General information | |||
TC032646
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 27699219..27759382 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC032646 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08051 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Comment: In most of the cases, hind gut of larvae are outside. |
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032646 RA
MSPYSRYRP LPGSDGISV LQTPPGCRP TPPTACRFL RWISKFSPQ PVNKQTLGV LDMTATENT IRFSVHTLD KATKAKVTL ENYYTNLIA QHVERKQRL AKLEESLKD DSLSEEQKH EKRLQHAQK ETEFLRLKR SRLGVEDFE PLKVIGRGA FGEVRLVQK KDTGHVYAM KILRKADML EKEQVAHVR AERDILVEA DHQWVVKMY YSFQDPINL YLIMEFLPG GDMMTLLMK KDTLSEEFT QFYIAETAL AIDSIHKLG FIHRDIKPD NLLLDAKGH LKLSDFGLC TGLKKSHRT DFYRDLSQA KPSDFIITS SPMDSKRRA ESWKKNRRA LAYSTVGTP DYIAPEVFL QTGYGPACD WWSLGVIMY EMLIGYPPF CSENPQDTY RKVMNWRET LVFPAEVPI SEEAKDTII KFCCEAERR LGSQKGIED LKIIPFFRG VDWEHIRER PAAIPVEVK SIDDTSNFD DFPDVKLEI PSAPMPQDG EVNYKDWVF INYTFKRFE GLTQRGTPT KK
- >TC032646 RA
atgtcacca tacagtcgg taccggcct ttgccgggc tctgacggg atttcggtg cttcagaca ccaccagga tgtaggccg acgccaccg acggcctgt cgcttcctc aggtggata tctaagttc agtccgcaa ccggtgaac aaacagact ttaggggtc ctggacatg actgccacg gagaataca atccgtttc agtgtccac accctggac aaagccacc aaagctaaa gtcacccta gaaaattac tacacaaat ctcatagcc cagcatgtg gagcgaaaa caaaggcta gcgaagctc gaagaatcc ttgaaagac gacagcctc tccgaagag cagaagcat gagaagcgc ctccagcac gcccaaaaa gaaacggaa ttcctccga ctgaagcgc tctcgtctc ggtgtcgaa gacttcgaa ccgttgaaa gtaatcggt cggggagct ttcggcgaa gtccgcctg gtccagaaa aaggacacc ggtcacgtc tacgccatg aagatcctc cgtaaagcc gacatgctc gaaaaggag caagtggcg cacgtgcga gccgaacgc gacatcctt gtcgaagcc gaccaccag tgggtggtc aaaatgtat tatagtttt caagatccg atcaatttg tatttgatt atggagttt ttgcctggc ggtgatatg atgacgctg ttgatgaaa aaagacacg ttgtcggag gaatttacg cagttctat atcgccgaa acggcacta gctatagat tcgatacat aagctcggg tttatccat cgagatata aagccggat aacttgctg ttggatgcg aaagggcat ttgaaattg tccgatttc ggactgtgt acgggactg aaaaagtcg cacaggacg gatttttat cgggatctg agtcaggcc aagccgtcg gattttata ataacctct agtccgatg gatagtaaa cgtcgtgcc gaaagttgg aagaaaaac cgacgagcg ctggcttac agcaccgtc ggaacgcct gattatatc gcccccgaa gtgttcctt caaaccggc tacggtccg gcgtgtgat tggtggtcg ttaggcgtc atcatgtac gaaatgttg atcggttat ccgccgttc tgttccgaa aatcctcaa gacacttat cgcaaagtg atgaactgg agagagacg cttgtcttt cccgccgaa gttccgatc tctgaagag gccaaagat actattatt aagttttgc tgtgaagcc gaacgaagg ttgggctca cagaagggt atagaagat ttgaaaata attcccttc tttcgggga gttgactgg gaacatatt agggaaagg ccagccgcg atacctgta gaagttaag tcgatagat gatacgtcg aatttcgac gatttccca gacgtcaag cttgaaatt ccttctgcg cctatgcct caggacggt gaggtcaac tacaaagac tgggtgttc atcaactac acgttcaag cggttcgaa ggtctgacg caaagaggg accccaaca aaaaaataa
- >TC032646 RA
cgatttgct tcgcgtttt gctctgtcg tcgctcgtg caacaggtg aacatgtgc ttgaatgat cgcgcgaga taaataaat caatccacg aattgattc ttctttccc caacttgga gttacagtg ttacggtgg ttgtttgtt tgattgagg tggtgatgt gaagttttt tgatgtcac catacagtc ggtaccggc ctttgccgg gctctgacg ggatttcgg tgcttcaga caccaccag gatgtaggc cgacgccac cgacggcct gtcgcttcc tcaggtgga tatctaagt tcagtccgc aaccggtga acaaacaga ctttagggg tcctggaca tgactgcca cggagaata caatccgtt tcagtgtcc acaccctgg acaaagcca ccaaagcta aagtcaccc tagaaaatt actacacaa atctcatag cccagcatg tggagcgaa aacaaaggc tagcgaagc tcgaagaat ccttgaaag acgacagcc tctccgaag agcagaagc atgagaagc gcctccagc acgcccaaa aagaaacgg aattcctcc gactgaagc gctctcgtc tcggtgtcg aagacttcg aaccgttga aagtaatcg gtcggggag ctttcggcg aagtccgcc tggtccaga aaaaggaca ccggtcacg tctacgcca tgaagatcc tccgtaaag ccgacatgc tcgaaaagg agcaagtgg cgcacgtgc gagccgaac gcgacatcc ttgtcgaag ccgaccacc agtgggtgg tcaaaatgt attatagtt ttcaagatc cgatcaatt tgtatttga ttatggagt ttttgcctg gcggtgata tgatgacgc tgttgatga aaaaagaca cgttgtcgg aggaattta cgcagttct atatcgccg aaacggcac tagctatag attcgatac ataagctcg ggtttatcc atcgagata taaagccgg ataacttgc tgttggatg cgaaagggc atttgaaat tgtccgatt tcggactgt gtacgggac tgaaaaagt cgcacagga cggattttt atcgggatc tgagtcagg ccaagccgt cggatttta taataacct ctagtccga tggatagta aacgtcgtg ccgaaagtt ggaagaaaa accgacgag cgctggctt acagcaccg tcggaacgc ctgattata tcgcccccg aagtgttcc ttcaaaccg gctacggtc cggcgtgtg attggtggt cgttaggcg tcatcatgt acgaaatgt tgatcggtt atccgccgt tctgttccg aaaatcctc aagacactt atcgcaaag tgatgaact ggagagaga cgcttgtct ttcccgccg aagttccga tctctgaag aggccaaag atactatta ttaagtttt gctgtgaag ccgaacgaa ggttgggct cacagaagg gtatagaag atttgaaaa taattccct tctttcggg gagttgact gggaacata ttagggaaa ggccagccg cgatacctg tagaagtta agtcgatag atgatacgt cgaatttcg acgatttcc cagacgtca agcttgaaa ttccttctg cgcctatgc ctcaggacg gtgaggtca actacaaag actgggtgt tcatcaact acacgttca agcggttcg aaggtctga cgcaaagag ggaccccaa caaaaaaat aaaccgcac ttttatgtg cgccaacaa atcggcagt taatcgcgc tataacgag cacttattt ttaggaaat atcgtgaaa aaccacacc agagtgctt cgattatga ttttctcgt cttttcaat aactgtacc tatcccgta gttattttc taaaaataa gtaactttg tgtattata ggtctgaag cagttttca cactgtttg ctttgagct gtgagaagc tacgtatct tttgttgta tttacactg atatctcgt taacttgtg ttattgtaa atattacga aaatgacga attgttagt acaaaattg ttgcgaaac tactgccaa ttatttatg tgatgatgt gttgcttcg agggcgggc ggtccgagg gatattttt gtacctaat gattttttc cctatcatt ttaaataca acagaagat ttttgtgta tatgttatt ttttgttta ttgttacaa agtgatacg tttattatt tacttgttt ttttattta tagacaatg tatttcttg atcgcgcgt caagaacta ttttgtata actcgtgaa ggacatgaa ctaattaca ctttttaca ccttcatga ttctgtcag tgagatcat atttttgca attttttat ttcaagacg aacgagcgt agtctagta ggcgggttt agtaatttt cgatcagag tgacctgcg acgtttgac aggcagtac cgccaactg tgtttcaag gttcatggc cactctgat cattttatt agttgtttt gttccaaag aagcacata tgaacaatt gtttttcac taaccaatt agattttgt ggaagtgta tgtgtagtt ttgcaaaca tatcgagca gttttttgg ctgttaaac agtctggtg tgtatgtga ggcagttgg tagacttgg aaattttat agaaagtat taatgttaa gcttgtagc tctctatgc acctttttt tttcaaatt cgtttaact ggtgcaaat tagctagat tatttgtct gatttgtta tttattaca ttggtctcg ctaacagga cttgtacat aatatattt acttccaaa aatgtgtat aacaaagtt tattaaact atgtgcttt aac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
