Status
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Gene information
General information
TC004359 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7139000..7142541
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004359
    iBeetle screen iB_08070

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004359 RA
      MNAEIEENI RKNVPWSQL PAHIKQILN NSVKDYERY 
      VVSFSIKNQ LRYRGNLVR HIVRDEKRY YERVVAYSK 
      ERLMLFPYH LADMIVKGL RITPFNYYI SVVEKLMQA 
      EKSYDTLPN FTAADCLRL LGIGRNEYI ELMNKSRSN 
      RGRLFGKKN VRGLLPPVP KDIHIEPWW RVEVGLVLE 
      EDVKLVNEE ELAVIDRLI DLGSQTAGE LNYHVVLSL 
      YKKGLIYLD VPITAVDCI QVPPLQGFV MNRVLGDYF 
      ETLLYKIFV SIDEHTTVG ELAGVLQVD TESVKQAVS 
      LYCRLQFAR KLHPETDKE RVKRHPTWH LIPTQEIRN 
      VEVTPLTLS LSDNLQEQP ESPSTLTRK GHRVAFLFD 
      SALTAFLMM GNLSPGLKK HAVTMFEVG KLCDESLDS 
      FLTELEKVS VLDAEGEGE ARRFFDHAV ILRSTILAL 
      RKLPGSGLD LVRLESLHS LDEATCSRL LQKKYNLLV 
      SMAPLSREV RPVVSLNPP HLGPAVPEV NSLWFKMFL 
      YHVSGYGPP SLLLTKGTE IKQLPRMFL GYSRLLVTT 
      WLHEPAVIT VSNILHVNA AAQFSPVLI QAFGVHEPA 
      QTHIIPFPF RPDTPSKSL GEVPQDLQF RNHPSVKYL 
      SNLIDLEHN CGYITFANI GVLDFGCQH KEMTVKLGK 
      KQPIASTKG KAEPLSITN ENFSFTKEL SSPDESHFA 
      ITPPTSSSP ANGFTSKEC SELLRQELD ELEQKMKHS 
      VSIDGLLSP IDENINMFS LKSEEMKKP DENRGEVWT 
      LLDCHFGIP LFDVNANTK ICDMIVSGG LVETNSLSN 
      LVESSRKLG SALLDFISQ CQYYPGENM EIMKRGRLV 
      PLPRYNLVF DNGKVSEWT GK
    CDS sequence
    • >TC004359 RA
      atgaatgcc gaaatcgag gaaaatatc cgtaaaaac 
      gtgccatgg tctcaactc cccgcacat atcaaacag 
      attttaaac aattcggtc aaagactac gaacgctac 
      gtggtctcg ttcagtatt aaaaaccag ttgcgctat 
      cgcggaaat ttagtacga cacatcgtc cgagatgag 
      aaacgttac tacgaacgt gtggtggct tacagtaaa 
      gagagactc atgttattc ccctaccac ttggccgat 
      atgattgtc aaaggtttg cggataacg ccgttcaat 
      tactacatt tcagtggtt gagaaattg atgcaagct 
      gagaaaagt tacgatact cttccgaac tttacggca 
      gcagattgt ttgaggtta ttggggatt ggcagaaat 
      gaatacatc gaattgatg aataagtcg aggtcgaat 
      agagggcgc ttgtttggg aaaaaaaat gtaaggggg 
      ctcctacca cccgtcccc aaagacata cacattgag 
      ccgtggtgg agggtcgaa gttggcctt gtattggaa 
      gaagacgtc aaattggtg aatgaggaa gagttggcg 
      gtgattgac cgtttaatt gatttgggg tcacaaact 
      gcgggcgaa ttaaactat catgttgtt ttgagtctt 
      tacaagaaa ggtttgatt tatttggac gttcctata 
      accgcagtt gattgtata caagtgccg cctttgcaa 
      ggttttgta atgaatcgt gtccttggt gattatttt 
      gaaacactg ttgtataaa atattcgtg tcaattgac 
      gaacatacg acagttggc gaacttgcc ggggtttta 
      caggttgat actgagtcg gtaaaacag gcagtttca 
      ctctattgt cgcttacaa tttgcacga aaattgcat 
      cccgaaact gacaaagaa cgtgtcaaa agacaccca 
      acgtggcat ttaatacca acacaagaa attagaaac 
      gttgaagtg acaccactg accttgagt ctcagtgat 
      aatctccaa gagcaaccc gaaagtcct tcgaccttg 
      acccgaaag gggcatcga gtggctttt ctcttcgat 
      tcggccttg actgctttc ctaatgatg ggaaattta 
      tcaccgggc ttaaaaaaa cacgccgtg accatgttt 
      gaagttggt aaattgtgc gacgaaagt cttgatagt 
      tttttaacc gaacttgag aaagtatca gttcttgat 
      gctgaaggt gaaggtgaa gcgcgacgt ttcttcgat 
      catgctgtc attctcaga tccacgatt ttggctttg 
      agaaaactc cccggctct ggtttggat ttggtacga 
      cttgaaagt ctccattca ttagacgag gctacttgt 
      agccgactt ttacagaaa aaatacaat ctgttggta 
      tcaatggca ccgttaagc cgggaagta cggccagtt 
      gtgagtcta aatccgccc catttaggg ccagcagtc 
      cccgaagtg aattcgttg tggtttaaa atgtttttg 
      tatcacgtg agcggttat ggaccgcca tctttgtta 
      ctaaccaaa ggaactgaa atcaagcaa cttccgcgt 
      atgttcctc gggtattcc cggcttttg gtaaccacg 
      tggttacac gaaccggcg gttataacc gtttcgaat 
      attttacat gtgaatgcg gctgcgcaa ttttccccc 
      gtattgatt caggctttt ggggttcat gaaccggct 
      cagactcac ataattccg tttcccttt cgtcccgac 
      acaccttcc aaaagttta ggggaagtc cctcaagac 
      ttacaattc cggaatcat ccctcagtt aaatatctc 
      tcgaattta attgacctt gaacacaat tgtggttac 
      atcacgttt gccaacatt ggagtatta gatttcggc 
      tgtcaacat aaagaaatg acagtcaag cttggcaaa 
      aaacaacca atcgcgtcc accaaaggc aaggccgag 
      cctttatca atcaccaat gagaatttt agtttcact 
      aaagagttg agttcaccc gatgaatca cattttgcc 
      ataactccg cccacttcg tcatcacca gccaatggt 
      ttcacaagt aaggaatgt tcggaactc ctacgtcaa 
      gaattggac gaattagaa caaaagatg aaacacagc 
      gtttcaatt gatggtctt ctatcacca atcgacgaa 
      aacatcaac atgttttcg ctaaaaagc gaagaaatg 
      aaaaaaccc gacgaaaat cgaggcgag gtttggact 
      ttgttagat tgccatttt gggattccc ctgtttgat 
      gttaacgcc aatactaaa atttgtgat atgatagtg 
      agtggcggt ttggttgaa actaacagt ttgtcgaat 
      ttagtcgaa tcgagtcgg aaattaggt tcggcactt 
      ttagatttt atttcgcaa tgtcagtac tatccgggt 
      gaaaatatg gaaattatg aaacgtgga cggttggta 
      ccattgcca cgttataac ctcgtcttc gataatggt 
      aaagtgagc gaatggacg ggaaaataa
    mRNA sequence
    • >TC004359 RA
      cattacatt gatttattt aaaaaaaaa acacgcttt 
      cttgcccaa ttcatgcca aaatcgcgt aaaaacagc 
      aaaattgtg caattgtga cacctgtca ttactgact 
      gtcacttgt caaatgggt ttatcaaca acaaattaa 
      cccatttac cagcatgaa tgccgaaat cgaggaaaa 
      tatccgtaa aaacgtgcc atggtctca actccccgc 
      acatatcaa acagatttt aaacaattc ggtcaaaga 
      ctacgaacg ctacgtggt ctcgttcag tattaaaaa 
      ccagttgcg ctatcgcgg aaatttagt acgacacat 
      cgtccgaga tgagaaacg ttactacga acgtgtggt 
      ggcttacag taaagagag actcatgtt attccccta 
      ccacttggc cgatatgat tgtcaaagg tttgcggat 
      aacgccgtt caattacta catttcagt ggttgagaa 
      attgatgca agctgagaa aagttacga tactcttcc 
      gaactttac ggcagcaga ttgtttgag gttattggg 
      gattggcag aaatgaata catcgaatt gatgaataa 
      gtcgaggtc gaatagagg gcgcttgtt tgggaaaaa 
      aaatgtaag ggggctcct accacccgt ccccaaaga 
      catacacat tgagccgtg gtggagggt cgaagttgg 
      ccttgtatt ggaagaaga cgtcaaatt ggtgaatga 
      ggaagagtt ggcggtgat tgaccgttt aattgattt 
      ggggtcaca aactgcggg cgaattaaa ctatcatgt 
      tgttttgag tctttacaa gaaaggttt gatttattt 
      ggacgttcc tataaccgc agttgattg tatacaagt 
      gccgccttt gcaaggttt tgtaatgaa tcgtgtcct 
      tggtgatta ttttgaaac actgttgta taaaatatt 
      cgtgtcaat tgacgaaca tacgacagt tggcgaact 
      tgccggggt tttacaggt tgatactga gtcggtaaa 
      acaggcagt ttcactcta ttgtcgctt acaatttgc 
      acgaaaatt gcatcccga aactgacaa agaacgtgt 
      caaaagaca cccaacgtg gcatttaat accaacaca 
      agaaattag aaacgttga agtgacacc actgacctt 
      gagtctcag tgataatct ccaagagca acccgaaag 
      tccttcgac cttgacccg aaaggggca tcgagtggc 
      ttttctctt cgattcggc cttgactgc tttcctaat 
      gatgggaaa tttatcacc gggcttaaa aaaacacgc 
      cgtgaccat gtttgaagt tggtaaatt gtgcgacga 
      aagtcttga tagtttttt aaccgaact tgagaaagt 
      atcagttct tgatgctga aggtgaagg tgaagcgcg 
      acgtttctt cgatcatgc tgtcattct cagatccac 
      gattttggc tttgagaaa actccccgg ctctggttt 
      ggatttggt acgacttga aagtctcca ttcattaga 
      cgaggctac ttgtagccg acttttaca gaaaaaata 
      caatctgtt ggtatcaat ggcaccgtt aagccggga 
      agtacggcc agttgtgag tctaaatcc gccccattt 
      agggccagc agtccccga agtgaattc gttgtggtt 
      taaaatgtt tttgtatca cgtgagcgg ttatggacc 
      gccatcttt gttactaac caaaggaac tgaaatcaa 
      gcaacttcc gcgtatgtt cctcgggta ttcccggct 
      tttggtaac cacgtggtt acacgaacc ggcggttat 
      aaccgtttc gaatatttt acatgtgaa tgcggctgc 
      gcaattttc ccccgtatt gattcaggc ttttggggt 
      tcatgaacc ggctcagac tcacataat tccgtttcc 
      ctttcgtcc cgacacacc ttccaaaag tttagggga 
      agtccctca agacttaca attccggaa tcatccctc 
      agttaaata tctctcgaa tttaattga ccttgaaca 
      caattgtgg ttacatcac gtttgccaa cattggagt 
      attagattt cggctgtca acataaaga aatgacagt 
      caagcttgg caaaaaaca accaatcgc gtccaccaa 
      aggcaaggc cgagccttt atcaatcac caatgagaa 
      ttttagttt cactaaaga gttgagttc acccgatga 
      atcacattt tgccataac tccgcccac ttcgtcatc 
      accagccaa tggtttcac aagtaagga atgttcgga 
      actcctacg tcaagaatt ggacgaatt agaacaaaa 
      gatgaaaca cagcgtttc aattgatgg tcttctatc 
      accaatcga cgaaaacat caacatgtt ttcgctaaa 
      aagcgaaga aatgaaaaa acccgacga aaatcgagg 
      cgaggtttg gactttgtt agattgcca ttttgggat 
      tcccctgtt tgatgttaa cgccaatac taaaatttg 
      tgatatgat agtgagtgg cggtttggt tgaaactaa 
      cagtttgtc gaatttagt cgaatcgag tcggaaatt 
      aggttcggc acttttaga ttttatttc gcaatgtca 
      gtactatcc gggtgaaaa tatggaaat tatgaaacg 
      tggacggtt ggtaccatt gccacgtta taacctcgt 
      cttcgataa tggtaaagt gagcgaatg gacgggaaa 
      ataaataaa taagcaata ttttgtaca aattttgtg 
      ataagttgg agacttaat tttgtaaat actttgcat 
      ttattctat ggcagggtt aggtttcat tttccggac 
      caaaacatt tgattacat ttccaaacg tactttact 
      ttaagtatt aattatttt attaaaact agaaataaa 
      cacgtattg aaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY