Status
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Gene information
General information
TC007924 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2811804..2813360
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007924
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08096

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007924 RA
      MKNQEIKIC RATLTVLKY SLIWPSEAD EMNPGKWYY 
IRVVTFILF TCPWVLSVF MHLIVSIRN NADIHLSED 
VALMVAFTG VYYMTIIYV KKQPKVAFL LRDLSYFQF 
GKPPGFDET ERILGFLSK LTFCYSVMA VVIYNYIKY 
RQKPECERM NKLKGLKEN CGMLTPTWW PFEINYSPA 
FQLIFLYIF TSTQVMMKL SLMISFNVL EMAHHIILR 
INHLKTMIL ESLDEQDYE ASKRKIKTC ILYHLEILG 
FAERMDDCF SNGMFAHLT ITAAICGCL EKQFVDGDN 
QLGSLLHIF GWILALFLA CLGGQHLIN ASETISDAI 
WSSKWYDAD LRLRKDLIF MMARSQVGL YLNVGGFGI 
LSYALFLSV IKMSYSILA MLTS
    CDS sequence
    • >TC007924 RA
      atgaagaat caggaaata aaaatatgt cgtgccact 
ttaactgtg ttaaaatac agtttgatt tggcccagc 
gaagcggac gaaatgaat ccaggaaag tggtattac 
atccgtgtc gtaactttc atacttttc acttgtcct 
tgggtctta tcagttttc atgcacctt attgtctcc 
ataagaaac aatgctgat atccatcta agcgaagac 
gttgccctg atggtggct ttcactggt gtctattac 
atgacgatc atttacgtg aaaaagcag ccaaaagtg 
gctttcctc ttgcgagat ttgtcctat tttcagttc 
ggcaaaccg cctggattt gacgaaacg gaacgaatt 
ttgggtttc ttatccaaa ttgaccttt tgttacagt 
gttatggca gttgtgatt tacaattac attaaatac 
cggcaaaaa ccagaatgt gaaagaatg aataaactg 
aaaggcttg aaggaaaac tgtgggatg ttgacaccg 
acatggtgg ccgtttgag atcaactat tcgccagcg 
tttcagttg atttttctt tacattttt acttcaacc 
caagtcatg atgaaactt tctttgatg atctcgttt 
aatgttctc gaaatggcg catcatatc attctgagg 
ataaatcac ttgaaaact atgattttg gaaagttta 
gatgagcaa gattatgaa gcgtctaaa cgaaaaata 
aaaacttgc attttgtat catttggaa atattgggt 
tttgccgaa cggatggat gattgtttt tcgaatgga 
atgtttgct catctcaca atcacagct gcgatttgt 
ggatgtttg gaaaaacaa ttcgtcgat ggtgataac 
caactaggg tctttgtta catattttt ggctggatt 
ttggctctg tttttggct tgtcttgga ggccaacat 
ttgattaat gctagtgaa acaatttct gacgccatt 
tggtcgtct aagtggtac gatgctgat ttacgtctt 
agaaaagat ttaatattt atgatggca aggtcacaa 
gtaggacta tacctgaac gttggaggg tttggaatt 
ctttcatac gcccttttt ttatcggta ataaaaatg 
tcatactca atcctcgct atgttaact tcttaa
    mRNA sequence
    • >TC007924 RA
      gcacttttg cgcaataat tttaatttg agagaagct 
tcagttaaa taaaatgaa gaatcagga aataaaaat 
atgtcgtgc cactttaac tgtgttaaa atacagttt 
gatttggcc cagcgaagc ggacgaaat gaatccagg 
aaagtggta ttacatccg tgtcgtaac tttcatact 
tttcacttg tccttgggt cttatcagt tttcatgca 
ccttattgt ctccataag aaacaatgc tgatatcca 
tctaagcga agacgttgc cctgatggt ggctttcac 
tggtgtcta ttacatgac gatcattta cgtgaaaaa 
gcagccaaa agtggcttt cctcttgcg agatttgtc 
ctattttca gttcggcaa accgcctgg atttgacga 
aacggaacg aattttggg tttcttatc caaattgac 
cttttgtta cagtgttat ggcagttgt gatttacaa 
ttacattaa ataccggca aaaaccaga atgtgaaag 
aatgaataa actgaaagg cttgaagga aaactgtgg 
gatgttgac accgacatg gtggccgtt tgagatcaa 
ctattcgcc agcgtttca gttgatttt tctttacat 
ttttacttc aacccaagt catgatgaa actttcttt 
gatgatctc gtttaatgt tctcgaaat ggcgcatca 
tatcattct gaggataaa tcacttgaa aactatgat 
tttggaaag tttagatga gcaagatta tgaagcgtc 
taaacgaaa aataaaaac ttgcatttt gtatcattt 
ggaaatatt gggttttgc cgaacggat ggatgattg 
tttttcgaa tggaatgtt tgctcatct cacaatcac 
agctgcgat ttgtggatg tttggaaaa acaattcgt 
cgatggtga taaccaact agggtcttt gttacatat 
ttttggctg gattttggc tctgttttt ggcttgtct 
tggaggcca acatttgat taatgctag tgaaacaat 
ttctgacgc catttggtc gtctaagtg gtacgatgc 
tgatttacg tcttagaaa agatttaat atttatgat 
ggcaaggtc acaagtagg actatacct gaacgttgg 
agggtttgg aattctttc atacgccct ttttttatc 
ggtaataaa aatgtcata ctcaatcct cgctatgtt 
aacttctta aattatact ttgaactaa ggcatttaa 
ttgtatttt taatcttag tactattat tattgtcac 
attattgca ctgtacaag gctatgtca ataaaaaca 
aaaaaaaac a

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY