Status
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Gene information
General information
TC032789
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007975,TC007974
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3311430..3315749
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032789
    iBeetle screen iB_08100

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032789 RA
      MLPRRRRAG SETEPLVVT SDNHWTKES NLNYINSAY 
      PNNVSQDYY LEDDFQTEE YIEPEPVMA ARDRTSEFI 
      NTIQTLQGR SIQRAVAVR DPKKSKAIQ IHSEFMLIA 
      KNIGRNIAS TYTKLEKLT LFAMASYDI KNLEDVLNP 
      VIKKSDFVS ASVSRLTSA GDNYASLIL AVDITVKGQ 
      NNKIRTVAK LLPRNPYVQ KHFNSQCSF RNEVGYYQT 
      IIPCLKQFQ REQGISEVI NCVPDFFGA RFNLHPPST 
      KIDENAILL IKNIQDEGF VIIDRKIGF DANQTRLIL 
      SKLSQLHAT ALALQIHKP EIFATKIRP FLEEFTAFD 
      VGEEDSNRT QESLFDFIK DDAIFAEHL PRIRHILKA 
      NADFVIHRN NRNLKPSRF VTVSHGDLW VNNVMVKME 
      NGKTVDVKF VDFQMYEHT TLAKDVIFF LFTSVELSA 
      IETNLDAFL WWYYESFLR NLESLKCDV SEYTFEGFL 
      DEIGYILRT TELLHVLLM LRPIYADGS IKETEELKK 
      EDFVRYGFV PPKGCKEKA RYVVRECIR RDAMAIREI 
      KNLDELIFT FVDKSKFIG ATINKLTAA GENYCSELL 
      AVDISLRDG GKTKILRTV AKLLPENEF VRKLLKSER 
      TFLLEVKFY KTVVPCLIH FQKEQGFTR VFNAFPDFF 
      TSRFNLEQS LEKIDDNAV LLIKNLKDE GFGILDRKV 
      GFDFDQAKQ VLEKIAELH ATVFALKAL KPEVFESEF 
      GEVFEEFEM YESTEEDNR KFIDSLLAL IKDDDFCAK 
      NMDRIRGTL IRNGSCVKK KNDKEMKCR FASLCHYDL 
      WTNNIMVKA TDVKFIDFQ IYEYGSVTR DVLLFLFTS 
      VQLSVIQSE FDNLVKFYY ESFINYLRE LKCDVSGLT 
      FEEFLDEMG AMARTRELM HVLLMIQPI YRDEKDAKE 
      VKNMSKEDF LLAKRKSLF DDRTAEIQE LTYIIKGDL 
      SSLNQQIAQ LQDVSKKHK SYTTGKHLQ SHSSSVVLA 
      LQSKLATMS TDFKQILEV RTENLKHQK SRREQFSQG 
      GLPPPPVPS SSQGSLLLQ EQDQVSINL EGSALVPQR 
      TQMQAALMY DETDQYLQS RAETMQNIE STIVELGGI 
      FQQLAHMVK EQEEMVERI DTNVQDAEL NIEAAHAQI 
      LKYFQSVTS NRWLMIKIF GVLIFFFIF FVVFLA
    CDS sequence
    • >TC032789 RA
      atgctccct agaagacgg cgcgctggc tccgaaacc 
      gagcctcta gttgttaca agcgataat cactggacg 
      aaagaatct aacctcaat tacataaac agtgcatat 
      cccaacaat gtgtctcaa gattattac ttagaagac 
      gattttcaa accgaggag tacattgaa cctgaacca 
      gtgatggcg gcgagagac aggactagc gaatttatc 
      aatactata cagactcta caagggagg agtattcaa 
      agggctgtg gctgttagg gaccccaaa aaatccaaa 
      gccattcaa atacactcc gagtttatg ctaattgcg 
      aagaatatt ggccgaaat attgccagc acgtatacc 
      aagcttgag aaattaacc ctttttgca atggcctct 
      tacgatata aaaaacctt gaagatgtg ctcaatcca 
      gtcataaaa aaatccgat tttgtcagt gcttcggtc 
      tcaagactg acttcagca ggtgataat tacgccagt 
      ttaatactg gccgtagac ataacagtc aagggccaa 
      aataataaa attcgaaca gttgccaaa cttttgccc 
      cgaaaccca tacgttcag aaacatttc aacagtcag 
      tgttccttc cggaacgaa gttggttac taccaaacc 
      atcatccct tgtttgaaa caatttcaa agggagcaa 
      ggcataagc gaggtaatt aattgcgtc ccggatttt 
      ttcggtgcg aggtttaat ctacaccca ccaagcaca 
      aaaatcgac gaaaacgcg attcttctc attaaaaac 
      atccaagac gaaggtttc gtaatcatt gatcgaaaa 
      atcggcttt gatgccaac cagactcga ttaatttta 
      tcgaaacta tcacaactt cacgccact gctctagct 
      ttgcaaatc cacaaaccg gaaattttc gctacgaaa 
      attcgcccg tttttggaa gaattcaca gctttcgat 
      gtcggtgaa gaggatagt aaccgaaca caggaatct 
      ctatttgat tttataaaa gacgacgcg atttttgcc 
      gaacatttg ccaagaatt cggcacatt ctcaaagcg 
      aatgcggat tttgttatt catcgaaac aacagaaat 
      ttgaaaccg agtcgtttt gttactgtg agtcatggc 
      gatttgtgg gtcaacaat gtgatggtg aaaatggaa 
      aatggaaaa accgtcgat gtgaaattt gtcgatttt 
      caaatgtac gaacacacg actttggcc aaagatgtt 
      attttcttc ctattcacg agtgtggaa ttgtcggcg 
      attgagaca aatctcgat gcgtttttg tggtggtat 
      tatgagagt tttttgaga aatttggaa agtctgaag 
      tgtgacgtt tccgaatac acgtttgag ggttttttg 
      gacgaaatc ggttatatt ttgaggacg accgaattg 
      ctgcatgtt ttgttgatg ttgaggccc atttatgcc 
      gacggaagt attaaggaa actgaagaa ttaaagaaa 
      gaagatttc gttcggtat ggatttgta cccccgaag 
      gggtgtaag gaaaaagcg cgctacgtg gtccgtgaa 
      tgcatacgc cgagatgcg atggcgatt cgcgaaata 
      aaaaatctc gacgagtta attttcacg tttgtcgat 
      aaatcgaaa ttcatcggt gcgactatc aataaactg 
      actgccgct ggtgagaac tattgcagc gaattactg 
      gctgtggac atctcgcta cgtgacggc ggaaaaacg 
      aaaattctt cgaacagtg gcaaaatta ttacctgaa 
      aacgagttc gttcgaaaa ctgctaaaa agcgagcga 
      acgttcctc ctggaagta aaattctac aaaaccgtg 
      gtcccttgt ttgattcac ttccaaaag gaacaaggt 
      tttacgcga gtgtttaac gcattcccg gattttttc 
      acctcaagg tttaacctg gaacaaagt ttggaaaaa 
      atcgacgat aatgccgtt ttgttgatt aagaattta 
      aaagacgaa gggtttggg attttggac cgcaaagtt 
      gggttcgat tttgatcaa gcgaaacaa gttttggaa 
      aaaatagcc gaactgcac gccactgtt ttcgccttg 
      aaggccctc aaaccggag gtttttgag tcggaattt 
      ggggaggtt tttgaggag tttgaaatg tatgaatcg 
      acggaagaa gacaaccgg aagtttatc gattcgtta 
      ttggcgctg atcaaggat gatgatttt tgtgcaaaa 
      aacatggac cggatcagg ggtacactt atcagaaac 
      ggcagttgt gtcaaaaag aagaacgac aaagagatg 
      aagtgccgc tttgccagt ttgtgtcac tacgacttg 
      tggacaaat aatattatg gtcaaggct actgatgtg 
      aaatttatc gattttcaa atttacgaa tacggttct 
      gtaacaaga gatgtcctc ctgtttttg tttacgagt 
      gtccagtta tcggtgatt cagtcagaa tttgataat 
      ttggttaaa ttttattac gagagtttt atcaattat 
      ttgagggag ttaaagtgt gatgtttcg gggctcact 
      tttgaggag tttttggac gaaatgggg gcaatggcc 
      aggacaagg gaacttatg catgtgtta ctgatgata 
      caacccatt tatcgggac gaaaaagac gcaaaagaa 
      gtcaaaaat atgagcaaa gaagatttc ttactggct 
      aaaaggaaa tcgcttttc gacgaccgc acggccgaa 
      atccaagag ctgacctac atcatcaag ggcgacttg 
      agttcgctc aaccagcag atcgcccag ttacaggat 
      gtatcgaaa aagcacaag agttacacc accggcaaa 
      cacctgcag tcccattcc agcagcgtg gtcctggcc 
      ttacagtcg aagttggca acgatgtcg accgatttt 
      aagcaaatc ctcgaagtc cggacggaa aatctcaag 
      caccagaaa agccggcga gagcagttt tcccaggga 
      ggtctccct ccaccgcct gtgccctca tcgagccaa 
      gggagtttg ctgttgcaa gaacaggac caggtcagt 
      atcaacttg gaggggtcg gcgctggtg ccccaaagg 
      actcagatg caagcagcg ctgatgtat gacgagacg 
      gaccagtat ttgcaaagt cgggccgag actatgcag 
      aatatcgag tcgacgatt gtggagttg ggcggcatt 
      ttccagcag ttagcacac atggtgaag gaacaggag 
      gagatggtc gagaggata gacacgaac gtgcaagac 
      gctgagctg aacatcgaa gccgctcat gcccagatt 
      ttgaaatat ttccaatca gtgacgtcg aacaggtgg 
      ctcatgatt aaaattttc ggggttttg atcttcttt 
      tttatattt ttcgtcgtt tttctggcg tag
    mRNA sequence
    • >TC032789 RA
      tcggcgatg tgtcactcc tacttgtca aaattgcaa 
      ataattaaa taaggggta aattcagtg ttaagttat 
      ttcaagggg cgattttta gtgtcagaa ccccgttta 
      agtgtctct gtgctaact cgtgacttt ttaaattga 
      tttaatata aaatagttt ttcgagaac atatgtgaa 
      agtccagtt ttcgccccc attacgtgt ttaatctct 
      ctgtgtaaa actcaagaa tgcgatgct ccctagaag 
      acggcgcgc tggctccga aaccgagcc tctagttgt 
      tacaagcga taatcactg gacgaaaga atctaacct 
      caattacat aaacagtgc atatcccaa caatgtgtc 
      tcaagatta ttacttaga agacgattt tcaaaccga 
      ggagtacat tgaacctga accagtgat ggcggcgag 
      agacaggac tagcgaatt tatcaatac tatacagac 
      tctacaagg gaggagtat tcaaagggc tgtggctgt 
      tagggaccc caaaaaatc caaagccat tcaaataca 
      ctccgagtt tatgctaat tgcgaagaa tattggccg 
      aaatattgc cagcacgta taccaagct tgagaaatt 
      aaccctttt tgcaatggc ctcttacga tataaaaaa 
      ccttgaaga tgtgctcaa tccagtcat aaaaaaatc 
      cgattttgt cagtgcttc ggtctcaag actgacttc 
      agcaggtga taattacgc cagtttaat actggccgt 
      agacataac agtcaaggg ccaaaataa taaaattcg 
      aacagttgc caaactttt gccccgaaa cccatacgt 
      tcagaaaca tttcaacag tcagtgttc cttccggaa 
      cgaagttgg ttactacca aaccatcat cccttgttt 
      gaaacaatt tcaaaggga gcaaggcat aagcgaggt 
      aattaattg cgtcccgga ttttttcgg tgcgaggtt 
      taatctaca cccaccaag cacaaaaat cgacgaaaa 
      cgcgattct tctcattaa aaacatcca agacgaagg 
      tttcgtaat cattgatcg aaaaatcgg ctttgatgc 
      caaccagac tcgattaat tttatcgaa actatcaca 
      acttcacgc cactgctct agctttgca aatccacaa 
      accggaaat tttcgctac gaaaattcg cccgttttt 
      ggaagaatt cacagcttt cgatgtcgg tgaagagga 
      tagtaaccg aacacagga atctctatt tgattttat 
      aaaagacga cgcgatttt tgccgaaca tttgccaag 
      aattcggca cattctcaa agcgaatgc ggattttgt 
      tattcatcg aaacaacag aaatttgaa accgagtcg 
      ttttgttac tgtgagtca tggcgattt gtgggtcaa 
      caatgtgat ggtgaaaat ggaaaatgg aaaaaccgt 
      cgatgtgaa atttgtcga ttttcaaat gtacgaaca 
      cacgacttt ggccaaaga tgttatttt cttcctatt 
      cacgagtgt ggaattgtc ggcgattga gacaaatct 
      cgatgcgtt tttgtggtg gtattatga gagtttttt 
      gagaaattt ggaaagtct gaagtgtga cgtttccga 
      atacacgtt tgagggttt tttggacga aatcggtta 
      tattttgag gacgaccga attgctgca tgttttgtt 
      gatgttgag gcccattta tgccgacgg aagtattaa 
      ggaaactga agaattaaa gaaagaaga tttcgttcg 
      gtatggatt tgtaccccc gaaggggtg taaggaaaa 
      agcgcgcta cgtggtccg tgaatgcat acgccgaga 
      tgcgatggc gattcgcga aataaaaaa tctcgacga 
      gttaatttt cacgtttgt cgataaatc gaaattcat 
      cggtgcgac tatcaataa actgactgc cgctggtga 
      gaactattg cagcgaatt actggctgt ggacatctc 
      gctacgtga cggcggaaa aacgaaaat tcttcgaac 
      agtggcaaa attattacc tgaaaacga gttcgttcg 
      aaaactgct aaaaagcga gcgaacgtt cctcctgga 
      agtaaaatt ctacaaaac cgtggtccc ttgtttgat 
      tcacttcca aaaggaaca aggttttac gcgagtgtt 
      taacgcatt cccggattt tttcacctc aaggtttaa 
      cctggaaca aagtttgga aaaaatcga cgataatgc 
      cgttttgtt gattaagaa tttaaaaga cgaagggtt 
      tgggatttt ggaccgcaa agttgggtt cgattttga 
      tcaagcgaa acaagtttt ggaaaaaat agccgaact 
      gcacgccac tgttttcgc cttgaaggc cctcaaacc 
      ggaggtttt tgagtcgga atttgggga ggtttttga 
      ggagtttga aatgtatga atcgacgga agaagacaa 
      ccggaagtt tatcgattc gttattggc gctgatcaa 
      ggatgatga tttttgtgc aaaaaacat ggaccggat 
      caggggtac acttatcag aaacggcag ttgtgtcaa 
      aaagaagaa cgacaaaga gatgaagtg ccgctttgc 
      cagtttgtg tcactacga cttgtggac aaataatat 
      tatggtcaa ggctactga tgtgaaatt tatcgattt 
      tcaaattta cgaatacgg ttctgtaac aagagatgt 
      cctcctgtt tttgtttac gagtgtcca gttatcggt 
      gattcagtc agaatttga taatttggt taaatttta 
      ttacgagag ttttatcaa ttatttgag ggagttaaa 
      gtgtgatgt ttcggggct cacttttga ggagttttt 
      ggacgaaat gggggcaat ggccaggac aagggaact 
      tatgcatgt gttactgat gatacaacc catttatcg 
      ggacgaaaa agacgcaaa agaagtcaa aaatatgag 
      caaagaaga tttcttact ggctaaaag gaaatcgct 
      tttcgacga ccgcacggc cgaaatcca agagctgac 
      ctacatcat caagggcga cttgagttc gctcaacca 
      gcagatcgc ccagttaca ggatgtatc gaaaaagca 
      caagagtta caccaccgg caaacacct gcagtccca 
      ttccagcag cgtggtcct ggccttaca gtcgaagtt 
      ggcaacgat gtcgaccga ttttaagca aatcctcga 
      agtccggac ggaaaatct caagcacca gaaaagccg 
      gcgagagca gttttccca gggaggtct ccctccacc 
      gcctgtgcc ctcatcgag ccaagggag tttgctgtt 
      gcaagaaca ggaccaggt cagtatcaa cttggaggg 
      gtcggcgct ggtgcccca aaggactca gatgcaagc 
      agcgctgat gtatgacga gacggacca gtatttgca 
      aagtcgggc cgagactat gcagaatat cgagtcgac 
      gattgtgga gttgggcgg cattttcca gcagttagc 
      acacatggt gaaggaaca ggaggagat ggtcgagag 
      gatagacac gaacgtgca agacgctga gctgaacat 
      cgaagccgc tcatgccca gattttgaa atatttcca 
      atcagtgac gtcgaacag gtggctcat gattaaaat 
      tttcggggt tttgatctt cttttttat atttttcgt 
      cgtttttct ggcgtagta tcggcggga ctattttat 
      atcgttact aggtaggtc aaaagaagt gatattgtt 
      atgtgaaat tatcagaga caatatatt ataaataaa 
      aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY