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Gene information
General information
TC007893 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2286102..2287891
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007893
    iBeetle screen iB_08090

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007893 RA
      MEENDTENR VNFITCVKW VKKGVAKSQ PEKVQLSKA 
      ELVQVIKAT KKKLRVTNE ENAQGESSA QDEFNLENY 
      DEQDEGTAE ALGISSLAD LQNNEEENF SESDDSDKE 
      DEVIKPNDN LILVGHVEG DASSLEVYI YNEEEESLY 
      VHHDILLPA FPLCFEWLD YEPNAPKGS YCAIGSMTP 
      IIQVWDLDI INCIEPAFN LGRAASIKK NRPHVGHKD 
      AVLTLAWNK TYEHVLASG SVDKTILLW DLENKTPST 
      TISAFKDKV QCMQWHKLE AQTLLAGSS DKKAKIFDC 
      RNPETHQTW KINGEVETL VWNPLQPFS FFAGSDTGN 
      LQYFDCRKG QVWAVEAHE KEVTGLVVS PQCPGLLVT 
      SSPDGTIKI WDYTENEAT FVFEKDFSL GTVQCLDLS 
      PDLPFVIAA GGDNKSNNF LVHDLRNID VVKHKFGDR 
      QLEELVAES TEESMNTD
    CDS sequence
    • >TC007893 RA
      atggaagaa aacgacacc gaaaatcga gttaatttc 
      atcacttgc gtcaagtgg gtgaagaaa ggagtggca 
      aaaagccaa cccgaaaag gtccaattg tccaaagcc 
      gagctcgta caagtcata aaagccaca aaaaagaaa 
      cttcgagtc acaaatgag gaaaatgct caaggtgaa 
      tcatcggct caagatgag ttcaatttg gaaaattat 
      gacgaacaa gacgaaggc accgctgag gctttgggg 
      ataagttca ctggctgat ttacaaaat aatgaggaa 
      gaaaacttc tctgaatca gacgattcg gacaaagaa 
      gacgaagtt atcaaacct aacgataat ttaattcta 
      gttggacat gtggaaggt gacgcaagt tcgctagaa 
      gtgtacatt tacaacgag gaggaagaa tcactttac 
      gtccaccac gatatctta cttcccgcc tttccactt 
      tgtttcgaa tggttggac tacgaaccc aacgcccca 
      aaaggcagt tactgtgct ataggttcc atgaccccc 
      attatccaa gtctgggac ttggacata attaactgc 
      atagaaccc gcgtttaat ttaggccga gccgcaagt 
      attaaaaaa aaccgacct cacgtcggc cacaaagac 
      gcagtttta actttggct tggaacaaa acctacgaa 
      cacgtctta gctagcggt tcagttgac aaaactata 
      cttttgtgg gacttggag aacaaaact ccaagtacg 
      accatttcc gcgttcaaa gacaaggtc cagtgcatg 
      cagtggcac aaactggaa gcgcaaact ttactagct 
      gggtctagc gacaagaaa gcaaaaatt ttcgattgt 
      cgcaaccct gagacacat cagacgtgg aaaataaat 
      ggggaggtt gaaactctg gtctggaac ccgctacaa 
      ccgttttca tttttcgcc ggtagtgat accggaaat 
      ttgcaatat tttgattgt agaaaaggt caagtgtgg 
      gctgttgaa gcccatgaa aaggaagtc actgggttg 
      gttgtgagt ccgcaatgt cccggttta ttagtcaca 
      tcgtcgcca gatgggacg ataaaaatt tgggactac 
      accgaaaat gaagccact ttcgtgttc gaaaaagat 
      tttagttta gggacagtc cagtgcctg gatttgtcg 
      cccgatttg ccgtttgtc atagcggca ggaggggat 
      aataaatct aataatttt ctagttcac gatttgaga 
      aacatcgat gttgtaaaa cacaagttt ggagaccgg 
      caattagaa gaattagtc gcagaaagt acagaagaa 
      agcatgaac actgattag
    mRNA sequence
    • >TC007893 RA
      ttctttaaa aaattgccg aaaacatac aaactgagg 
      ttatttttt ataacctta aatacattc gacacgtgt 
      gtttgacac ctagctcgt tttactaaa attttggct 
      aaatggaag aaaacgaca ccgaaaatc gagttaatt 
      tcatcactt gcgtcaagt gggtgaaga aaggagtgg 
      caaaaagcc aacccgaaa aggtccaat tgtccaaag 
      ccgagctcg tacaagtca taaaagcca caaaaaaga 
      aacttcgag tcacaaatg aggaaaatg ctcaaggtg 
      aatcatcgg ctcaagatg agttcaatt tggaaaatt 
      atgacgaac aagacgaag gcaccgctg aggctttgg 
      ggataagtt cactggctg atttacaaa ataatgagg 
      aagaaaact tctctgaat cagacgatt cggacaaag 
      aagacgaag ttatcaaac ctaacgata atttaattc 
      tagttggac atgtggaag gtgacgcaa gttcgctag 
      aagtgtaca tttacaacg aggaggaag aatcacttt 
      acgtccacc acgatatct tacttcccg cctttccac 
      tttgtttcg aatggttgg actacgaac ccaacgccc 
      caaaaggca gttactgtg ctataggtt ccatgaccc 
      ccattatcc aagtctggg acttggaca taattaact 
      gcatagaac ccgcgttta atttaggcc gagccgcaa 
      gtattaaaa aaaaccgac ctcacgtcg gccacaaag 
      acgcagttt taactttgg cttggaaca aaacctacg 
      aacacgtct tagctagcg gttcagttg acaaaacta 
      tacttttgt gggacttgg agaacaaaa ctccaagta 
      cgaccattt ccgcgttca aagacaagg tccagtgca 
      tgcagtggc acaaactgg aagcgcaaa ctttactag 
      ctgggtcta gcgacaaga aagcaaaaa ttttcgatt 
      gtcgcaacc ctgagacac atcagacgt ggaaaataa 
      atggggagg ttgaaactc tggtctgga acccgctac 
      aaccgtttt catttttcg ccggtagtg ataccggaa 
      atttgcaat attttgatt gtagaaaag gtcaagtgt 
      gggctgttg aagcccatg aaaaggaag tcactgggt 
      tggttgtga gtccgcaat gtcccggtt tattagtca 
      catcgtcgc cagatggga cgataaaaa tttgggact 
      acaccgaaa atgaagcca ctttcgtgt tcgaaaaag 
      attttagtt tagggacag tccagtgcc tggatttgt 
      cgcccgatt tgccgtttg tcatagcgg caggagggg 
      ataataaat ctaataatt ttctagttc acgatttga 
      gaaacatcg atgttgtaa aacacaagt ttggagacc 
      ggcaattag aagaattag tcgcagaaa gtacagaag 
      aaagcatga acactgatt agattttaa tcaaagtgt 
      aataaaact caataaaac ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY