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Gene information
General information
TC002720 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13372006..13373213
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002720
    iBeetle screen iB_08008

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002720 RA
      MVSNGSNEA FIRKNKVVT VDEDENTNL HYAAALDNL 
      DKIEKIVAK KQNIDPENA LGWTPLMMA VRNSNLKAV 
      GLLLSHGAD PRKQNKFGM NVFLMSVAS GHLDMVERL 
      LKHLLWEGT SRQKLQKTL SPLSLALLF GHDHIFKYL 
      LERRFDPNA ATPLTLITP AMFAAALAN NKATKKLLR 
      FKADLTLKN YGGKTASDI AQAKQKFCI APKKQEQQP 
      LIAMSPHTA NSPVFSNFE NFAGRGRLL RNSSNITPN 
      PSIPMLCAN VAPIAPVVP HMMPQMIFP PKFLPNNFT 
      SPMGVFHSN FGFLNVAMI PPVTYFSPY LLSQMSS
    CDS sequence
    • >TC002720 RA
      atggtctca aacggctca aacgaagcg ttcatacga 
      aaaaataag gtggttaca gtcgacgag gatgaaaac 
      acgaactta cattacgct gcggctttg gataattta 
      gacaaaatc gagaaaata gtggcgaaa aaacagaat 
      attgatcca gaaaatgct cttggatgg acaccattg 
      atgatggcg gtcagaaat tcaaatttg aaggccgtg 
      ggtttgctt ttgagtcac ggagcagat ccgaggaaa 
      caaaataag tttggcatg aatgtattt ttaatgagc 
      gtggcaagt ggccacctt gacatggtc gagcgtctt 
      ttaaaacac ctgttatgg gagggaaca tcaagacaa 
      aaattgcaa aaaactctc tcgcctcta tctttggct 
      cttttgttc ggacacgac cacatcttc aagtatttg 
      ctggagcgc cgttttgac ccgaacgca gcaacacct 
      ctgacatta atcacccct gcgatgttt gccgccgct 
      ttagccaac aacaaggca actaaaaag ctattgcga 
      tttaaggct gacctaaca ttgaaaaac tatgggggg 
      aaaacggcc tcagatatt gctcaagca aaacaaaaa 
      ttttgtatt gctccaaag aaacaagaa caacaaccg 
      ttaattgca atgagtcct catactgca aattctcca 
      gttttttcc aattttgag aattttgca ggtagggga 
      agacttctg aggaattct tcaaatatt actccaaat 
      cctagtatt cctatgctg tgtgctaat gttgctcca 
      attgctcca gtggtgcct cacatgatg ccacagatg 
      atttttcca ccgaaattt ttacccaac aattttaca 
      agtccgatg ggagttttt cattcgaat ttcggcttt 
      ttaaacgtt gctatgatc ccgccagtt acgtatttc 
      tcgccttat cttttatca caaatgtct tcgtaa
    mRNA sequence
    • >TC002720 RA
      atcatttta ggtccttct accttacac cactttatt 
      actctacca aaaaatcct acaaagaat aaaagaagt 
      cagcattga aatggtctc aaacggctc aaacgaagc 
      gttcatacg aaaaaataa ggtggttac agtcgacga 
      ggatgaaaa cacgaactt acattacgc tgcggcttt 
      ggataattt agacaaaat cgagaaaat agtggcgaa 
      aaaacagaa tattgatcc agaaaatgc tcttggatg 
      gacaccatt gatgatggc ggtcagaaa ttcaaattt 
      gaaggccgt gggtttgct tttgagtca cggagcaga 
      tccgaggaa acaaaataa gtttggcat gaatgtatt 
      tttaatgag cgtggcaag tggccacct tgacatggt 
      cgagcgtct tttaaaaca cctgttatg ggagggaac 
      atcaagaca aaaattgca aaaaactct ctcgcctct 
      atctttggc tcttttgtt cggacacga ccacatctt 
      caagtattt gctggagcg ccgttttga cccgaacgc 
      agcaacacc tctgacatt aatcacccc tgcgatgtt 
      tgccgccgc tttagccaa caacaaggc aactaaaaa 
      gctattgcg atttaaggc tgacctaac attgaaaaa 
      ctatggggg gaaaacggc ctcagatat tgctcaagc 
      aaaacaaaa attttgtat tgctccaaa gaaacaaga 
      acaacaacc gttaattgc aatgagtcc tcatactgc 
      aaattctcc agttttttc caattttga gaattttgc 
      aggtagggg aagacttct gaggaattc ttcaaatat 
      tactccaaa tcctagtat tcctatgct gtgtgctaa 
      tgttgctcc aattgctcc agtggtgcc tcacatgat 
      gccacagat gatttttcc accgaaatt tttacccaa 
      caattttac aagtccgat gggagtttt tcattcgaa 
      tttcggctt tttaaacgt tgctatgat cccgccagt 
      tacgtattt ctcgcctta tcttttatc acaaatgtc 
      ttcgtaata tgtttaagt tttatgtaa tacagggtg 
      acccagggg tgcgtgtat gctccata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY