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Gene information
General information
TC034628 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25218025..25228900
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034628
    iBeetle screen iB_08034

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034628 RA
      MANSNRGKP IVVENIATF RRSQSPLDV FVIPELSQK 
      RKLTDVPFL ITLAICVVV LMIAMIYTL YYSDMNRFT 
      NGYDNCGNV CGEKNSHVD GIPCSGQDM TNKPYLVGG 
      KCVERCADA DCSTSDVWN EFNEKLGPM AIVCCIGLG 
      LSIIALFCF RYFVDVFVW TMLIGAVLL MCALTGYIW 
      YCYANQSSE QGSGLLIGA IIFTFISLI FAVFILVLK 
      DMIKLIIRF YEETIKMVF AMPLILLEP VLTFISIFV 
      IVGLCVYTF ILMNHAGKL QETGDSSYP YRIKASRYT 
      KDSVLPVVI GAVFAFLCI HCIFVVFEI DVDAVFICY 
      CEDNCLNNG TTEPFYATV EFYQQVKKA QAVTKSIEA 
      KKKKK
    CDS sequence
    • >TC034628 RA
      atggcaaat tcaaatcgg ggaaaaccg attgttgtt 
      gaaaatatc gcaaccttt aggagaagt cagtcacct 
      ttggacgtt tttgtcatt cctgaactc tcccaaaaa 
      agaaaatta actgacgtg cccttcctt ataacatta 
      gcaatatgt gttgtggtt ttgatgata gcaatgata 
      tacacttta tattattcg gatatgaac agatttacg 
      aatgggtat gacaactgt ggtaatgtt tgtggtgaa 
      aaaaatagt catgttgat gggataccc tgttctggt 
      caagacatg acgaacaag ccatatcta gttggcggg 
      aagtgtgtt gagagatgc gctgatgca gactgtagc 
      acctcggat gtctggaac gaattcaac gaaaagttg 
      ggacctatg gcaatagtt tgttgcatt ggacttggt 
      ctttccatt atcgcatta ttttgcttt cgctatttt 
      gtggacgta tttgtttgg actatgctg ataggtgct 
      gtcttacta atgtgcgca ctcacagga tatatttgg 
      tattgctac gccaatcag tcatcagaa caaggaagc 
      ggcttattg ataggggct atcattttc acattcatt 
      tcgcttatt tttgcagta tttattcta gtgctgaaa 
      gacatgatt aaactaata atcaggttt tacgaggaa 
      accatcaaa atggtattc gctatgcct ctaattctt 
      ctagaacct gtcttgact tttatctcc atttttgtg 
      attgttgga ttatgcgtt tacaccttt attttaatg 
      aaccacgct ggaaaattg caagaaact ggtgactca 
      tcctatccg tatcgtatc aaagccagt agatatact 
      aaagattcc gtcctacct gttgtaatt ggagctgtg 
      tttgcgttt ttgtgcatt cactgtatc ttcgttgtt 
      ttcgagatt gatgtagat gcagtattc atctgttat 
      tgtgaagat aactgcctt aataatggt acaactgaa 
      ccattttat gcaactgtg gagttttac cagcaggtg 
      aagaaggcg caagccgtg acgaaaagt attgaagca 
      aagaagaaa aagaaatga
    mRNA sequence
    • >TC034628 RA
      ttacacttt tgcgtttga ttgatttag aggacgagg 
      agattgtag tagaagttt aaaattgta tttgtgtag 
      tgatcataa ttctgataa tggcaaatt caaatcggg 
      gaaaaccga ttgttgttg aaaatatcg caaccttta 
      ggagaagtc agtcacctt tggacgttt ttgtcattc 
      ctgaactct cccaaaaaa gaaaattaa ctgacgtgc 
      ccttcctta taacattag caatatgtg ttgtggttt 
      tgatgatag caatgatat acactttat attattcgg 
      atatgaaca gatttacga atgggtatg acaactgtg 
      gtaatgttt gtggtgaaa aaaatagtc atgttgatg 
      ggataccct gttctggtc aagacatga cgaacaagc 
      catatctag ttggcggga agtgtgttg agagatgcg 
      ctgatgcag actgtagca cctcggatg tctggaacg 
      aattcaacg aaaagttgg gacctatgg caatagttt 
      gttgcattg gacttggtc tttccatta tcgcattat 
      tttgctttc gctattttg tggacgtat ttgtttgga 
      ctatgctga taggtgctg tcttactaa tgtgcgcac 
      tcacaggat atatttggt attgctacg ccaatcagt 
      catcagaac aaggaagcg gcttattga taggggcta 
      tcattttca cattcattt cgcttattt ttgcagtat 
      ttattctag tgctgaaag acatgatta aactaataa 
      tcaggtttt acgaggaaa ccatcaaaa tggtattcg 
      ctatgcctc taattcttc tagaacctg tcttgactt 
      ttatctcca tttttgtga ttgttggat tatgcgttt 
      acaccttta ttttaatga accacgctg gaaaattgc 
      aagaaactg gtgactcat cctatccgt atcgtatca 
      aagccagta gatatacta aagattccg tcctacctg 
      ttgtaattg gagctgtgt ttgcgtttt tgtgcattc 
      actgtatct tcgttgttt tcgagattg atgtagatg 
      cagtattca tctgttatt gtgaagata actgcctta 
      ataatggta caactgaac cattttatg caactgtgg 
      agttttacc agcaggtga agaaggcgc aagccgtga 
      cgaaaagta ttgaagcaa agaagaaaa agaaatgaa 
      ataacttat caagtgttt gaattaaat aaacgtaaa 
      ctgtttgtt aaatattta ttcattagg tgtaactac 
      accctgtaa actactatg tccttataa ttcatgtaa 
      aaatgaaaa atacgctta atacatacg attattatt 
      tttttttga agagtacct acctaaata aaattagtc 
      taaatgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY