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Gene information
General information
TC007893 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2286102..2287891
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007893
    iBeetle screen iB_08090

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007893 RA
      MEENDTENR VNFITCVKW VKKGVAKSQ PEKVQLSKA 
ELVQVIKAT KKKLRVTNE ENAQGESSA QDEFNLENY 
DEQDEGTAE ALGISSLAD LQNNEEENF SESDDSDKE 
DEVIKPNDN LILVGHVEG DASSLEVYI YNEEEESLY 
VHHDILLPA FPLCFEWLD YEPNAPKGS YCAIGSMTP 
IIQVWDLDI INCIEPAFN LGRAASIKK NRPHVGHKD 
AVLTLAWNK TYEHVLASG SVDKTILLW DLENKTPST 
TISAFKDKV QCMQWHKLE AQTLLAGSS DKKAKIFDC 
RNPETHQTW KINGEVETL VWNPLQPFS FFAGSDTGN 
LQYFDCRKG QVWAVEAHE KEVTGLVVS PQCPGLLVT 
SSPDGTIKI WDYTENEAT FVFEKDFSL GTVQCLDLS 
PDLPFVIAA GGDNKSNNF LVHDLRNID VVKHKFGDR 
QLEELVAES TEESMNTD
    CDS sequence
    • >TC007893 RA
      atggaagaa aacgacacc gaaaatcga gttaatttc 
atcacttgc gtcaagtgg gtgaagaaa ggagtggca 
aaaagccaa cccgaaaag gtccaattg tccaaagcc 
gagctcgta caagtcata aaagccaca aaaaagaaa 
cttcgagtc acaaatgag gaaaatgct caaggtgaa 
tcatcggct caagatgag ttcaatttg gaaaattat 
gacgaacaa gacgaaggc accgctgag gctttgggg 
ataagttca ctggctgat ttacaaaat aatgaggaa 
gaaaacttc tctgaatca gacgattcg gacaaagaa 
gacgaagtt atcaaacct aacgataat ttaattcta 
gttggacat gtggaaggt gacgcaagt tcgctagaa 
gtgtacatt tacaacgag gaggaagaa tcactttac 
gtccaccac gatatctta cttcccgcc tttccactt 
tgtttcgaa tggttggac tacgaaccc aacgcccca 
aaaggcagt tactgtgct ataggttcc atgaccccc 
attatccaa gtctgggac ttggacata attaactgc 
atagaaccc gcgtttaat ttaggccga gccgcaagt 
attaaaaaa aaccgacct cacgtcggc cacaaagac 
gcagtttta actttggct tggaacaaa acctacgaa 
cacgtctta gctagcggt tcagttgac aaaactata 
cttttgtgg gacttggag aacaaaact ccaagtacg 
accatttcc gcgttcaaa gacaaggtc cagtgcatg 
cagtggcac aaactggaa gcgcaaact ttactagct 
gggtctagc gacaagaaa gcaaaaatt ttcgattgt 
cgcaaccct gagacacat cagacgtgg aaaataaat 
ggggaggtt gaaactctg gtctggaac ccgctacaa 
ccgttttca tttttcgcc ggtagtgat accggaaat 
ttgcaatat tttgattgt agaaaaggt caagtgtgg 
gctgttgaa gcccatgaa aaggaagtc actgggttg 
gttgtgagt ccgcaatgt cccggttta ttagtcaca 
tcgtcgcca gatgggacg ataaaaatt tgggactac 
accgaaaat gaagccact ttcgtgttc gaaaaagat 
tttagttta gggacagtc cagtgcctg gatttgtcg 
cccgatttg ccgtttgtc atagcggca ggaggggat 
aataaatct aataatttt ctagttcac gatttgaga 
aacatcgat gttgtaaaa cacaagttt ggagaccgg 
caattagaa gaattagtc gcagaaagt acagaagaa 
agcatgaac actgattag
    mRNA sequence
    • >TC007893 RA
      ttctttaaa aaattgccg aaaacatac aaactgagg 
ttatttttt ataacctta aatacattc gacacgtgt 
gtttgacac ctagctcgt tttactaaa attttggct 
aaatggaag aaaacgaca ccgaaaatc gagttaatt 
tcatcactt gcgtcaagt gggtgaaga aaggagtgg 
caaaaagcc aacccgaaa aggtccaat tgtccaaag 
ccgagctcg tacaagtca taaaagcca caaaaaaga 
aacttcgag tcacaaatg aggaaaatg ctcaaggtg 
aatcatcgg ctcaagatg agttcaatt tggaaaatt 
atgacgaac aagacgaag gcaccgctg aggctttgg 
ggataagtt cactggctg atttacaaa ataatgagg 
aagaaaact tctctgaat cagacgatt cggacaaag 
aagacgaag ttatcaaac ctaacgata atttaattc 
tagttggac atgtggaag gtgacgcaa gttcgctag 
aagtgtaca tttacaacg aggaggaag aatcacttt 
acgtccacc acgatatct tacttcccg cctttccac 
tttgtttcg aatggttgg actacgaac ccaacgccc 
caaaaggca gttactgtg ctataggtt ccatgaccc 
ccattatcc aagtctggg acttggaca taattaact 
gcatagaac ccgcgttta atttaggcc gagccgcaa 
gtattaaaa aaaaccgac ctcacgtcg gccacaaag 
acgcagttt taactttgg cttggaaca aaacctacg 
aacacgtct tagctagcg gttcagttg acaaaacta 
tacttttgt gggacttgg agaacaaaa ctccaagta 
cgaccattt ccgcgttca aagacaagg tccagtgca 
tgcagtggc acaaactgg aagcgcaaa ctttactag 
ctgggtcta gcgacaaga aagcaaaaa ttttcgatt 
gtcgcaacc ctgagacac atcagacgt ggaaaataa 
atggggagg ttgaaactc tggtctgga acccgctac 
aaccgtttt catttttcg ccggtagtg ataccggaa 
atttgcaat attttgatt gtagaaaag gtcaagtgt 
gggctgttg aagcccatg aaaaggaag tcactgggt 
tggttgtga gtccgcaat gtcccggtt tattagtca 
catcgtcgc cagatggga cgataaaaa tttgggact 
acaccgaaa atgaagcca ctttcgtgt tcgaaaaag 
attttagtt tagggacag tccagtgcc tggatttgt 
cgcccgatt tgccgtttg tcatagcgg caggagggg 
ataataaat ctaataatt ttctagttc acgatttga 
gaaacatcg atgttgtaa aacacaagt ttggagacc 
ggcaattag aagaattag tcgcagaaa gtacagaag 
aaagcatga acactgatt agattttaa tcaaagtgt 
aataaaact caataaaac ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY