| General information | |||
| TC032789
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3311430..3315749 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032789 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08100 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032789 RA
MLPRRRRAG SETEPLVVT SDNHWTKES NLNYINSAY PNNVSQDYY LEDDFQTEE YIEPEPVMA ARDRTSEFI NTIQTLQGR SIQRAVAVR DPKKSKAIQ IHSEFMLIA KNIGRNIAS TYTKLEKLT LFAMASYDI KNLEDVLNP VIKKSDFVS ASVSRLTSA GDNYASLIL AVDITVKGQ NNKIRTVAK LLPRNPYVQ KHFNSQCSF RNEVGYYQT IIPCLKQFQ REQGISEVI NCVPDFFGA RFNLHPPST KIDENAILL IKNIQDEGF VIIDRKIGF DANQTRLIL SKLSQLHAT ALALQIHKP EIFATKIRP FLEEFTAFD VGEEDSNRT QESLFDFIK DDAIFAEHL PRIRHILKA NADFVIHRN NRNLKPSRF VTVSHGDLW VNNVMVKME NGKTVDVKF VDFQMYEHT TLAKDVIFF LFTSVELSA IETNLDAFL WWYYESFLR NLESLKCDV SEYTFEGFL DEIGYILRT TELLHVLLM LRPIYADGS IKETEELKK EDFVRYGFV PPKGCKEKA RYVVRECIR RDAMAIREI KNLDELIFT FVDKSKFIG ATINKLTAA GENYCSELL AVDISLRDG GKTKILRTV AKLLPENEF VRKLLKSER TFLLEVKFY KTVVPCLIH FQKEQGFTR VFNAFPDFF TSRFNLEQS LEKIDDNAV LLIKNLKDE GFGILDRKV GFDFDQAKQ VLEKIAELH ATVFALKAL KPEVFESEF GEVFEEFEM YESTEEDNR KFIDSLLAL IKDDDFCAK NMDRIRGTL IRNGSCVKK KNDKEMKCR FASLCHYDL WTNNIMVKA TDVKFIDFQ IYEYGSVTR DVLLFLFTS VQLSVIQSE FDNLVKFYY ESFINYLRE LKCDVSGLT FEEFLDEMG AMARTRELM HVLLMIQPI YRDEKDAKE VKNMSKEDF LLAKRKSLF DDRTAEIQE LTYIIKGDL SSLNQQIAQ LQDVSKKHK SYTTGKHLQ SHSSSVVLA LQSKLATMS TDFKQILEV RTENLKHQK SRREQFSQG GLPPPPVPS SSQGSLLLQ EQDQVSINL EGSALVPQR TQMQAALMY DETDQYLQS RAETMQNIE STIVELGGI FQQLAHMVK EQEEMVERI DTNVQDAEL NIEAAHAQI LKYFQSVTS NRWLMIKIF GVLIFFFIF FVVFLA
- >TC032789 RA
atgctccct agaagacgg cgcgctggc tccgaaacc gagcctcta gttgttaca agcgataat cactggacg aaagaatct aacctcaat tacataaac agtgcatat cccaacaat gtgtctcaa gattattac ttagaagac gattttcaa accgaggag tacattgaa cctgaacca gtgatggcg gcgagagac aggactagc gaatttatc aatactata cagactcta caagggagg agtattcaa agggctgtg gctgttagg gaccccaaa aaatccaaa gccattcaa atacactcc gagtttatg ctaattgcg aagaatatt ggccgaaat attgccagc acgtatacc aagcttgag aaattaacc ctttttgca atggcctct tacgatata aaaaacctt gaagatgtg ctcaatcca gtcataaaa aaatccgat tttgtcagt gcttcggtc tcaagactg acttcagca ggtgataat tacgccagt ttaatactg gccgtagac ataacagtc aagggccaa aataataaa attcgaaca gttgccaaa cttttgccc cgaaaccca tacgttcag aaacatttc aacagtcag tgttccttc cggaacgaa gttggttac taccaaacc atcatccct tgtttgaaa caatttcaa agggagcaa ggcataagc gaggtaatt aattgcgtc ccggatttt ttcggtgcg aggtttaat ctacaccca ccaagcaca aaaatcgac gaaaacgcg attcttctc attaaaaac atccaagac gaaggtttc gtaatcatt gatcgaaaa atcggcttt gatgccaac cagactcga ttaatttta tcgaaacta tcacaactt cacgccact gctctagct ttgcaaatc cacaaaccg gaaattttc gctacgaaa attcgcccg tttttggaa gaattcaca gctttcgat gtcggtgaa gaggatagt aaccgaaca caggaatct ctatttgat tttataaaa gacgacgcg atttttgcc gaacatttg ccaagaatt cggcacatt ctcaaagcg aatgcggat tttgttatt catcgaaac aacagaaat ttgaaaccg agtcgtttt gttactgtg agtcatggc gatttgtgg gtcaacaat gtgatggtg aaaatggaa aatggaaaa accgtcgat gtgaaattt gtcgatttt caaatgtac gaacacacg actttggcc aaagatgtt attttcttc ctattcacg agtgtggaa ttgtcggcg attgagaca aatctcgat gcgtttttg tggtggtat tatgagagt tttttgaga aatttggaa agtctgaag tgtgacgtt tccgaatac acgtttgag ggttttttg gacgaaatc ggttatatt ttgaggacg accgaattg ctgcatgtt ttgttgatg ttgaggccc atttatgcc gacggaagt attaaggaa actgaagaa ttaaagaaa gaagatttc gttcggtat ggatttgta cccccgaag gggtgtaag gaaaaagcg cgctacgtg gtccgtgaa tgcatacgc cgagatgcg atggcgatt cgcgaaata aaaaatctc gacgagtta attttcacg tttgtcgat aaatcgaaa ttcatcggt gcgactatc aataaactg actgccgct ggtgagaac tattgcagc gaattactg gctgtggac atctcgcta cgtgacggc ggaaaaacg aaaattctt cgaacagtg gcaaaatta ttacctgaa aacgagttc gttcgaaaa ctgctaaaa agcgagcga acgttcctc ctggaagta aaattctac aaaaccgtg gtcccttgt ttgattcac ttccaaaag gaacaaggt tttacgcga gtgtttaac gcattcccg gattttttc acctcaagg tttaacctg gaacaaagt ttggaaaaa atcgacgat aatgccgtt ttgttgatt aagaattta aaagacgaa gggtttggg attttggac cgcaaagtt gggttcgat tttgatcaa gcgaaacaa gttttggaa aaaatagcc gaactgcac gccactgtt ttcgccttg aaggccctc aaaccggag gtttttgag tcggaattt ggggaggtt tttgaggag tttgaaatg tatgaatcg acggaagaa gacaaccgg aagtttatc gattcgtta ttggcgctg atcaaggat gatgatttt tgtgcaaaa aacatggac cggatcagg ggtacactt atcagaaac ggcagttgt gtcaaaaag aagaacgac aaagagatg aagtgccgc tttgccagt ttgtgtcac tacgacttg tggacaaat aatattatg gtcaaggct actgatgtg aaatttatc gattttcaa atttacgaa tacggttct gtaacaaga gatgtcctc ctgtttttg tttacgagt gtccagtta tcggtgatt cagtcagaa tttgataat ttggttaaa ttttattac gagagtttt atcaattat ttgagggag ttaaagtgt gatgtttcg gggctcact tttgaggag tttttggac gaaatgggg gcaatggcc aggacaagg gaacttatg catgtgtta ctgatgata caacccatt tatcgggac gaaaaagac gcaaaagaa gtcaaaaat atgagcaaa gaagatttc ttactggct aaaaggaaa tcgcttttc gacgaccgc acggccgaa atccaagag ctgacctac atcatcaag ggcgacttg agttcgctc aaccagcag atcgcccag ttacaggat gtatcgaaa aagcacaag agttacacc accggcaaa cacctgcag tcccattcc agcagcgtg gtcctggcc ttacagtcg aagttggca acgatgtcg accgatttt aagcaaatc ctcgaagtc cggacggaa aatctcaag caccagaaa agccggcga gagcagttt tcccaggga ggtctccct ccaccgcct gtgccctca tcgagccaa gggagtttg ctgttgcaa gaacaggac caggtcagt atcaacttg gaggggtcg gcgctggtg ccccaaagg actcagatg caagcagcg ctgatgtat gacgagacg gaccagtat ttgcaaagt cgggccgag actatgcag aatatcgag tcgacgatt gtggagttg ggcggcatt ttccagcag ttagcacac atggtgaag gaacaggag gagatggtc gagaggata gacacgaac gtgcaagac gctgagctg aacatcgaa gccgctcat gcccagatt ttgaaatat ttccaatca gtgacgtcg aacaggtgg ctcatgatt aaaattttc ggggttttg atcttcttt tttatattt ttcgtcgtt tttctggcg tag
- >TC032789 RA
tcggcgatg tgtcactcc tacttgtca aaattgcaa ataattaaa taaggggta aattcagtg ttaagttat ttcaagggg cgattttta gtgtcagaa ccccgttta agtgtctct gtgctaact cgtgacttt ttaaattga tttaatata aaatagttt ttcgagaac atatgtgaa agtccagtt ttcgccccc attacgtgt ttaatctct ctgtgtaaa actcaagaa tgcgatgct ccctagaag acggcgcgc tggctccga aaccgagcc tctagttgt tacaagcga taatcactg gacgaaaga atctaacct caattacat aaacagtgc atatcccaa caatgtgtc tcaagatta ttacttaga agacgattt tcaaaccga ggagtacat tgaacctga accagtgat ggcggcgag agacaggac tagcgaatt tatcaatac tatacagac tctacaagg gaggagtat tcaaagggc tgtggctgt tagggaccc caaaaaatc caaagccat tcaaataca ctccgagtt tatgctaat tgcgaagaa tattggccg aaatattgc cagcacgta taccaagct tgagaaatt aaccctttt tgcaatggc ctcttacga tataaaaaa ccttgaaga tgtgctcaa tccagtcat aaaaaaatc cgattttgt cagtgcttc ggtctcaag actgacttc agcaggtga taattacgc cagtttaat actggccgt agacataac agtcaaggg ccaaaataa taaaattcg aacagttgc caaactttt gccccgaaa cccatacgt tcagaaaca tttcaacag tcagtgttc cttccggaa cgaagttgg ttactacca aaccatcat cccttgttt gaaacaatt tcaaaggga gcaaggcat aagcgaggt aattaattg cgtcccgga ttttttcgg tgcgaggtt taatctaca cccaccaag cacaaaaat cgacgaaaa cgcgattct tctcattaa aaacatcca agacgaagg tttcgtaat cattgatcg aaaaatcgg ctttgatgc caaccagac tcgattaat tttatcgaa actatcaca acttcacgc cactgctct agctttgca aatccacaa accggaaat tttcgctac gaaaattcg cccgttttt ggaagaatt cacagcttt cgatgtcgg tgaagagga tagtaaccg aacacagga atctctatt tgattttat aaaagacga cgcgatttt tgccgaaca tttgccaag aattcggca cattctcaa agcgaatgc ggattttgt tattcatcg aaacaacag aaatttgaa accgagtcg ttttgttac tgtgagtca tggcgattt gtgggtcaa caatgtgat ggtgaaaat ggaaaatgg aaaaaccgt cgatgtgaa atttgtcga ttttcaaat gtacgaaca cacgacttt ggccaaaga tgttatttt cttcctatt cacgagtgt ggaattgtc ggcgattga gacaaatct cgatgcgtt tttgtggtg gtattatga gagtttttt gagaaattt ggaaagtct gaagtgtga cgtttccga atacacgtt tgagggttt tttggacga aatcggtta tattttgag gacgaccga attgctgca tgttttgtt gatgttgag gcccattta tgccgacgg aagtattaa ggaaactga agaattaaa gaaagaaga tttcgttcg gtatggatt tgtaccccc gaaggggtg taaggaaaa agcgcgcta cgtggtccg tgaatgcat acgccgaga tgcgatggc gattcgcga aataaaaaa tctcgacga gttaatttt cacgtttgt cgataaatc gaaattcat cggtgcgac tatcaataa actgactgc cgctggtga gaactattg cagcgaatt actggctgt ggacatctc gctacgtga cggcggaaa aacgaaaat tcttcgaac agtggcaaa attattacc tgaaaacga gttcgttcg aaaactgct aaaaagcga gcgaacgtt cctcctgga agtaaaatt ctacaaaac cgtggtccc ttgtttgat tcacttcca aaaggaaca aggttttac gcgagtgtt taacgcatt cccggattt tttcacctc aaggtttaa cctggaaca aagtttgga aaaaatcga cgataatgc cgttttgtt gattaagaa tttaaaaga cgaagggtt tgggatttt ggaccgcaa agttgggtt cgattttga tcaagcgaa acaagtttt ggaaaaaat agccgaact gcacgccac tgttttcgc cttgaaggc cctcaaacc ggaggtttt tgagtcgga atttgggga ggtttttga ggagtttga aatgtatga atcgacgga agaagacaa ccggaagtt tatcgattc gttattggc gctgatcaa ggatgatga tttttgtgc aaaaaacat ggaccggat caggggtac acttatcag aaacggcag ttgtgtcaa aaagaagaa cgacaaaga gatgaagtg ccgctttgc cagtttgtg tcactacga cttgtggac aaataatat tatggtcaa ggctactga tgtgaaatt tatcgattt tcaaattta cgaatacgg ttctgtaac aagagatgt cctcctgtt tttgtttac gagtgtcca gttatcggt gattcagtc agaatttga taatttggt taaatttta ttacgagag ttttatcaa ttatttgag ggagttaaa gtgtgatgt ttcggggct cacttttga ggagttttt ggacgaaat gggggcaat ggccaggac aagggaact tatgcatgt gttactgat gatacaacc catttatcg ggacgaaaa agacgcaaa agaagtcaa aaatatgag caaagaaga tttcttact ggctaaaag gaaatcgct tttcgacga ccgcacggc cgaaatcca agagctgac ctacatcat caagggcga cttgagttc gctcaacca gcagatcgc ccagttaca ggatgtatc gaaaaagca caagagtta caccaccgg caaacacct gcagtccca ttccagcag cgtggtcct ggccttaca gtcgaagtt ggcaacgat gtcgaccga ttttaagca aatcctcga agtccggac ggaaaatct caagcacca gaaaagccg gcgagagca gttttccca gggaggtct ccctccacc gcctgtgcc ctcatcgag ccaagggag tttgctgtt gcaagaaca ggaccaggt cagtatcaa cttggaggg gtcggcgct ggtgcccca aaggactca gatgcaagc agcgctgat gtatgacga gacggacca gtatttgca aagtcgggc cgagactat gcagaatat cgagtcgac gattgtgga gttgggcgg cattttcca gcagttagc acacatggt gaaggaaca ggaggagat ggtcgagag gatagacac gaacgtgca agacgctga gctgaacat cgaagccgc tcatgccca gattttgaa atatttcca atcagtgac gtcgaacag gtggctcat gattaaaat tttcggggt tttgatctt cttttttat atttttcgt cgtttttct ggcgtagta tcggcggga ctattttat atcgttact aggtaggtc aaaagaagt gatattgtt atgtgaaat tatcagaga caatatatt ataaataaa aca
