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Gene information
General information
TC002333 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451823.1: 3008..5328
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002333
    iBeetle screen iB_08036

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002333 RA
      MSLTGRNKT SILDKPLSR GKGEVSLSC 
      FALLFSEVV QYCQNKSHT VPELQNRLH 
      ELGRRVGVK LIDLYFLRE RNGKREIKL 
      LNILLFVKS TLWKALFGR EADKLEHSN 
      DDENTYYLM EKEPLVNRF ISVPRDKSS 
      LNCAVFIAG IIEAVLTGT GFTAKVTAH 
      WHKGTTYMV KFDDTVVSR DKQLEER
    CDS sequence
    • >TC002333 RA
      atgagttta acggggcgc aataagacc agtattttg 
      gataagccc ctgagtcgg ggcaagggc gaggtgtct 
      ttaagctgc tttgctttg ctcttttcg gaagttgtg 
      caatattgt caaaataaa tctcacacg gttccggag 
      ctccagaac agattgcac gagttgggg cgcagagtc 
      ggcgtgaaa ctgatcgat ctttacttc cttagagag 
      agaaatggc aaacgggag atcaagtta ttaaatatc 
      ctcctgttt gttaaatca actttgtgg aaggcgttg 
      tttgggcgc gaagctgat aaattagag cattccaat 
      gatgatgaa aacacctat tatttgatg gaaaaagag 
      cccctagtc aatagattt atttcggtc ccgagggac 
      aaaagtagc ctgaattgt gctgtcttc atcgccggg 
      ataatcgaa gccgtactt acgggaaca ggctttacg 
      gctaaagtg acagcgcat tggcacaaa ggaacgaca 
      tacatggtg aaattcgat gatactgtc gtaagcagg 
      gacaagcaa ctcgaggag agataa
    mRNA sequence
    • >TC002333 RA
      tgtcaccca atctaacca cactttttg tcataaaaa 
      atcaatatg agtttaacg gggcgcaat aagaccagt 
      attttggat aagcccctg agtcggggc aagggcgag 
      gtgtcttta agctgcttt gctttgctc ttttcggaa 
      gttgtgcaa tattgtcaa aataaatct cacacggtt 
      ccggagctc cagaacaga ttgcacgag ttggggcgc 
      agagtcggc gtgaaactg atcgatctt tacttcctt 
      agagagaga aatggcaaa cgggagatc aagttatta 
      aatatcctc ctgtttgtt aaatcaact ttgtggaag 
      gcgttgttt gggcgcgaa gctgataaa ttagagcat 
      tccaatgat gatgaaaac acctattat ttgatggaa 
      aaagagccc ctagtcaat agatttatt tcggtcccg 
      agggacaaa agtagcctg aattgtgct gtcttcatc 
      gccgggata atcgaagcc gtacttacg ggaacaggc 
      tttacggct aaagtgaca gcgcattgg cacaaagga 
      acgacatac atggtgaaa ttcgatgat actgtcgta 
      agcagggac aagcaactc gaggagaga taatttgtt 
      gattttgta tatttttca ataaattcc tttttcctc 
      aattgtgtt tttctaacc caattccac gaattaatt 
      tctagataa atatttcaa ttatcaaaa gtttccact 
      tgaaaaaat tcgcaaaaa acacaaaac tcattttaa 
      tcgcgctga taattgtgt caaaacatc cggtactta 
      ccctcaagc atgagtaca aaaggacag ttggttggg 
      aaaagacca aaccaaact tttgcccat tttttaatg 
      ccaacaaaa tttacaaat gttactaat ttttttaat 
      aacttcaat taaatcaac ttgaagtcg cgatctttg 
      taagcgact tcgccattt cggtatctc ttctccttg 
      gcctgtctt gtagtgtgg aggaggtag atgtaacca 
      accattggg agcagtgtt aggaagaaa ccgtagcct 
      gcagtttta ggattgggc ttgaaatgc tcgtaagtg 
      ctcactcgc atcgcctgg tagcgctgg agggccggg 
      tcgaactga aaataacaa aaaaaaaaa tattaaaaa 
      aaataaata ttttgagta atg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY