Status
Please wait ...
Gene information
General information
TC032646
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002473,TC002475
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 27699219..27759382
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032646
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08051

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: In most of the cases,  hind gut of larvae are outside.
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032646 RA
      MSPYSRYRP LPGSDGISV LQTPPGCRP TPPTACRFL 
      RWISKFSPQ PVNKQTLGV LDMTATENT IRFSVHTLD 
      KATKAKVTL ENYYTNLIA QHVERKQRL AKLEESLKD 
      DSLSEEQKH EKRLQHAQK ETEFLRLKR SRLGVEDFE 
      PLKVIGRGA FGEVRLVQK KDTGHVYAM KILRKADML 
      EKEQVAHVR AERDILVEA DHQWVVKMY YSFQDPINL 
      YLIMEFLPG GDMMTLLMK KDTLSEEFT QFYIAETAL 
      AIDSIHKLG FIHRDIKPD NLLLDAKGH LKLSDFGLC 
      TGLKKSHRT DFYRDLSQA KPSDFIITS SPMDSKRRA 
      ESWKKNRRA LAYSTVGTP DYIAPEVFL QTGYGPACD 
      WWSLGVIMY EMLIGYPPF CSENPQDTY RKVMNWRET 
      LVFPAEVPI SEEAKDTII KFCCEAERR LGSQKGIED 
      LKIIPFFRG VDWEHIRER PAAIPVEVK SIDDTSNFD 
      DFPDVKLEI PSAPMPQDG EVNYKDWVF INYTFKRFE 
      GLTQRGTPT KK
    CDS sequence
    • >TC032646 RA
      atgtcacca tacagtcgg taccggcct ttgccgggc 
      tctgacggg atttcggtg cttcagaca ccaccagga 
      tgtaggccg acgccaccg acggcctgt cgcttcctc 
      aggtggata tctaagttc agtccgcaa ccggtgaac 
      aaacagact ttaggggtc ctggacatg actgccacg 
      gagaataca atccgtttc agtgtccac accctggac 
      aaagccacc aaagctaaa gtcacccta gaaaattac 
      tacacaaat ctcatagcc cagcatgtg gagcgaaaa 
      caaaggcta gcgaagctc gaagaatcc ttgaaagac 
      gacagcctc tccgaagag cagaagcat gagaagcgc 
      ctccagcac gcccaaaaa gaaacggaa ttcctccga 
      ctgaagcgc tctcgtctc ggtgtcgaa gacttcgaa 
      ccgttgaaa gtaatcggt cggggagct ttcggcgaa 
      gtccgcctg gtccagaaa aaggacacc ggtcacgtc 
      tacgccatg aagatcctc cgtaaagcc gacatgctc 
      gaaaaggag caagtggcg cacgtgcga gccgaacgc 
      gacatcctt gtcgaagcc gaccaccag tgggtggtc 
      aaaatgtat tatagtttt caagatccg atcaatttg 
      tatttgatt atggagttt ttgcctggc ggtgatatg 
      atgacgctg ttgatgaaa aaagacacg ttgtcggag 
      gaatttacg cagttctat atcgccgaa acggcacta 
      gctatagat tcgatacat aagctcggg tttatccat 
      cgagatata aagccggat aacttgctg ttggatgcg 
      aaagggcat ttgaaattg tccgatttc ggactgtgt 
      acgggactg aaaaagtcg cacaggacg gatttttat 
      cgggatctg agtcaggcc aagccgtcg gattttata 
      ataacctct agtccgatg gatagtaaa cgtcgtgcc 
      gaaagttgg aagaaaaac cgacgagcg ctggcttac 
      agcaccgtc ggaacgcct gattatatc gcccccgaa 
      gtgttcctt caaaccggc tacggtccg gcgtgtgat 
      tggtggtcg ttaggcgtc atcatgtac gaaatgttg 
      atcggttat ccgccgttc tgttccgaa aatcctcaa 
      gacacttat cgcaaagtg atgaactgg agagagacg 
      cttgtcttt cccgccgaa gttccgatc tctgaagag 
      gccaaagat actattatt aagttttgc tgtgaagcc 
      gaacgaagg ttgggctca cagaagggt atagaagat 
      ttgaaaata attcccttc tttcgggga gttgactgg 
      gaacatatt agggaaagg ccagccgcg atacctgta 
      gaagttaag tcgatagat gatacgtcg aatttcgac 
      gatttccca gacgtcaag cttgaaatt ccttctgcg 
      cctatgcct caggacggt gaggtcaac tacaaagac 
      tgggtgttc atcaactac acgttcaag cggttcgaa 
      ggtctgacg caaagaggg accccaaca aaaaaataa
    mRNA sequence
    • >TC032646 RA
      cgatttgct tcgcgtttt gctctgtcg tcgctcgtg 
      caacaggtg aacatgtgc ttgaatgat cgcgcgaga 
      taaataaat caatccacg aattgattc ttctttccc 
      caacttgga gttacagtg ttacggtgg ttgtttgtt 
      tgattgagg tggtgatgt gaagttttt tgatgtcac 
      catacagtc ggtaccggc ctttgccgg gctctgacg 
      ggatttcgg tgcttcaga caccaccag gatgtaggc 
      cgacgccac cgacggcct gtcgcttcc tcaggtgga 
      tatctaagt tcagtccgc aaccggtga acaaacaga 
      ctttagggg tcctggaca tgactgcca cggagaata 
      caatccgtt tcagtgtcc acaccctgg acaaagcca 
      ccaaagcta aagtcaccc tagaaaatt actacacaa 
      atctcatag cccagcatg tggagcgaa aacaaaggc 
      tagcgaagc tcgaagaat ccttgaaag acgacagcc 
      tctccgaag agcagaagc atgagaagc gcctccagc 
      acgcccaaa aagaaacgg aattcctcc gactgaagc 
      gctctcgtc tcggtgtcg aagacttcg aaccgttga 
      aagtaatcg gtcggggag ctttcggcg aagtccgcc 
      tggtccaga aaaaggaca ccggtcacg tctacgcca 
      tgaagatcc tccgtaaag ccgacatgc tcgaaaagg 
      agcaagtgg cgcacgtgc gagccgaac gcgacatcc 
      ttgtcgaag ccgaccacc agtgggtgg tcaaaatgt 
      attatagtt ttcaagatc cgatcaatt tgtatttga 
      ttatggagt ttttgcctg gcggtgata tgatgacgc 
      tgttgatga aaaaagaca cgttgtcgg aggaattta 
      cgcagttct atatcgccg aaacggcac tagctatag 
      attcgatac ataagctcg ggtttatcc atcgagata 
      taaagccgg ataacttgc tgttggatg cgaaagggc 
      atttgaaat tgtccgatt tcggactgt gtacgggac 
      tgaaaaagt cgcacagga cggattttt atcgggatc 
      tgagtcagg ccaagccgt cggatttta taataacct 
      ctagtccga tggatagta aacgtcgtg ccgaaagtt 
      ggaagaaaa accgacgag cgctggctt acagcaccg 
      tcggaacgc ctgattata tcgcccccg aagtgttcc 
      ttcaaaccg gctacggtc cggcgtgtg attggtggt 
      cgttaggcg tcatcatgt acgaaatgt tgatcggtt 
      atccgccgt tctgttccg aaaatcctc aagacactt 
      atcgcaaag tgatgaact ggagagaga cgcttgtct 
      ttcccgccg aagttccga tctctgaag aggccaaag 
      atactatta ttaagtttt gctgtgaag ccgaacgaa 
      ggttgggct cacagaagg gtatagaag atttgaaaa 
      taattccct tctttcggg gagttgact gggaacata 
      ttagggaaa ggccagccg cgatacctg tagaagtta 
      agtcgatag atgatacgt cgaatttcg acgatttcc 
      cagacgtca agcttgaaa ttccttctg cgcctatgc 
      ctcaggacg gtgaggtca actacaaag actgggtgt 
      tcatcaact acacgttca agcggttcg aaggtctga 
      cgcaaagag ggaccccaa caaaaaaat aaaccgcac 
      ttttatgtg cgccaacaa atcggcagt taatcgcgc 
      tataacgag cacttattt ttaggaaat atcgtgaaa 
      aaccacacc agagtgctt cgattatga ttttctcgt 
      cttttcaat aactgtacc tatcccgta gttattttc 
      taaaaataa gtaactttg tgtattata ggtctgaag 
      cagttttca cactgtttg ctttgagct gtgagaagc 
      tacgtatct tttgttgta tttacactg atatctcgt 
      taacttgtg ttattgtaa atattacga aaatgacga 
      attgttagt acaaaattg ttgcgaaac tactgccaa 
      ttatttatg tgatgatgt gttgcttcg agggcgggc 
      ggtccgagg gatattttt gtacctaat gattttttc 
      cctatcatt ttaaataca acagaagat ttttgtgta 
      tatgttatt ttttgttta ttgttacaa agtgatacg 
      tttattatt tacttgttt ttttattta tagacaatg 
      tatttcttg atcgcgcgt caagaacta ttttgtata 
      actcgtgaa ggacatgaa ctaattaca ctttttaca 
      ccttcatga ttctgtcag tgagatcat atttttgca 
      attttttat ttcaagacg aacgagcgt agtctagta 
      ggcgggttt agtaatttt cgatcagag tgacctgcg 
      acgtttgac aggcagtac cgccaactg tgtttcaag 
      gttcatggc cactctgat cattttatt agttgtttt 
      gttccaaag aagcacata tgaacaatt gtttttcac 
      taaccaatt agattttgt ggaagtgta tgtgtagtt 
      ttgcaaaca tatcgagca gttttttgg ctgttaaac 
      agtctggtg tgtatgtga ggcagttgg tagacttgg 
      aaattttat agaaagtat taatgttaa gcttgtagc 
      tctctatgc acctttttt tttcaaatt cgtttaact 
      ggtgcaaat tagctagat tatttgtct gatttgtta 
      tttattaca ttggtctcg ctaacagga cttgtacat 
      aatatattt acttccaaa aatgtgtat aacaaagtt 
      tattaaact atgtgcttt aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY