Status
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Gene information
General information
TC000140 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10655226..10664375
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000140
    iBeetle screen iB_07997

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber potentially not present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber shape irregular - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000140 RA
      MKKTKVLNA SKHNWKISD FFTKCPNPS RTVSCENKR 
      KNSDFPIKT EPQSPEKCN AESPNSKRR KFDENSDPL 
      DSHNITIKI EPLSQVCDS GGTSFNVVT PEKKFKPSS 
      SMKTPTPKK IEILASSNQ RRTPQSGRK TTPKKLFEK 
      SPRKLEEFN VQILENFVK VSPSTSNRK IVIKSTPTK 
      NHAQSHIDE FLSPTISKS PAQSRNETT PSKARTRLY 
      FVDDKKPSP MDDLDQFFN DSFEDLVIN CNTLDLGEP 
      QHCQIIQVS HNGGKIVLN LKSTKGGET GFCYIEQFW 
      ASGNRFKTG DSVYIKAEK NSNGDWVVN NDTGWLVYQ 
      PDILISCTS VVGSLFCKR KAVLSDRFR GFEPTNRVM 
      LIGTLAHTL LQQSLRKKL TKRKQIEAI LKNLLASKD 
      TIKRLYEAN LTVADLESD LEVFVPKLV AFISEHITT 
      GQICDIEDI EENLWCPQL GLKGKIDVT VRSNTGVMP 
      LEVKTGRAT VSLEHRGQV VLYLMMMNK FGLDVSSGL 
      LLYLKEGVL KEIKATEQE KRDVLILRN ELAYFLASD 
      VGELPEPLN NIRVCQKCA LSTVCSAYL KFEDHDLTE 
      NKAMQEVQK KSIGHLSQA HLDYFLKWS NLLSMEAQH 
      SKTTKSLQQ IYTLPPAKR EALGRSIVN MRVTFVENE 
      TNGLFKHTM EKENPSLAN LTAYDIIEN NYIVVSTDS 
      RPAVASGFV TEVSPTQIS ITLDRDLNV KYRNQLFHL 
      DTYDSNITF TFNMTSLVL LIENSERAA QLRSVIIDK 
      APPTFAPKL PSVVAKKGA PILRRLNHV QQKAVLKAL 
      CANQFLLIK GMPGTGKSA TIVALIQLL IELNKSVLI 
      TSHTHSAID NICIRLQKL NVDFIRLGS ESKIDSKLK 
      PNLETTLTE KCTTPEEMN EIYNKYKVI AVTCLGSGH 
      PLLTKRTVD VCIVDESTQ VLQTSVIKP LFAAKSFIL 
      IGDPHQLAP LVRQRDALE AGMSESLFE RLENSEGTI 
      TLNLNYRMN KTITELANA LTYQGQLQT ASEEVANRV 
      LKLPKIEIV KKEYKEHQW LLNCLDVSL EGAVQFIDT 
      GPVSELTNH ADWITKTVK DGCDLQNCI NVYEIAIVV 
      TLVKALILG GVASSDIGV IATYRAQVS QITDLLEKY 
      QVDVSTVDQ FQGRDKSVI IYSCTKSSA QVATNTLKY 
      ELLEDKRRL TVAVTRAKR KLLIVGDSS TLVRYKTFE 
      TILTHLEKH IVKFDSEWS ILNSL
    CDS sequence
    • >TC000140 RA
      atgaaaaaa actaaagtt cttaacgcc tctaaacac 
      aattggaaa atttccgat ttttttaca aaatgtccc 
      aacccttca cggactgta agctgtgaa aacaaaagg 
      aaaaattcc gattttccc ataaagact gagccccaa 
      tcacccgaa aaatgtaac gcggagtca ccaaatagc 
      aaacgaaga aaattcgat gaaaatagc gatccttta 
      gattctcac aatataaca atcaagatt gaaccctta 
      tctcaagtt tgtgacagt ggtggtact agttttaac 
      gtcgtaaca cctgaaaaa aaattcaaa ccttccagt 
      tccatgaag acacccacc cctaaaaaa atcgagatt 
      ttggccagt tctaatcaa aggaggacg ccccaaagt 
      gggcggaaa acaacgcca aagaaatta tttgaaaaa 
      tcgccgagg aaattggag gaatttaac gtccaaatt 
      ttggaaaat ttcgtcaaa gtgtctccg agcacttcc 
      aacagaaaa atcgtaatt aaaagcacg ccgaccaaa 
      aaccatgcg cagagtcac atcgatgaa tttttatct 
      ccaactatt tcaaagtct cctgcccaa agtcgcaat 
      gaaaccact ccgtccaaa gccagaact cggttatat 
      ttcgtggat gataaaaaa ccgagtcca atggatgat 
      ttagatcaa ttttttaac gattcgttt gaggattta 
      gttattaat tgcaataca ttggatttg ggggaacca 
      cagcattgt cagatcatc caagtgtca cacaatgga 
      ggcaaaatc gtcttgaat ttaaaatcg accaagggc 
      ggggagact ggtttttgt tacattgaa caattttgg 
      gcttccgga aaccgattc aaaaccggc gattctgtc 
      tacatcaag gcggaaaaa aactcaaac ggtgattgg 
      gtcgttaac aacgacacg ggatggcta gtttaccaa 
      ccagatata ttaatttcg tgcacttca gtcgtcgga 
      agtctattt tgcaaacgc aaagctgtc ttatccgac 
      cgtttccgt ggcttcgaa ccaacaaac cgcgtcatg 
      ctaatcggc actttagca cacaccctc ctccaacaa 
      tctttgaga aaaaaatta acaaaaagg aagcaaatt 
      gaggcgatt ttgaaaaat ttgctcgca tccaaagac 
      acgataaaa agattgtac gaagccaac ctaaccgtt 
      gccgacctt gaatcagac ttggaggtg tttgtgccc 
      aaattggtc gcttttatc agcgaacac ataacaacg 
      gggcaaatc tgcgacatt gaggatatt gaggagaat 
      ttgtggtgc cctcagttg gggctcaag ggcaaaatc 
      gatgttaca gtgcggtca aataccggc gttatgccc 
      ctcgaggtc aagacaggt cgagcgact gtctcactt 
      gagcacaga ggacaagtc gttttatac ttaatgatg 
      atgaataag ttcgggctt gatgtctct tctggtctg 
      ttgctctac ttgaaagaa ggggttttg aaggaaatt 
      aaggccaca gagcaagaa aaacgtgat gttttgatt 
      ttacggaac gaattggcg tattttttg gccagtgac 
      gtaggggaa ttaccggag ccgttgaat aatattcgg 
      gtttgtcag aagtgtgct ttgagtacc gtttgcagc 
      gcctatttg aagtttgaa gatcacgat ttaacagaa 
      aataaagcg atgcaagaa gtacaaaag aaatcaatt 
      ggtcatttg agtcaagca cacttagat tattttctc 
      aaatggagt aatttgttg agcatggaa gcacaacat 
      agtaaaaca acaaagtcg ctacaacaa atttacact 
      ttaccaccg gctaaaaga gaagcttta ggtcgatcg 
      attgtcaac atgagagtg acttttgtc gaaaatgaa 
      accaacggt ttatttaaa cacacaatg gagaaggaa 
      aatcccagt ttggccaac cttacggct tacgatatc 
      attgaaaat aattacata gtcgtcagc actgattcc 
      cggcctgct gttgcgtca ggattcgta acagaagtc 
      tccccaact caaatcagt attacactt gatagagat 
      ctcaatgta aaatatcgc aatcaattg tttcatctc 
      gacacctac gattcaaat atcactttt acttttaat 
      atgacaagt ttagtgttg cttattgag aattcggaa 
      agggctgct cagttaagg agtgttata attgataaa 
      gctccgccc acttttgca ccaaaactg ccgtcagtt 
      gttgcgaaa aagggggcc ccgatttta cgacgtcta 
      aatcatgtg caacagaag gctgtgctt aaagcattg 
      tgtgcaaat cagtttttg ctgatcaag gggatgcca 
      ggcactggt aaaagcgcc accattgtt gccctaatc 
      caactgcta atcgaactg aataaaagt gtcctcata 
      actagtcat actcattca gccattgac aacatttgt 
      atccgtttg cagaaacta aacgttgac ttcataaga 
      ctcggatct gagagtaaa atcgattcg aaattgaaa 
      ccaaatttg gaaacgact ctcactgaa aaatgtaca 
      acacccgaa gagatgaac gaaatttac aacaaatac 
      aaagtgatt gcggttacg tgtctcggc tccggccac 
      cctctcctc accaaacgc actgtagac gtatgtata 
      gtcgatgaa agcactcaa gtcctccaa acgtcagtg 
      attaagccg ttatttgct gccaaatct tttattctg 
      attggtgac ccccaccaa cttgcacct ctagtacgc 
      caacgtgat gccttagag gcaggaatg tcggaaagt 
      cttttcgaa agactggaa aattctgaa gggacaatt 
      accctaaac ctcaactat cgcatgaat aaaacaatc 
      acggagttg gccaatgct ctcacgtat caaggccaa 
      ctacagacg gccagtgaa gaagtggca aaccgtgtc 
      ttgaaactg cccaaaatt gagatcgtt aaaaaagag 
      tacaaagag caccagtgg ctgctcaac tgcctcgat 
      gtgtcgtta gagggcgct gtacaattt atcgacacc 
      ggccctgta tccgaatta accaatcac gctgattgg 
      attaccaaa actgtgaaa gacggttgt gatctgcag 
      aactgtatc aacgtttac gagattgcg attgtcgta 
      acgctagtc aaggcactg atactgggt ggtgttgca 
      agcagcgac atcggcgtc attgcgaca tacagggcc 
      caggtgtcg caaatcacc gatttgctc gaaaaatat 
      caagtcgat gttagcaca gtggaccag tttcaaggg 
      cgcgataaa agtgtgatt atttattct tgtaccaaa 
      agttcggcc caagttgca acaaatacg cttaaatac 
      gaattgttg gaggataag aggagactg actgttgct 
      gtgactcga gccaaacgt aaattacta atagttggg 
      gattccagc actttggtg aggtacaag acgtttgag 
      acgatttta actcacctg gaaaaacat atcgttaag 
      tttgattca gagtggtcc attttgaat agtctctaa
    mRNA sequence
    • >TC000140 RA
      acataaaaa taacctcac aattttaca aaagcccgc 
      caataacat ttttgcatt aattatgaa aaaaactaa 
      agttcttaa cgcctctaa acacaattg gaaaatttc 
      cgatttttt tacaaaatg tcccaaccc ttcacggac 
      tgtaagctg tgaaaacaa aaggaaaaa ttccgattt 
      tcccataaa gactgagcc ccaatcacc cgaaaaatg 
      taacgcgga gtcaccaaa tagcaaacg aagaaaatt 
      cgatgaaaa tagcgatcc tttagattc tcacaatat 
      aacaatcaa gattgaacc cttatctca agtttgtga 
      cagtggtgg tactagttt taacgtcgt aacacctga 
      aaaaaaatt caaaccttc cagttccat gaagacacc 
      cacccctaa aaaaatcga gattttggc cagttctaa 
      tcaaaggag gacgcccca aagtgggcg gaaaacaac 
      gccaaagaa attatttga aaaatcgcc gaggaaatt 
      ggaggaatt taacgtcca aattttgga aaatttcgt 
      caaagtgtc tccgagcac ttccaacag aaaaatcgt 
      aattaaaag cacgccgac caaaaacca tgcgcagag 
      tcacatcga tgaattttt atctccaac tatttcaaa 
      gtctcctgc ccaaagtcg caatgaaac cactccgtc 
      caaagccag aactcggtt atatttcgt ggatgataa 
      aaaaccgag tccaatgga tgatttaga tcaattttt 
      taacgattc gtttgagga tttagttat taattgcaa 
      tacattgga tttggggga accacagca ttgtcagat 
      catccaagt gtcacacaa tggaggcaa aatcgtctt 
      gaatttaaa atcgaccaa gggcgggga gactggttt 
      ttgttacat tgaacaatt ttgggcttc cggaaaccg 
      attcaaaac cggcgattc tgtctacat caaggcgga 
      aaaaaactc aaacggtga ttgggtcgt taacaacga 
      cacgggatg gctagttta ccaaccaga tatattaat 
      ttcgtgcac ttcagtcgt cggaagtct attttgcaa 
      acgcaaagc tgtcttatc cgaccgttt ccgtggctt 
      cgaaccaac aaaccgcgt catgctaat cggcacttt 
      agcacacac cctcctcca acaatcttt gagaaaaaa 
      attaacaaa aaggaagca aattgaggc gattttgaa 
      aaatttgct cgcatccaa agacacgat aaaaagatt 
      gtacgaagc caacctaac cgttgccga ccttgaatc 
      agacttgga ggtgtttgt gcccaaatt ggtcgcttt 
      tatcagcga acacataac aacggggca aatctgcga 
      cattgagga tattgagga gaatttgtg gtgccctca 
      gttggggct caagggcaa aatcgatgt tacagtgcg 
      gtcaaatac cggcgttat gcccctcga ggtcaagac 
      aggtcgagc gactgtctc acttgagca cagaggaca 
      agtcgtttt atacttaat gatgatgaa taagttcgg 
      gcttgatgt ctcttctgg tctgttgct ctacttgaa 
      agaaggggt tttgaagga aattaaggc cacagagca 
      agaaaaacg tgatgtttt gattttacg gaacgaatt 
      ggcgtattt tttggccag tgacgtagg ggaattacc 
      ggagccgtt gaataatat tcgggtttg tcagaagtg 
      tgctttgag taccgtttg cagcgccta tttgaagtt 
      tgaagatca cgatttaac agaaaataa agcgatgca 
      agaagtaca aaagaaatc aattggtca tttgagtca 
      agcacactt agattattt tctcaaatg gagtaattt 
      gttgagcat ggaagcaca acatagtaa aacaacaaa 
      gtcgctaca acaaattta cactttacc accggctaa 
      aagagaagc tttaggtcg atcgattgt caacatgag 
      agtgacttt tgtcgaaaa tgaaaccaa cggtttatt 
      taaacacac aatggagaa ggaaaatcc cagtttggc 
      caaccttac ggcttacga tatcattga aaataatta 
      catagtcgt cagcactga ttcccggcc tgctgttgc 
      gtcaggatt cgtaacaga agtctcccc aactcaaat 
      cagtattac acttgatag agatctcaa tgtaaaata 
      tcgcaatca attgtttca tctcgacac ctacgattc 
      aaatatcac ttttacttt taatatgac aagtttagt 
      gttgcttat tgagaattc ggaaagggc tgctcagtt 
      aaggagtgt tataattga taaagctcc gcccacttt 
      tgcaccaaa actgccgtc agttgttgc gaaaaaggg 
      ggccccgat tttacgacg tctaaatca tgtgcaaca 
      gaaggctgt gcttaaagc attgtgtgc aaatcagtt 
      tttgctgat caaggggat gccaggcac tggtaaaag 
      cgccaccat tgttgccct aatccaact gctaatcga 
      actgaataa aagtgtcct cataactag tcatactca 
      ttcagccat tgacaacat ttgtatccg tttgcagaa 
      actaaacgt tgacttcat aagactcgg atctgagag 
      taaaatcga ttcgaaatt gaaaccaaa tttggaaac 
      gactctcac tgaaaaatg tacaacacc cgaagagat 
      gaacgaaat ttacaacaa atacaaagt gattgcggt 
      tacgtgtct cggctccgg ccaccctct cctcaccaa 
      acgcactgt agacgtatg tatagtcga tgaaagcac 
      tcaagtcct ccaaacgtc agtgattaa gccgttatt 
      tgctgccaa atcttttat tctgattgg tgaccccca 
      ccaacttgc acctctagt acgccaacg tgatgcctt 
      agaggcagg aatgtcgga aagtctttt cgaaagact 
      ggaaaattc tgaagggac aattaccct aaacctcaa 
      ctatcgcat gaataaaac aatcacgga gttggccaa 
      tgctctcac gtatcaagg ccaactaca gacggccag 
      tgaagaagt ggcaaaccg tgtcttgaa actgcccaa 
      aattgagat cgttaaaaa agagtacaa agagcacca 
      gtggctgct caactgcct cgatgtgtc gttagaggg 
      cgctgtaca atttatcga caccggccc tgtatccga 
      attaaccaa tcacgctga ttggattac caaaactgt 
      gaaagacgg ttgtgatct gcagaactg tatcaacgt 
      ttacgagat tgcgattgt cgtaacgct agtcaaggc 
      actgatact gggtggtgt tgcaagcag cgacatcgg 
      cgtcattgc gacatacag ggcccaggt gtcgcaaat 
      caccgattt gctcgaaaa atatcaagt cgatgttag 
      cacagtgga ccagtttca agggcgcga taaaagtgt 
      gattattta ttcttgtac caaaagttc ggcccaagt 
      tgcaacaaa tacgcttaa atacgaatt gttggagga 
      taagaggag actgactgt tgctgtgac tcgagccaa 
      acgtaaatt actaatagt tggggattc cagcacttt 
      ggtgaggta caagacgtt tgagacgat tttaactca 
      cctggaaaa acatatcgt taagtttga ttcagagtg 
      gtccatttt gaatagtct ctaagtatt gtttttgag 
      gattattta tttttttaa gccagaagg aaggaatcg 
      tggttaagt gagaatttc tatttttta actttataa 
      ataaaaaaa tcctaatat a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY