Status
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Gene information
General information
TC032588
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010347
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24242688..24247461
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032588
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08028

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032588 RA
      MKLCFSTVI IQNAGVYEI NSDDIAMIF YLTVENTFE 
      CKVHVSVSS LKCIEGDPY SDTADHMFV IGGDMGVIN 
      SKGVKNATL IYPNLYLRN RAGKCLMSL NYQTEQSSE 
      QMIREIYFN TNEIYHKGG GTALDKCST CDLTSETCL 
      PVDCVIKYS SRRSYFNRE RKICQSVPE CPPNTIYVP 
      KSNICRKLH EPVCESDLE MIEDGNIEH CHSISNIVN 
      SKCYHGVLD NGTGECECD DGWATATCA EGAYHPTLN 
      LYHMCNVGF NSWQRANRE KLIMTILVL VILAIALAA 
      KMLLAVCLL NWCCHFIRS RSKSYIDDE FCGSEHCHY 
      SSYCGDADC LQEVPKDKS SRTIRVSFS NISSAEDTD 
      ATASGDDDK THSKSSGNA SHATINLCH CQR
    CDS sequence
    • >TC032588 RA
      atgaagtta tgttttagt acagttatt atacagaac 
      gccggtgtg tacgaaatt aacagtgac gatattgca 
      atgattttt tatttgacc gtagaaaat acgtttgaa 
      tgcaaagtt cacgtaagt gtttccagc ctaaaatgc 
      attgaagga gatccttat tccgacaca gcggaccat 
      atgttcgta attggggga gatatgggg gtcattaat 
      agcaaaggt gtgaaaaac gctacacta atttaccca 
      aatttgtat ttgcgaaat agagctggg aaatgttta 
      atgtcgcta aattaccaa actgaacaa tcatccgag 
      caaatgatc agagaaata tattttaac acgaacgag 
      atttaccat aaaggagga ggtacagcc cttgataaa 
      tgctcgact tgtgatctc acgtctgaa acttgtctt 
      cctgtcgac tgtgttatt aaatactcc tctcgaagg 
      agttacttc aaccgtgag cgtaaaatt tgtcagtcg 
      gttcctgaa tgtccccca aatactatc tacgtcccc 
      aaaagtaac atttgcagg aagttacac gaaccagtg 
      tgtgagtct gatcttgaa atgattgag gatggtaac 
      atcgaacat tgccacagt atttcaaat attgtgaat 
      tcaaaatgt taccacgga gttttggac aatggaacg 
      ggagaatgt gagtgtgat gatggatgg gctactgca 
      acatgcgcc gaaggagcg tatcatccg acactaaat 
      ctttaccat atgtgcaat gtcgggttt aattcttgg 
      caaagggct aatagggaa aagttaata atgacgatt 
      ttagtttta gtgattttg gctattgcc ctcgccgct 
      aaaatgttg ctagcggta tgtctttta aactggtgt 
      tgccacttt atcagatcg agaagtaaa agttatatc 
      gatgatgaa ttctgtgga tcggaacat tgccactat 
      agtagttat tgtggtgac gctgattgt ctccaagag 
      gttcctaaa gataaatct tcaaggaca ataagagtg 
      tccttttca aatattagc tctgctgaa gatactgat 
      gctacagcc agtggagat gacgataaa actcattcc 
      aaatcttca ggaaatgcg agccatgca actattaat 
      ctttgtcac tgtcaaaga taa
    mRNA sequence
    • >TC032588 RA
      acaaaataa tgacagtgt catgtgctg ttacatttc 
      atgtttata ctttcactt tacttttta ttaaatatg 
      aagttatgt tttagtaca gttattata cagaacgcc 
      ggtgtgtac gaaattaac agtgacgat attgcaatg 
      attttttat ttgaccgta gaaaatacg tttgaatgc 
      aaagttcac gtaagtgtt tccagccta aaatgcatt 
      gaaggagat ccttattcc gacacagcg gaccatatg 
      ttcgtaatt gggggagat atgggggtc attaatagc 
      aaaggtgtg aaaaacgct acactaatt tacccaaat 
      ttgtatttg cgaaataga gctgggaaa tgtttaatg 
      tcgctaaat taccaaact gaacaatca tccgagcaa 
      atgatcaga gaaatatat tttaacacg aacgagatt 
      taccataaa ggaggaggt acagccctt gataaatgc 
      tcgacttgt gatctcacg tctgaaact tgtcttcct 
      gtcgactgt gttattaaa tactcctct cgaaggagt 
      tacttcaac cgtgagcgt aaaatttgt cagtcggtt 
      cctgaatgt cccccaaat actatctac gtccccaaa 
      agtaacatt tgcaggaag ttacacgaa ccagtgtgt 
      gagtctgat cttgaaatg attgaggat ggtaacatc 
      gaacattgc cacagtatt tcaaatatt gtgaattca 
      aaatgttac cacggagtt ttggacaat ggaacggga 
      gaatgtgag tgtgatgat ggatgggct actgcaaca 
      tgcgccgaa ggagcgtat catccgaca ctaaatctt 
      taccatatg tgcaatgtc gggtttaat tcttggcaa 
      agggctaat agggaaaag ttaataatg acgatttta 
      gttttagtg attttggct attgccctc gccgctaaa 
      atgttgcta gcggtatgt cttttaaac tggtgttgc 
      cactttatc agatcgaga agtaaaagt tatatcgat 
      gatgaattc tgtggatcg gaacattgc cactatagt 
      agttattgt ggtgacgct gattgtctc caagaggtt 
      cctaaagat aaatcttca aggacaata agagtgtcc 
      ttttcaaat attagctct gctgaagat actgatgct 
      acagccagt ggagatgac gataaaact cattccaaa 
      tcttcagga aatgcgagc catgcaact attaatctt 
      tgtcactgt caaagataa aagaatgac aaataaaac 
      attatgctt tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY