Status
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Gene information
General information
TC002763 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12165665..12171244
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002763
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08000

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002763 RA
      MGKPEFKSP RLRSAPIRS RLLGKKGPT VRSSANIRV 
VVRVRPPNH KEQGDNSRD VVKIVDDQV LIFDPKCQS 
QAFFYHGVE QKGRDLLRK ANKDMQFMF DRVFGFEST 
NCEVFENTT KGLIQSLMD GYNCSVFAY GATGAGKTH 
TMIGQTDNP GITYLTMAE LFKAKQDLQ EERKFELGI 
SYIEVYNEL VQDLLNPGA PLQLRDDGR YGVMVAGIK 
VHKIDNPDE LFTLLAKGN GNRTQHPTD ANAESSRSH 
AVFQVYIQM EIKATREVR AAKLSMIDL AGSERGSAT 
GYGGARFAE GANINKSLL ALGNCINSL ADGQKYIPY 
RDSKLTRLL KDSLGGNCQ TVMVANVSP SSLCYDDTY 
NTLKYATRA KKIKSDVKK NVVNVELHA GEYVKIVED 
LKKELERVK AQLREKVTE NKVDGKVAL EFGKLIESR 
KKLLNRMYH VECSETTTV LRRQLKEDA DSRLSGLLN 
ETPEKEEAH RRLGNMVGR FHRQEDSLR AELDEITKS 
KIELENKTI DLVLQHPEL EKKSELCEE KIGKMELQY 
QVNLLQKRC SILEEELRS RANLLEMSC KMLQSCYMQ 
LRGHGFATD TLCQEYQNL VNHVQGRRN ISWDSPLDS 
GVSSTSSGT SIDGEGDAR APRKRANDE NVNGMNSTF 
VMEAPKPKV KVCAEELMA KVYKSPNTK PKKEGMKDR 
RAVAAKRPL TPVLRKKQL TKSEALKKR PAFRT
    CDS sequence
    • >TC002763 RA
      atgggcaag ccggagttt aagtcgcct cggctgagg 
agcgcccca atcaggtcg cgtcttttg ggcaagaag 
ggccccaca gtgcggtct tcggccaac atccgggtc 
gtggtgcgc gtccggccg ccgaaccac aaagaacag 
ggcgataac agcagagat gttgtgaag attgtcgac 
gatcaggtg ttgattttc gacccgaaa tgccagtcg 
caggcgttt ttctaccac ggggtcgag cagaagggg 
cgcgatttg ttgcgcaaa gccaataaa gacatgcag 
tttatgttc gatagggtt ttcgggttc gagtcgacc 
aattgcgag gtgtttgaa aacacgaca aaggggctc 
atccagagc ctgatggat gggtacaac tgttcggtg 
tttgcctat ggggccaca ggggctggg aaaacgcac 
accatgatt ggccagacg gacaatcct ggcatcacc 
tacctaacg atggctgag ctgttcaaa gccaagcag 
gatttgcag gaggagcgc aagttcgag ctgggcatt 
tcgtacatt gaggtttac aacgaactg gtgcaggat 
ctgttgaac ccgggggcc cccttgcag ttgcgggat 
gacgggcgc tatggggtc atggtggct gggattaaa 
gtccacaaa atcgataat ccggacgaa cttttcaca 
ctgttagca aaagggaac ggtaatcgg acccagcac 
cccactgac gccaacgcg gagagctcc aggtcgcac 
gcagtcttt caagtttac atccagatg gagattaag 
gccacgcgc gaagtcagg gcggccaaa ctcagcatg 
attgattta gctgggagt gagcggggc tccgccacc 
ggatatggg ggcgcgagg tttgccgaa ggcgccaat 
ataaacaag tcgctgctt gcgcttggc aactgtatt 
aatagtctt gccgatggc caaaaatat atcccgtat 
agagactcg aaattaacc agattactc aaagacagt 
ctagggggc aattgccag actgttatg gtggcgaat 
gtgtctcca tcgtcgctc tgttacgac gatacttac 
aatactttg aaatatgcc acaagggcg aagaaaatc 
aagtccgat gttaagaaa aatgtggtc aatgtcgaa 
ttgcatgcg ggagaatat gtgaaaatt gttgaagat 
ttgaaaaag gagttggag agggttaag gcacagctt 
agggagaaa gtcactgaa aataaggtt gacgggaag 
gtggctctt gaatttggg aaactgatt gaaagtaga 
aagaagttg ctgaatagg atgtaccat gtggagtgt 
tcagaaacg accaccgtt ttgaggaga cagttgaaa 
gaagacgct gattcgcga ctgagcggt ttattgaat 
gaaacaccc gaaaaagag gaagctcac cgcagactc 
ggcaatatg gttggtcgc ttccaccgc caagaagac 
agccttcgc gccgaattg gacgaaata accaaatcg 
aaaatcgag ttggaaaac aaaaccatc gacttggtc 
ctccaacac cccgaactc gagaaaaag tccgaactg 
tgcgaggag aaaatcggc aaaatggaa ttgcaatac 
caagtcaac ctgttgcaa aaacggtgc agcatcctg 
gaagaagag ctgcgttcg cgggccaac ctcctggag 
atgagctgc aagatgctg cagagttgc tacatgcag 
ttgcgaggg cacggcttt gccaccgac accctctgt 
caggagtac cagaatttg gtgaaccac gtccaaggc 
aggaggaac atcagctgg gacagcccc ctcgactcg 
ggggtgagc tccacgtcc agcggcacc tccatcgac 
ggggagggc gacgccaga gcccccagg aaaagggcc 
aacgacgag aatgtcaac gggatgaac agcaccttt 
gtcatggaa gcccccaag cccaaagtg aaagtgtgt 
gccgaagaa ctgatggct aaagtgtac aaatcgcca 
aatactaaa ccgaaaaag gagggcatg aaggacagg 
agggccgta gcggccaaa cgcccctta actccagtg 
ctgcgcaag aaacaactg actaaatcc gaagcgtta 
aaaaaacgt cctgcgttc aggacttga
    mRNA sequence
    • >TC002763 RA
      taacggtcg acttgatgg ttgttttca attgtgtcg 
ttgtcctgt cacaaaact cgtaaattc tcgccaaag 
tgtttgaaa cgtcaaaaa ttgatgaaa gtttacgat 
tttctccac tctctttta tacagaatt gtgcagatg 
tgatatttt cggtgtgta caaattatt taacgagtg 
aagtgtttt atttgcgta aaatgggca agccggagt 
ttaagtcgc ctcggctga ggagcgccc caatcaggt 
cgcgtcttt tgggcaaga agggcccca cagtgcggt 
cttcggcca acatccggg tcgtggtgc gcgtccggc 
cgccgaacc acaaagaac agggcgata acagcagag 
atgttgtga agattgtcg acgatcagg tgttgattt 
tcgacccga aatgccagt cgcaggcgt ttttctacc 
acggggtcg agcagaagg ggcgcgatt tgttgcgca 
aagccaata aagacatgc agtttatgt tcgataggg 
ttttcgggt tcgagtcga ccaattgcg aggtgtttg 
aaaacacga caaaggggc tcatccaga gcctgatgg 
atgggtaca actgttcgg tgtttgcct atggggcca 
caggggctg ggaaaacgc acaccatga ttggccaga 
cggacaatc ctggcatca cctacctaa cgatggctg 
agctgttca aagccaagc aggatttgc aggaggagc 
gcaagttcg agctgggca tttcgtaca ttgaggttt 
acaacgaac tggtgcagg atctgttga acccggggg 
cccccttgc agttgcggg atgacgggc gctatgggg 
tcatggtgg ctgggatta aagtccaca aaatcgata 
atccggacg aacttttca cactgttag caaaaggga 
acggtaatc ggacccagc accccactg acgccaacg 
cggagagct ccaggtcgc acgcagtct ttcaagttt 
acatccaga tggagatta aggccacgc gcgaagtca 
gggcggcca aactcagca tgattgatt tagctggga 
gtgagcggg gctccgcca ccggatatg ggggcgcga 
ggtttgccg aaggcgcca atataaaca agtcgctgc 
ttgcgcttg gcaactgta ttaatagtc ttgccgatg 
gccaaaaat atatcccgt atagagact cgaaattaa 
ccagattac tcaaagaca gtctagggg gcaattgcc 
agactgtta tggtggcga atgtgtctc catcgtcgc 
tctgttacg acgatactt acaatactt tgaaatatg 
ccacaaggg cgaagaaaa tcaagtccg atgttaaga 
aaaatgtgg tcaatgtcg aattgcatg cgggagaat 
atgtgaaaa ttgttgaag atttgaaaa aggagttgg 
agagggtta aggcacagc ttagggaga aagtcactg 
aaaataagg ttgacggga aggtggctc ttgaatttg 
ggaaactga ttgaaagta gaaagaagt tgctgaata 
ggatgtacc atgtggagt gttcagaaa cgaccaccg 
ttttgagga gacagttga aagaagacg ctgattcgc 
gactgagcg gtttattga atgaaacac ccgaaaaag 
aggaagctc accgcagac tcggcaata tggttggtc 
gcttccacc gccaagaag acagccttc gcgccgaat 
tggacgaaa taaccaaat cgaaaatcg agttggaaa 
acaaaacca tcgacttgg tcctccaac accccgaac 
tcgagaaaa agtccgaac tgtgcgagg agaaaatcg 
gcaaaatgg aattgcaat accaagtca acctgttgc 
aaaaacggt gcagcatcc tggaagaag agctgcgtt 
cgcgggcca acctcctgg agatgagct gcaagatgc 
tgcagagtt gctacatgc agttgcgag ggcacggct 
ttgccaccg acaccctct gtcaggagt accagaatt 
tggtgaacc acgtccaag gcaggagga acatcagct 
gggacagcc ccctcgact cgggggtga gctccacgt 
ccagcggca cctccatcg acggggagg gcgacgcca 
gagccccca ggaaaaggg ccaacgacg agaatgtca 
acgggatga acagcacct ttgtcatgg aagccccca 
agcccaaag tgaaagtgt gtgccgaag aactgatgg 
ctaaagtgt acaaatcgc caaatacta aaccgaaaa 
aggagggca tgaaggaca ggagggccg tagcggcca 
aacgcccct taactccag tgctgcgca agaaacaac 
tgactaaat ccgaagcgt taaaaaaac gtcctgcgt 
tcaggactt gaggctttt attcatgtt gaagttttt 
atattttaa tttttatac taaatattt tattttgtt 
agaattaaa ccataattt tgtacatgg atgcgataa 
aaaattaaa attagaata atcgattgt tgaggtgcg 
tgaaatagt ttatagctc gtgctacag tattttcca 
ctgtgttgt cctcaaact gttcgcggg caaacacta 
ccggcgtga gtctcgcac gtgcaatca caattaaca 
agatcacaa ttattatcg aaaagatca ctgcactag 
ccaggaaca ccagcggca tgaggaagt agtgaggca 
tcgcaggag gccccgaat gcgtctcga tgatctcac 
agtggtggt gatgcggat gtcctatgg ccattattt 
attcgactt atttaaaca ctagttaga tgcattgac 
tgactcttg tattctaat gtaaaataa acaatctac 
ccattt
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