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Gene information
General information
TC004365 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7087866..7093411
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004365
    iBeetle screen iB_08063

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004365 RA
      MAPILNLKL TTSHKFCPD SPLLNALKS CNDDSLYKK 
      LIDEILDTY HHHQDFGAR NVIIEALIN TNGRKNLLK 
      YMYKQLNVR QIGQSSFFE HIASSVMNE CRENFDNFV 
      FSLTLCEKV VQFFACFIQ NFPDDFLEK TFLFYFYSL 
      EALQSHGND CYVSMVLKQ LAVTLPHAN MKQLSNYAF 
      EMVVMTFEI LQNCEFKFE TILDSFVVF SYFSTLLPA 
      KETIFLKHR NYPILEFID NEKNDFCLI LQSKNFPNL 
      FKTGRLKAI LYLTISNTV PRDVLLNVK VAKKPLYES 
      LYTNLIDLG MSLTNFPDP IVPITRTLD SLSVLITRS 
      SNFSELFIK SFDFIWTHL DHFVDQVTQ NTKSLFEKL 
      TIEAQKRGE KLALEKLIA DLDSLPTFG KVRLVAYHV 
      TSHVIDSYL YDSNFRNKL LMVAAATSD QIASLAYSN 
      LMLQESCDE KIVQVWLKP VLGVLRMKQ TNVLEDIVT 
      LAVRKKPTI FPILFPNLV TTTVECSIV LKCVRLARL 
      NGDKFVSDV DSVDTWRGL IPEKQMELF MCHHDDEIR 
      IRVLQAVAE CQKSTEIFT EFELTFLIR YFRYNVSVT 
      SANIRNRFV TYFKKILRR FDVTWTKSP NSEYDNFLR 
      HLLEILIIN LSPDSNFSR RCISMQLLL LIRNFYDWR 
      NLFQADDIR NLKYLILDG YESNKSLAV EILKSFPVE 
      KFDLSDNFA EKLVTMLTD IRPNQSLSA CYYFMLVQA 
      EINVIAPLF DSDTNLNAE TPIYGIFQL IRYNPDAFT 
      YTFKQYCQL CLKYATAVL PIVANLAPE GYLPEETDI 
      TDSDQSSKS QKILVNSWK TMKEIMLLM ADLVKKNIQ 
      NEEENSEFF LRKIGDFFL DIFIQAKHR GVFEQAHVA 
      FNSICQSFL TSKNRNLNE LPSRWLTES VSLCTGETI 
      DDRLSPTRR SAGLPFLIL SLLPNLAES NIEFLRSTL 
      DSLLTISGD SPIHCLNVL RFVFKDAKL GENIVTYVD 
      RGAILALKN YKSRTWGVR NSATLLWSA LVRRIFGVK 
      ETKMALNVF YARYPQLFE FLLHELQHE CHKSESLVL 
      EPILMVLTQ LSPGCNETI NTQVLKYLC YIDVCMKSS 
      CLRTRDLAA RASIALIGE HSFQMCLDE SFGRISGNL 
      TMNERHGLL LQILHIVQS KDFDKLPIT TYLEQSLGL 
      LTRGESRVH HPLCMTLYM DIVFMLLSR SRDYKNLDL 
      LKKIVELLS EMRTEGAHA EWSRFQLLL FIVLNKFKR 
      CDNTYHVIA AMFVKYLTE NTPIRQKCL HLLIYLNQE 
      VSCDRFKIH PLIVAGNVE ICTQIKLLV HSFDSHTKK 
      QLLASLHSP LLRFFATEL QTKRNDLVL VLVLLDFYP 
      CFFETDRRL DTLIAMATN DEVTSGVIS VVKTILKNW 
      QLEVGGSLV KLLQESAAP AAPEWLRLA VAQCLVDIW 
      NNCKVPPLE KYNLANIVL VLLEDDEAL VRETASQVG 
      NTVSCSERS RELFLSSVV WELPVDYAI NFLVSWSLR 
      HLPEVSRDV SDVYERGEL NTWAENIPV AYAASCVLH 
      RLVWDHDGS VLIEERALL VTRVVLNAL IKLPSLMVD 
      SGVKKTVIC ALRRILVFI EGCTSDFNR DFKLFFGTK 
      ILTFLRNRV KNDDYATVK CFFTYLYNP VLELHFRFP
    CDS sequence
    • >TC004365 RA
      atggccccc attcttaat ttgaaattg accacaagt 
      cacaaattt tgccccgac agccccctt ctgaatgcc 
      ctaaaatca tgcaatgac gattccctc tataaaaaa 
      ctgatcgac gaaatcctt gacacctac caccatcat 
      caggacttt ggcgcccga aacgtcata atcgaagcg 
      ttaattaac acaaacggg cgcaaaaat ttgctcaaa 
      tacatgtac aagcagtta aatgtgcgc caaattggc 
      caaagtagt tttttcgaa cacattgca tcaagtgtg 
      atgaatgaa tgtagagaa aatttcgat aattttgtt 
      ttcagttta acgttgtgc gagaaagtc gtgcagttt 
      tttgcgtgt tttattcaa aattttccc gatgatttt 
      ctagaaaag acgtttttg ttttatttc tacagtctg 
      gaggcgctt caatcccat ggtaacgat tgttacgtg 
      agtatggtt ttgaaacag ttggctgtg acgttacca 
      catgccaat atgaaacaa ttgagtaac tatgccttt 
      gaaatggtt gttatgact tttgagatt ttgcaaaat 
      tgcgaattt aaatttgag acgattttg gacagtttc 
      gtggttttt tcgtacttc tcaaccctt ttgcctgca 
      aaagaaaca atttttttg aaacaccga aattacccc 
      attttggag tttatagat aacgaaaaa aatgatttt 
      tgtttgatt ctccaaagt aaaaatttt ccgaattta 
      ttcaaaact ggtcgtttg aaagcaatt ctttacttg 
      actatttca aacactgta ccacgtgac gtccttttg 
      aacgtaaaa gtggccaaa aaaccgctt tacgaatca 
      ctttataca aacttaatt gatttaggg atgagtttg 
      acaaacttc cccgaccca attgtcccc ataacacga 
      actttggac tcactttcg gtcttaatt acacgttcg 
      tcgaacttt tcggaactt tttatcaaa agtttcgac 
      tttatttgg acacatttg gaccacttc gtcgatcag 
      gtgacccaa aacacgaaa tcactcttt gaaaaattg 
      acaattgaa gcacaaaaa cgtggcgaa aagttggcg 
      ttggaaaaa ctcatcgcc gacttggat tcgttaccg 
      actttcggt aaagtacga cttgttgcg tatcacgtg 
      accagtcac gtaatcgat tcgtatttg tatgattcg 
      aattttcgc aacaaactt ttaatggtt gccgcggca 
      acatcggat cagattgca agtttagcc tattccaat 
      ctaatgcta caggagagt tgtgacgaa aaaatagtg 
      caggtttgg ttaaaacca gttttgggt gttttacga 
      atgaaacaa acgaacgtt ttggaagat attgttacg 
      ttggctgtg cgtaaaaaa ccaacaatt ttcccgatt 
      ttgtttcca aatctagtg acaacgacg gttgagtgt 
      tcaattgtt ttaaagtgt gttcggttg gcacgtttg 
      aacggtgat aaattcgta tcagacgtt gacagtgtt 
      gacacctgg cgtgggtta atccccgaa aaacaaatg 
      gaattgttt atgtgtcac catgacgac gagattcgc 
      atcagagtg ttgcaagcc gtagccgag tgtcagaaa 
      agtactgaa atttttacc gaattcgaa ttgacgttt 
      ctgattcgg tactttcgg tacaacgtt tcagtcaca 
      agtgccaac atcaggaat cggttcgtg acgtatttt 
      aagaagatt ttgcgccga tttgacgtc acttggacc 
      aaaagtccg aattcggaa tatgataat tttctacgt 
      cacctattg gagatttta ataattaat ttatcgccc 
      gattcgaat ttttcccgc cgttgtatt tctatgcaa 
      ttgctactt ttaattcgt aatttttac gattggaga 
      aatttgttt caggccgat gatattcgg aatttaaaa 
      tacctaatt cttgacggt tatgaatcg aataaaagt 
      ttagccgtt gaaattttg aagtcattt ccggtcgaa 
      aaattcgac ctttcggac aatttcgcc gaaaagttg 
      gtaaccatg cttacggat attcgtccg aatcaaagc 
      ctttcggct tgttactac ttcatgttg gtacaagcg 
      gaaatcaat gtcattgca cctttattt gacagtgat 
      accaactta aatgccgaa accccgatt tatggaatt 
      ttccaactt attcgctac aatccggac gctttcacc 
      tacacattc aaacagtac tgccaactg tgcttaaaa 
      tacgccact gcagtgtta ccaattgtc gccaacttg 
      gcaccggaa ggttacctt cctgaagaa accgacatt 
      accgactcg gatcaatcg tcaaaatcg caaaaaatt 
      cttgtcaat tcgtggaaa actatgaaa gaaatcatg 
      ctgttgatg gcagatctt gtcaaaaaa aacatacaa 
      aatgaggag gaaaattcg gaatttttt ctacgaaaa 
      atcggcgat tttttctta gatattttc attcaagcc 
      aaacatcga ggcgttttc gagcaagct catgtcgct 
      ttcaacagc atttgtcaa agttttctg acttcaaaa 
      aaccgcaat ttgaacgaa ttgccatca aggtggctt 
      accgagtcc gtttctctt tgcactggt gaaacaatc 
      gacgatcgt ttatcaccg acacgtcgc agtgccggt 
      cttcctttt ctaattctg agtctcctt ccgaacctt 
      gccgaatca aacatcgag tttctccga agcactctc 
      gattcgctt ttaacaata tcgggtgat tcccctatc 
      cattgtctc aatgtttta cgtttcgta ttcaaagat 
      gcgaaattg ggtgaaaat atcgtcaca tatgtggat 
      cgtggtgca attttggcg ttaaaaaat tacaaaagc 
      cgtacatgg ggagtacga aattctgcc acgcttttg 
      tggtcggcg ctagtgagg cgtatcttt ggtgtgaag 
      gaaactaag atggctttg aacgtgttt tatgcccgt 
      tatccgcaa ttgtttgag tttttattg cacgagcta 
      caacacgag tgccataaa agtgaaagt ctagttttg 
      gaaccaatt ttaatggtt ttaacccaa ctttcaccg 
      ggttgtaat gagacgatt aatacacaa gtgttgaaa 
      tatttgtgt tatattgat gtgtgtatg aaaagttcg 
      tgtttacgc acgagggac ttggccgcg agggcctca 
      attgcactg attggtgaa cattcgttt caaatgtgt 
      ttggatgaa agctttgga cgaatttcg gggaatttg 
      acgatgaat gaacgtcac ggattgtta ttacaaatt 
      ttacatatt gtacaaagc aaagatttc gataaatta 
      ccaataaca acgtatctt gagcaaagt ttgggttta 
      ttgacacga ggcgaaagc cgagtgcat cacccctta 
      tgtatgaca ttgtacatg gatattgtt tttatgcta 
      ctatcaaga agtcgtgat tacaaaaat ttggattta 
      ttaaaaaaa atcgtagag ttgttatca gagatgcgt 
      acagagggc gcacatgcc gaatggagc cgttttcaa 
      ttgttactt ttcatcgtt ttaaataaa ttcaaacgt 
      tgtgataac acatatcac gtgatcgcc gccatgttt 
      gtcaaatat ttgacagaa aacacgcca atacgtcaa 
      aagtgtctc catttattg atttatctg aatcaagaa 
      gtgagctgt gaccggttt aaaatacac cccttaata 
      gtggccggt aatgtagaa atctgtact caaatcaaa 
      cttttggta cattcgttt gattcacac acgaaaaaa 
      caattgcta gctagtcta cattcaccc ttgttgcgt 
      tttttcgcc actgaacta caaacgaaa cgcaacgat 
      ttggttttg gtattagtt ttattggac ttttatccg 
      tgttttttt gagacggac cgcagactg gacacgtta 
      attgccatg gcaacgaat gacgaagtc acatctggt 
      gttatatcc gttgtgaaa acaatatta aaaaattgg 
      caactagaa gtgggtggg agtttggtt aaattatta 
      caggagtcc gcagccccg gcagccccc gagtggctt 
      cgcctggct gtggcccag tgtctcgtt gacatatgg 
      aacaactgt aaagtgccc cctttggaa aaatacaat 
      ttagccaac attgtcctg gttttattg gaagatgat 
      gaggcactt gttcgcgaa acagcgagt caagttggt 
      aatacagtt tcctgttcg gagcgttca cgcgaactg 
      tttctctca tcagttgtt tgggaatta ccggtcgat 
      tatgccata aattttctc gtttcgtgg tcgttgcgc 
      cacctaccc gaagtgtca cgtgacgtt tctgacgtt 
      tatgaaagg ggtgagttg aacacatgg gctgagaat 
      attccagtg gcgtacgcg gccagttgt gtgcttcat 
      aggttggtt tgggatcat gatgggtct gttttaatc 
      gaagagagg gcgctgttg gtcacacgg gttgtgctg 
      aacgctcta atcaagtta ccatcgctt atggtggat 
      tcaggggtg aagaaaact gtgatttgt gcgttgagg 
      cggatttta gtgtttatt gaggggtgt acaagtgat 
      tttaatagg gattttaag ttgtttttt ggtacgaaa 
      atattgacg tttttgcga aatcgggtc aagaatgat 
      gactatgcc actgttaag tgttttttt acttatttg 
      tataatccg gtcctagaa ttgcatttc aggtttccg 
      taa
    mRNA sequence
    • >TC004365 RA
      ttacttaaa aaaaaatgc gttaacaca tttaacgtg 
      ttttatgtg atattttgt ggaaaatca ttaaataac 
      actgcaagt cgttcgtta tggccccca ttcttaatt 
      tgaaattga ccacaagtc acaaatttt gccccgaca 
      gcccccttc tgaatgccc taaaatcat gcaatgacg 
      attccctct ataaaaaac tgatcgacg aaatccttg 
      acacctacc accatcatc aggactttg gcgcccgaa 
      acgtcataa tcgaagcgt taattaaca caaacgggc 
      gcaaaaatt tgctcaaat acatgtaca agcagttaa 
      atgtgcgcc aaattggcc aaagtagtt ttttcgaac 
      acattgcat caagtgtga tgaatgaat gtagagaaa 
      atttcgata attttgttt tcagtttaa cgttgtgcg 
      agaaagtcg tgcagtttt ttgcgtgtt ttattcaaa 
      attttcccg atgattttc tagaaaaga cgtttttgt 
      tttatttct acagtctgg aggcgcttc aatcccatg 
      gtaacgatt gttacgtga gtatggttt tgaaacagt 
      tggctgtga cgttaccac atgccaata tgaaacaat 
      tgagtaact atgcctttg aaatggttg ttatgactt 
      ttgagattt tgcaaaatt gcgaattta aatttgaga 
      cgattttgg acagtttcg tggtttttt cgtacttct 
      caacccttt tgcctgcaa aagaaacaa tttttttga 
      aacaccgaa attacccca ttttggagt ttatagata 
      acgaaaaaa atgattttt gtttgattc tccaaagta 
      aaaattttc cgaatttat tcaaaactg gtcgtttga 
      aagcaattc tttacttga ctatttcaa acactgtac 
      cacgtgacg tccttttga acgtaaaag tggccaaaa 
      aaccgcttt acgaatcac tttatacaa acttaattg 
      atttaggga tgagtttga caaacttcc ccgacccaa 
      ttgtcccca taacacgaa ctttggact cactttcgg 
      tcttaatta cacgttcgt cgaactttt cggaacttt 
      ttatcaaaa gtttcgact ttatttgga cacatttgg 
      accacttcg tcgatcagg tgacccaaa acacgaaat 
      cactctttg aaaaattga caattgaag cacaaaaac 
      gtggcgaaa agttggcgt tggaaaaac tcatcgccg 
      acttggatt cgttaccga ctttcggta aagtacgac 
      ttgttgcgt atcacgtga ccagtcacg taatcgatt 
      cgtatttgt atgattcga attttcgca acaaacttt 
      taatggttg ccgcggcaa catcggatc agattgcaa 
      gtttagcct attccaatc taatgctac aggagagtt 
      gtgacgaaa aaatagtgc aggtttggt taaaaccag 
      ttttgggtg ttttacgaa tgaaacaaa cgaacgttt 
      tggaagata ttgttacgt tggctgtgc gtaaaaaac 
      caacaattt tcccgattt tgtttccaa atctagtga 
      caacgacgg ttgagtgtt caattgttt taaagtgtg 
      ttcggttgg cacgtttga acggtgata aattcgtat 
      cagacgttg acagtgttg acacctggc gtgggttaa 
      tccccgaaa aacaaatgg aattgttta tgtgtcacc 
      atgacgacg agattcgca tcagagtgt tgcaagccg 
      tagccgagt gtcagaaaa gtactgaaa tttttaccg 
      aattcgaat tgacgtttc tgattcggt actttcggt 
      acaacgttt cagtcacaa gtgccaaca tcaggaatc 
      ggttcgtga cgtatttta agaagattt tgcgccgat 
      ttgacgtca cttggacca aaagtccga attcggaat 
      atgataatt ttctacgtc acctattgg agattttaa 
      taattaatt tatcgcccg attcgaatt tttcccgcc 
      gttgtattt ctatgcaat tgctacttt taattcgta 
      atttttacg attggagaa atttgtttc aggccgatg 
      atattcgga atttaaaat acctaattc ttgacggtt 
      atgaatcga ataaaagtt tagccgttg aaattttga 
      agtcatttc cggtcgaaa aattcgacc tttcggaca 
      atttcgccg aaaagttgg taaccatgc ttacggata 
      ttcgtccga atcaaagcc tttcggctt gttactact 
      tcatgttgg tacaagcgg aaatcaatg tcattgcac 
      ctttatttg acagtgata ccaacttaa atgccgaaa 
      ccccgattt atggaattt tccaactta ttcgctaca 
      atccggacg ctttcacct acacattca aacagtact 
      gccaactgt gcttaaaat acgccactg cagtgttac 
      caattgtcg ccaacttgg caccggaag gttaccttc 
      ctgaagaaa ccgacatta ccgactcgg atcaatcgt 
      caaaatcgc aaaaaattc ttgtcaatt cgtggaaaa 
      ctatgaaag aaatcatgc tgttgatgg cagatcttg 
      tcaaaaaaa acatacaaa atgaggagg aaaattcgg 
      aattttttc tacgaaaaa tcggcgatt ttttcttag 
      atattttca ttcaagcca aacatcgag gcgttttcg 
      agcaagctc atgtcgctt tcaacagca tttgtcaaa 
      gttttctga cttcaaaaa accgcaatt tgaacgaat 
      tgccatcaa ggtggctta ccgagtccg tttctcttt 
      gcactggtg aaacaatcg acgatcgtt tatcaccga 
      cacgtcgca gtgccggtc ttccttttc taattctga 
      gtctccttc cgaaccttg ccgaatcaa acatcgagt 
      ttctccgaa gcactctcg attcgcttt taacaatat 
      cgggtgatt cccctatcc attgtctca atgttttac 
      gtttcgtat tcaaagatg cgaaattgg gtgaaaata 
      tcgtcacat atgtggatc gtggtgcaa ttttggcgt 
      taaaaaatt acaaaagcc gtacatggg gagtacgaa 
      attctgcca cgcttttgt ggtcggcgc tagtgaggc 
      gtatctttg gtgtgaagg aaactaaga tggctttga 
      acgtgtttt atgcccgtt atccgcaat tgtttgagt 
      ttttattgc acgagctac aacacgagt gccataaaa 
      gtgaaagtc tagttttgg aaccaattt taatggttt 
      taacccaac tttcaccgg gttgtaatg agacgatta 
      atacacaag tgttgaaat atttgtgtt atattgatg 
      tgtgtatga aaagttcgt gtttacgca cgagggact 
      tggccgcga gggcctcaa ttgcactga ttggtgaac 
      attcgtttc aaatgtgtt tggatgaaa gctttggac 
      gaatttcgg ggaatttga cgatgaatg aacgtcacg 
      gattgttat tacaaattt tacatattg tacaaagca 
      aagatttcg ataaattac caataacaa cgtatcttg 
      agcaaagtt tgggtttat tgacacgag gcgaaagcc 
      gagtgcatc accccttat gtatgacat tgtacatgg 
      atattgttt ttatgctac tatcaagaa gtcgtgatt 
      acaaaaatt tggatttat taaaaaaaa tcgtagagt 
      tgttatcag agatgcgta cagagggcg cacatgccg 
      aatggagcc gttttcaat tgttacttt tcatcgttt 
      taaataaat tcaaacgtt gtgataaca catatcacg 
      tgatcgccg ccatgtttg tcaaatatt tgacagaaa 
      acacgccaa tacgtcaaa agtgtctcc atttattga 
      tttatctga atcaagaag tgagctgtg accggttta 
      aaatacacc ccttaatag tggccggta atgtagaaa 
      tctgtactc aaatcaaac ttttggtac attcgtttg 
      attcacaca cgaaaaaac aattgctag ctagtctac 
      attcaccct tgttgcgtt ttttcgcca ctgaactac 
      aaacgaaac gcaacgatt tggttttgg tattagttt 
      tattggact tttatccgt gtttttttg agacggacc 
      gcagactgg acacgttaa ttgccatgg caacgaatg 
      acgaagtca catctggtg ttatatccg ttgtgaaaa 
      caatattaa aaaattggc aactagaag tgggtggga 
      gtttggtta aattattac aggagtccg cagccccgg 
      cagcccccg agtggcttc gcctggctg tggcccagt 
      gtctcgttg acatatgga acaactgta aagtgcccc 
      ctttggaaa aatacaatt tagccaaca ttgtcctgg 
      ttttattgg aagatgatg aggcacttg ttcgcgaaa 
      cagcgagtc aagttggta atacagttt cctgttcgg 
      agcgttcac gcgaactgt ttctctcat cagttgttt 
      gggaattac cggtcgatt atgccataa attttctcg 
      tttcgtggt cgttgcgcc acctacccg aagtgtcac 
      gtgacgttt ctgacgttt atgaaaggg gtgagttga 
      acacatggg ctgagaata ttccagtgg cgtacgcgg 
      ccagttgtg tgcttcata ggttggttt gggatcatg 
      atgggtctg ttttaatcg aagagaggg cgctgttgg 
      tcacacggg ttgtgctga acgctctaa tcaagttac 
      catcgctta tggtggatt caggggtga agaaaactg 
      tgatttgtg cgttgaggc ggattttag tgtttattg 
      aggggtgta caagtgatt ttaataggg attttaagt 
      tgttttttg gtacgaaaa tattgacgt ttttgcgaa 
      atcgggtca agaatgatg actatgcca ctgttaagt 
      gttttttta cttatttgt ataatccgg tcctagaat 
      tgcatttca ggtttccgt aattaatca ataaaaatt 
      taaattgtg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY