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Gene information
General information
TC007583 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2784473..2797372
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007583
    iBeetle screen iB_08095

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007583 RA
      MVASAVLGA AAGTGMALF VAMTIVVYR YYSHRRKLK 
EWNSLDRMP FPTTPTRII NKPIYAVAQ TRLESWARA 
AKERALGTT KHNNQENGS NTNCSNNSI EEFNVAKEQ 
HIIQPPQNT SAQALTAEL MKMPLELCS APQGSHSLQ 
HQSKSYPGG QPLLCRTPS VSSQSSLDS SASRQGHRG 
SSPQIRTFA PDGKTPVHQ EAIHTSSYP LRNSSRSPS 
PLRAASLDI RCSSPGTST VSGEEIRTP SPSQSSLAS 
LTGGSVSSC NSPIPASPR TVGLGRCLS PLLIPSTRA 
HTPEVNVAA PPASPLGAI QPDLYLRKD GPLFIGSSP 
KAGPSLGRL HFRLKYDFD RSDLVVHLI EAHDLAGSD 
QGGFNDPYV RLALSPEVD ARKRQTTIR RNDPNPFFD 
QHFKFPISH EDLQSKTLI LQVFDYDRF SRNDVIGEV 
RMSMDEFDV TSSIEVWGE ITKNKKPPE ELQEVLVSL 
SYLPSAERL TVVLLKARN LFLPQEKDT IDPFVKVYL 
LVNGKRIKK KKTAARKGT TNPVWNEAL TFSLSASNV 
ANAAIEICI MDQANDLMG SNPLIGCCI IGPKEAGPE 
RDHWLDMTQ SPRKAVACW HTMR
    CDS sequence
    • >TC007583 RA
      atggtggct tcggcagtg ctaggagcc gctgcgggg 
accggaatg gcccttttc gtcgcgatg acaattgtt 
gtttatcgc tattacagc caccggagg aaactcaag 
gaatggaat tcgctggat cgaatgcct tttcccacc 
accccgacc agaattatc aacaagcca atttatgct 
gtcgcacaa acgaggctg gagtcgtgg gctagggcg 
gccaaagaa cgcgccctt ggcaccacc aagcacaac 
aaccaagaa aatggaagc aatacaaat tgtagcaat 
aacagcatc gaggaattc aacgtagcg aaggagcaa 
cacattatc cagcctccc caaaacacc tcagctcag 
gcattaaca gctgaatta atgaaaatg ccgttggaa 
ttatgttcc gcaccccaa ggctcgcat tcgttgcaa 
caccagtcg aaatcgtat cctggggga cagccactt 
ttatgccga acccccagc gtctcctca caaagtagc 
ttagacagc agtgcatcc agacagggc caccgaggc 
tcgtccccg cagatccgc accttcgcc ccggacggc 
aaaacgccc gtccaccag gaagccatc cacacctcc 
agctaccct ctgcgcaac tccagccgc tccccttcg 
cctcttcga gcggcctcc ctggacatc cgctgctcg 
tcccccgga acatcgacc gtgagtggt gaggaaatc 
cggacgcct tctccgtcg caatccagc ttggcgtcg 
ctcaccgga ggatcggtc agctcgtgc aattctccg 
attccggcg tcgccaagg accgtcggc ctgggcagg 
tgtctatcg ccgcttctg attccgtca acgagagca 
cacacgccg gaagttaac gttgcggcg ccgccagcc 
agtcccctg ggggctatt cagcccgat ctgtaccta 
cggaaggac gggccgctc ttcataggc agcagcccc 
aaagcgggg cccagttta ggaagactg cactttcgg 
ctcaagtat gacttcgat aggagtgat ctggtggtt 
catttgatt gaagcccac gacctggcg ggcagcgac 
caagggggc tttaacgac ccctacgtc cgcctggcc 
ctttcgccg gaagttgac gcgcgtaaa cgccaaacg 
acaatccgt cgcaacgac ccaaatccg ttttttgat 
caacacttc aaatttcca atatcgcat gaagatctg 
caatcgaaa acgctcatc ctacaagtg tttgattac 
gaccgattt tcccgaaac gatgtcatc ggcgaagtt 
agaatgagc atggacgaa ttcgacgtt acatcgagc 
atagaagtg tggggagaa atcaccaaa aataaaaaa 
ccaccagaa gaattacaa gaagtgctc gtctcactc 
agttatttg ccttcggct gaaagactg acggtggtg 
ttactgaaa gcgagaaac ctgttcctg ccacaggag 
aaggatacc atagatccg ttcgttaaa gtgtatctc 
ctcgttaac ggcaagcga ataaaaaag aagaaaaca 
gccgccaga aagggcacc acgaaccca gtctggaac 
gaagcttta acctttagc ttgtcggct tcgaacgtg 
gctaacgcc gcaattgag atctgcata atggaccaa 
gcgaacgac ttgatgggg agcaacccg ctgataggc 
tgctgcata atcggccct aaggaagca ggcccagag 
agggaccac tggctggac atgactcaa agtccgcga 
aaagctgtg gcttgctgg cacaccatg aggtaa
    mRNA sequence
    • >TC007583 RA
      acgcgtcaa caataaatt caaacagct gttccttaa 
tttccacat ttccgacat cgtctccag acgcagctt 
ccggaatgg tggcttcgg cagtgctag gagccgctg 
cggggaccg gaatggccc ttttcgtcg cgatgacaa 
ttgttgttt atcgctatt acagccacc ggaggaaac 
tcaaggaat ggaattcgc tggatcgaa tgccttttc 
ccaccaccc cgaccagaa ttatcaaca agccaattt 
atgctgtcg cacaaacga ggctggagt cgtgggcta 
gggcggcca aagaacgcg cccttggca ccaccaagc 
acaacaacc aagaaaatg gaagcaata caaattgta 
gcaataaca gcatcgagg aattcaacg tagcgaagg 
agcaacaca ttatccagc ctccccaaa acacctcag 
ctcaggcat taacagctg aattaatga aaatgccgt 
tggaattat gttccgcac cccaaggct cgcattcgt 
tgcaacacc agtcgaaat cgtatcctg ggggacagc 
cacttttat gccgaaccc ccagcgtct cctcacaaa 
gtagcttag acagcagtg catccagac agggccacc 
gaggctcgt ccccgcaga tccgcacct tcgccccgg 
acggcaaaa cgcccgtcc accaggaag ccatccaca 
cctccagct accctctgc gcaactcca gccgctccc 
cttcgcctc ttcgagcgg cctccctgg acatccgct 
gctcgtccc ccggaacat cgaccgtga gtggtgagg 
aaatccgga cgccttctc cgtcgcaat ccagcttgg 
cgtcgctca ccggaggat cggtcagct cgtgcaatt 
ctccgattc cggcgtcgc caaggaccg tcggcctgg 
gcaggtgtc tatcgccgc ttctgattc cgtcaacga 
gagcacaca cgccggaag ttaacgttg cggcgccgc 
cagccagtc ccctggggg ctattcagc ccgatctgt 
acctacgga aggacgggc cgctcttca taggcagca 
gccccaaag cggggccca gtttaggaa gactgcact 
ttcggctca agtatgact tcgatagga gtgatctgg 
tggttcatt tgattgaag cccacgacc tggcgggca 
gcgaccaag ggggcttta acgacccct acgtccgcc 
tggcccttt cgccggaag ttgacgcgc gtaaacgcc 
aaacgacaa tccgtcgca acgacccaa atccgtttt 
ttgatcaac acttcaaat ttccaatat cgcatgaag 
atctgcaat cgaaaacgc tcatcctac aagtgtttg 
attacgacc gattttccc gaaacgatg tcatcggcg 
aagttagaa tgagcatgg acgaattcg acgttacat 
cgagcatag aagtgtggg gagaaatca ccaaaaata 
aaaaaccac cagaagaat tacaagaag tgctcgtct 
cactcagtt atttgcctt cggctgaaa gactgacgg 
tggtgttac tgaaagcga gaaacctgt tcctgccac 
aggagaagg ataccatag atccgttcg ttaaagtgt 
atctcctcg ttaacggca agcgaataa aaaagaaga 
aaacagccg ccagaaagg gcaccacga acccagtct 
ggaacgaag ctttaacct ttagcttgt cggcttcga 
acgtggcta acgccgcaa ttgagatct gcataatgg 
accaagcga acgacttga tggggagca acccgctga 
taggctgct gcataatcg gccctaagg aagcaggcc 
cagagaggg accactggc tggacatga ctcaaagtc 
cgcgaaaag ctgtggctt gctggcaca ccatgaggt 
aaatcccaa tatgctcag ttgtggatc gattttaaa 
cgaatttaa aagacaaaa atgggtacg aagataaat 
ggcacaaga tgagtctac atgtccata atttaatgt 
tattctatg ttaaacgtg atcatataa ttaagtgtg 
tgtaacaat taagttaaa cattaagag cttttttag 
agtctccaa actcgttat tttattgta agagttgac 
ctgatttaa acggaacgg cctcgtcat tacaataaa 
aaatggtaa ctaaattat acaaggaaa acttatgaa 
ttacatgtg tccttttga atgttttta caagacaat 
catttattt accaactca cttttttaa ctggcattt 
ttaagaatt acgagggag agcggtcta aaaggatat 
ttatatttg atattgtta ttctttgaa cactaactc 
tagtaaaac gctgcactt tgtaattta acgattgta 
aacttaaac aaccagaag aaacgctta aattgacat 
cagggatgt taaatttac atattataa aagtcttat 
gacgtccct ctcatactt atgatgaat gttgctatc 
cttatattt acacttgaa ttatatcaa atgaagaat 
atcataatc tatttacat gactgtata tcgaactgt 
ttttaaata agaaaattt attaaaatc aaaactgta 
cctataaac tataacggt atatcaatt attatatga 
gtacctaaa tgttacgtg tttcttggt tcgacaccc 
gtttttagt ttctaataa ttattgtaa acagtgtac 
cttacagga ttgttgcaa taaatatca gtgagtatt
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