Status
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Gene information
General information
TC001642 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450448.1: 4495..9783
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001642
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08007

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001642 RA
      MRRSGGESC EDFGAMEVP QDSLFRIFL KVRSLQPPI 
      VEILLNELT TTTAVDTFD PGHLRLLLK QLPYLPYIK 
      TPETLTTKL LDIIDIAPY GAQLELLHS IPDSEHHTV 
      VKELAKMLD NTNISGPII DCLNALNLT PDMRTHVQD 
      HILSKMLAT ISTKLFPVY LDFLLTDCN SKTLPRILP 
      KLRHALNDI MSRDEDAES KISIFTKLQ KWANLKSVS 
      EAWLSLIAN TKDHKYIDL LLLSMLHAR KKRTIESTI 
      RKKIKGGQF KSLQVEQLF ARDFPQQVF RDYFGTFCE 
      VGSSLLRTS TAPTIVEFA STLFKLLFT HKRTVRIYE 
      VLENLIVLV GCTDNKTAV LNIVNDLVP QILQAPNHA 
      TILMRLLEI DSFELKDIK LVFEILCKV TCSDDSLIG 
      LREEIHMLV RKQMSNFKQ SAKERGIIS AVVLAKNIA 
      NVSGDQSDV LSEESIASI GQLTGGAKD AANLLTVVQ 
      SSTVNTPDL VSLYYDQLA AMISNSDGL DKRFLAWLH 
      ERITTDFQN FYITETTDT TIGDLEVTK QYDLNGDEE 
      MDAPISINI AELTLRPGK HSIVALAPH FRLLRLVHF 
      RQQNGDLDT IDALLGCGV ILPKQLQTT DCLLHCINW 
      FREIISGFV LQKREKLRR KVLQRIDHL IELEQLFSE 
      SLDSIPKKL KKNNRERET VLDTTFREM DNDLVTLLK 
      YPLEATNQV SLSVKQLNF ILGDYVRKL SLETKGGDL 
      GLSHLGAVS PLHVITDSV KTLPHIKRH LAAVLPECH 
      TNDEAKATF GLVVECLCL IFNWPGLQH AKRLVLVKD 
      IIKAFRSGD SELHSANHL VTELVEQLA DLTQFCTCL 
      THAVHFIQT MEALDFRSY LSSASVKTL CGIVGTVQE 
      QAPGLTKSE PLEMLKSVT KTNFFVFYR NLWACLHDR 
      VKSETNAQH VATWRTIAL TMQGLMAVA KAQKTKPNL 
      VCCLKKSTA ILKLFLTHG IPILEIEFK AKSELVIEI 
      FKTMHSNAR FLHHLCCYS KATKDVGLL SHVPQFRLT 
      FETLVYRVK AALAANGCS AAFWMGNLR NRDLDGEDI 
      LSESSDNSS HEGGGEEEG EVRGYRVMM SRLGISRRV 
      RFLSNLLSR FGWGLL
    CDS sequence
    • >TC001642 RA
      atgaggcgc agtgggggt gagagttgt gaggatttt 
      ggggcgatg gaagtgcca caagacagt ctatttcgg 
      atattcctg aaggtgcgt tccctccag ccacccatc 
      gtggaaatc ctcctcaac gagctcacc acgaccaca 
      gccgtggac accttcgac ccaggtcac ctccgtctc 
      ctcctcaaa caactcccc tacctcccc tacatcaaa 
      actcccgaa acgctcaca accaaactc ctcgacatc 
      atcgacata gctccatac ggcgcccaa ctcgaactc 
      ctccactcc atccccgac agcgaacac cacacagtc 
      gtcaaagaa ctcgccaaa atgctcgac aataccaac 
      atctccggg cccataatc gactgtctt aacgcttta 
      aacctaacc cccgacatg cgtacgcac gtacaagac 
      cacatcttg tccaaaatg ttggccacg atctccacc 
      aaactattc ccagtgtac ttggacttt ttactaacc 
      gactgcaac agcaagact ttaccccgg atcttgccc 
      aagcttcga cacgcttta aacgacatc atgtcacgt 
      gatgaagac gccgaatca aaaatctcc attttcaca 
      aaactacaa aaatgggcg aatttgaaa tcagtctcc 
      gaagcgtgg ttaagtctc attgcaaac acgaaagac 
      cacaaatac atcgacttg ctactcttg tccatgcta 
      catgcccgc aaaaaacgg acgattgaa agcacaatt 
      cgcaaaaaa atcaaaggc ggccagttc aagtcactc 
      caagtggag caacttttc gcccgggat ttcccccaa 
      caagtgttc cgcgactat tttggtacg ttttgcgaa 
      gttgggtca agtctccta cgcacgtca accgctcca 
      acaatcgtg gaatttgca tcgacactc ttcaaactc 
      ctcttcacg cacaagcgc accgttcgg atctacgaa 
      gtgcttgaa aatctgatc gtcttggtt ggctgcact 
      gacaataag acagcggtc ctcaacatt gtcaatgac 
      ttggttccc caaatcctc caagcgcca aaccatgcc 
      acgattttg atgcgtctc ctggagatt gattcgttc 
      gaactgaag gacattaag ctcgtgttt gagattttg 
      tgcaaagtg acgtgttcg gatgattct ttaatcggc 
      ctacgcgag gaaatacac atgttggtg cgcaaacaa 
      atgtccaat tttaaacaa agtgccaaa gaacgcggc 
      atcatatcg gccgtggtt ttggccaag aacatagct 
      aatgttagt ggtgaccaa agtgatgtg ttgtccgaa 
      gagtcaatt gccagtatt ggacaattg acaggtggg 
      gcgaaggac gcagctaac ttactcact gtggttcag 
      agctccacg gttaatacg cctgatcta gtcagtctg 
      tattacgac cagttggca gcaatgatt tccaatagt 
      gatgggtta gataagcgc ttcctggcc tggctccac 
      gaacgcata accaccgac ttccagaat ttctacatc 
      acggaaacc accgacacg actattggt gatttggaa 
      gtgaccaaa caatacgat ctgaatggt gacgaagaa 
      atggatgcg ccaatttca attaacatt gctgaatta 
      accctcagg ccgggcaaa cactcgatc gttgcccta 
      gcgccccat ttccgactg ttgcgtttg gtgcacttt 
      cggcaacaa aacggcgac ttggacacg atcgatgcc 
      ttgctaggc tgtggggtc attctacca aaacaattg 
      caaacaacc gactgtctt ttgcactgt atcaactgg 
      ttccgggag ataattagt ggtttcgtc ctccagaaa 
      cgtgagaaa ttgagacgc aaagtgctc caaagaatc 
      gatcatttg atcgagttg gaacaactg ttttccgag 
      tcacttgac tcaatcccc aaaaaactg aaaaaaaac 
      aaccgggaa cgggaaact gtccttgac acaaccttc 
      cgggaaatg gacaacgat ctagtgaca ctcctcaaa 
      taccctctc gaggcaaca aatcaagtt agcctgagc 
      gtgaaacaa ctgaacttc attttgggg gattacgtg 
      cgcaagctg tcacttgag acgaaaggg ggcgacctc 
      ggtttgtca cacttgggg gcggtcagc cccttgcat 
      gtgataacc gattcggtt aaaaccctt ccgcacatc 
      aagcgacac ttggcggcg gttttgccc gaatgtcac 
      accaatgac gaggcgaaa gccaccttc ggattggtt 
      gtggaatgt ttgtgtttg atttttaat tggcccggc 
      ttacaacac gccaaacgt ctcgtccta gttaaagac 
      atcatcaag gcgttccgt tcgggtgac agtgagctg 
      cactcggcc aatcacctc gtcaccgaa ctggtggaa 
      caactagcc gacttgacg caattttgc acgtgtcta 
      acacacgca gtccacttc attcaaaca atggaagcc 
      ctcgatttc cgctcatat ctgagcagt gctagtgtt 
      aaaaccctg tgtgggatt gttggtacg gtccaggag 
      caagcccca ggtctcact aagagtgaa ccgttggag 
      atgctcaaa agtgtgact aagaccaac ttttttgtt 
      ttctaccgc aatttatgg gcgtgtttg cacgatcgt 
      gtcaaaagc gagacgaat gcgcaacat gtggcgacc 
      tggcgcacc attgccctc acaatgcaa ggcttgatg 
      gcggtggcc aaggcacag aaaaccaaa ccgaacctg 
      gtctgttgc cttaagaag tccaccgcg attttaaag 
      cttttcctc acgcatgga atcccaatc ttggagatt 
      gagttcaaa gcaaagtcg gagcttgtg attgagatt 
      ttcaaaacc atgcattca aatgcacgg tttttgcac 
      catttgtgt tgctattct aaagcgact aaagacgtt 
      ggtttatta tcgcacgtg ccgcagttt cggttaact 
      ttcgagacg ctggtgtac cgggttaag gccgctttg 
      gcggcaaat gggtgttcg gcggcgttc tggatgggg 
      aatttgagg aatcgggac ttggatggg gaggatatt 
      ttgagtgag agtagcgat aattcgagt catgaggga 
      gggggtgag gaggagggg gaagtgagg ggttaccga 
      gtgatgatg agtcggttg gggataagt cgtcgagtg 
      aggtttttg agtaactta ctttctcgg tttggatgg 
      ggtttgtta tga
    mRNA sequence
    • >TC001642 RA
      agagattta tcaagggtt ttaatattt tgatcagga 
      aggagaaat atttgaaga aggctttac tgcccactg 
      tttttcgga tgaggcgca gtgggggtg agagttgtg 
      aggattttg gggcgatgg aagtgccac aagacagtc 
      tatttcgga tattcctga aggtgcgtt ccctccagc 
      cacccatcg tggaaatcc tcctcaacg agctcacca 
      cgaccacag ccgtggaca ccttcgacc caggtcacc 
      tccgtctcc tcctcaaac aactcccct acctcccct 
      acatcaaaa ctcccgaaa cgctcacaa ccaaactcc 
      tcgacatca tcgacatag ctccatacg gcgcccaac 
      tcgaactcc tccactcca tccccgaca gcgaacacc 
      acacagtcg tcaaagaac tcgccaaaa tgctcgaca 
      ataccaaca tctccgggc ccataatcg actgtctta 
      acgctttaa acctaaccc ccgacatgc gtacgcacg 
      tacaagacc acatcttgt ccaaaatgt tggccacga 
      tctccacca aactattcc cagtgtact tggactttt 
      tactaaccg actgcaaca gcaagactt taccccgga 
      tcttgccca agcttcgac acgctttaa acgacatca 
      tgtcacgtg atgaagacg ccgaatcaa aaatctcca 
      ttttcacaa aactacaaa aatgggcga atttgaaat 
      cagtctccg aagcgtggt taagtctca ttgcaaaca 
      cgaaagacc acaaataca tcgacttgc tactcttgt 
      ccatgctac atgcccgca aaaaacgga cgattgaaa 
      gcacaattc gcaaaaaaa tcaaaggcg gccagttca 
      agtcactcc aagtggagc aacttttcg cccgggatt 
      tcccccaac aagtgttcc gcgactatt ttggtacgt 
      tttgcgaag ttgggtcaa gtctcctac gcacgtcaa 
      ccgctccaa caatcgtgg aatttgcat cgacactct 
      tcaaactcc tcttcacgc acaagcgca ccgttcgga 
      tctacgaag tgcttgaaa atctgatcg tcttggttg 
      gctgcactg acaataaga cagcggtcc tcaacattg 
      tcaatgact tggttcccc aaatcctcc aagcgccaa 
      accatgcca cgattttga tgcgtctcc tggagattg 
      attcgttcg aactgaagg acattaagc tcgtgtttg 
      agattttgt gcaaagtga cgtgttcgg atgattctt 
      taatcggcc tacgcgagg aaatacaca tgttggtgc 
      gcaaacaaa tgtccaatt ttaaacaaa gtgccaaag 
      aacgcggca tcatatcgg ccgtggttt tggccaaga 
      acatagcta atgttagtg gtgaccaaa gtgatgtgt 
      tgtccgaag agtcaattg ccagtattg gacaattga 
      caggtgggg cgaaggacg cagctaact tactcactg 
      tggttcaga gctccacgg ttaatacgc ctgatctag 
      tcagtctgt attacgacc agttggcag caatgattt 
      ccaatagtg atgggttag ataagcgct tcctggcct 
      ggctccacg aacgcataa ccaccgact tccagaatt 
      tctacatca cggaaacca ccgacacga ctattggtg 
      atttggaag tgaccaaac aatacgatc tgaatggtg 
      acgaagaaa tggatgcgc caatttcaa ttaacattg 
      ctgaattaa ccctcaggc cgggcaaac actcgatcg 
      ttgccctag cgccccatt tccgactgt tgcgtttgg 
      tgcactttc ggcaacaaa acggcgact tggacacga 
      tcgatgcct tgctaggct gtggggtca ttctaccaa 
      aacaattgc aaacaaccg actgtcttt tgcactgta 
      tcaactggt tccgggaga taattagtg gtttcgtcc 
      tccagaaac gtgagaaat tgagacgca aagtgctcc 
      aaagaatcg atcatttga tcgagttgg aacaactgt 
      tttccgagt cacttgact caatcccca aaaaactga 
      aaaaaaaca accgggaac gggaaactg tccttgaca 
      caaccttcc gggaaatgg acaacgatc tagtgacac 
      tcctcaaat accctctcg aggcaacaa atcaagtta 
      gcctgagcg tgaaacaac tgaacttca ttttggggg 
      attacgtgc gcaagctgt cacttgaga cgaaagggg 
      gcgacctcg gtttgtcac acttggggg cggtcagcc 
      ccttgcatg tgataaccg attcggtta aaacccttc 
      cgcacatca agcgacact tggcggcgg ttttgcccg 
      aatgtcaca ccaatgacg aggcgaaag ccaccttcg 
      gattggttg tggaatgtt tgtgtttga tttttaatt 
      ggcccggct tacaacacg ccaaacgtc tcgtcctag 
      ttaaagaca tcatcaagg cgttccgtt cgggtgaca 
      gtgagctgc actcggcca atcacctcg tcaccgaac 
      tggtggaac aactagccg acttgacgc aattttgca 
      cgtgtctaa cacacgcag tccacttca ttcaaacaa 
      tggaagccc tcgatttcc gctcatatc tgagcagtg 
      ctagtgtta aaaccctgt gtgggattg ttggtacgg 
      tccaggagc aagccccag gtctcacta agagtgaac 
      cgttggaga tgctcaaaa gtgtgacta agaccaact 
      tttttgttt tctaccgca atttatggg cgtgtttgc 
      acgatcgtg tcaaaagcg agacgaatg cgcaacatg 
      tggcgacct ggcgcacca ttgccctca caatgcaag 
      gcttgatgg cggtggcca aggcacaga aaaccaaac 
      cgaacctgg tctgttgcc ttaagaagt ccaccgcga 
      ttttaaagc ttttcctca cgcatggaa tcccaatct 
      tggagattg agttcaaag caaagtcgg agcttgtga 
      ttgagattt tcaaaacca tgcattcaa atgcacggt 
      ttttgcacc atttgtgtt gctattcta aagcgacta 
      aagacgttg gtttattat cgcacgtgc cgcagtttc 
      ggttaactt tcgagacgc tggtgtacc gggttaagg 
      ccgctttgg cggcaaatg ggtgttcgg cggcgttct 
      ggatgggga atttgagga atcgggact tggatgggg 
      aggatattt tgagtgaga gtagcgata attcgagtc 
      atgagggag ggggtgagg aggaggggg aagtgaggg 
      gttaccgag tgatgatga gtcggttgg ggataagtc 
      gtcgagtga ggtttttga gtaacttac tttctcggt 
      ttggatggg gtttgttat gatgccctc tttgatgaa 
      aaggccata gtcggcgta aaacttctc gtattcgtc 
      tggtttttt gttaagttg gtctagcag gaacttcac 
      gacacggct agtcaggac gtctcggag ttttctgca 
      aatttatat aatctcatt tttttgtaa ttttctaaa 
      tatattaat tgttaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY