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Gene information
General information
TC011027 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6388711..6390321
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011027
    iBeetle screen iB_08001

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011027 RA
      MRECISVHI GQAGVQIGN TTWELYCLE HGIQPDGSL 
      KDQKDIVSD NCYGTFFYE VENGNMVPR SLMVDLEPT 
      VVDEVRVGK YKQLYHPEQ LITGKEDAA NNYARGHYT 
      IGKEMIHVV MDKLSKMAE QCSGLQGFL VFHSFGGGT 
      GSGFTSLLM ENLSDEFGK KSKLEFVIY PAPQVCTAV 
      VEPYNSILT THTTLGHTD CAFMVDNEA IYDICRKKL 
      GIERPNYIN LNRLISQVV SSITASLRF DGALNVDLT 
      EYQTNLVPY PRIHFPLAS YAPVVSSDK AFHEGMSVS 
      EITSELFES SNQMVKCDP RHGKYMACC LLYRGDVVP 
      KDVNASIAQ MKTKANIRF VNWCPTGFK VGINYQPPT 
      VVPGGDLAR VQRAACMLS NTTAIESAW NKLNKKFDL 
      MFAKRAFVH WYIHEGMEE GEFNEARED LAMLEKDYE 
      EVSIDDIE
    CDS sequence
    • >TC011027 RA
      atgagggag tgtatttcg gtgcacatt ggccaagct 
      ggggtccag atcggcaac accacctgg gagctgtac 
      tgtctcgag cacggaatt cagcccgac ggcagtctc 
      aaggaccag aaggacatc gtctccgac aactgctac 
      ggcacgttt ttctacgag gtggagaac gggaacatg 
      gtcccgcgc agcctcatg gtggacctg gagcccacc 
      gtcgtggat gaggtccgc gtgggtaaa tacaagcag 
      ttgtaccac ccggagcag ctcataacc ggcaaagag 
      gacgccgcc aacaactac gcccgcggc cactacacc 
      atcggcaag gagatgatc cacgtggtg atggacaag 
      ctctcgaag atggccgag cagtgctcg ggcctgcag 
      ggcttcctc gtgttccac tcgttcggc ggcggcacc 
      ggctccggc ttcacctcc ctcctcatg gagaacctc 
      tccgacgag ttcggcaag aagagcaaa ctggagttc 
      gtcatctac ccggcgccc caggtgtgc acggccgtg 
      gtggagccc tacaactcc atcctcacc acgcacacc 
      accctgggc cacaccgac tgcgccttt atggtggac 
      aacgaggcc atctacgac atctgccgc aagaagctg 
      ggcatcgag cgccccaac tacatcaac ttgaaccgg 
      ctcatcagc caggtggtg tcgtccatc acggccagt 
      ctgcgcttc gacggcgcg ctcaacgtc gacctcacc 
      gagtaccag accaacctg gtgccctac ccaaggata 
      cacttcccg ctggcgtcg tacgcgccc gtggtgtcc 
      tccgacaag gccttccac gagggcatg tccgtgtcg 
      gagatcacg tccgagttg ttcgagtcg tccaaccag 
      atggtcaag tgcgacccg agacatggc aagtacatg 
      gcgtgctgt ctgctctac aggggggac gtggtgccg 
      aaggacgtg aacgcgtcc atcgcgcag atgaagacc 
      aaggcgaat attaggttc gtgaactgg tgtcccacc 
      gggttcaag gtcgggatt aactaccag ccgccgacg 
      gtggtgccg gggggcgac ttggccagg gtgcagagg 
      gccgcctgc atgctgtcg aacacgacg gccatcgag 
      tcggcgtgg aataagctg aacaagaag tttgatctg 
      atgtttgcg aagagagcc ttcgtgcat tggtacata 
      catgagggg atggaggaa ggggagttt aatgaggcg 
      agggaggac ttggccatg ctggagaag gactatgag 
      gaggtttcc atcgatgat attgaatag
    mRNA sequence
    • >TC011027 RA
      ttttcacaa aattctaat gagttttcg catccctcc 
      aaatttatg tagttaaaa tttgtggtg tgtgttaaa 
      ttggaaatt tcgccagaa tgagggagt gtatttcgg 
      tgcacattg gccaagctg gggtccaga tcggcaaca 
      ccacctggg agctgtact gtctcgagc acggaattc 
      agcccgacg gcagtctca aggaccaga aggacatcg 
      tctccgaca actgctacg gcacgtttt tctacgagg 
      tggagaacg ggaacatgg tcccgcgca gcctcatgg 
      tggacctgg agcccaccg tcgtggatg aggtccgcg 
      tgggtaaat acaagcagt tgtaccacc cggagcagc 
      tcataaccg gcaaagagg acgccgcca acaactacg 
      cccgcggcc actacacca tcggcaagg agatgatcc 
      acgtggtga tggacaagc tctcgaaga tggccgagc 
      agtgctcgg gcctgcagg gcttcctcg tgttccact 
      cgttcggcg gcggcaccg gctccggct tcacctccc 
      tcctcatgg agaacctct ccgacgagt tcggcaaga 
      agagcaaac tggagttcg tcatctacc cggcgcccc 
      aggtgtgca cggccgtgg tggagccct acaactcca 
      tcctcacca cgcacacca ccctgggcc acaccgact 
      gcgccttta tggtggaca acgaggcca tctacgaca 
      tctgccgca agaagctgg gcatcgagc gccccaact 
      acatcaact tgaaccggc tcatcagcc aggtggtgt 
      cgtccatca cggccagtc tgcgcttcg acggcgcgc 
      tcaacgtcg acctcaccg agtaccaga ccaacctgg 
      tgccctacc caaggatac acttcccgc tggcgtcgt 
      acgcgcccg tggtgtcct ccgacaagg ccttccacg 
      agggcatgt ccgtgtcgg agatcacgt ccgagttgt 
      tcgagtcgt ccaaccaga tggtcaagt gcgacccga 
      gacatggca agtacatgg cgtgctgtc tgctctaca 
      ggggggacg tggtgccga aggacgtga acgcgtcca 
      tcgcgcaga tgaagacca aggcgaata ttaggttcg 
      tgaactggt gtcccaccg ggttcaagg tcgggatta 
      actaccagc cgccgacgg tggtgccgg ggggcgact 
      tggccaggg tgcagaggg ccgcctgca tgctgtcga 
      acacgacgg ccatcgagt cggcgtgga ataagctga 
      acaagaagt ttgatctga tgtttgcga agagagcct 
      tcgtgcatt ggtacatac atgagggga tggaggaag 
      gggagttta atgaggcga gggaggact tggccatgc 
      tggagaagg actatgagg aggtttcca tcgatgata 
      ttgaatagt cactcacgc atttacact tcattttga 
      ttatacaaa ttggcaaag ccagtggtg actgcgctt 
      atgagttat attttttga acatacaat aaatattta 
      ataaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY