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Gene information
General information
TC002627 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20988373..20995942
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002627
    iBeetle screen iB_08020

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002627 RA
      MTSDKSRIE KIIDQINAS NAKKVALLL VIGFVCYHG 
      ILHVRYGTN SCKWLLSDG RYKGDQEWQ PYGCMLHRY 
      TKIDTRKCL RYIAFYGTS SHFVFIGDK RMQDLYVTF 
      VNHLIQDEE RFLRPPEDI AKNLTHTDN TLKVKAEFF 
      GMPFISKAM IDKFRQWQL SKEPPSVIV AGCALQAIQ 
      ISNASLTMV EEYKVNLTR LVQPIHNLH KKKTKVLWA 
      LQEPVNSEK LKPELRIIT NEIINLYNK AAMEMLHYS 
      EAEIWRSIR LVGEGMISM SPDGIYLPQ RSLLYDIQI 
      LLNMYCNDY MNFNDGTCC SSTETYTTL QIVTFAILG 
      ICASIAIVM LCTRLIMKW RGRPIHEYS QLPDNAHQP 
      PATPGSYYN LFTSLAKMA LIMSYFFLC DRTNFFMKE 
      NKYYSEFSF WLPIGYVTV LGLFFTEDS KYTKVLHRD 
      QLNEWKGWM QLVILVYHI TGASRILPI NMHIKVLIS 
      AYLFLLGYE QFCCVWQRG DIGIVSFFR VLFQLNFIT 
      VTLCLCMNR PYQFYYFVP LLSFWYLMT YCFLAFPPH 
      ITAQTSENN VMQYFYLLI KFVVFFTVI TILFMSEVF 
      FEKVFVTRP WKALFVTTD DDIREWWFR WKLDRYTII 
      YGMGFAVIL LLAQRYNIY DDNNHNNLF SRGLALTGI 
      LVAIAGIGC YLSITFLCS TELECSEIH SYIVFIPIV 
      GYIVLRNIS GVLRTRYSS LFAWFGEIS LELFISQYH 
      IWLAADTHG VLVLIPGYP VLNVMITSF IFVCASHEV 
      HRLTKVLLP YAVPYDWKC VLRNFILFL AVLVPIGIN 
      DGMF
    CDS sequence
    • >TC002627 RA
      atgaccagt gacaaatct cggattgag aaaataatt 
      gatcagatt aatgcaagt aatgccaag aaagtcgct 
      ttactttta gtgatcggt ttcgtgtgt tatcatgga 
      attctccac gtcagatat gggacaaat tcttgcaag 
      tggctgctg tcagacggt cgctacaag ggggaccag 
      gagtggcag ccctacggc tgcatgctc cacagatac 
      accaagatc gataccaga aaatgttta cggtacata 
      gcattttac ggcactagc agccacttt gtgtttatt 
      ggcgataag agaatgcaa gatttgtat gtaactttt 
      gttaaccac ttaattcag gatgaagag cggtttttg 
      cgaccacct gaagatatc gcgaaaaat ctaacacat 
      acggataac acgctgaag gtgaaggct gaatttttt 
      ggcatgcct tttatatca aaagcaatg attgataaa 
      ttcagacag tggcaactg tctaaagaa cccccatct 
      gtgatagta gctggatgc gcattgcaa gccattcaa 
      atatcaaat gcatcactg actatggtt gaagagtat 
      aaagtgaat ttgacgcga cttgttcaa ccgattcat 
      aatcttcac aagaaaaag accaaagtt ttgtgggcg 
      ttacaggaa cctgtaaat tctgagaaa cttaagcct 
      gaactacgt atcatcaca aatgaaatt atcaacttg 
      tataataaa gccgcaatg gagatgtta cattattct 
      gaagccgaa atatggcgt agcatcaga ctggtaggc 
      gagggaatg atctccatg agccctgat ggcatttac 
      ctaccacaa cgttcttta ctatacgac atccaaatt 
      cttctaaac atgtactgc aatgattac atgaacttc 
      aatgacggg acttgctgc agttcgaca gaaacatac 
      acaaccttg cagatagtt acatttgct attcttggc 
      atttgtgca agtatagcc atcgtaatg ctatgcacc 
      cgcctgata atgaaatgg cgcggccgc cccatccac 
      gaatacagc caactcccc gacaacgcc caccaaccc 
      ccagccacc cccggcagc tactacaac ctcttcacc 
      agtctggcc aagatggcc ctcatcatg tcgtacttt 
      ttcctttgt gaccgcacg aatttcttc atgaaagaa 
      aataaatac tattccgaa ttcagcttt tggcttcca 
      atcggctac gttaccgtg ctaggccta tttttcacc 
      gaggacagt aaatacacc aaagtgtta catcgcgac 
      cagctaaac gaatggaaa ggctggatg cagctagtc 
      attctagtg taccacatc acaggcgcg agccgcatc 
      ttgcccata aacatgcac atcaaagtg ttaatatcg 
      gcgtatttg tttctgcta ggctatgag cagttttgc 
      tgcgtctgg cagaggggt gatataggc atagttagc 
      ttcttccgc gtgcttttc cagctcaac ttcataacg 
      gtgacgctg tgtctttgc atgaaccgc ccgtatcag 
      ttctattat ttcgttccg ttgctgtct ttttggtac 
      ttgatgacc tactgcttc ctggcgttt ccgccacac 
      atcacagcg caaacctcc gagaataac gtcatgcag 
      tacttctac ttgctgata aagtttgtt gtcttcttc 
      accgttatt accatcctg ttcatgtcg gaggttttc 
      ttcgagaag gttttcgtc acaaggcct tggaaggcc 
      cttttcgtg acaacagat gacgatatc agggagtgg 
      tggttcagg tggaagctt gacagatac acaataatc 
      tacgggatg ggatttgcc gtaattctg cttttggcg 
      caacgttac aacatttac gatgacaac aaccacaat 
      aatttattt tcgagaggg ttggccttg actgggatt 
      ctagtagca atagcgggg attggctgt tacttgtcg 
      ataaccttc ctctgttcg actgagcta gagtgcagt 
      gagatacac tcctacatt gtttttatt cccattgtg 
      ggctacatt gtgttgagg aacatttcc ggggtgttg 
      cgaactcga tattcgagt ttgtttgct tggtttggc 
      gaaatttca ttggagctt tttataagt cagtatcat 
      atttggctt gcggctgat acgcacggt gttttagta 
      cttatcccg ggttatccg gttttgaat gtaatgatc 
      acgtcgttt atttttgta tgtgcttcg catgaagtg 
      caccgttta accaaagtc ttgctgcca tatgccgtt 
      ccctacgat tggaaatgt gttttgcgg aactttatc 
      ttgtttttg gcggtgttg gtaccgatt gggataaat 
      gatggcatg ttctag
    mRNA sequence
    • >TC002627 RA
      aaaatcaat atggcagtc gattcactg actatttgt 
      tttcccacc taaaattaa ccaacactt taatttgaa 
      atccattgg atcagataa tagataatt attaattag 
      gtttcgaga gacacttgc gataatctc acccacagt 
      tccgttagg gtggtgaca aaaataaac cattccagt 
      ttacactat tctcatgtg acttttctt aacgatgta 
      ggaaaagtg tgaaattat tatgaccag tgacaaatc 
      tcggattga gaaaataat tgatcagat taatgcaag 
      taatgccaa gaaagtcgc tttactttt agtgatcgg 
      tttcgtgtg ttatcatgg aattctcca cgtcagata 
      tgggacaaa ttcttgcaa gtggctgct gtcagacgg 
      tcgctacaa gggggacca ggagtggca gccctacgg 
      ctgcatgct ccacagata caccaagat cgataccag 
      aaaatgttt acggtacat agcatttta cggcactag 
      cagccactt tgtgtttat tggcgataa gagaatgca 
      agatttgta tgtaacttt tgttaacca cttaattca 
      ggatgaaga gcggttttt gcgaccacc tgaagatat 
      cgcgaaaaa tctaacaca tacggataa cacgctgaa 
      ggtgaaggc tgaattttt tggcatgcc ttttatatc 
      aaaagcaat gattgataa attcagaca gtggcaact 
      gtctaaaga acccccatc tgtgatagt agctggatg 
      cgcattgca agccattca aatatcaaa tgcatcact 
      gactatggt tgaagagta taaagtgaa tttgacgcg 
      acttgttca accgattca taatcttca caagaaaaa 
      gaccaaagt tttgtgggc gttacagga acctgtaaa 
      ttctgagaa acttaagcc tgaactacg tatcatcac 
      aaatgaaat tatcaactt gtataataa agccgcaat 
      ggagatgtt acattattc tgaagccga aatatggcg 
      tagcatcag actggtagg cgagggaat gatctccat 
      gagccctga tggcattta cctaccaca acgttcttt 
      actatacga catccaaat tcttctaaa catgtactg 
      caatgatta catgaactt caatgacgg gacttgctg 
      cagttcgac agaaacata cacaacctt gcagatagt 
      tacatttgc tattcttgg catttgtgc aagtatagc 
      catcgtaat gctatgcac ccgcctgat aatgaaatg 
      gcgcggccg ccccatcca cgaatacag ccaactccc 
      cgacaacgc ccaccaacc cccagccac ccccggcag 
      ctactacaa cctcttcac cagtctggc caagatggc 
      cctcatcat gtcgtactt tttcctttg tgaccgcac 
      gaatttctt catgaaaga aaataaata ctattccga 
      attcagctt ttggcttcc aatcggcta cgttaccgt 
      gctaggcct atttttcac cgaggacag taaatacac 
      caaagtgtt acatcgcga ccagctaaa cgaatggaa 
      aggctggat gcagctagt cattctagt gtaccacat 
      cacaggcgc gagccgcat cttgcccat aaacatgca 
      catcaaagt gttaatatc ggcgtattt gtttctgct 
      aggctatga gcagttttg ctgcgtctg gcagagggg 
      tgatatagg catagttag cttcttccg cgtgctttt 
      ccagctcaa cttcataac ggtgacgct gtgtctttg 
      catgaaccg cccgtatca gttctatta tttcgttcc 
      gttgctgtc tttttggta cttgatgac ctactgctt 
      cctggcgtt tccgccaca catcacagc gcaaacctc 
      cgagaataa cgtcatgca gtacttcta cttgctgat 
      aaagtttgt tgtcttctt caccgttat taccatcct 
      gttcatgtc ggaggtttt cttcgagaa ggttttcgt 
      cacaaggcc ttggaaggc ccttttcgt gacaacaga 
      tgacgatat cagggagtg gtggttcag gtggaagct 
      tgacagata cacaataat ctacgggat gggatttgc 
      cgtaattct gcttttggc gcaacgtta caacattta 
      cgatgacaa caaccacaa taatttatt ttcgagagg 
      gttggcctt gactgggat tctagtagc aatagcggg 
      gattggctg ttacttgtc gataacctt cctctgttc 
      gactgagct agagtgcag tgagataca ctcctacat 
      tgtttttat tcccattgt gggctacat tgtgttgag 
      gaacatttc cggggtgtt gcgaactcg atattcgag 
      tttgtttgc ttggtttgg cgaaatttc attggagct 
      ttttataag tcagtatca tatttggct tgcggctga 
      tacgcacgg tgttttagt acttatccc gggttatcc 
      ggttttgaa tgtaatgat cacgtcgtt tatttttgt 
      atgtgcttc gcatgaagt gcaccgttt aaccaaagt 
      cttgctgcc atatgccgt tccctacga ttggaaatg 
      tgttttgcg gaactttat cttgttttt ggcggtgtt 
      ggtaccgat tgggataaa tgatggcat gttctaggg 
      tatagtcgg aatagaaaa gaactgttg ttatttttg 
      aaaagcgca cattgtaat gtacatacg tttttatat 
      aataacatt gtatgtagg ttttcgtaa ataaagaaa 
      ttttttata a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY