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Gene information
General information
TC002437 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29121986..29128288
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002437
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08055

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002437 RA
      MGNTHAKPH PKPRNKKQV HWKAADRPS PPGKPHLSD 
      NDLTPDLVT IVWSKPTRD GGSPITGYL VEHRRTGSP 
      HWVRATPLL VPFPELTLS GLEPGWRYQ FRVRAENAV 
      GLSEPSDIS EPLTVTLQK FAITAPKFT EELKDATAL 
      ENEKVEFVV HFLGQPAPK ICWFKDGFE IFSSRRTRI 
      LTENDRSVL TIHQSALSD EGEIKCTAT NKAGHASTK 
      ARLSLEAPP SIRLPRQYE DGLLFEIGE VIRLKVSVA 
      GRPTPLVFW SHDGESIQN NDRYEIEYV DKCSILKIA 
      EATRGDRGE YQIKAVNKI GEDLASFLV TITDKPSPP 
      GRARVVMTL GRSVTLSWS TPDDDGGCK IGNYIVEYY 
      RLGWNVWLK AATSRQLTT ILGDLIEGS EYKFRIKAE 
      SPYGISEPG AESDIVFIP DPKRGITSP QARSRSQPK 
      DIIDEITSV PVAAKRRPK PRSQSSITK AETNYYDEM 
      PKRPERNKI KSPPKTPEV VRKNASPQT ILDRASLAR 
      ELAYGSPEI KMLQSERPT SPLPPQTKS PSPPKSRTP 
      SPNLTPSPS EDKSKTPSP KDKRSLLRE KSETFEESS 
      EFMLVLYPD ANEKGRRRS SQNIEFDFD EYDIPPPLS 
      LSAPELGAE PPVFENLKT SASSTELLH ERAMMRFNE 
      AAAAEEEEL KKRRKFSYD GQHNIDIPK IQINSKDDQ 
      DIVGLERKP SRRKSSGSV VQQQLLWAQ KRFSLKNSN 
      DLNEFIETK LQKTQIPSP EAPKKEMVR QRSESEERE 
      EEEFNRVRA KMGLQGQSS LEKRSIEVV DEEKWLEDY 
      EESLSESET ESEDERDYD IQPPQRIYS DEDEETYHP 
      GVMTTPRKP SVSDEPFEI LTKRNKLPD PNFVPKPIL 
      KKTEVEPPK VSSTKIRSH SPMPQFRQE VTRGRLQSL 
      AQPPIELSD NKSKVFQRS ISLSTEENT ESEKIAPTL 
      QAAPGRNIS AVATLCGIT AASIVIPEK LLQKKNDEE 
      ESKVVVDHY MDIVRSYGQ RKKSNPEVW KAATNEAKT 
      WSTTKEEKT EEEAQTLMS YGQRKNSNP QIRKVLEET 
      KPEAKTWST NKEEKDEEL TEIVKSSGQ RKNSVPQSL 
      KEEVKTWNT TKEEKSEVQ PLRTERSPS PSPQKTAPN 
      QKRGRSGVN HKPRRGSSR SKTPSRSPS RLENWSGRK 
      SSPSPMKRK TSPSPMRAR KSPSPIPLA KPRPKLREI 
      TTQTSIGLE LNYSSDSRA STPHDRRQE ELLAKAEVK 
      VRSFVDYLT DLAMFFVAC WLYLFSNEL LAIPVLLVM 
      VYRQLKNEI GKRIPRWIV RRFSKKKAK K
    CDS sequence
    • >TC002437 RA
      atggggaac acacacgcg aagccccat ccaaaaccg 
      aggaataaa aaacaagta cactggaag gctgcagac 
      cgcccctcc ccgcccggc aagccccac ctctccgac 
      aacgacctc acccccgac ctcgtcacc atcgtctgg 
      tctaagcca acccgcgat ggcggctct ccaatcacc 
      ggctacctc gtcgagcac cgccgcaca ggctccccc 
      cactgggtg cgagccacg cccctcctc gtccccttc 
      cccgagctc accctcagc ggcctggag cccggctgg 
      cgctaccag ttccgggtg cgggccgaa aacgcagtt 
      ggcctttcc gaacccagc gacatctcc gaaccctta 
      actgtaacg ttgcaaaaa ttcgctatt acagcacca 
      aagttcacc gaagaactc aaagacgca acagcgctc 
      gaaaatgag aaagttgag ttcgttgtc cactttttg 
      ggacaacct gcgccgaaa atctgctgg tttaaggac 
      gggtttgag atttttagc agcagaagg acgaggatt 
      ttgacggag aatgatcgg agtgtgttg actatacac 
      cagagcgcg ctgtcggat gagggcgag atcaagtgc 
      acagcgacc aataaggcg gggcatgcg tccacgaag 
      gcgcggcta agcttggaa gccccgccc agtatacgc 
      ctaccgaga caatacgaa gatgggctg ttgtttgag 
      atcggggaa gttattagg ttgaaggtt tctgtggct 
      ggccgtccg actccgttg gttttttgg agccatgat 
      ggagaatcg attcaaaat aacgaccga tatgagatc 
      gaatacgtg gacaagtgt tccattttg aaaattgct 
      gaagcgaca agaggtgat cgaggggag taccagatc 
      aaagctgtg aacaagatt ggagaggat ttggcctcg 
      tttttggtc accatcacc gacaagcct tcgccacca 
      ggtcgggca agagtggtt atgaccttg ggccgttcc 
      gtcaccctc tcctggagc acccctgac gatgatggg 
      ggttgtaaa atcgggaac tacatcgta gagtactac 
      agactgggg tggaacgtc tggctcaaa gctgctact 
      agtagacaa ctgaccaca atcctaggg gacttaatc 
      gaaggaagc gagtacaaa ttcaggatc aaggccgaa 
      agtccgtac gggattagc gaaccgggg gctgaatcc 
      gacatcgtc ttcatccct gatcccaaa agagggatc 
      acttcgccc caagcgcga agccgaagc caaccgaag 
      gatatcata gacgaaatc acttcagtc ccagttgcg 
      gccaaacga cgtccaaaa cccaggtcg cagtcatca 
      ataaccaaa gcagaaaca aattactat gacgagatg 
      cccaaacgt ccagaacgt aacaaaatc aaaagtcct 
      cccaaaaca ccagaagtt gtacgaaag aacgcaagt 
      cctcaaact atcctcgat cgggcttcg ctagctcgg 
      gaattggcg tacggaagt ccagaaatt aaaatgttg 
      cagtcagaa aggccgacc agtcctttg ccaccacaa 
      accaaaagc ccaagtccg ccaaaatcg cgaactccg 
      agtcctaat ctcaccccg tctccctct gaagataag 
      agcaagacc ccaagtccg aaagacaag aggagtttg 
      cttcgggag aagtccgaa acgttcgaa gagagttcg 
      gagtttatg ctggtctta tatccggat gctaacgaa 
      aaaggtcgt cgtcggtcc agtcaaaac atcgaattc 
      gactttgac gagtacgat atcccccct ccactctcc 
      ctctcagct ccagagctc ggcgcagaa ccgcctgtt 
      ttcgaaaac ttgaaaaca tcagcgagt tccaccgaa 
      ctgttgcac gaacgcgcc atgatgcgc ttcaacgaa 
      gccgcagcc gctgaagaa gaagagttg aagaaacga 
      agaaagttc agttatgac ggccaacac aacatcgac 
      atacccaaa atccaaatc aactcaaag gatgatcag 
      gatatcgtc ggtctggaa cggaaacct tcccgaagg 
      aaatcgtcg gggagtgtg gtccagcag cagctgctc 
      tgggcccag aaacgcttc agtttgaag aattcgaac 
      gatttgaat gaatttatc gaaacgaaa ttgcaaaaa 
      acgcaaatt cctagtccc gaagcgccc aagaaggaa 
      atggtgcgg caaagatcg gagtctgag gaacgcgag 
      gaagaggaa tttaaccga gtgagggca aaaatgggg 
      ctccaaggc cagtctagt ttggaaaaa cgcagcatt 
      gaagtggtg gatgaagaa aagtggttg gaggattac 
      gaagaaagt ttatcagag tcggaaact gaatctgaa 
      gatgagcgg gattacgac atccaaccc cctcaaaga 
      atctacagt gatgaagat gaagaaacg taccaccct 
      ggcgtcatg accacgccc agaaaacca tcagttagt 
      gacgaaccg tttgaaatt ttgaccaaa agaaacaaa 
      ctacctgat ccgaacttc gtaccaaaa ccaattctg 
      aagaaaact gaagtggaa ccaccgaaa gtttcaagt 
      accaaaata aggtcacac tcgcccatg cctcagttt 
      cggcaagaa gtgactaga gggcgtttg caatctttg 
      gcacaacca ccaatagaa ttgtcggat aacaaaagc 
      aaggttttc caacgctcg atttccctc tccactgaa 
      gaaaacaca gaaagtgaa aaaatcgcc ccaacactt 
      caggccgct ccaggacgg aatatttca gcagtggcg 
      actctttgt ggtattacg gctgccagt atcgtcata 
      ccggaaaaa ctgcttcag aagaaaaat gatgaagaa 
      gagtcgaag gttgttgtg gaccactac atggacatt 
      gtgaggagt tatggacaa cgaaagaag agtaatccc 
      gaagtttgg aaagcggca acaaacgaa gcaaagacc 
      tggagtacc accaaagaa gaaaaaact gaagaagaa 
      gcacaaact ttgatgagt tatggccaa cgaaagaat 
      agtaatccc caaattagg aaggtatta gaagaaaca 
      aaaccagaa gctaaaacc tggagtacc aacaaagaa 
      gaaaaagat gaggaacta acagagatt gtcaagagt 
      tctggtcaa aggaagaat agtgttccc caaagtttg 
      aaggaagaa gtgaagact tggaacacc accaaagaa 
      gaaaaaagc gaagttcaa cctctcagg accgaacgt 
      agtccctct ccctcccct caaaaaacc gctccgaac 
      cagaaaaga gggagaagc ggcgtcaac cacaaacct 
      aggagaggt tccagtcgt tccaaaacc ccctctcgt 
      tccccttcc agactggaa aactggtca ggacgcaag 
      tccagccct tcgcccatg aaacgcaaa acttccccc 
      tctcccatg agggccagg aaatcacca tcgcccatc 
      cctttggcc aaacctcgg cccaaacta cgtgaaatc 
      acaactcag acgtccatc ggtttagaa ttgaactat 
      tcgagcgat tctcgagct tccactcct cacgacagg 
      agacaggag gagcttttg gctaaagct gaagtcaaa 
      gttcgcagc tttgtcgat tatcttacg gatctggcg 
      atgtttttc gtggcttgt tggttgtac ttgttcagt 
      aatgaactc ttagcgatt cctgttttg ttggtcatg 
      gtttacaga cagttgaag aatgagatc gggaaaagg 
      ataccacga tggattgtt agacgattt agtaaaaag 
      aaagcgaag aagtaa
    mRNA sequence
    • >TC002437 RA
      caagttatt tttaacata gcacagtgt ggtgacggt 
      tttgccgag atgatagtg tgcgatggg gaacacaca 
      cgcgaagcc ccatccaaa accgaggaa taaaaaaca 
      agtacactg gaaggctgc agaccgccc ctccccgcc 
      cggcaagcc ccacctctc cgacaacga cctcacccc 
      cgacctcgt caccatcgt ctggtctaa gccaacccg 
      cgatggcgg ctctccaat caccggcta cctcgtcga 
      gcaccgccg cacaggctc cccccactg ggtgcgagc 
      cacgcccct cctcgtccc cttccccga gctcaccct 
      cagcggcct ggagcccgg ctggcgcta ccagttccg 
      ggtgcgggc cgaaaacgc agttggcct ttccgaacc 
      cagcgacat ctccgaacc cttaactgt aacgttgca 
      aaaattcgc tattacagc accaaagtt caccgaaga 
      actcaaaga cgcaacagc gctcgaaaa tgagaaagt 
      tgagttcgt tgtccactt tttgggaca acctgcgcc 
      gaaaatctg ctggtttaa ggacgggtt tgagatttt 
      tagcagcag aaggacgag gattttgac ggagaatga 
      tcggagtgt gttgactat acaccagag cgcgctgtc 
      ggatgaggg cgagatcaa gtgcacagc gaccaataa 
      ggcggggca tgcgtccac gaaggcgcg gctaagctt 
      ggaagcccc gcccagtat acgcctacc gagacaata 
      cgaagatgg gctgttgtt tgagatcgg ggaagttat 
      taggttgaa ggtttctgt ggctggccg tccgactcc 
      gttggtttt ttggagcca tgatggaga atcgattca 
      aaataacga ccgatatga gatcgaata cgtggacaa 
      gtgttccat tttgaaaat tgctgaagc gacaagagg 
      tgatcgagg ggagtacca gatcaaagc tgtgaacaa 
      gattggaga ggatttggc ctcgttttt ggtcaccat 
      caccgacaa gccttcgcc accaggtcg ggcaagagt 
      ggttatgac cttgggccg ttccgtcac cctctcctg 
      gagcacccc tgacgatga tgggggttg taaaatcgg 
      gaactacat cgtagagta ctacagact ggggtggaa 
      cgtctggct caaagctgc tactagtag acaactgac 
      cacaatcct aggggactt aatcgaagg aagcgagta 
      caaattcag gatcaaggc cgaaagtcc gtacgggat 
      tagcgaacc gggggctga atccgacat cgtcttcat 
      ccctgatcc caaaagagg gatcacttc gccccaagc 
      gcgaagccg aagccaacc gaaggatat catagacga 
      aatcacttc agtcccagt tgcggccaa acgacgtcc 
      aaaacccag gtcgcagtc atcaataac caaagcaga 
      aacaaatta ctatgacga gatgcccaa acgtccaga 
      acgtaacaa aatcaaaag tcctcccaa aacaccaga 
      agttgtacg aaagaacgc aagtcctca aactatcct 
      cgatcgggc ttcgctagc tcgggaatt ggcgtacgg 
      aagtccaga aattaaaat gttgcagtc agaaaggcc 
      gaccagtcc tttgccacc acaaaccaa aagcccaag 
      tccgccaaa atcgcgaac tccgagtcc taatctcac 
      cccgtctcc ctctgaaga taagagcaa gaccccaag 
      tccgaaaga caagaggag tttgcttcg ggagaagtc 
      cgaaacgtt cgaagagag ttcggagtt tatgctggt 
      cttatatcc ggatgctaa cgaaaaagg tcgtcgtcg 
      gtccagtca aaacatcga attcgactt tgacgagta 
      cgatatccc ccctccact ctccctctc agctccaga 
      gctcggcgc agaaccgcc tgttttcga aaacttgaa 
      aacatcagc gagttccac cgaactgtt gcacgaacg 
      cgccatgat gcgcttcaa cgaagccgc agccgctga 
      agaagaaga gttgaagaa acgaagaaa gttcagtta 
      tgacggcca acacaacat cgacatacc caaaatcca 
      aatcaactc aaaggatga tcaggatat cgtcggtct 
      ggaacggaa accttcccg aaggaaatc gtcggggag 
      tgtggtcca gcagcagct gctctgggc ccagaaacg 
      cttcagttt gaagaattc gaacgattt gaatgaatt 
      tatcgaaac gaaattgca aaaaacgca aattcctag 
      tcccgaagc gcccaagaa ggaaatggt gcggcaaag 
      atcggagtc tgaggaacg cgaggaaga ggaatttaa 
      ccgagtgag ggcaaaaat ggggctcca aggccagtc 
      tagtttgga aaaacgcag cattgaagt ggtggatga 
      agaaaagtg gttggagga ttacgaaga aagtttatc 
      agagtcgga aactgaatc tgaagatga gcgggatta 
      cgacatcca accccctca aagaatcta cagtgatga 
      agatgaaga aacgtacca ccctggcgt catgaccac 
      gcccagaaa accatcagt tagtgacga accgtttga 
      aattttgac caaaagaaa caaactacc tgatccgaa 
      cttcgtacc aaaaccaat tctgaagaa aactgaagt 
      ggaaccacc gaaagtttc aagtaccaa aataaggtc 
      acactcgcc catgcctca gtttcggca agaagtgac 
      tagagggcg tttgcaatc tttggcaca accaccaat 
      agaattgtc ggataacaa aagcaaggt tttccaacg 
      ctcgatttc cctctccac tgaagaaaa cacagaaag 
      tgaaaaaat cgccccaac acttcaggc cgctccagg 
      acggaatat ttcagcagt ggcgactct ttgtggtat 
      tacggctgc cagtatcgt cataccgga aaaactgct 
      tcagaagaa aaatgatga agaagagtc gaaggttgt 
      tgtggacca ctacatgga cattgtgag gagttatgg 
      acaacgaaa gaagagtaa tcccgaagt ttggaaagc 
      ggcaacaaa cgaagcaaa gacctggag taccaccaa 
      agaagaaaa aactgaaga agaagcaca aactttgat 
      gagttatgg ccaacgaaa gaatagtaa tccccaaat 
      taggaaggt attagaaga aacaaaacc agaagctaa 
      aacctggag taccaacaa agaagaaaa agatgagga 
      actaacaga gattgtcaa gagttctgg tcaaaggaa 
      gaatagtgt tccccaaag tttgaagga agaagtgaa 
      gacttggaa caccaccaa agaagaaaa aagcgaagt 
      tcaacctct caggaccga acgtagtcc ctctccctc 
      ccctcaaaa aaccgctcc gaaccagaa aagagggag 
      aagcggcgt caaccacaa acctaggag aggttccag 
      tcgttccaa aaccccctc tcgttcccc ttccagact 
      ggaaaactg gtcaggacg caagtccag cccttcgcc 
      catgaaacg caaaacttc cccctctcc catgagggc 
      caggaaatc accatcgcc catcccttt ggccaaacc 
      tcggcccaa actacgtga aatcacaac tcagacgtc 
      catcggttt agaattgaa ctattcgag cgattctcg 
      agcttccac tcctcacga caggagaca ggaggagct 
      tttggctaa agctgaagt caaagttcg cagctttgt 
      cgattatct tacggatct ggcgatgtt tttcgtggc 
      ttgttggtt gtacttgtt cagtaatga actcttagc 
      gattcctgt tttgttggt catggttta cagacagtt 
      gaagaatga gatcgggaa aaggatacc acgatggat 
      tgttagacg atttagtaa aaagaaagc gaagaagta 
      ataacatgt actgtttgc acggttttt ttctattgt 
      ttgtgtgag attttccga acacgacgt tatgaaatc 
      tatgatttc acttatctt atttaggca gtgattgtg 
      tgtactttc aattaagtg gtgtacata atttttatt 
      aagcgaaac gttatggtt tcaccggcc tttaaataa 
      ataacataa atccca
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