Status
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Gene information
General information
TC007583 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2784473..2797372
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007583
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08095

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007583 RA
      MVASAVLGA AAGTGMALF VAMTIVVYR YYSHRRKLK 
      EWNSLDRMP FPTTPTRII NKPIYAVAQ TRLESWARA 
      AKERALGTT KHNNQENGS NTNCSNNSI EEFNVAKEQ 
      HIIQPPQNT SAQALTAEL MKMPLELCS APQGSHSLQ 
      HQSKSYPGG QPLLCRTPS VSSQSSLDS SASRQGHRG 
      SSPQIRTFA PDGKTPVHQ EAIHTSSYP LRNSSRSPS 
      PLRAASLDI RCSSPGTST VSGEEIRTP SPSQSSLAS 
      LTGGSVSSC NSPIPASPR TVGLGRCLS PLLIPSTRA 
      HTPEVNVAA PPASPLGAI QPDLYLRKD GPLFIGSSP 
      KAGPSLGRL HFRLKYDFD RSDLVVHLI EAHDLAGSD 
      QGGFNDPYV RLALSPEVD ARKRQTTIR RNDPNPFFD 
      QHFKFPISH EDLQSKTLI LQVFDYDRF SRNDVIGEV 
      RMSMDEFDV TSSIEVWGE ITKNKKPPE ELQEVLVSL 
      SYLPSAERL TVVLLKARN LFLPQEKDT IDPFVKVYL 
      LVNGKRIKK KKTAARKGT TNPVWNEAL TFSLSASNV 
      ANAAIEICI MDQANDLMG SNPLIGCCI IGPKEAGPE 
      RDHWLDMTQ SPRKAVACW HTMR
    CDS sequence
    • >TC007583 RA
      atggtggct tcggcagtg ctaggagcc gctgcgggg 
      accggaatg gcccttttc gtcgcgatg acaattgtt 
      gtttatcgc tattacagc caccggagg aaactcaag 
      gaatggaat tcgctggat cgaatgcct tttcccacc 
      accccgacc agaattatc aacaagcca atttatgct 
      gtcgcacaa acgaggctg gagtcgtgg gctagggcg 
      gccaaagaa cgcgccctt ggcaccacc aagcacaac 
      aaccaagaa aatggaagc aatacaaat tgtagcaat 
      aacagcatc gaggaattc aacgtagcg aaggagcaa 
      cacattatc cagcctccc caaaacacc tcagctcag 
      gcattaaca gctgaatta atgaaaatg ccgttggaa 
      ttatgttcc gcaccccaa ggctcgcat tcgttgcaa 
      caccagtcg aaatcgtat cctggggga cagccactt 
      ttatgccga acccccagc gtctcctca caaagtagc 
      ttagacagc agtgcatcc agacagggc caccgaggc 
      tcgtccccg cagatccgc accttcgcc ccggacggc 
      aaaacgccc gtccaccag gaagccatc cacacctcc 
      agctaccct ctgcgcaac tccagccgc tccccttcg 
      cctcttcga gcggcctcc ctggacatc cgctgctcg 
      tcccccgga acatcgacc gtgagtggt gaggaaatc 
      cggacgcct tctccgtcg caatccagc ttggcgtcg 
      ctcaccgga ggatcggtc agctcgtgc aattctccg 
      attccggcg tcgccaagg accgtcggc ctgggcagg 
      tgtctatcg ccgcttctg attccgtca acgagagca 
      cacacgccg gaagttaac gttgcggcg ccgccagcc 
      agtcccctg ggggctatt cagcccgat ctgtaccta 
      cggaaggac gggccgctc ttcataggc agcagcccc 
      aaagcgggg cccagttta ggaagactg cactttcgg 
      ctcaagtat gacttcgat aggagtgat ctggtggtt 
      catttgatt gaagcccac gacctggcg ggcagcgac 
      caagggggc tttaacgac ccctacgtc cgcctggcc 
      ctttcgccg gaagttgac gcgcgtaaa cgccaaacg 
      acaatccgt cgcaacgac ccaaatccg ttttttgat 
      caacacttc aaatttcca atatcgcat gaagatctg 
      caatcgaaa acgctcatc ctacaagtg tttgattac 
      gaccgattt tcccgaaac gatgtcatc ggcgaagtt 
      agaatgagc atggacgaa ttcgacgtt acatcgagc 
      atagaagtg tggggagaa atcaccaaa aataaaaaa 
      ccaccagaa gaattacaa gaagtgctc gtctcactc 
      agttatttg ccttcggct gaaagactg acggtggtg 
      ttactgaaa gcgagaaac ctgttcctg ccacaggag 
      aaggatacc atagatccg ttcgttaaa gtgtatctc 
      ctcgttaac ggcaagcga ataaaaaag aagaaaaca 
      gccgccaga aagggcacc acgaaccca gtctggaac 
      gaagcttta acctttagc ttgtcggct tcgaacgtg 
      gctaacgcc gcaattgag atctgcata atggaccaa 
      gcgaacgac ttgatgggg agcaacccg ctgataggc 
      tgctgcata atcggccct aaggaagca ggcccagag 
      agggaccac tggctggac atgactcaa agtccgcga 
      aaagctgtg gcttgctgg cacaccatg aggtaa
    mRNA sequence
    • >TC007583 RA
      acgcgtcaa caataaatt caaacagct gttccttaa 
      tttccacat ttccgacat cgtctccag acgcagctt 
      ccggaatgg tggcttcgg cagtgctag gagccgctg 
      cggggaccg gaatggccc ttttcgtcg cgatgacaa 
      ttgttgttt atcgctatt acagccacc ggaggaaac 
      tcaaggaat ggaattcgc tggatcgaa tgccttttc 
      ccaccaccc cgaccagaa ttatcaaca agccaattt 
      atgctgtcg cacaaacga ggctggagt cgtgggcta 
      gggcggcca aagaacgcg cccttggca ccaccaagc 
      acaacaacc aagaaaatg gaagcaata caaattgta 
      gcaataaca gcatcgagg aattcaacg tagcgaagg 
      agcaacaca ttatccagc ctccccaaa acacctcag 
      ctcaggcat taacagctg aattaatga aaatgccgt 
      tggaattat gttccgcac cccaaggct cgcattcgt 
      tgcaacacc agtcgaaat cgtatcctg ggggacagc 
      cacttttat gccgaaccc ccagcgtct cctcacaaa 
      gtagcttag acagcagtg catccagac agggccacc 
      gaggctcgt ccccgcaga tccgcacct tcgccccgg 
      acggcaaaa cgcccgtcc accaggaag ccatccaca 
      cctccagct accctctgc gcaactcca gccgctccc 
      cttcgcctc ttcgagcgg cctccctgg acatccgct 
      gctcgtccc ccggaacat cgaccgtga gtggtgagg 
      aaatccgga cgccttctc cgtcgcaat ccagcttgg 
      cgtcgctca ccggaggat cggtcagct cgtgcaatt 
      ctccgattc cggcgtcgc caaggaccg tcggcctgg 
      gcaggtgtc tatcgccgc ttctgattc cgtcaacga 
      gagcacaca cgccggaag ttaacgttg cggcgccgc 
      cagccagtc ccctggggg ctattcagc ccgatctgt 
      acctacgga aggacgggc cgctcttca taggcagca 
      gccccaaag cggggccca gtttaggaa gactgcact 
      ttcggctca agtatgact tcgatagga gtgatctgg 
      tggttcatt tgattgaag cccacgacc tggcgggca 
      gcgaccaag ggggcttta acgacccct acgtccgcc 
      tggcccttt cgccggaag ttgacgcgc gtaaacgcc 
      aaacgacaa tccgtcgca acgacccaa atccgtttt 
      ttgatcaac acttcaaat ttccaatat cgcatgaag 
      atctgcaat cgaaaacgc tcatcctac aagtgtttg 
      attacgacc gattttccc gaaacgatg tcatcggcg 
      aagttagaa tgagcatgg acgaattcg acgttacat 
      cgagcatag aagtgtggg gagaaatca ccaaaaata 
      aaaaaccac cagaagaat tacaagaag tgctcgtct 
      cactcagtt atttgcctt cggctgaaa gactgacgg 
      tggtgttac tgaaagcga gaaacctgt tcctgccac 
      aggagaagg ataccatag atccgttcg ttaaagtgt 
      atctcctcg ttaacggca agcgaataa aaaagaaga 
      aaacagccg ccagaaagg gcaccacga acccagtct 
      ggaacgaag ctttaacct ttagcttgt cggcttcga 
      acgtggcta acgccgcaa ttgagatct gcataatgg 
      accaagcga acgacttga tggggagca acccgctga 
      taggctgct gcataatcg gccctaagg aagcaggcc 
      cagagaggg accactggc tggacatga ctcaaagtc 
      cgcgaaaag ctgtggctt gctggcaca ccatgaggt 
      aaatcccaa tatgctcag ttgtggatc gattttaaa 
      cgaatttaa aagacaaaa atgggtacg aagataaat 
      ggcacaaga tgagtctac atgtccata atttaatgt 
      tattctatg ttaaacgtg atcatataa ttaagtgtg 
      tgtaacaat taagttaaa cattaagag cttttttag 
      agtctccaa actcgttat tttattgta agagttgac 
      ctgatttaa acggaacgg cctcgtcat tacaataaa 
      aaatggtaa ctaaattat acaaggaaa acttatgaa 
      ttacatgtg tccttttga atgttttta caagacaat 
      catttattt accaactca cttttttaa ctggcattt 
      ttaagaatt acgagggag agcggtcta aaaggatat 
      ttatatttg atattgtta ttctttgaa cactaactc 
      tagtaaaac gctgcactt tgtaattta acgattgta 
      aacttaaac aaccagaag aaacgctta aattgacat 
      cagggatgt taaatttac atattataa aagtcttat 
      gacgtccct ctcatactt atgatgaat gttgctatc 
      cttatattt acacttgaa ttatatcaa atgaagaat 
      atcataatc tatttacat gactgtata tcgaactgt 
      ttttaaata agaaaattt attaaaatc aaaactgta 
      cctataaac tataacggt atatcaatt attatatga 
      gtacctaaa tgttacgtg tttcttggt tcgacaccc 
      gtttttagt ttctaataa ttattgtaa acagtgtac 
      cttacagga ttgttgcaa taaatatca gtgagtatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY