Status
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Gene information
General information
TC032541
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002662
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17585215..17587487
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032541
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08018

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032541 RA
      MEFIVMPPS LFSKIDANY RNFTALDVD ALKNLPKVK 
      KLKLDRLEI PHLTHDYLK NLTSVTNLT LQHCEIEEI 
      DPHVFSDLK NLEEVGLNC NKLTKLPQN LFKNNPKLE 
      KLTLIWNRI SNLTWDEFE GLDTLKVLY IGQNSIKHF 
      DAEKISKNM PNLRELNVF LTKLKGSEL EKFQRELQA 
      KLNQSPCKC LRGSFESIF LLILVVNLQ LLSLWKDCL 
      PEISDIEPR KEPLQINEA YTQVKLDNV TGTLYATSL 
      SKLSKITDL QIYTNGLED VEDGALEIN TSDDELYLK 
      MWGDLYSDS FSKLDNITH ISIFANNLT EIQPGAICS 
      NPALRKMEF TVMPPSLFS KLTKSHLEN CQNLEELEF 
      YGSYWNFTA LDVDALENL PKVKKLKLD RLEIPHLTH 
      DYLKNLTSV TNLTLQHCE IEEIDPDVF SDLKNLEEV 
      GLNCNKLTK LPQNLFKNN PKLEKLTLV SNRIRNLTW 
      DEFEGLDTL KVLYIGQNS IKHFDAEKI AKNMPNLRE 
      LNVFLTKLK GSELEEFQR ELQAKLNHT VNVSGEYDH 
      RSYSLCV
    CDS sequence
    • >TC032541 RA
      atggagttt atcgtaatg ccaccatca cttttttcg 
      aaaattgac gcaaactac aggaatttc actgccctc 
      gacgtagac gctcttaag aatttacca aaagtaaag 
      aaactcaag cttgataga ttagaaatt ccacacttg 
      actcacgat tatttgaaa aacctcacc tctgtgact 
      aatctaact ctacagcat tgtgaaatt gaagaaatc 
      gatccacac gtgttcagt gatttgaaa aaccttgaa 
      gaagttgga ttgaattgc aacaaattg acaaaactg 
      ccgcaaaat ttattcaaa aataatccc aaattagaa 
      aaactgact ttgatttgg aatagaata agtaacttg 
      acttgggac gagtttgag ggattggac actttgaag 
      gtgctttac attggacaa aactcgatc aagcatttt 
      gatgccgaa aaaatttcc aaaaatatg cccaacctg 
      agggaactc aacgtgttt ttgaccaaa ctgaagggc 
      tccgaattg gagaaattt caaagggaa ctgcaagca 
      aaactgaat caatcaccc tgtaaatgt ctcagggga 
      tcttttgaa agcattttc ttgcttatt ttggtagta 
      aatcttcaa ttattaagt ctttggaag gactgtttg 
      cctgaaatt tctgatata gagccaagg aaagaacca 
      cttcaaatt aatgaagct tacactcaa gtcaagctt 
      gataacgtg accggaacc ctttacgca acttcctta 
      tcaaaatta tcaaaaata acggatctt cagatttac 
      accaacggg ttagaagat gtggaagac ggtgcttta 
      gaaataaac acatctgat gacgagtta tatttgaag 
      atgtggggt gacctatat tccgattca ttttcaaag 
      ttggataat ataacacat atttcgatt tttgcaaac 
      aatttaact gaaatacaa ccaggagca atttgttcc 
      aatccagct ttaagaaaa atggaattt accgtaatg 
      ccaccatca cttttttca aaattgacg aaaagtcac 
      ttggaaaat tgccaaaat ttggaagaa ctcgaattc 
      tatggtagc tactggaat ttcactgcc ctcgacgta 
      gacgctctt gaaaattta ccaaaagta aagaaactc 
      aaacttgat agattagaa attccacac ttgactcac 
      gattatttg aaaaacctc acctctgtg actaatcta 
      actctacag cattgtgaa attgaagaa attgatcca 
      gacgtgttc agtgatttg aaaaacctt gaagaagtt 
      ggattgaat tgcaacaaa ttgacaaaa ctgccgcaa 
      aatctattc aaaaataat cccaaatta gaaaaactg 
      accttggtt tcgaataga ataagaaac ttgacttgg 
      gacgagttt gagggattg gacactttg aaggtgctt 
      tacattgga caaaactcg atcaagcat tttgatgcc 
      gaaaaaatt gccaaaaat atgcccaac ctgagggaa 
      ctcaacgtg tttttgacc aaactgaag ggctccgaa 
      ttggaggaa tttcaaagg gaactgcaa gcaaaactg 
      aatcacact gtaaatgtc tcaggggag tatgaccac 
      aggtcttat tccctctgt gtttga
    mRNA sequence
    • >TC032541 RA
      aacaccaaa aacgtaatt tttgaattt ttagaagcc 
      gtttttatt gattttgtt tctaataaa cgccaattg 
      tttggatga tatttggga tgtgaaaag tctaaatat 
      tataataac tgaaggagg tcctttaga aataaacac 
      atctgatga cgagttata tttgaagat gtggggtga 
      cctatattc cgattcatt ttcaaagtt ggataatat 
      aacatattt cgatttttg caaacaatt taactgaaa 
      tacaaccag gagctattt gttccaatc tagctttaa 
      gaaaaatgg agtttatcg taatgccac catcacttt 
      tttcgaaaa ttgacgcaa actacagga atttcactg 
      ccctcgacg tagacgctc ttaagaatt taccaaaag 
      taaagaaac tcaagcttg atagattag aaattccac 
      acttgactc acgattatt tgaaaaacc tcacctctg 
      tgactaatc taactctac agcattgtg aaattgaag 
      aaatcgatc cacacgtgt tcagtgatt tgaaaaacc 
      ttgaagaag ttggattga attgcaaca aattgacaa 
      aactgccgc aaaatttat tcaaaaata atcccaaat 
      tagaaaaac tgactttga tttggaata gaataagta 
      acttgactt gggacgagt ttgagggat tggacactt 
      tgaaggtgc tttacattg gacaaaact cgatcaagc 
      attttgatg ccgaaaaaa tttccaaaa atatgccca 
      acctgaggg aactcaacg tgtttttga ccaaactga 
      agggctccg aattggaga aatttcaaa gggaactgc 
      aagcaaaac tgaatcaat caccctgta aatgtctca 
      ggggatctt ttgaaagca ttttcttgc ttattttgg 
      tagtaaatc ttcaattat taagtcttt ggaaggact 
      gtttgcctg aaatttctg atatagagc caaggaaag 
      aaccacttc aaattaatg aagcttaca ctcaagtca 
      agcttgata acgtgaccg gaacccttt acgcaactt 
      ccttatcaa aattatcaa aaataacgg atcttcaga 
      tttacacca acgggttag aagatgtgg aagacggtg 
      ctttagaaa taaacacat ctgatgacg agttatatt 
      tgaagatgt ggggtgacc tatattccg attcatttt 
      caaagttgg ataatataa cacatattt cgatttttg 
      caaacaatt taactgaaa tacaaccag gagcaattt 
      gttccaatc cagctttaa gaaaaatgg aatttaccg 
      taatgccac catcacttt tttcaaaat tgacgaaaa 
      gtcacttgg aaaattgcc aaaatttgg aagaactcg 
      aattctatg gtagctact ggaatttca ctgccctcg 
      acgtagacg ctcttgaaa atttaccaa aagtaaaga 
      aactcaaac ttgatagat tagaaattc cacacttga 
      ctcacgatt atttgaaaa acctcacct ctgtgacta 
      atctaactc tacagcatt gtgaaattg aagaaattg 
      atccagacg tgttcagtg atttgaaaa accttgaag 
      aagttggat tgaattgca acaaattga caaaactgc 
      cgcaaaatc tattcaaaa ataatccca aattagaaa 
      aactgacct tggtttcga atagaataa gaaacttga 
      cttgggacg agtttgagg gattggaca ctttgaagg 
      tgctttaca ttggacaaa actcgatca agcattttg 
      atgccgaaa aaattgcca aaaatatgc ccaacctga 
      gggaactca acgtgtttt tgaccaaac tgaagggct 
      ccgaattgg aggaatttc aaagggaac tgcaagcaa 
      aactgaatc acactgtaa atgtctcag gggagtatg 
      accacaggt cttattccc tctgtgttt gaaaaaata 
      tgttttcat tgttgactt gtatcgaat aacgataaa 
      taaatcgat aacctctta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY