| General information | |||
| TC007583 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 2784473..2797372 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007583 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08095 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007583 RA
MVASAVLGA AAGTGMALF VAMTIVVYR YYSHRRKLK EWNSLDRMP FPTTPTRII NKPIYAVAQ TRLESWARA AKERALGTT KHNNQENGS NTNCSNNSI EEFNVAKEQ HIIQPPQNT SAQALTAEL MKMPLELCS APQGSHSLQ HQSKSYPGG QPLLCRTPS VSSQSSLDS SASRQGHRG SSPQIRTFA PDGKTPVHQ EAIHTSSYP LRNSSRSPS PLRAASLDI RCSSPGTST VSGEEIRTP SPSQSSLAS LTGGSVSSC NSPIPASPR TVGLGRCLS PLLIPSTRA HTPEVNVAA PPASPLGAI QPDLYLRKD GPLFIGSSP KAGPSLGRL HFRLKYDFD RSDLVVHLI EAHDLAGSD QGGFNDPYV RLALSPEVD ARKRQTTIR RNDPNPFFD QHFKFPISH EDLQSKTLI LQVFDYDRF SRNDVIGEV RMSMDEFDV TSSIEVWGE ITKNKKPPE ELQEVLVSL SYLPSAERL TVVLLKARN LFLPQEKDT IDPFVKVYL LVNGKRIKK KKTAARKGT TNPVWNEAL TFSLSASNV ANAAIEICI MDQANDLMG SNPLIGCCI IGPKEAGPE RDHWLDMTQ SPRKAVACW HTMR
- >TC007583 RA
atggtggct tcggcagtg ctaggagcc gctgcgggg accggaatg gcccttttc gtcgcgatg acaattgtt gtttatcgc tattacagc caccggagg aaactcaag gaatggaat tcgctggat cgaatgcct tttcccacc accccgacc agaattatc aacaagcca atttatgct gtcgcacaa acgaggctg gagtcgtgg gctagggcg gccaaagaa cgcgccctt ggcaccacc aagcacaac aaccaagaa aatggaagc aatacaaat tgtagcaat aacagcatc gaggaattc aacgtagcg aaggagcaa cacattatc cagcctccc caaaacacc tcagctcag gcattaaca gctgaatta atgaaaatg ccgttggaa ttatgttcc gcaccccaa ggctcgcat tcgttgcaa caccagtcg aaatcgtat cctggggga cagccactt ttatgccga acccccagc gtctcctca caaagtagc ttagacagc agtgcatcc agacagggc caccgaggc tcgtccccg cagatccgc accttcgcc ccggacggc aaaacgccc gtccaccag gaagccatc cacacctcc agctaccct ctgcgcaac tccagccgc tccccttcg cctcttcga gcggcctcc ctggacatc cgctgctcg tcccccgga acatcgacc gtgagtggt gaggaaatc cggacgcct tctccgtcg caatccagc ttggcgtcg ctcaccgga ggatcggtc agctcgtgc aattctccg attccggcg tcgccaagg accgtcggc ctgggcagg tgtctatcg ccgcttctg attccgtca acgagagca cacacgccg gaagttaac gttgcggcg ccgccagcc agtcccctg ggggctatt cagcccgat ctgtaccta cggaaggac gggccgctc ttcataggc agcagcccc aaagcgggg cccagttta ggaagactg cactttcgg ctcaagtat gacttcgat aggagtgat ctggtggtt catttgatt gaagcccac gacctggcg ggcagcgac caagggggc tttaacgac ccctacgtc cgcctggcc ctttcgccg gaagttgac gcgcgtaaa cgccaaacg acaatccgt cgcaacgac ccaaatccg ttttttgat caacacttc aaatttcca atatcgcat gaagatctg caatcgaaa acgctcatc ctacaagtg tttgattac gaccgattt tcccgaaac gatgtcatc ggcgaagtt agaatgagc atggacgaa ttcgacgtt acatcgagc atagaagtg tggggagaa atcaccaaa aataaaaaa ccaccagaa gaattacaa gaagtgctc gtctcactc agttatttg ccttcggct gaaagactg acggtggtg ttactgaaa gcgagaaac ctgttcctg ccacaggag aaggatacc atagatccg ttcgttaaa gtgtatctc ctcgttaac ggcaagcga ataaaaaag aagaaaaca gccgccaga aagggcacc acgaaccca gtctggaac gaagcttta acctttagc ttgtcggct tcgaacgtg gctaacgcc gcaattgag atctgcata atggaccaa gcgaacgac ttgatgggg agcaacccg ctgataggc tgctgcata atcggccct aaggaagca ggcccagag agggaccac tggctggac atgactcaa agtccgcga aaagctgtg gcttgctgg cacaccatg aggtaa
- >TC007583 RA
acgcgtcaa caataaatt caaacagct gttccttaa tttccacat ttccgacat cgtctccag acgcagctt ccggaatgg tggcttcgg cagtgctag gagccgctg cggggaccg gaatggccc ttttcgtcg cgatgacaa ttgttgttt atcgctatt acagccacc ggaggaaac tcaaggaat ggaattcgc tggatcgaa tgccttttc ccaccaccc cgaccagaa ttatcaaca agccaattt atgctgtcg cacaaacga ggctggagt cgtgggcta gggcggcca aagaacgcg cccttggca ccaccaagc acaacaacc aagaaaatg gaagcaata caaattgta gcaataaca gcatcgagg aattcaacg tagcgaagg agcaacaca ttatccagc ctccccaaa acacctcag ctcaggcat taacagctg aattaatga aaatgccgt tggaattat gttccgcac cccaaggct cgcattcgt tgcaacacc agtcgaaat cgtatcctg ggggacagc cacttttat gccgaaccc ccagcgtct cctcacaaa gtagcttag acagcagtg catccagac agggccacc gaggctcgt ccccgcaga tccgcacct tcgccccgg acggcaaaa cgcccgtcc accaggaag ccatccaca cctccagct accctctgc gcaactcca gccgctccc cttcgcctc ttcgagcgg cctccctgg acatccgct gctcgtccc ccggaacat cgaccgtga gtggtgagg aaatccgga cgccttctc cgtcgcaat ccagcttgg cgtcgctca ccggaggat cggtcagct cgtgcaatt ctccgattc cggcgtcgc caaggaccg tcggcctgg gcaggtgtc tatcgccgc ttctgattc cgtcaacga gagcacaca cgccggaag ttaacgttg cggcgccgc cagccagtc ccctggggg ctattcagc ccgatctgt acctacgga aggacgggc cgctcttca taggcagca gccccaaag cggggccca gtttaggaa gactgcact ttcggctca agtatgact tcgatagga gtgatctgg tggttcatt tgattgaag cccacgacc tggcgggca gcgaccaag ggggcttta acgacccct acgtccgcc tggcccttt cgccggaag ttgacgcgc gtaaacgcc aaacgacaa tccgtcgca acgacccaa atccgtttt ttgatcaac acttcaaat ttccaatat cgcatgaag atctgcaat cgaaaacgc tcatcctac aagtgtttg attacgacc gattttccc gaaacgatg tcatcggcg aagttagaa tgagcatgg acgaattcg acgttacat cgagcatag aagtgtggg gagaaatca ccaaaaata aaaaaccac cagaagaat tacaagaag tgctcgtct cactcagtt atttgcctt cggctgaaa gactgacgg tggtgttac tgaaagcga gaaacctgt tcctgccac aggagaagg ataccatag atccgttcg ttaaagtgt atctcctcg ttaacggca agcgaataa aaaagaaga aaacagccg ccagaaagg gcaccacga acccagtct ggaacgaag ctttaacct ttagcttgt cggcttcga acgtggcta acgccgcaa ttgagatct gcataatgg accaagcga acgacttga tggggagca acccgctga taggctgct gcataatcg gccctaagg aagcaggcc cagagaggg accactggc tggacatga ctcaaagtc cgcgaaaag ctgtggctt gctggcaca ccatgaggt aaatcccaa tatgctcag ttgtggatc gattttaaa cgaatttaa aagacaaaa atgggtacg aagataaat ggcacaaga tgagtctac atgtccata atttaatgt tattctatg ttaaacgtg atcatataa ttaagtgtg tgtaacaat taagttaaa cattaagag cttttttag agtctccaa actcgttat tttattgta agagttgac ctgatttaa acggaacgg cctcgtcat tacaataaa aaatggtaa ctaaattat acaaggaaa acttatgaa ttacatgtg tccttttga atgttttta caagacaat catttattt accaactca cttttttaa ctggcattt ttaagaatt acgagggag agcggtcta aaaggatat ttatatttg atattgtta ttctttgaa cactaactc tagtaaaac gctgcactt tgtaattta acgattgta aacttaaac aaccagaag aaacgctta aattgacat cagggatgt taaatttac atattataa aagtcttat gacgtccct ctcatactt atgatgaat gttgctatc cttatattt acacttgaa ttatatcaa atgaagaat atcataatc tatttacat gactgtata tcgaactgt ttttaaata agaaaattt attaaaatc aaaactgta cctataaac tataacggt atatcaatt attatatga gtacctaaa tgttacgtg tttcttggt tcgacaccc gtttttagt ttctaataa ttattgtaa acagtgtac cttacagga ttgttgcaa taaatatca gtgagtatt
