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Gene information
General information
TC007853 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1586353..1587617
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007853
    iBeetle screen iB_08085

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007853 RA
      MNTEEITIT VPWGHLAAK IWGNKNDPL VLVFHGIMD 
      NAGSFDRLI PLLPKSFCY ICFDLPGHG KSSHFPPFF 
      PAYTLNNVL VYKIIVQYF KKEKYTILG HSYGGQIAF 
      LFAQLYPEY VEKLIMLDT IHLFPVHAG QFRQNLRDK 
      MDFCIELDQ KIKTGTRPT YTYAEALQK LQDQRANGY 
      ISREAAEAL LQRAIERTD DGKYAFTVD QRMKQFINP 
      IHDFRYILE TLKKFPVTC PVLMVLGRD SELQQMYMK 
      GVTNFFKKY RNIRITYVD GHHDVHNNS PEIVAPHVS 
      KFLVVKKAK L
    CDS sequence
    • >TC007853 RA
      atgaacact gaagaaatc acaataacg gtaccttgg 
      ggccacctg gcagccaaa atatggggc aacaaaaac 
      gaccctctc gtgctcgtt ttccacgga atcatggac 
      aacgcgggc tcatttgac cgcctaatt ccgctcctc 
      cccaaatca ttttgctac atttgcttc gacttgccc 
      ggtcacgga aaatcgagc catttcccc ccgtttttc 
      ccagcctac acccttaat aacgtccta gtttacaaa 
      ataatagtc cagtatttc aaaaaagaa aagtacacg 
      attttgggg cacagctac gggggccag atcgccttt 
      ttattcgcc caactgtac cccgaatat gtggaaaag 
      ctgataatg ctcgacact atccacttg ttccccgtc 
      catgcgggc caattccgg caaaacctc agggacaag 
      atggacttt tgcatcgaa ttggaccag aaaattaaa 
      actgggacc cgtccaacg tacacttac gccgaagcc 
      ttacagaaa ctgcaggac caaagggcc aacggctac 
      atcagtcgt gaggcggcc gaggcgctg ctccaaagg 
      gcgattgag aggaccgac gacgggaaa tacgccttt 
      acggtcgac cagaggatg aaacagttt ataaacccg 
      atccacgat tttcggtac attctcgaa acgttgaaa 
      aagtttccg gtcacgtgc cccgttttg atggttttg 
      gggcgcgac tcagagttg cagcaaatg tacatgaaa 
      ggggtcaca aacttcttt aaaaagtat agaaacata 
      cggataact tatgtggat gggcaccac gatgtgcat 
      aataatagc cccgaaatt gtggctccg cacgtgtcc 
      aagtttttg gtggtgaaa aaagcgaaa ttgtaa
    mRNA sequence
    • >TC007853 RA
      cacgtaatg agcccacaa tgacttaac atttttttt 
      gcccaaaat ctgttatca aaaatgaga cgctttatt 
      gctgtttta ttgtaatga tctggagac gtgacgcaa 
      gagcaaacc ttgtaactt gtacctgtg ataaaacaa 
      taataaata agcaaaatt agttattag aacaatatt 
      aaaaaatcg tgttacgaa taaccgggt gccacatcc 
      acacaagta attgataag tgataacca taatgaaca 
      ctgaagaaa tcacaataa cggtacctt ggggccacc 
      tggcagcca aaatatggg gcaacaaaa acgaccctc 
      tcgtgctcg ttttccacg gaatcatgg acaacgcgg 
      gctcatttg accgcctaa ttccgctcc tccccaaat 
      cattttgct acatttgct tcgacttgc ccggtcacg 
      gaaaatcga gccatttcc ccccgtttt tcccagcct 
      acaccctta ataacgtcc tagtttaca aaataatag 
      tccagtatt tcaaaaaag aaaagtaca cgattttgg 
      ggcacagct acgggggcc agatcgcct ttttattcg 
      cccaactgt accccgaat atgtggaaa agctgataa 
      tgctcgaca ctatccact tgttccccg tccatgcgg 
      gccaattcc ggcaaaacc tcagggaca agatggact 
      tttgcatcg aattggacc agaaaatta aaactggga 
      cccgtccaa cgtacactt acgccgaag ccttacaga 
      aactgcagg accaaaggg ccaacggct acatcagtc 
      gtgaggcgg ccgaggcgc tgctccaaa gggcgattg 
      agaggaccg acgacggga aatacgcct ttacggtcg 
      accagagga tgaaacagt ttataaacc cgatccacg 
      attttcggt acattctcg aaacgttga aaaagtttc 
      cggtcacgt gccccgttt tgatggttt tggggcgcg 
      actcagagt tgcagcaaa tgtacatga aaggggtca 
      caaacttct ttaaaaagt atagaaaca tacggataa 
      cttatgtgg atgggcacc acgatgtgc ataataata 
      gccccgaaa ttgtggctc cgcacgtgt ccaagtttt 
      tggtggtga aaaaagcga aattgtaat gttgggtaa 
      agtttaatt aattctagg ggcatttat acacaataa 
      agactgtga tagtctctt agtaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY