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Gene information
General information
TC002742 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12767313..12769683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002742
    iBeetle screen iB_08003

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    No phenotype observable.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002742 RA
      MPAMKDSYI VKIAVEMTD QVPQLIEFN QKQPLAGII 
      QELCNGWNL SDPEQYALQ FNDNNNRNY ITEKNRNEI 
      KNGSVLRLQ YSPSKTAQD ILHKLHNGN AEEKAHAMH 
      RLSNLSADM TFALDFINK QGLTLIIKQ IETGKFKGA 
      VLAHTLLSF VELMEHGIV SWDILNGDF ITRVASFVN 
      SPQEAEVTQ AALSILENV VLNSTEGYG QVEREIPIG 
      SLVGHLQAT PVVQQNAIA LINALLLRA DSVKRRSLA 
      ATLTTKQVR SMIQNHILQ TGAALGAEM AHQLYVLQT 
      LMLGLLEQR MVTKMDPQD QDGHDKIKE LRRIAFDSE 
      GAGGGRGPG GFTRDYKKL GFKNDINPA LDFTETPPG 
      LLALDCMIY FARNHPDSY TKLVLENSC RADEHECPF 
      GRASVELVR ILCELLKIG DAPSEQGAS FQPLFFTHD 
      NPFEECFCI CIVLLNKTW KEMRATSED FGKVASVVR 
      EQIVRALGV PPPSSLEQL KVKLQTLTY SDITQLWQL 
      ERTSREEWE SHARPIVEL REQITPDIL NLIKQQRLA 
      FLVDGTRFT KYSARGQRI KDKFWYIRL SPNHKVLHY 
      GDCDEKSTP TTEELPSKL AVADIRALL VGKECPHMK 
      ARKASHQLA FSLALESVD LQSLDCVAP DEETFAYWT 
      DGINALLGQ RMTSKETDR DLDTLLSME IKLRLLDAE 
      GVTIPQDPP PIPPDPVHY HFCYDLK
    CDS sequence
    • >TC002742 RA
      atgcccgca atgaaggac tcgtacatc gtaaaaatc 
      gcggtcgaa atgaccgac caagtgcca caactcatc 
      gaattcaat cagaagcag cccctcgcc ggcataatc 
      caagaactg tgcaacgga tggaatctc agcgacccc 
      gaacagtac gcccttcaa ttcaacgat aacaacaac 
      cgtaactac ataacggag aagaatcgc aacgaaatt 
      aagaacggt tcggtgctc cgtctccaa tactcacct 
      tcgaagaca gctcaggac attctccac aaactgcac 
      aacggaaat gccgaagag aaagcacac gcaatgcac 
      cgtctctca aacctaagc gccgacatg acgttcgct 
      ttggatttc atcaacaag caaggactt actttaata 
      attaagcaa atcgagacg ggtaagttc aagggggct 
      gttctggcc cacacttta ctctccttc gtcgaactg 
      atggaacac gggatcgtg tcgtgggac atcctcaac 
      ggggatttt ataactcgc gttgcgagt tttgttaac 
      tcgccccag gaagccgaa gtcactcaa gccgctctc 
      tcaatcttg gaaaacgtc gttttgaat agtactgaa 
      ggctacggc caggtggaa cgtgagata cctattggt 
      tcgctggtg gggcacctc caggccaca ccggtcgtc 
      caacaaaac gctatagct ttaatcaac gcgttactt 
      ctacgggct gattcagtc aaacgcagg tctttggca 
      gccactctc acaacgaag caggtgcgg agcatgatt 
      cagaatcat attttacaa acgggggct gccttaggg 
      gctgagatg gcccaccag ttgtacgtt ctacagact 
      ttgatgttg gggctgctt gaacagagg atggtcacg 
      aagatggat ccgcaagat caggatggc catgataag 
      atcaaggag ctgcgaagg attgccttt gatagtgaa 
      ggggcgggg ggtgggcga gggccgggt ggtttcaca 
      cgggactac aaaaaattg ggcttcaaa aatgatatt 
      aatccggcg ctggatttc accgagacg ccccctggg 
      ttattggct ttggattgt atgatttat ttcgcacgg 
      aaccacccc gatagttac actaaactt gtcttggag 
      aacagttgt agggctgat gagcacgag tgccccttt 
      gggagggct agtgttgag ttggtccgg attttatgc 
      gagttgctt aaaatcggg gacgctcct tcggagcag 
      ggagcctcc ttccagccg ttgtttttc actcatgat 
      aaccccttt gaggagtgt ttctgcatc tgtattgtt 
      ttgttgaac aagacttgg aaggagatg agggcaact 
      tcggaggat ttcggcaaa gttgcgagt gttgtaagg 
      gagcagatt gtgagggct ttgggagta ccaccgccc 
      tcgtcgttg gaacagctg aaagtgaaa ctccaaact 
      ttgacatac agtgatata acacaattg tggcaattg 
      gagcggact tcgagggag gagtgggaa tcccacgcg 
      agacctatc gttgaatta cgcgaacaa atcactcca 
      gatattctc aacttgata aaacagcaa cgtctcgcg 
      tttctcgtt gatgggaca aggtttaca aaatactca 
      gctcggggg caaaggatc aaggataag ttttggtac 
      atccggctg agccccaat cataaagtc ctccattac 
      ggggattgc gacgagaag agtacgcct accactgag 
      gagcttccg tccaagtta gccgtggct gatattagg 
      gctctcttg gtcgggaaa gagtgtcca catatgaag 
      gcgaggaaa gcctcgcat cagttggcg ttttctctg 
      gctttggaa agtgtcgat ttgcagtca ctggattgt 
      gtggcgccc gatgaagag acgtttgcg tattggacg 
      gatgggatt aatgcgttg ttgggacag aggatgacg 
      agtaaggag acggatcgg gatttggac acgttgctc 
      tcaatggag attaagttg aggttgttg gatgcggaa 
      ggtgtgacg attccgcag gatccaccg ccaattcca 
      ccggatccg gtgcattac cacttttgt tatgatctg 
      aagtag
    mRNA sequence
    • >TC002742 RA
      tgtcattct tgtcaaaat gttgtattt atgaaaaat 
      accctgtaa cgcaaaact cgcgtaata atgttttga 
      atctcaagt gcgcacaaa atgcccgca atgaaggac 
      tcgtacatc gtaaaaatc gcggtcgaa atgaccgac 
      caagtgcca caactcatc gaattcaat cagaagcag 
      cccctcgcc ggcataatc caagaactg tgcaacgga 
      tggaatctc agcgacccc gaacagtac gcccttcaa 
      ttcaacgat aacaacaac cgtaactac ataacggag 
      aagaatcgc aacgaaatt aagaacggt tcggtgctc 
      cgtctccaa tactcacct tcgaagaca gctcaggac 
      attctccac aaactgcac aacggaaat gccgaagag 
      aaagcacac gcaatgcac cgtctctca aacctaagc 
      gccgacatg acgttcgct ttggatttc atcaacaag 
      caaggactt actttaata attaagcaa atcgagacg 
      ggtaagttc aagggggct gttctggcc cacacttta 
      ctctccttc gtcgaactg atggaacac gggatcgtg 
      tcgtgggac atcctcaac ggggatttt ataactcgc 
      gttgcgagt tttgttaac tcgccccag gaagccgaa 
      gtcactcaa gccgctctc tcaatcttg gaaaacgtc 
      gttttgaat agtactgaa ggctacggc caggtggaa 
      cgtgagata cctattggt tcgctggtg gggcacctc 
      caggccaca ccggtcgtc caacaaaac gctatagct 
      ttaatcaac gcgttactt ctacgggct gattcagtc 
      aaacgcagg tctttggca gccactctc acaacgaag 
      caggtgcgg agcatgatt cagaatcat attttacaa 
      acgggggct gccttaggg gctgagatg gcccaccag 
      ttgtacgtt ctacagact ttgatgttg gggctgctt 
      gaacagagg atggtcacg aagatggat ccgcaagat 
      caggatggc catgataag atcaaggag ctgcgaagg 
      attgccttt gatagtgaa ggggcgggg ggtgggcga 
      gggccgggt ggtttcaca cgggactac aaaaaattg 
      ggcttcaaa aatgatatt aatccggcg ctggatttc 
      accgagacg ccccctggg ttattggct ttggattgt 
      atgatttat ttcgcacgg aaccacccc gatagttac 
      actaaactt gtcttggag aacagttgt agggctgat 
      gagcacgag tgccccttt gggagggct agtgttgag 
      ttggtccgg attttatgc gagttgctt aaaatcggg 
      gacgctcct tcggagcag ggagcctcc ttccagccg 
      ttgtttttc actcatgat aaccccttt gaggagtgt 
      ttctgcatc tgtattgtt ttgttgaac aagacttgg 
      aaggagatg agggcaact tcggaggat ttcggcaaa 
      gttgcgagt gttgtaagg gagcagatt gtgagggct 
      ttgggagta ccaccgccc tcgtcgttg gaacagctg 
      aaagtgaaa ctccaaact ttgacatac agtgatata 
      acacaattg tggcaattg gagcggact tcgagggag 
      gagtgggaa tcccacgcg agacctatc gttgaatta 
      cgcgaacaa atcactcca gatattctc aacttgata 
      aaacagcaa cgtctcgcg tttctcgtt gatgggaca 
      aggtttaca aaatactca gctcggggg caaaggatc 
      aaggataag ttttggtac atccggctg agccccaat 
      cataaagtc ctccattac ggggattgc gacgagaag 
      agtacgcct accactgag gagcttccg tccaagtta 
      gccgtggct gatattagg gctctcttg gtcgggaaa 
      gagtgtcca catatgaag gcgaggaaa gcctcgcat 
      cagttggcg ttttctctg gctttggaa agtgtcgat 
      ttgcagtca ctggattgt gtggcgccc gatgaagag 
      acgtttgcg tattggacg gatgggatt aatgcgttg 
      ttgggacag aggatgacg agtaaggag acggatcgg 
      gatttggac acgttgctc tcaatggag attaagttg 
      aggttgttg gatgcggaa ggtgtgacg attccgcag 
      gatccaccg ccaattcca ccggatccg gtgcattac 
      cacttttgt tatgatctg aagtaggga tctgagaat 
      ggcatgaac aagactaat tataccaaa aatcgagat 
      atttattag tattatgaa taattatga tgtaactac 
      tttttagag gtgcatgta agagaaaga agtatatgt 
      attttgcat gaaataaag acttttata ataa
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