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Gene information
General information
TC032733
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007801
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 733331..738537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032733
    iBeetle screen iB_08074

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032733 RA
      MSDPDVVLN LTIFNSDWI YKNMLKKQI TENKQALER 
      MLAMSDFLR KQLHVLENH RCESMRLIC SSHRQFETY 
      LRSQKDQIV TSLQNEYDG VKMSILTKA QTVDQTVRD 
      LVVKLNKMQ LMTENNGET ASGIDMEMA ARHGLLLNG 
      DCMDNNVFF NDFASYLPD DFHSNGTDS SDATDRPLE 
      PPKSKCHVN PDLSLSSSN PDDNILLPS HDNNVVNSM 
      QLCKTFGQK GNGSGQFNS PHGFCIGFN EDIVVADSS 
      NHRVQIFDK YGRYKSQFG ITGRHDGYL WHPRKVVWL 
      QKKCCYVVC DRGYERSRM QVFTKEGKF YKKINVHFI 
      DIVAGIAVT SNGEIVVVD SVTPTLFIA DENDFLRTL 
      DCSPFIQEP SDIAVVGKE FYICDFKGH AIVVMDDDG 
      NFLRKFGGT ETTMFPNGI DISSTGDVI VGDSHGNYF 
      HVAVYSKQG NFLAQYRCP VVKVSRCCG LKMTSEGRL 
      VTVDKNYHH VIVFESLFG PPEAFS
    CDS sequence
    • >TC032733 RA
      atgtccgat ccggacgtg gtcttgaac ttgaccatt 
      ttcaactct gactggatc tacaagaac atgctgaaa 
      aaacagatc actgagaac aaacaagcg cttgaacgc 
      atgctggca atgtccgat tttttgcgc aagcaattg 
      cacgttttg gaaaatcat cgctgtgaa agtatgcgc 
      ttgatttgt tcgtcgcat agacagttt gaaacttat 
      ttgcggagt cagaaagac cagattgtt acctctttg 
      caaaatgaa tacgacggg gtcaagatg tccattctt 
      acgaaggcg caaactgtt gatcagact gttagggat 
      ttggtggtc aagttgaat aaaatgcag ctgatgacg 
      gaaaataat ggcgaaact gcgagtggg attgatatg 
      gagatggcg gctagacat gggcttttg ctgaatggt 
      gattgtatg gataataat gtgtttttc aacgacttt 
      gcttcgtat ttgccggat gatttccac tcgaatggc 
      accgattcg tcggatgcc accgatagg cccctcgaa 
      cctccaaaa tcgaagtgt cacgttaat cctgacctt 
      tcattgtcc agttcaaat cctgatgat aatattctt 
      cttccctct catgataat aatgttgtt aattcgatg 
      caactttgt aaaacattc gggcaaaaa ggaaacggt 
      tcgggtcag tttaattca ccgcacgga ttttgtatc 
      gggtttaac gaggatatt gtcgttgct gatagtagc 
      aatcaccga gttcagatt tttgataag tatggccgt 
      tacaagtcc caatttggt ataaccggc cgtcatgat 
      ggctacttg tggcatcct cgtaaagtg gtctggtta 
      caaaaaaaa tgctgttac gtcgtttgt gatcgtggt 
      tacgagcga tcacgtatg caagttttc accaaagag 
      ggaaagttt tacaaaaaa atcaacgtt cattttatt 
      gatatcgtg gcaggcatt gctgtcact tccaatggg 
      gaaattgtt gttgtggac agtgtcaca cccacactt 
      ttcattgct gacgaaaat gatttcttg aggacactt 
      gattgctcg ccctttatt caggagcct tccgacatt 
      gccgttgtc gggaaggag ttttacatc tgcgacttt 
      aaaggtcac gcaattgtt gtgatggac gacgatggt 
      aattttttg cgcaagttt ggcggtact gaaaccacc 
      atgttcccg aatgggatt gatatttcg agtactggt 
      gatgtcata gtaggggat tcccacggc aattatttt 
      catgttgct gtttattcg aaacaaggg aattttctt 
      gctcaatat cgctgtcct gtcgttaaa gtttcacgc 
      tgttgcggt ttaaaaatg acctcagaa gggcgtttg 
      gtaacagtt gataaaaat tatcatcat gttattgtg 
      tttgaaagt ttgtttggg cctccagaa gcattttcg 
      tga
    mRNA sequence
    • >TC032733 RA
      tgattctta gaagctttg atggtcgat acatttact 
      ggtttaact agtttttaa cgtttttgt gtggtgttt 
      tatccttac aacatctta tttttatcg ttaattgca 
      ctcttgcac ctgcaatta tcatctaat tgcaaaaac 
      caagcgatc aagtcgacg aaacgcaaa aatgtcatt 
      cacaacagg atcatcccc tgactgttc cgactccca 
      cttaagcct aacgcccca gttctgcgt tcctgcttg 
      caccccatg tgtctcaga cacggaggc agagctcta 
      ctgtctcgt atgccagaa ttatctgtg caaacaatg 
      ctacttgta ccacgacat taacgacta tttaaacct 
      ccaccgtgt ggtttttat ttgcgaaat tgccaattt 
      ggacgaact gatccatct gatgtccga tccggacgt 
      ggtcttgaa cttgaccat tttcaactc tgactggat 
      ctacaagaa catgctgaa aaaacagat cactgagaa 
      caaacaagc gcttgaacg catgctggc aatgtccga 
      ttttttgcg caagcaatt gcacgtttt ggaaaatca 
      tcgctgtga aagtatgcg cttgatttg ttcgtcgca 
      tagacagtt tgaaactta tttgcggag tcagaaaga 
      ccagattgt tacctcttt gcaaaatga atacgacgg 
      ggtcaagat gtccattct tacgaaggc gcaaactgt 
      tgatcagac tgttaggga tttggtggt caagttgaa 
      taaaatgca gctgatgac ggaaaataa tggcgaaac 
      tgcgagtgg gattgatat ggagatggc ggctagaca 
      tgggctttt gctgaatgg tgattgtat ggataataa 
      tgtgttttt caacgactt tgcttcgta tttgccgga 
      tgatttcca ctcgaatgg caccgattc gtcggatgc 
      caccgatag gcccctcga acctccaaa atcgaagtg 
      tcacgttaa tcctgacct ttcattgtc cagttcaaa 
      tcctgatga taatattct tcttccctc tcatgataa 
      taatgttgt taattcgat gcaactttg taaaacatt 
      cgggcaaaa aggaaacgg ttcgggtca gtttaattc 
      accgcacgg attttgtat cgggtttaa cgaggatat 
      tgtcgttgc tgatagtag caatcaccg agttcagat 
      ttttgataa gtatggccg ttacaagtc ccaatttgg 
      tataaccgg ccgtcatga tggctactt gtggcatcc 
      tcgtaaagt ggtctggtt acaaaaaaa atgctgtta 
      cgtcgtttg tgatcgtgg ttacgagcg atcacgtat 
      gcaagtttt caccaaaga gggaaagtt ttacaaaaa 
      aatcaacgt tcattttat tgatatcgt ggcaggcat 
      tgctgtcac ttccaatgg ggaaattgt tgttgtgga 
      cagtgtcac acccacact tttcattgc tgacgaaaa 
      tgatttctt gaggacact tgattgctc gccctttat 
      tcaggagcc ttccgacat tgccgttgt cgggaagga 
      gttttacat ctgcgactt taaaggtca cgcaattgt 
      tgtgatgga cgacgatgg taatttttt gcgcaagtt 
      tggcggtac tgaaaccac catgttccc gaatgggat 
      tgatatttc gagtactgg tgatgtcat agtagggga 
      ttcccacgg caattattt tcatgttgc tgtttattc 
      gaaacaagg gaattttct tgctcaata tcgctgtcc 
      tgtcgttaa agtttcacg ctgttgcgg tttaaaaat 
      gacctcaga agggcgttt ggtaacagt tgataaaaa 
      ttatcatca tgttattgt gtttgaaag tttgtttgg 
      gcctccaga agcattttc gtgagctgt ttttttatg 
      tttaacttt tgttctttt attcatata ttttttaat 
      cgttttgtt cagatttaa cgacgtttt tctcgtcaa 
      aatttaggt ttaggataa gtttttcga tattgttta 
      cttgtgttt cgtatttct cgttcattt agctttagg 
      tttcgttgt gtttcgttc aaataaatt gttctttgc 
      att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY