNNVYVKPH TNFLPEAQP HTFYNQVTE NNNQIPTNG 
      DKSIAEVWT KYKFDTSNI TASVGNYNK TWATHEDPP 
      CEEKEEEVK VEDEPEEEE PKEKEEEVT TSPSHHARR 
      PMNAFLIFC KKHRPIVRE RFPNLENRG VTRILGEWW 
      ALLDSDDKA PYTGLAKEY KDAFLSANP DFKWYKLPA 
      PPLRTLNVR PSAHKVQSP VSSPQHVTE SEFTPGKLA 
      DESQLGSLT SLMNNFTPN IKSNENNNN NIEKNGDHD 
      YEDVPKSPV NFIKTALPL KPFKKRTFE KCTDNKNVV 
      RNIENDISV AQDGNMTNQ DLMDKVVDK IFTKPEDFD 
      RKEIRKSGR QCKGKRYEK FMVEGRLLG NKRDGKLTF 
      QRSSIENEL TFKLDQPEP ETPNLDHTI KRLAERTNM 
      RSVLEPSNQ KSEDRVRTT SENHETNFN LNQRISNLP 
      SLSYDVFHQ RKKDSKKRK NRIRTDSES KSKVAKVVK 
      EEPPVGSKK RKNKQSITH LKEECVGGD LLALTTLAE 
      VAANTEKIN SEPAVLKA
    CDS sequence
    • >TC007499 RA
      atgaataac gtttacgtc aaaccccat acgaatttt 
      ttacccgag gcccagcct cacactttt tacaaccaa 
      gttaccgaa aacaataac caaatccca accaacggt 
      gataaatca atcgcagaa gtttggacg aaatataaa 
      ttcgacacg agtaacatt actgcgagc gtcggtaat 
      tacaacaag acatgggcg acccatgag gaccccccg 
      tgcgaagaa aaagaagaa gaagtgaaa gtcgaagat 
      gagccggaa gaggaagag ccgaaagaa aaggaagag 
      gaagtgacc acctcgccc tcacaccac gcgaggcga 
      cccatgaac gccttcctg attttctgc aaaaagcat 
      cggcccatc gtccgggag cggttcccg aatttggag 
      aatcggggg gtcactagg atactcggg gagtggtgg 
      gcgttgctg gacagcgac gacaaggcc ccctacaca 
      ggcctagcc aaagagtac aaagatgcg ttcttgagc 
      gccaacccg gacttcaaa tggtacaaa cttccggct 
      ccaccgctg cggacgttg aacgttcgg ccttcagcc 
      cacaaagtc cagtcaccg gtttctagt ccacaacac 
      gtgaccgag tccgaattt actcccggg aaactggcc 
      gacgagtcc caattgggg agtctaacg tcgttgatg 
      aacaatttt acgccgaat atcaaatca aacgagaat 
      aataacaat aatatcgag aaaaacggc gatcacgat 
      tacgaggat gtgccgaaa agtccagtc aatttcatt 
      aaaactgct ctacctctt aaacctttc aagaaacgg 
      acgtttgag aagtgtact gacaataag aacgtcgtc 
      cggaacatt gaaaacgac atatcggtg gcacaggat 
      ggcaacatg acaaatcag gatctgatg gacaaggtg 
      gtcgacaag atcttcacc aaacccgag gatttcgac 
      agaaaggaa attcgaaaa tcgggccgc cagtgcaag 
      ggcaagcgg tacgagaag ttcatggtg gagggccgc 
      ctcttgggc aacaaacgc gacggcaag ctgactttc 
      cagcgcagc tcgatcgag aacgagctc accttcaag 
      ctggaccag ccggaaccg gaaacacca aacctagat 
      cacaccatc aaacgccta gccgaacgg accaacatg 
      cgaagcgtc ctcgaaccg tcgaaccaa aagagcgaa 
      gaccgcgtc cgcaccacc tccgagaac cacgaaacc 
      aacttcaac ctcaaccaa cgtatcagc aatttgccg 
      agtctgagc tacgacgta ttccaccaa aggaaaaag 
      gacagcaag aagcggaag aatcggata aggaccgac 
      agcgagtcc aagagcaaa gtggccaag gtggtgaaa 
      gaggagccg cctgtcggc tcgaagaag cggaaaaac 
      aagcagagc atcacgcat ttgaaggag gagtgcgtt 
      ggaggcgac ttgctggcg ctgacgacg ttggcggag 
      gtggcggcc aacacggag aagatcaac tcggaaccg 
      gcggttttg aaggcttaa
    mRNA sequence
    • >TC007499 RA
      cgcccacac gattgaaac aattgacgg tcgcccgtc 
      cgtaacata ttgggggtg tgttgcgaa aaattcgat 
      tgtttttcg ggggtagtc cgtctggaa aactgtgtc 
      acaaggcca aattttcat acacaaagg ggaaaaaat 
      ccgcgaatg aaattcatt gtcatgcgt ggtggggat 
      aaagtgggg aacagctga caagtgaca aactaaaca 
      ttacggaca tcttgcaaa tcgcagcag gcgtcgagt 
      ttgctccat tgtttttac tatgacaga aacgtaata 
      agtgggtgt catccagct atatgtgac cagtccggg 
      gttgttttt cttttgttt gtttcacag tgtactgtc 
      attttcccg tataatctc aaactggtg cgtgacgtc 
      atgtgattg cgttttcat ggagtgcta gattaagat 
      ggaaagtca ctagacgca aaatgaatt acttaacaa 
      tgaataacg tttacgtca aaccccata cgaattttt 
      tacccgagg cccagcctc acacttttt acaaccaag 
      ttaccgaaa acaataacc aaatcccaa ccaacggtg 
      ataaatcaa tcgcagaag tttggacga aatataaat 
      tcgacacga gtaacatta ctgcgagcg tcggtaatt 
      acaacaaga catgggcga cccatgagg accccccgt 
      gcgaagaaa aagaagaag aagtgaaag tcgaagatg 
      agccggaag aggaagagc cgaaagaaa aggaagagg 
      aagtgacca cctcgccct cacaccacg cgaggcgac 
      ccatgaacg ccttcctga ttttctgca aaaagcatc 
      ggcccatcg tccgggagc ggttcccga atttggaga 
      atcgggggg tcactagga tactcgggg agtggtggg 
      cgttgctgg acagcgacg acaaggccc cctacacag 
      gcctagcca aagagtaca aagatgcgt tcttgagcg 
      ccaacccgg acttcaaat ggtacaaac ttccggctc 
      caccgctgc ggacgttga acgttcggc cttcagccc 
      acaaagtcc agtcaccgg tttctagtc cacaacacg 
      tgaccgagt ccgaattta ctcccggga aactggccg 
      acgagtccc aattgggga gtctaacgt cgttgatga 
      acaatttta cgccgaata tcaaatcaa acgagaata 
      ataacaata atatcgaga aaaacggcg atcacgatt 
      acgaggatg tgccgaaaa gtccagtca atttcatta 
      aaactgctc tacctctta aacctttca agaaacgga 
      cgtttgaga agtgtactg acaataaga acgtcgtcc 
      ggaacattg aaaacgaca tatcggtgg cacaggatg 
      gcaacatga caaatcagg atctgatgg acaaggtgg 
      tcgacaaga tcttcacca aacccgagg atttcgaca 
      gaaaggaaa ttcgaaaat cgggccgcc agtgcaagg 
      gcaagcggt acgagaagt tcatggtgg agggccgcc 
      tcttgggca acaaacgcg acggcaagc tgactttcc 
      agcgcagct cgatcgaga acgagctca ccttcaagc 
      tggaccagc cggaaccgg aaacaccaa acctagatc 
      acaccatca aacgcctag ccgaacgga ccaacatgc 
      gaagcgtcc tcgaaccgt cgaaccaaa agagcgaag 
      accgcgtcc gcaccacct ccgagaacc acgaaacca 
      acttcaacc tcaaccaac gtatcagca atttgccga 
      gtctgagct acgacgtat tccaccaaa ggaaaaagg 
      acagcaaga agcggaaga atcggataa ggaccgaca 
      gcgagtcca agagcaaag tggccaagg tggtgaaag 
      aggagccgc ctgtcggct cgaagaagc ggaaaaaca 
      agcagagca tcacgcatt tgaaggagg agtgcgttg 
      gaggcgact tgctggcgc tgacgacgt tggcggagg 
      tggcggcca acacggaga agatcaact cggaaccgg 
      cggttttga aggcttaag aaattgatt ttcgcaata 
      attatggtt attccatgt actttatcg ggtattcta 
      gatgcatgt gcctctaac gtaattcaa aatataaac 
      aggaagtca catttctat tcggcgagc atttcaaga 
      gccttattc ccaagtatt agaattgtg atagctgtg 
      tattttttg aaggctacc tatatgtta agttttttt 
      tgtaatata gtcgttatg tttgttgtt atgtgttag 
      ccattttct aatcattta gaaccaaaa tcgttatca 
      cgttttaat tttattgag tatgtttat gttatttgt 
      agattttaa taagcgcaa taattttaa tgagatttc 
      atgttatgt tgtatgatc tgaccgtta gatctttgt 
      ttttttctt gagttagag attattatc ttgcactaa 
      tattcattc tagttgtaa gaaataata cttccttag 
      tcattttgt acaattatg aactagtct caaatttca 
      gcgtcagta ttcaattgg ttctttctc aacttcttc 
      agttcataa ttgtccaac taaagcgac caaatataa 
      ttaatattt taacaatct aagccttat agttgtagt 
      ttttaagat gaaaaacaa attatccac gttttatca 
      tttaaaaat aaattttat aaaagtact atgtgattg 
      accaattgt taatcgatc ttttgttct tatcagatt 
      tatggaatt gtaaggtac agacgtgat gcttggaat 
      gtgatactt tatttttct atcatgaaa tggacaggg 
      atataaaga tttatttat atgattgta tagattgtg 
      cctttttat ttatttata aatatttaa aatttatgc 
      ctttagaaa ggtaatcaa atatgtatc gattttaat 
      aaagtttat tacgtaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY