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Gene information
General information
TC002774 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11719929..11752343
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002774
    iBeetle screen iB_07999

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002774 RA
      MKLSVLLTT QFRSPRITE HPSDIIVAK 
      NEPVTLNCK AEGRPEPTI EWFKDGEPV 
      KTSPTDNKS HRVLLPAGS LFFLRTMNS 
      KKEQDAGVY WCVARNTAG SAVSRNATL 
      QVAGIDLLE IKLL
    CDS sequence
    • >TC002774 RA
      atgaagctg tcagttcta ctcacaacc cagtttcgc 
      tcgccgcgg atcaccgag cacccctcc gacataatc 
      gtggccaag aacgaacca gtgaccctc aactgtaag 
      gccgagggc cgtcccgag cccaccatc gagtggttc 
      aaagacgga gaacccgtc aaaacctca cccaccgac 
      aacaaatcg caccgagtc ctgctgccg gcaggatcc 
      ctcttcttc ctgcgaacc atgaacagc aagaaggag 
      caggacgcg ggtgtctac tggtgcgtg gctcgcaac 
      acggccggc tctgcggtc agcaggaat gccacgctg 
      caagtagcc ggaattgat cttttggaa attaaatta 
      ctatga
    mRNA sequence
    • >TC002774 RA
      actcatgcg caagctcac tcgtgagtc aaaattttc 
      actcgcata taatgatgc atttctatc acttatatt 
      atattatac tattatctt acaggtgca gtttttaag 
      gtacattat atcattgtt ttcggtgag agaaatcaa 
      atttttctc acaagaaag atgaagctg tcagttcta 
      ctcacaacc cagtttcgc tcgccgcgg atcaccgag 
      cacccctcc gacataatc gtggccaag aacgaacca 
      gtgaccctc aactgtaag gccgagggc cgtcccgag 
      cccaccatc gagtggttc aaagacgga gaacccgtc 
      aaaacctca cccaccgac aacaaatcg caccgagtc 
      ctgctgccg gcaggatcc ctcttcttc ctgcgaacc 
      atgaacagc aagaaggag caggacgcg ggtgtctac 
      tggtgcgtg gctcgcaac acggccggc tctgcggtc 
      agcaggaat gccacgctg caagtagcc ggaattgat 
      cttttggaa attaaatta ctatgagga agggaggtt 
      gagataagg cgtgtggtt caagtgtgc aaagtttgc 
      gcattttgc agcgccata acaatctag aaaacgtcc 
      agcaaaggt cactagcac gaacttgtc tcatgcaat 
      ctccccgaa acaatagcg tatgaccac aattatgac 
      atacagccc atttagtca gcgataatg gaattttca 
      gctaataac gtaattctg tattattgc aatcgaact 
      ggctgtccc tagcagaca acttgatgg cagatttca 
      gtaattggc gtgactgtt ggaccagat atgtctaat 
      agcgctaat accggtttt agtatcggg ggttggaaa 
      attgtaagt atccccaat gccacccca cggacatgt 
      aagctttat ttgcttgct cctagcact aatattatc 
      cctagggat gaagggcgt tttatctaa tataccagt 
      cctaatgcc aaagccata aataatttc cgccgatat 
      gcaagcgac ggtgacatg aatggtttc actgatata 
      attgcacca accctacaa ccagataaa acccaaatt 
      cactcctgc gtgggttcg cttctccgt aaagtcatt 
      tttgatttt taatgtctc tttgccttc acacggccg 
      gcaataaaa accgaatgt ttattattc aaagcggca 
      ataaattga gaaagtaaa gaaaatttt ttaggcgtg 
      ttgttggtt cagagaaga gactggtag ccgcacaca 
      aatattgac tgtcgagct tctgaatac gtcgtatgt 
      ttgcgatta tgctaatga tgcccattt gacaaattg 
      actcgagaa tacacacga aagagtatg tttgtgagc 
      acttcaaga gctttcaac atcgttccg ctgcagcaa 
      aacttgata aatccatta aaatatcaa ttacgcccg 
      cttttagtt tctcttaat tgtgtttgc tctcttccg 
      aggttccga agcaacaac ccgaacact tgcgcaacc 
      agaaattta caaaaacac agagaaaat agtaagcac 
      gaaagtgct aacaaaatg cggaaatga acccaaaaa 
      atataaaaa gaaatttcc caggaaaat ttcactctc 
      acaacttct attactatt tctttctcc atttggaaa 
      cacaccgtt tcagaataa aactatgaa agattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY