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Gene information
General information
TC007710 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 58206..60159
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007710
    iBeetle screen iB_08072

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007710 RA
      MDRHRNPFT CYFCHEKIE NETEMGHTS VCGNVLIPC 
      PNKCGSYVP RMEMPLHKK ECLNRPSRS MTRLTTTTD 
      CLETYEDGI TSRTNNGSI RELIERLQH DFHKLSTRL 
      ANYEVASGS PKSKQELNK LQYQTQLLF EWKKAADFK 
      LDTLTQSLN SLEKSRNES EYHWMGLQQ KLMNFDKLQ 
      MDINLATES FLKEQNYNR QANLEFAKN LDEFKDLFE 
      QENVTFSHL WADQKRSIS DIQADINSL KHNFEEMKA 
      KQVSVVFDI KTISQIASE TAEKFELQE REFTKFRQQ 
      FEQMKLDLE ILENLASCD GRTSPGHLL WKIADFEDK 
      MSKAKDCNT VLKSPIFYT HNYGYKIRV LVYLNGLKK 
      WKDRYALIS IHVLKGDFD ALLKWPCHI EGTVTLRDQ 
      ENSAKNFTK HIVAKRQSG DEENEEPQE SSFSYIFIP 
      HATLTKSHH LKNDEIFLE VKIEQNRNL LTETTL
    CDS sequence
    • >TC007710 RA
      atggaccgg cacaggaac ccctttacg tgttatttt 
      tgtcacgaa aaaatcgaa aacgaaaca gaaatggga 
      catacatca gtttgtggc aacgtctta ataccttgt 
      cccaacaaa tgtggctct tatgtccct aggatggaa 
      atgccctta cacaagaaa gagtgtttg aatcgtccg 
      agccgatca atgacgcga ctaaccaca acaacagac 
      tgtttggaa acgtacgaa gatggaata acatctcga 
      acaaacaat ggttcgatt cgagaacta atcgagcgt 
      ttacagcac gattttcac aaactttcg accagatta 
      gccaattat gaagtggcc tctggttcc ccaaagtca 
      aaacaagaa ttgaacaaa ttacagtac caaactcag 
      ttacttttc gagtggaaa aaagcggct gattttaaa 
      ctagacacg ttaacacaa tcgttaaat tcgctagaa 
      aaaagtcga aacgaaagc gaatatcac tggatgggt 
      ctccagcaa aaactaatg aatttcgat aaactccaa 
      atggacata aacctagcc actgagtcg ttcctcaaa 
      gagcagaat tacaaccga caagctaat ctcgaattt 
      gcgaaaaat cttgacgag tttaaagat ttattcgaa 
      caggaaaat gtcactttt agtcacttg tgggccgat 
      caaaaacgc tccatttca gacatacaa gccgacatt 
      aattctctt aaacacaat ttcgaagaa atgaaagcc 
      aaacaggtg agtgtggtt ttcgatatc aaaactatc 
      agtcaaatc gcgtcagaa acggccgaa aaatttgaa 
      cttcaagag cgcgaattt acaaaattc agacaacaa 
      ttcgaacaa atgaagctg gatttggag attttggaa 
      aatttggct tcttgtgat ggaagaaca agtccggga 
      catctcctc tggaaaatt gccgatttt gaggataaa 
      atgtccaaa gcgaaggac tgtaacact gttctcaag 
      agtcccatt ttctacact cacaattac gggtacaaa 
      attagggtt ttagtttat ttaaacggc ttgaaaaaa 
      tggaaagac cgatacgct ctcatttca attcatgtt 
      ctcaagggc gattttgac gctcttttg aaatggccg 
      tgtcacatc gaagggact gtaactttg agggatcag 
      gaaaatagc gcgaaaaat ttcaccaaa catatcgta 
      gccaaaaga caatcagga gatgaggaa aatgaggag 
      ccgcaagaa tcatccttt tcctatatt tttatacca 
      catgcgacc ctaaccaaa tcgcatcac ttgaaaaat 
      gatgaaatt tttctcgaa gttaaaatc gaacaaaat 
      aggaattta ctaacggaa actacgttg taa
    mRNA sequence
    • >TC007710 RA
      acctggaaa aataatgga ccggcacag gaacccctt 
      tacgtgtta tttttgtca cgaaaaaat cgaaaacga 
      aacagaaat gggacatac atcagtttg tggcaacgt 
      cttaatacc ttgtcccaa caaatgtgg ctcttatgt 
      ccctaggat ggaaatgcc cttacacaa gaaagagtg 
      tttgaatcg tccgagccg atcaatgac gcgactaac 
      cacaacaac agactgttt ggaaacgta cgaagatgg 
      aataacatc tcgaacaaa caatggttc gattcgaga 
      actaatcga gcgtttaca gcacgattt tcacaaact 
      ttcgaccag attagccaa ttatgaagt ggcctctgg 
      ttccccaaa gtcaaaaca agaattgaa caaattaca 
      gtaccaaac tcagttact tttcgagtg gaaaaaagc 
      ggctgattt taaactaga cacgttaac acaatcgtt 
      aaattcgct agaaaaaag tcgaaacga aagcgaata 
      tcactggat gggtctcca gcaaaaact aatgaattt 
      cgataaact ccaaatgga cataaacct agccactga 
      gtcgttcct caaagagca gaattacaa ccgacaagc 
      taatctcga atttgcgaa aaatcttga cgagtttaa 
      agatttatt cgaacagga aaatgtcac ttttagtca 
      cttgtgggc cgatcaaaa acgctccat ttcagacat 
      acaagccga cattaattc tcttaaaca caatttcga 
      agaaatgaa agccaaaca ggtgagtgt ggttttcga 
      tatcaaaac tatcagtca aatcgcgtc agaaacggc 
      cgaaaaatt tgaacttca agagcgcga atttacaaa 
      attcagaca acaattcga acaaatgaa gctggattt 
      ggagatttt ggaaaattt ggcttcttg tgatggaag 
      aacaagtcc gggacatct cctctggaa aattgccga 
      ttttgagga taaaatgtc caaagcgaa ggactgtaa 
      cactgttct caagagtcc cattttcta cactcacaa 
      ttacgggta caaaattag ggttttagt ttatttaaa 
      cggcttgaa aaaatggaa agaccgata cgctctcat 
      ttcaattca tgttctcaa gggcgattt tgacgctct 
      tttgaaatg gccgtgtca catcgaagg gactgtaac 
      tttgaggga tcaggaaaa tagcgcgaa aaatttcac 
      caaacatat cgtagccaa aagacaatc aggagatga 
      ggaaaatga ggagccgca agaatcatc cttttccta 
      tatttttat accacatgc gaccctaac caaatcgca 
      tcacttgaa aaatgatga aatttttct cgaagttaa 
      aatcgaaca aaataggaa tttactaac ggaaactac 
      gttgtaata atatttttt gtattaata aagaaataa 
      aaatgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY