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Gene information
General information
TC016391 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 30065596..30095106
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016391
    iBeetle screen iB_08058

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016391 RA
      MRERRHPAA NVTTPALAT REWSAVLTC WLSRRVLLL 
      LLVAITYPP PSLNHLPVH SGTCNSRDR YGRTMPRCY 
      MVKKQSNKY KDCWDGAAV AAAAPDSPT EACVAPPYY 
      TPLTSSTGY SHEFIPNGA VSSPPPASK TASGDSPIF 
      RTRSAEETE AAHDLLSLS QSLPPLPAP GVVTIHPTV 
      PHEDSPPSP SPCYYRPLS PEPPLPVTV PCYPPPIVY 
      VVQVPAAPT PPTSECSSD ADFSDRTKL RSVQAQQDI 
      LILPLSPEP DSRLQEPPP PPPQQPAAA EQADKHETV 
      IASSHLNIP DRRKRKNKR TNKSNKAAK NNNDRDTLD 
      HNVDGGKEA KAPKTKFYK VVEEESDDS ADRCPTDSP 
      DSTTTTTTK LNRYSCGEC GRQYATSSN LSRHKQTHR 
      SLDSQSAKK CMTCGKAYL AHSCGVCGK QFSRPWLLQ 
      GHLRSHTGE KPYGCAHCG KAFADRSNL RAHMQTHSG 
      DKNFSCPQC HKTFALKSY LNKHQETAC LLWDDKTKK 
      QGSDVSTTK TLKMTTTVH AETQTITVD
    CDS sequence
    • >TC016391 RA
      atgcgtgag cgtcgccac cccgctgcc aacgtcacg 
      acgccagca cttgcaacc agggagtgg tcggcggtg 
      cttacctgt tggctaagc cggcgtgtg ctgctgctg 
      ctacttgtt gctatcacc tatcctccg ccatcactc 
      aaccacctg ccggtgcat tccgggacc tgcaacagc 
      agagatcga tacggccgc acgatgccg cgctgctac 
      atggtgaag aagcagagc aacaagtac aaggactgc 
      tgggacggg gcggcggtg gccgccgcg gcgcccgac 
      agccccacc gaggcctgc gtggcgccc ccttactac 
      acgcccctc accagcagc acaggctac tctcatgag 
      ttcatcccc aacggagcg gtttcatcg ccgccgcca 
      gcctccaaa accgcctcc ggcgactcc cccatcttc 
      cgcacccgc agcgccgaa gagaccgaa gccgctcac 
      gacctcctc tccctctcg caaagcctg cccccgctc 
      cccgccccc ggggtggtc accatccac cccaccgtc 
      ccccacgaa gacagcccc ccgtcgccc agtccttgc 
      tactaccgg ccgctgtcc ccggagccc ccactgccc 
      gtcaccgtg ccctgctac ccgcccccg atcgtgtac 
      gtggtgcag gtgcccgcc gcccccacg ccgcccacc 
      tccgagtgc tcctccgac gccgacttc agcgacagg 
      accaagctg aggagcgtt caagcgcag caggatatt 
      ctgatcctg ccgctgtcg ccggagccc gatagcagg 
      ctgcaggag ccgccgccg ccgccgccc caacagccc 
      gccgccgcc gaacaggcc gacaagcac gaaacagtg 
      atagcatct tcccatctc aacattccg gacagacgg 
      aagcggaaa aataaaagg accaataaa agcaacaag 
      gcggcgaaa aataacaat gatagggac acgctcgat 
      cataatgtt gatggggga aaggaggcc aaagcaccc 
      aaaacgaaa ttctacaaa gtggtcgaa gaggagtcg 
      gacgactcc gcggaccgg tgccccacc gactccccc 
      gactcgacg acgacgacg acgacgaaa ttgaatcga 
      tactcctgc ggcgaatgc ggaagacag tacgccacg 
      tccagcaac ctgtcgcgt cacaaacaa acccatcgc 
      agcttggac tcgcaatcg gcgaagaag tgcatgacg 
      tgcgggaag gcctacctg gcgcacagc tgcggcgtc 
      tgcggaaag cagttctca aggccttgg ctgctccaa 
      ggccactta aggtcgcac actggagag aagccctac 
      ggctgtgcc cactgtggc aaggccttc gctgatcgg 
      tcgaatcta agggcgcac atgcagacg cattcaggc 
      gataaaaat ttcagctgt ccgcagtgc cacaaaacg 
      ttcgcgctc aagtcatac ctgaacaag caccaggaa 
      acggcctgt ttgctgtgg gacgacaag accaagaag 
      caaggaagc gacgtttcg acaacaaaa acgctgaag 
      atgacaacg acggtgcac gccgaaacg cagaccatc 
      accgtcgat taa
    mRNA sequence
    • >TC016391 RA
      ctgctccca tgaaaagag acaggcgag cgtcggtgg 
      ttcggtaac aggtgtcgg tccggatcg atcccacgc 
      gctttctgg cgcatgcgt gagcgtcgc caccccgct 
      gccaacgtc acgacgcca gcacttgca accagggag 
      tggtcggcg gtgcttacc tgttggcta agccggcgt 
      gtgctgctg ctgctactt gttgctatc acctatcct 
      ccgccatca ctcaaccac ctgccggtg cattccggg 
      acctgcaac agcagagat cgatacggc cgcacgatg 
      ccgcgctgc tacatggtg aagaagcag agcaacaag 
      tacaaggac tgctgggac ggggcggcg gtggccgcc 
      gcggcgccc gacagcccc accgaggcc tgcgtggcg 
      cccccttac tacacgccc ctcaccagc agcacaggc 
      tactctcat gagttcatc cccaacgga gcggtttca 
      tcgccgccg ccagcctcc aaaaccgcc tccggcgac 
      tcccccatc ttccgcacc cgcagcgcc gaagagacc 
      gaagccgct cacgacctc ctctccctc tcgcaaagc 
      ctgcccccg ctccccgcc cccggggtg gtcaccatc 
      caccccacc gtcccccac gaagacagc cccccgtcg 
      cccagtcct tgctactac cggccgctg tccccggag 
      cccccactg cccgtcacc gtgccctgc tacccgccc 
      ccgatcgtg tacgtggtg caggtgccc gccgccccc 
      acgccgccc acctccgag tgctcctcc gacgccgac 
      ttcagcgac aggaccaag ctgaggagc gttcaagcg 
      cagcaggat attctgatc ctgccgctg tcgccggag 
      cccgatagc aggctgcag gagccgccg ccgccgccg 
      ccccaacag cccgccgcc gccgaacag gccgacaag 
      cacgaaaca gtgatagca tcttcccat ctcaacatt 
      ccggacaga cggaagcgg aaaaataaa aggaccaat 
      aaaagcaac aaggcggcg aaaaataac aatgatagg 
      gacacgctc gatcataat gttgatggg ggaaaggag 
      gccaaagca cccaaaacg aaattctac aaagtggtc 
      gaagaggag tcggacgac tccgcggac cggtgcccc 
      accgactcc cccgactcg acgacgacg acgacgacg 
      aaattgaat cgatactcc tgcggcgaa tgcggaaga 
      cagtacgcc acgtccagc aacctgtcg cgtcacaaa 
      caaacccat cgcagcttg gactcgcaa tcggcgaag 
      aagtgcatg acgtgcggg aaggcctac ctggcgcac 
      agctgcggc gtctgcgga aagcagttc tcaaggcct 
      tggctgctc caaggccac ttaaggtcg cacactgga 
      gagaagccc tacggctgt gcccactgt ggcaaggcc 
      ttcgctgat cggtcgaat ctaagggcg cacatgcag 
      acgcattca ggcgataaa aatttcagc tgtccgcag 
      tgccacaaa acgttcgcg ctcaagtca tacctgaac 
      aagcaccag gaaacggcc tgtttgctg tgggacgac 
      aagaccaag aagcaagga agcgacgtt tcgacaaca 
      aaaacgctg aagatgaca acgacggtg cacgccgaa 
      acgcagacc atcaccgtc gattaagta tttttgacg 
      atgacatta cacgattac gagaaaaaa aaatgaaac 
      attttcgga gaggtgagc ttgttttga ttggaatac 
      agaatgatc caaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY