Status
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Gene information
General information
TC011026 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6390371..6396161
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011026
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08002

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011026 RA
      MAEAAVEER PPQKFYCHM CNVQFENAS ANFTCPHCA 
      DGFIEELQE SPDSRNPTI DIDDDDDSS DMDFNELLL 
      APTNMEDFR TGRSRAPDG HRTTGRRTL TRLASSNLR 
      QNVPFENLI QDFIVNLGV GLNWGAAGN MQLFLGNPG 
      DYAWGREGL DAIVTQLLN QMDSTGPPP VSKEVIDAL 
      PVINVKSDQ VDAKLQCSV CWEDFQLGE NVRQLPCTH 
      IYHEPCIRP WLELHGTCP ICRQNLVND EQSNSDSNQ 
      DSGGSSTGG QDTLNAIRN ILQPAHNTS SNLENSRPT 
      HSRSDFM
    • >TC011026 RB
      MAEAAVEER PPQKFYCHM CNVQFENAS ANFTCPHCA 
      DGFIEELQE SPDSRNPTI DIDDDDDSS DMDFNELLL 
      APTNMEDFR TGRSRAPDG HRTTGRRYA VNRARIVRP 
      RTLTRLASS NLRQNVPFE NLIQDFIVN LGVGLNWGA 
      AGNMQLFLG NPGDYAWGR EGLDAIVTQ LLNQMDSTG 
      PPPVSKEVI DALPVINVK SDQVDAKLQ CSVCWEDFQ 
      LGENVRQLP CTHIYHEPC IRPWLELHG TCPICRQNL 
      VNDEQSNSD SNQDSGGSS TGGQDTLNA IRNILQPAH 
      NTSSNLENS RPTHSRSDF M
    CDS sequence
    • >TC011026 RA
      atggccgaa gcggctgtg gaagagcgc ccccctcag 
      aaattctac tgtcacatg tgcaacgtc cagttcgaa 
      aacgcctcg gctaacttc acgtgcccc cactgcgcc 
      gacggcttc atcgaggag ctgcaggag agccccgac 
      agccgcaac cccacgata gacatcgac gacgacgac 
      gacagcagc gacatggac ttcaacgag cttttgctc 
      gcgcccacc aacatggaa gactttcga acgggccgc 
      agtcgcgcc cccgacggc catcggacg accggaaga 
      aggaccttg accagacta gcctcctcg aatttgcgg 
      caaaatgtg ccatttgaa aacctcata caggatttt 
      attgtcaat ttaggggtc ggcttgaat tggggcgcc 
      gctggaaat atgcaactg tttctcggg aatccgggg 
      gattatgcc tggggacgc gaaggcctc gatgcgatt 
      gttacgcag ttgttgaat caaatggat agcactggt 
      cctccgccg gtttcgaag gaagtgata gacgccctc 
      cccgtgatc aacgtaaag tcggatcaa gtggacgcg 
      aaactgcag tgttctgtt tgttgggaa gactttcaa 
      ttgggcgaa aacgtccga cagttacct tgcacgcac 
      atttaccat gagccctgt atacgacca tggttggag 
      ttgcatggt acttgtccc atctgccgc cagaaccta 
      gtaaatgat gaacaaagc aacagcgat agtaatcaa 
      gattctggc ggatcgtca accggaggt caagatact 
      ttgaatgcc attaggaat attctccag cctgcccat 
      aatacgtca agtaattta gaaaattcc agacctact 
      cattctaga tcagatttc atgtaa
    • >TC011026 RB
      atggccgaa gcggctgtg gaagagcgc ccccctcag 
      aaattctac tgtcacatg tgcaacgtc cagttcgaa 
      aacgcctcg gctaacttc acgtgcccc cactgcgcc 
      gacggcttc atcgaggag ctgcaggag agccccgac 
      agccgcaac cccacgata gacatcgac gacgacgac 
      gacagcagc gacatggac ttcaacgag cttttgctc 
      gcgcccacc aacatggaa gactttcga acgggccgc 
      agtcgcgcc cccgacggc catcggacg accggaaga 
      aggtatgca gttaatcgc gccaggata gtacgaccc 
      aggaccttg accagacta gcctcctcg aatttgcgg 
      caaaatgtg ccatttgaa aacctcata caggatttt 
      attgtcaat ttaggggtc ggcttgaat tggggcgcc 
      gctggaaat atgcaactg tttctcggg aatccgggg 
      gattatgcc tggggacgc gaaggcctc gatgcgatt 
      gttacgcag ttgttgaat caaatggat agcactggt 
      cctccgccg gtttcgaag gaagtgata gacgccctc 
      cccgtgatc aacgtaaag tcggatcaa gtggacgcg 
      aaactgcag tgttctgtt tgttgggaa gactttcaa 
      ttgggcgaa aacgtccga cagttacct tgcacgcac 
      atttaccat gagccctgt atacgacca tggttggag 
      ttgcatggt acttgtccc atctgccgc cagaaccta 
      gtaaatgat gaacaaagc aacagcgat agtaatcaa 
      gattctggc ggatcgtca accggaggt caagatact 
      ttgaatgcc attaggaat attctccag cctgcccat 
      aatacgtca agtaattta gaaaattcc agacctact 
      cattctaga tcagatttc atgtaa
    mRNA sequence
    • >TC011026 RA
      actgaacat tgacttcaa catttaata tggtggtca 
      aaaaagggc agataattg ggaatttgt gaaaaagta 
      gttgtgtag cgcagcccc cgagcccca acccccgaa 
      aatggccga agcggctgt ggaagagcg cccccctca 
      gaaattcta ctgtcacat gtgcaacgt ccagttcga 
      aaacgcctc ggctaactt cacgtgccc ccactgcgc 
      cgacggctt catcgagga gctgcagga gagccccga 
      cagccgcaa ccccacgat agacatcga cgacgacga 
      cgacagcag cgacatgga cttcaacga gcttttgct 
      cgcgcccac caacatgga agactttcg aacgggccg 
      cagtcgcgc ccccgacgg ccatcggac gaccggaag 
      aaggacctt gaccagact agcctcctc gaatttgcg 
      gcaaaatgt gccatttga aaacctcat acaggattt 
      tattgtcaa tttaggggt cggcttgaa ttggggcgc 
      cgctggaaa tatgcaact gtttctcgg gaatccggg 
      ggattatgc ctggggacg cgaaggcct cgatgcgat 
      tgttacgca gttgttgaa tcaaatgga tagcactgg 
      tcctccgcc ggtttcgaa ggaagtgat agacgccct 
      ccccgtgat caacgtaaa gtcggatca agtggacgc 
      gaaactgca gtgttctgt ttgttggga agactttca 
      attgggcga aaacgtccg acagttacc ttgcacgca 
      catttacca tgagccctg tatacgacc atggttgga 
      gttgcatgg tacttgtcc catctgccg ccagaacct 
      agtaaatga tgaacaaag caacagcga tagtaatca 
      agattctgg cggatcgtc aaccggagg tcaagatac 
      tttgaatgc cattaggaa tattctcca gcctgccca 
      taatacgtc aagtaattt agaaaattc cagacctac 
      tcattctag atcagattt catgtaatc actacaagt 
      ttcaattag aaagaaata tcaacgctt gctataaat 
      tcaaattcg ttcgttttc cgaggatgc tttatacag 
      aatgagcaa acgagtttt taattgtta atataatga 
      gaaataaag ttgtacaga ctggataaa gcgtatttt 
      taagtattc tgtaacaag tggcacttt aaggtttgt 
      ccagcaagt tacgttatg ttatgatta tgtatttat 
      gattacact gttgtgata ataaaaaat atccttacg 
      a
    • >TC011026 RB
      actgaacat tgacttcaa catttaata tggtggtca 
      aaaaagggc agataattg ggaatttgt gaaaaagta 
      gttgtgtag cgcagcccc cgagcccca acccccgaa 
      aatggccga agcggctgt ggaagagcg cccccctca 
      gaaattcta ctgtcacat gtgcaacgt ccagttcga 
      aaacgcctc ggctaactt cacgtgccc ccactgcgc 
      cgacggctt catcgagga gctgcagga gagccccga 
      cagccgcaa ccccacgat agacatcga cgacgacga 
      cgacagcag cgacatgga cttcaacga gcttttgct 
      cgcgcccac caacatgga agactttcg aacgggccg 
      cagtcgcgc ccccgacgg ccatcggac gaccggaag 
      aaggtatgc agttaatcg cgccaggat agtacgacc 
      caggacctt gaccagact agcctcctc gaatttgcg 
      gcaaaatgt gccatttga aaacctcat acaggattt 
      tattgtcaa tttaggggt cggcttgaa ttggggcgc 
      cgctggaaa tatgcaact gtttctcgg gaatccggg 
      ggattatgc ctggggacg cgaaggcct cgatgcgat 
      tgttacgca gttgttgaa tcaaatgga tagcactgg 
      tcctccgcc ggtttcgaa ggaagtgat agacgccct 
      ccccgtgat caacgtaaa gtcggatca agtggacgc 
      gaaactgca gtgttctgt ttgttggga agactttca 
      attgggcga aaacgtccg acagttacc ttgcacgca 
      catttacca tgagccctg tatacgacc atggttgga 
      gttgcatgg tacttgtcc catctgccg ccagaacct 
      agtaaatga tgaacaaag caacagcga tagtaatca 
      agattctgg cggatcgtc aaccggagg tcaagatac 
      tttgaatgc cattaggaa tattctcca gcctgccca 
      taatacgtc aagtaattt agaaaattc cagacctac 
      tcattctag atcagattt catgtaatc actacaagt 
      ttcaattag aaagaaata tcaacgctt gctataaat 
      tcaaattcg ttcgttttc cgaggatgc tttatacag 
      aatgagcaa acgagtttt taattgtta atataatga 
      gaaataaag ttgtacaga ctggataaa gcgtatttt 
      taagtattc tgtaacaag tggcacttt aaggtttgt 
      ccagcaagt tacgttatg ttatgatta tgtatttat 
      gattacact gttgtgata ataaaaaat atccttacg 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY