Status
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Gene information
General information
TC000103 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11124837..11185634
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000103
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07993

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000103 RA
      MSSNNQKLS TTQRMIQAV PFPPSHKLT VAEVFDQRT 
      AKPRPDVLK QHFILEGRI DEAAALRII NEGAQLLRA 
      EKTMIDIEA PVTVCGDIH GQFYDLMKL FEVGGPPAS 
      TKYLFLGDY VDRGYFSIE CVLYLWALK LCYPTTLFL 
      LRGNHECRH LTEYFTFKQ ECKIKYSER VYDACMEAF 
      DCLPLAALM NQQFLCVHG GLSPEIHNL DDIRKLDRF 
      KEPPAFGPM CDLLWSDPL EDFGNENNA EHFSHNSVR 
      GCSYFYSYA ACCDFLQNN NLLSIIRAH EAQDAGYRM 
      YRKSQTTGF PSLITIFSA PNYLDVYNN KAAVLKYEN 
      NVMNIRQFN CSAHPYWLP NFMDVFTWS LPFVGEKVT 
      EMLVNVLNI CSDDELVSD GDDALEEAA NLRKEVIRN 
      KIRAIGKMA RVFSVLREE SESVLQLKG LTPTGALPL 
      GALSGGKTS LKNALQGFS PNHKITSFA EAKGLDAIN 
      ERMPPRKDA PPSPAEETP QDHNHTQSN AHS
    • >TC000103 RB
      MSSNNQKLS TTQRMIQAV PFPPSHKLT VAEVFDQRT 
      AKPRPDVLK QHFILEGRI DEAAALRII NEGAQLLRA 
      EKTMIDIEA PVTVCGDIH GQFYDLMKL FEVGGPPAS 
      TKYLFLGDY VDRGYFSIE CVLYLWALK LCYPTTLFL 
      LRGNHECRH LTEYFTFKQ ECKIKYSER VYDACMEAF 
      DCLPLAALM NQQFLCVHG GLSPEIHNL DDIRKLDRF 
      KEPPAFGPM CDLLWSDPL EDFGNENNA EHFSHNSVR 
      GCSYFYSYA ACCDFLQNN NLLSIIRAH EAQDAGYRM 
      YRKSQTTGF PSLITIFSA PNYLDVYNN KAAVLKYEN 
      NVMNIRQFN CSAHPYWLP NFMDVFTWS LPFVGEKVT 
      EMLVNVLNI CSDDELVSD GDDALEEAN LRKEVIRNK 
      IRAIGKMAR VFSVLREES ESVLQLKGL TPTGALPLG 
      ALSGGKTSL KNALQGFSP NHKITSFAE AKGLDAINE 
      RMPPRKDAP PSPAEETPQ DHNHTQSNA HS
    CDS sequence
    • >TC000103 RA
      atgtcttcg aacaaccag aagctatcg accacgcaa 
      cgtatgata caagctgtc ccgtttcca ccgagtcat 
      aagttgaca gtggccgaa gtgtttgac cagcggaca 
      gcaaaaccg cgaccggac gtgttaaag cagcacttt 
      atattggag ggtcgaatc gatgaagca gcagctctc 
      cgaatcatc aatgaaggc gcacaactc ctacgcgcc 
      gaaaagacg atgatagat atagaagct cccgtcaca 
      gtatgtgga gatatacac ggtcaattt tatgacctt 
      atgaaatta tttgaagta gggggacca cccgctagt 
      acaaagtac ctttttctc ggtgattat gttgataga 
      ggatatttt agtatagag tgcgtgttg tacctgtgg 
      gccctgaag ctgtgctac cctacgacc ttattcctt 
      cttcgtggt aaccatgag tgtcgccac cttacagaa 
      tatttcact tttaaacaa gaatgtaag ataaaatat 
      tcagaacgt gtatatgat gcgtgtatg gaggcattc 
      gactgcctg cctcttgcg gcgctgatg aaccaacag 
      ttcctctgc gtccatggc ggattatct ccagaaata 
      cataattta gatgacatt cgaaagtta gacagattt 
      aaggaaccg cccgctttc gggccgatg tgcgacctc 
      ctgtggtct gacccttta gaagacttc ggtaacgaa 
      aacaacgca gaacatttc tctcataat tccgtgagg 
      ggttgctca tatttctac agctacgct gcctgctgc 
      gacttccta caaaataat aaccttcta tcaataatt 
      agagcacac gaggcacaa gatgcgggg tatcgaatg 
      tataggaaa agtcaaaca acaggcttc ccttccctt 
      atcacaatt ttctcagca cccaattac ctggacgtg 
      tataataat aaggcagcc gtactaaag tatgaaaat 
      aacgtaatg aacattcgc caatttaat tgttcggcg 
      cacccttac tggttacca aactttatg gatgtgttc 
      acgtggtcg cttcctttc gttggggaa aaagtgacg 
      gaaatgctc gtgaacgtg ctcaacatc tgttctgat 
      gacgaactc gtctcagac ggtgatgat gcgctagaa 
      gaagcagca aacttgagg aaagaagtg atacgtaac 
      aaaatccgg gcgattggt aaaatggct cgagtattc 
      tctgttcta agggaggaa agtgaatca gtgttgcaa 
      cttaaaggt ctcacgcct acgggtgcg ctgcctcta 
      ggcgcgtta tcaggcgga aagacgagt ctcaaaaac 
      gcgttgcaa ggattctct cctaatcat aagataacg 
      agcttcgcg gaagccaaa ggtttagac gccatcaat 
      gagcgcatg cctccgagg aaagatgcg ccaccgtcg 
      ccggctgaa gaaacgcca caggaccac aatcacacc 
      caatctaat gcacactcg tga
    • >TC000103 RB
      atgtcttcg aacaaccag aagctatcg accacgcaa 
      cgtatgata caagctgtc ccgtttcca ccgagtcat 
      aagttgaca gtggccgaa gtgtttgac cagcggaca 
      gcaaaaccg cgaccggac gtgttaaag cagcacttt 
      atattggag ggtcgaatc gatgaagca gcagctctc 
      cgaatcatc aatgaaggc gcacaactc ctacgcgcc 
      gaaaagacg atgatagat atagaagct cccgtcaca 
      gtatgtgga gatatacac ggtcaattt tatgacctt 
      atgaaatta tttgaagta gggggacca cccgctagt 
      acaaagtac ctttttctc ggtgattat gttgataga 
      ggatatttt agtatagag tgcgtgttg tacctgtgg 
      gccctgaag ctgtgctac cctacgacc ttattcctt 
      cttcgtggt aaccatgag tgtcgccac cttacagaa 
      tatttcact tttaaacaa gaatgtaag ataaaatat 
      tcagaacgt gtatatgat gcgtgtatg gaggcattc 
      gactgcctg cctcttgcg gcgctgatg aaccaacag 
      ttcctctgc gtccatggc ggattatct ccagaaata 
      cataattta gatgacatt cgaaagtta gacagattt 
      aaggaaccg cccgctttc gggccgatg tgcgacctc 
      ctgtggtct gacccttta gaagacttc ggtaacgaa 
      aacaacgca gaacatttc tctcataat tccgtgagg 
      ggttgctca tatttctac agctacgct gcctgctgc 
      gacttccta caaaataat aaccttcta tcaataatt 
      agagcacac gaggcacaa gatgcgggg tatcgaatg 
      tataggaaa agtcaaaca acaggcttc ccttccctt 
      atcacaatt ttctcagca cccaattac ctggacgtg 
      tataataat aaggcagcc gtactaaag tatgaaaat 
      aacgtaatg aacattcgc caatttaat tgttcggcg 
      cacccttac tggttacca aactttatg gatgtgttc 
      acgtggtcg cttcctttc gttggggaa aaagtgacg 
      gaaatgctc gtgaacgtg ctcaacatc tgttctgat 
      gacgaactc gtctcagac ggtgatgat gcgctagaa 
      gaagcaaac ttgaggaaa gaagtgata cgtaacaaa 
      atccgggcg attggtaaa atggctcga gtattctct 
      gttctaagg gaggaaagt gaatcagtg ttgcaactt 
      aaaggtctc acgcctacg ggtgcgctg cctctaggc 
      gcgttatca ggcggaaag acgagtctc aaaaacgcg 
      ttgcaagga ttctctcct aatcataag ataacgagc 
      ttcgcggaa gccaaaggt ttagacgcc atcaatgag 
      cgcatgcct ccgaggaaa gatgcgcca ccgtcgccg 
      gctgaagaa acgccacag gaccacaat cacacccaa 
      tctaatgca cactcgtga
    mRNA sequence
    • >TC000103 RA
      cgcgctcgt cgctccatc ttgagtaga atatagctt 
      gtgaagggt gagtgtata attgtgcga cgaaaatcc 
      gatgtttat agtttaaag tgtataaaa ttacttcaa 
      gaaatgtct tcgaacaac cagaagcta tcgaccacg 
      caacgtatg atacaagct gtcccgttt ccaccgagt 
      cataagttg acagtggcc gaagtgttt gaccagcgg 
      acagcaaaa ccgcgaccg gacgtgtta aagcagcac 
      tttatattg gagggtcga atcgatgaa gcagcagct 
      ctccgaatc atcaatgaa ggcgcacaa ctcctacgc 
      gccgaaaag acgatgata gatatagaa gctcccgtc 
      acagtatgt ggagatata cacggtcaa ttttatgac 
      cttatgaaa ttatttgaa gtaggggga ccacccgct 
      agtacaaag taccttttt ctcggtgat tatgttgat 
      agaggatat tttagtata gagtgcgtg ttgtacctg 
      tgggccctg aagctgtgc taccctacg accttattc 
      cttcttcgt ggtaaccat gagtgtcgc caccttaca 
      gaatatttc acttttaaa caagaatgt aagataaaa 
      tattcagaa cgtgtatat gatgcgtgt atggaggca 
      ttcgactgc ctgcctctt gcggcgctg atgaaccaa 
      cagttcctc tgcgtccat ggcggatta tctccagaa 
      atacataat ttagatgac attcgaaag ttagacaga 
      tttaaggaa ccgcccgct ttcgggccg atgtgcgac 
      ctcctgtgg tctgaccct ttagaagac ttcggtaac 
      gaaaacaac gcagaacat ttctctcat aattccgtg 
      aggggttgc tcatatttc tacagctac gctgcctgc 
      tgcgacttc ctacaaaat aataacctt ctatcaata 
      attagagca cacgaggca caagatgcg gggtatcga 
      atgtatagg aaaagtcaa acaacaggc ttcccttcc 
      cttatcaca attttctca gcacccaat tacctggac 
      gtgtataat aataaggca gccgtacta aagtatgaa 
      aataacgta atgaacatt cgccaattt aattgttcg 
      gcgcaccct tactggtta ccaaacttt atggatgtg 
      ttcacgtgg tcgcttcct ttcgttggg gaaaaagtg 
      acggaaatg ctcgtgaac gtgctcaac atctgttct 
      gatgacgaa ctcgtctca gacggtgat gatgcgcta 
      gaagaagca gcaaacttg aggaaagaa gtgatacgt 
      aacaaaatc cgggcgatt ggtaaaatg gctcgagta 
      ttctctgtt ctaagggag gaaagtgaa tcagtgttg 
      caacttaaa ggtctcacg cctacgggt gcgctgcct 
      ctaggcgcg ttatcaggc ggaaagacg agtctcaaa 
      aacgcgttg caaggattc tctcctaat cataagata 
      acgagcttc gcggaagcc aaaggttta gacgccatc 
      aatgagcgc atgcctccg aggaaagat gcgccaccg 
      tcgccggct gaagaaacg ccacaggac cacaatcac 
      acccaatct aatgcacac tcgtgagct acacttaaa 
      taggacatc acattggcc ggctgcagg tttgtgcat 
      tagttt
    • >TC000103 RB
      cgcgctcgt cgctccatc ttgagtaga atatagctt 
      gtgaagggt gagtgtata attgtgcga cgaaaatcc 
      gatgtttat agtttaaag tgtataaaa ttacttcaa 
      gaaatgtct tcgaacaac cagaagcta tcgaccacg 
      caacgtatg atacaagct gtcccgttt ccaccgagt 
      cataagttg acagtggcc gaagtgttt gaccagcgg 
      acagcaaaa ccgcgaccg gacgtgtta aagcagcac 
      tttatattg gagggtcga atcgatgaa gcagcagct 
      ctccgaatc atcaatgaa ggcgcacaa ctcctacgc 
      gccgaaaag acgatgata gatatagaa gctcccgtc 
      acagtatgt ggagatata cacggtcaa ttttatgac 
      cttatgaaa ttatttgaa gtaggggga ccacccgct 
      agtacaaag taccttttt ctcggtgat tatgttgat 
      agaggatat tttagtata gagtgcgtg ttgtacctg 
      tgggccctg aagctgtgc taccctacg accttattc 
      cttcttcgt ggtaaccat gagtgtcgc caccttaca 
      gaatatttc acttttaaa caagaatgt aagataaaa 
      tattcagaa cgtgtatat gatgcgtgt atggaggca 
      ttcgactgc ctgcctctt gcggcgctg atgaaccaa 
      cagttcctc tgcgtccat ggcggatta tctccagaa 
      atacataat ttagatgac attcgaaag ttagacaga 
      tttaaggaa ccgcccgct ttcgggccg atgtgcgac 
      ctcctgtgg tctgaccct ttagaagac ttcggtaac 
      gaaaacaac gcagaacat ttctctcat aattccgtg 
      aggggttgc tcatatttc tacagctac gctgcctgc 
      tgcgacttc ctacaaaat aataacctt ctatcaata 
      attagagca cacgaggca caagatgcg gggtatcga 
      atgtatagg aaaagtcaa acaacaggc ttcccttcc 
      cttatcaca attttctca gcacccaat tacctggac 
      gtgtataat aataaggca gccgtacta aagtatgaa 
      aataacgta atgaacatt cgccaattt aattgttcg 
      gcgcaccct tactggtta ccaaacttt atggatgtg 
      ttcacgtgg tcgcttcct ttcgttggg gaaaaagtg 
      acggaaatg ctcgtgaac gtgctcaac atctgttct 
      gatgacgaa ctcgtctca gacggtgat gatgcgcta 
      gaagaagca aacttgagg aaagaagtg atacgtaac 
      aaaatccgg gcgattggt aaaatggct cgagtattc 
      tctgttcta agggaggaa agtgaatca gtgttgcaa 
      cttaaaggt ctcacgcct acgggtgcg ctgcctcta 
      ggcgcgtta tcaggcgga aagacgagt ctcaaaaac 
      gcgttgcaa ggattctct cctaatcat aagataacg 
      agcttcgcg gaagccaaa ggtttagac gccatcaat 
      gagcgcatg cctccgagg aaagatgcg ccaccgtcg 
      ccggctgaa gaaacgcca caggaccac aatcacacc 
      caatctaat gcacactcg tgagctaca cttaaatag 
      gacatcaca ttggccggc tgcaggttt gtgcattag 
      ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY