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Gene information
General information
TC002683 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18418980..18428548
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002683
    iBeetle screen iB_08015

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002683 RA
      MSKSPITVQ QWVESLPPP HSNTNIYTS 
      PLRNTRKFL PREISLQSD DASSHCSSV 
      ESVLELRKP DPEAVLLGL GFGPPKNTN 
      MVSRIPSRF LQPSKIDID KFLEQYGEA 
      KCSNQHTAP GSPARSQPK SC
    CDS sequence
    • >TC002683 RA
      atgagcaaa tctccgata accgttcag caatgggtc 
      gaatcactg ccaccgcct catagcaac acaaatatt 
      tacacttcg ccattgaga aatactcga aaatttctt 
      ccaagagag atttccctc caatcggac gatgctagt 
      agtcattgt tccagtgtt gaatcggtg ctagagctc 
      cggaagccc gaccctgag gcggtgctc ctaggtttg 
      gggtttggg cccccaaaa aacacaaac atggtgtcg 
      agaatacca agcaggttt ctacaacct tccaagatt 
      gatatcgac aaatttttg gagcaatac ggagaagca 
      aaatgctcg aaccaacac acagctcca ggatctccc 
      gccaggtca caaccaaaa tcttgctaa
    mRNA sequence
    • >TC002683 RA
      agattccct cccgtattc tgttaggtc ccgtttttg 
      tcccgtatt tcagtgtgc tagcaagga aaagtattt 
      ttaaaagaa acacaaaca caattttgt gtacagtga 
      aaaaaacac tgaccaagc tttcagcat cgttcattt 
      tttcctcgt cattttggt tgataagag ctggtccat 
      cctgcggtg ccctttagg tgtagccac atttttcgc 
      acattttag cacaaacac atcacgaaa aataacgag 
      actgtcgaa ctgtaaaca tctgtcaga tttgacaag 
      tgatgtcaa agtggagtt tcaaatttg aatcagtgc 
      gcgcccgac tgataacaa gtaacaaca cgaaacgta 
      cgcacttga actgacact ttcgcggga tgtaatatt 
      ataaaatct cttatcata aagaaaata aaacgaagt 
      ctttctcac cgctttcac caacgtgac gtcattttt 
      aaatgtttt ttcacgcgc tgccaacct aaccttgcc 
      gaagtcccc acgtcactg tcattaaaa tgtcaacaa 
      gtgagtgat ctagcccga tttaatgaa tgggcttaa 
      tctagaaga tattaattg tgattgttg cgttcatta 
      tcagattag agttttgtg cggtattta ttggccact 
      catccgttt gattcgaaa ttcactgtt aatgtgtta 
      actacttgt tgattccgt catttcgtc cagtttttc 
      gagactttg gtctttaaa aaatgagca aatctccga 
      taaccgttc agcaatggg tcgaatcac tgccaccgc 
      ctcatagca acacaaata tttacactt cgccattga 
      gaaatactc gaaaatttc ttccaagag agatttccc 
      tccaatcgg acgatgcta gtagtcatt gttccagtg 
      ttgaatcgg tgctagagc tccggaagc ccgaccctg 
      aggcggtgc tcctaggtt tggggtttg ggcccccaa 
      aaaacacaa acatggtgt cgagaatac caagcaggt 
      ttctacaac cttccaaga ttgatatcg acaaatttt 
      tggagcaat acggagaag caaaatgct cgaaccaac 
      acacagctc caggatctc ccgccaggt cacaaccaa 
      aatcttgct aatttattc cattttgtt acattagtg 
      ttagtagac caaaatatt ttttaaaca ttgagatcg 
      atcttcaac tgtccttgg aattgaata attattcga 
      attgattag atgtaggat aaacataac aaaaatgtg 
      atctaaaaa actgtatta taaaacatt tacatttaa 
      atgaatgaa aaaaattcg gacctaaat accatgtta 
      aaaaaccaa tttccattg tatcatgcc aaggacatt 
      gttttcaca ttgtcaacg gaatgtggc ttacacatt 
      attttcgat cgtttcaac acatacaca atactcaaa 
      aatgccgca actttaaaa atgtaccga gggagcgtt 
      aacttgaaa aaaattcac cgaaatgcc aatagttaa 
      tagtaatta ttaattcca aattacaag aaaaattcc 
      aaaaattcg tccaattcc agagttaac gcccgtcga 
      aatacataa acatgtctg taaaaagaa atgtaagag 
      cattctgtt gacagttcc tcattccta ctattcaaa 
      gggtaacgt tctctagtg tgctttatt ttttattct 
      ctttataat aaacatgtg atgtaaaat gtgattaag 
      ttgtatagg aagacaaat cattatatt aggggtaag 
      catatcgga actaacgtc aattatgtc atagttgcg 
      ttttttaaa caacactgg taccaaaaa accgtattt 
      ttagcaatt tgaaatgtt gtgaatcct caaagtgca 
      acacttgcc attgtttcc ttttgcggg ttgtattta 
      ctgtcgatt ttgttgcca tgatgtaaa ttgtgtttc 
      aaaaatttc ttccaaatc gccgaattc caaattttt 
      gaagcacaa tatttgttt aatgttggc ttcctttat 
      tgttagttg ttaagggta ggatcatca tgatcaaat 
      tccactggc tttaatatt gtttatatt tttgtggac 
      caatttttg tgaaataaa aattgttga cgtcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY