Status
Please wait ...
Gene information
General information
TC011452 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6246540..6249737
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011452
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07994

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011452 RA
      MSESEEETS SAADWPVTE EWLQAVLRH HHKHLEDDA 
      AAITVVDFT VKPGCDAGE SVLSDILAV AVKYCLKDD 
      PVAHCLSFI IKLLPQDPF SRYFVTEAQ FDLREIKFY 
      TQVVPDLES FKSQHLAPA SPLPLPIPQ CYYAHYSPG 
      SPEPEPSPP ESVLVLENI KPSGYSSAD FSRGLTLRQ 
      TTSAVDAIA RIHGLSLAL KIKEGKSLV ERYPFLFQT 
      TRASDSYQQ LVERGLPQL SQFLERRPG QEGVLAALN 
      DLRPHTKDI IENLLAPEE PMALITHTD FWCNNLMFQ 
      EDENVSTCA ILDWQMVTY SRATNDLAL LLISSVPAE 
      LRRLHTEKL LDGYWTNLT ETCCRFKLD VEKELGYNR 
      KQLGEDYKR SQLLALLLC IGSVDLALG NAQMEERLL 
      ALLQDLYDE GLLNADVAK K
    CDS sequence
    • >TC011452 RA
      atgtcggaa agcgaggaa gagacgagc agcgccgcc 
      gactggccc gtcaccgag gaatggctc caggccgtc 
      ctccggcac caccacaag cacctggag gacgacgcc 
      gccgccatc accgtcgtc gatttcaca gtgaaaccg 
      ggctgtgac gccggcgag agcgtcctc agtgatatt 
      ctcgccgtc gccgtcaaa tactgcctc aaagacgac 
      ccagtcgcg cactgtctc agcttcatc atcaaactc 
      ctgccccag gaccccttc agtcggtat ttcgtcacc 
      gaagcacaa ttcgatctc agagaaatc aagttctac 
      acgcaagtt gtgcccgac ttggaatcg ttcaaatcg 
      caacacctc gcgcccgcc tcccccctg ccgctcccc 
      atcccgcaa tgctactac gcgcactac tcccccggg 
      agccccgag cccgagccg agtccccct gaatccgtc 
      ctcgtcctg gagaacatc aaaccttcc ggttacagc 
      agtgccgat ttttcgcgc ggtttaacc ctccgtcag 
      acgacatcg gccgtcgat gccatcgct cgcatccat 
      ggcctctcc ctcgccctt aaaatcaaa gagggaaaa 
      tcgctcgtg gaacgctac ccttttctc ttccaaacg 
      actcgcgct tcggactcg taccaacaa ctcgtcgaa 
      cgcggtctc ccccaacta tcacaattc ctcgaacgc 
      cgccccggc caggagggg gtgctggcc gcactgaac 
      gacctccga ccccacacc aaagacatc atcgagaat 
      ctgctcgcg cccgaagaa ccaatggct ctgatcacg 
      cacacggac ttctggtgc aataatctc atgttccag 
      gaggatgaa aatgtgagc acttgtgcc attttggat 
      tggcagatg gtgacgtat agtcgggca accaatgat 
      ttggctttg ttgctcatc agttcggtg ccggccgag 
      ctgagacga ttacacacg gagaagttg ctcgatggt 
      tattggacg aatttgacg gagacttgt tgtcggttc 
      aagttggat gtggagaag gaactcggt tataaccgg 
      aaacaattg ggtgaggat tataaacgg tcgcaactt 
      ttggctttg ctcctttgt atcggttcg gtggatttg 
      gctttggga aatgctcag atggaagaa cggcttctt 
      gcactgcta caggacctt tacgacgaa gggctgctt 
      aatgccgac gtggccaaa aaataa
    mRNA sequence
    • >TC011452 RA
      ctcctagca tgacgcagt gatgtcgga aagcgagga 
      agagacgag cagcgccgc cgactggcc cgtcaccga 
      ggaatggct ccaggccgt cctccggca ccaccacaa 
      gcacctgga ggacgacgc cgccgccat caccgtcgt 
      cgatttcac agtgaaacc gggctgtga cgccggcga 
      gagcgtcct cagtgatat tctcgccgt cgccgtcaa 
      atactgcct caaagacga cccagtcgc gcactgtct 
      cagcttcat catcaaact cctgcccca ggacccctt 
      cagtcggta tttcgtcac cgaagcaca attcgatct 
      cagagaaat caagttcta cacgcaagt tgtgcccga 
      cttggaatc gttcaaatc gcaacacct cgcgcccgc 
      ctcccccct gccgctccc catcccgca atgctacta 
      cgcgcacta ctcccccgg gagccccga gcccgagcc 
      gagtccccc tgaatccgt cctcgtcct ggagaacat 
      caaaccttc cggttacag cagtgccga tttttcgcg 
      cggtttaac cctccgtca gacgacatc ggccgtcga 
      tgccatcgc tcgcatcca tggcctctc cctcgccct 
      taaaatcaa agagggaaa atcgctcgt ggaacgcta 
      cccttttct cttccaaac gactcgcgc ttcggactc 
      gtaccaaca actcgtcga acgcggtct cccccaact 
      atcacaatt cctcgaacg ccgccccgg ccaggaggg 
      ggtgctggc cgcactgaa cgacctccg accccacac 
      caaagacat catcgagaa tctgctcgc gcccgaaga 
      accaatggc tctgatcac gcacacgga cttctggtg 
      caataatct catgttcca ggaggatga aaatgtgag 
      cacttgtgc cattttgga ttggcagat ggtgacgta 
      tagtcgggc aaccaatga tttggcttt gttgctcat 
      cagttcggt gccggccga gctgagacg attacacac 
      ggagaagtt gctcgatgg ttattggac gaatttgac 
      ggagacttg ttgtcggtt caagttgga tgtggagaa 
      ggaactcgg ttataaccg gaaacaatt gggtgagga 
      ttataaacg gtcgcaact tttggcttt gctcctttg 
      tatcggttc ggtggattt ggctttggg aaatgctca 
      gatggaaga acggcttct tgcactgct acaggacct 
      ttacgacga agggctgct taatgccga cgtggccaa 
      aaaataaca cgaaatgtg gatgcgatt caatcggct 
      agtgacttt gttcttgct tcgaaatgt gttccgtgc 
      caattttac gttcagttg tagaataac taaatgatt 
      gtgtatttt gtaacgatg tgcgatgac ttttggtca 
      ataaaagag aaagaaact t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY