Status
Please wait ...
Gene information
General information
TC000103 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11124837..11185634
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000103
    iBeetle screen iB_07993

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000103 RA
      MSSNNQKLS TTQRMIQAV PFPPSHKLT VAEVFDQRT 
AKPRPDVLK QHFILEGRI DEAAALRII NEGAQLLRA 
EKTMIDIEA PVTVCGDIH GQFYDLMKL FEVGGPPAS 
TKYLFLGDY VDRGYFSIE CVLYLWALK LCYPTTLFL 
LRGNHECRH LTEYFTFKQ ECKIKYSER VYDACMEAF 
DCLPLAALM NQQFLCVHG GLSPEIHNL DDIRKLDRF 
KEPPAFGPM CDLLWSDPL EDFGNENNA EHFSHNSVR 
GCSYFYSYA ACCDFLQNN NLLSIIRAH EAQDAGYRM 
YRKSQTTGF PSLITIFSA PNYLDVYNN KAAVLKYEN 
NVMNIRQFN CSAHPYWLP NFMDVFTWS LPFVGEKVT 
EMLVNVLNI CSDDELVSD GDDALEEAA NLRKEVIRN 
KIRAIGKMA RVFSVLREE SESVLQLKG LTPTGALPL 
GALSGGKTS LKNALQGFS PNHKITSFA EAKGLDAIN 
ERMPPRKDA PPSPAEETP QDHNHTQSN AHS
    • >TC000103 RB
      MSSNNQKLS TTQRMIQAV PFPPSHKLT VAEVFDQRT 
AKPRPDVLK QHFILEGRI DEAAALRII NEGAQLLRA 
EKTMIDIEA PVTVCGDIH GQFYDLMKL FEVGGPPAS 
TKYLFLGDY VDRGYFSIE CVLYLWALK LCYPTTLFL 
LRGNHECRH LTEYFTFKQ ECKIKYSER VYDACMEAF 
DCLPLAALM NQQFLCVHG GLSPEIHNL DDIRKLDRF 
KEPPAFGPM CDLLWSDPL EDFGNENNA EHFSHNSVR 
GCSYFYSYA ACCDFLQNN NLLSIIRAH EAQDAGYRM 
YRKSQTTGF PSLITIFSA PNYLDVYNN KAAVLKYEN 
NVMNIRQFN CSAHPYWLP NFMDVFTWS LPFVGEKVT 
EMLVNVLNI CSDDELVSD GDDALEEAN LRKEVIRNK 
IRAIGKMAR VFSVLREES ESVLQLKGL TPTGALPLG 
ALSGGKTSL KNALQGFSP NHKITSFAE AKGLDAINE 
RMPPRKDAP PSPAEETPQ DHNHTQSNA HS
    CDS sequence
    • >TC000103 RA
      atgtcttcg aacaaccag aagctatcg accacgcaa 
cgtatgata caagctgtc ccgtttcca ccgagtcat 
aagttgaca gtggccgaa gtgtttgac cagcggaca 
gcaaaaccg cgaccggac gtgttaaag cagcacttt 
atattggag ggtcgaatc gatgaagca gcagctctc 
cgaatcatc aatgaaggc gcacaactc ctacgcgcc 
gaaaagacg atgatagat atagaagct cccgtcaca 
gtatgtgga gatatacac ggtcaattt tatgacctt 
atgaaatta tttgaagta gggggacca cccgctagt 
acaaagtac ctttttctc ggtgattat gttgataga 
ggatatttt agtatagag tgcgtgttg tacctgtgg 
gccctgaag ctgtgctac cctacgacc ttattcctt 
cttcgtggt aaccatgag tgtcgccac cttacagaa 
tatttcact tttaaacaa gaatgtaag ataaaatat 
tcagaacgt gtatatgat gcgtgtatg gaggcattc 
gactgcctg cctcttgcg gcgctgatg aaccaacag 
ttcctctgc gtccatggc ggattatct ccagaaata 
cataattta gatgacatt cgaaagtta gacagattt 
aaggaaccg cccgctttc gggccgatg tgcgacctc 
ctgtggtct gacccttta gaagacttc ggtaacgaa 
aacaacgca gaacatttc tctcataat tccgtgagg 
ggttgctca tatttctac agctacgct gcctgctgc 
gacttccta caaaataat aaccttcta tcaataatt 
agagcacac gaggcacaa gatgcgggg tatcgaatg 
tataggaaa agtcaaaca acaggcttc ccttccctt 
atcacaatt ttctcagca cccaattac ctggacgtg 
tataataat aaggcagcc gtactaaag tatgaaaat 
aacgtaatg aacattcgc caatttaat tgttcggcg 
cacccttac tggttacca aactttatg gatgtgttc 
acgtggtcg cttcctttc gttggggaa aaagtgacg 
gaaatgctc gtgaacgtg ctcaacatc tgttctgat 
gacgaactc gtctcagac ggtgatgat gcgctagaa 
gaagcagca aacttgagg aaagaagtg atacgtaac 
aaaatccgg gcgattggt aaaatggct cgagtattc 
tctgttcta agggaggaa agtgaatca gtgttgcaa 
cttaaaggt ctcacgcct acgggtgcg ctgcctcta 
ggcgcgtta tcaggcgga aagacgagt ctcaaaaac 
gcgttgcaa ggattctct cctaatcat aagataacg 
agcttcgcg gaagccaaa ggtttagac gccatcaat 
gagcgcatg cctccgagg aaagatgcg ccaccgtcg 
ccggctgaa gaaacgcca caggaccac aatcacacc 
caatctaat gcacactcg tga
    • >TC000103 RB
      atgtcttcg aacaaccag aagctatcg accacgcaa 
cgtatgata caagctgtc ccgtttcca ccgagtcat 
aagttgaca gtggccgaa gtgtttgac cagcggaca 
gcaaaaccg cgaccggac gtgttaaag cagcacttt 
atattggag ggtcgaatc gatgaagca gcagctctc 
cgaatcatc aatgaaggc gcacaactc ctacgcgcc 
gaaaagacg atgatagat atagaagct cccgtcaca 
gtatgtgga gatatacac ggtcaattt tatgacctt 
atgaaatta tttgaagta gggggacca cccgctagt 
acaaagtac ctttttctc ggtgattat gttgataga 
ggatatttt agtatagag tgcgtgttg tacctgtgg 
gccctgaag ctgtgctac cctacgacc ttattcctt 
cttcgtggt aaccatgag tgtcgccac cttacagaa 
tatttcact tttaaacaa gaatgtaag ataaaatat 
tcagaacgt gtatatgat gcgtgtatg gaggcattc 
gactgcctg cctcttgcg gcgctgatg aaccaacag 
ttcctctgc gtccatggc ggattatct ccagaaata 
cataattta gatgacatt cgaaagtta gacagattt 
aaggaaccg cccgctttc gggccgatg tgcgacctc 
ctgtggtct gacccttta gaagacttc ggtaacgaa 
aacaacgca gaacatttc tctcataat tccgtgagg 
ggttgctca tatttctac agctacgct gcctgctgc 
gacttccta caaaataat aaccttcta tcaataatt 
agagcacac gaggcacaa gatgcgggg tatcgaatg 
tataggaaa agtcaaaca acaggcttc ccttccctt 
atcacaatt ttctcagca cccaattac ctggacgtg 
tataataat aaggcagcc gtactaaag tatgaaaat 
aacgtaatg aacattcgc caatttaat tgttcggcg 
cacccttac tggttacca aactttatg gatgtgttc 
acgtggtcg cttcctttc gttggggaa aaagtgacg 
gaaatgctc gtgaacgtg ctcaacatc tgttctgat 
gacgaactc gtctcagac ggtgatgat gcgctagaa 
gaagcaaac ttgaggaaa gaagtgata cgtaacaaa 
atccgggcg attggtaaa atggctcga gtattctct 
gttctaagg gaggaaagt gaatcagtg ttgcaactt 
aaaggtctc acgcctacg ggtgcgctg cctctaggc 
gcgttatca ggcggaaag acgagtctc aaaaacgcg 
ttgcaagga ttctctcct aatcataag ataacgagc 
ttcgcggaa gccaaaggt ttagacgcc atcaatgag 
cgcatgcct ccgaggaaa gatgcgcca ccgtcgccg 
gctgaagaa acgccacag gaccacaat cacacccaa 
tctaatgca cactcgtga
    mRNA sequence
    • >TC000103 RA
      cgcgctcgt cgctccatc ttgagtaga atatagctt 
gtgaagggt gagtgtata attgtgcga cgaaaatcc 
gatgtttat agtttaaag tgtataaaa ttacttcaa 
gaaatgtct tcgaacaac cagaagcta tcgaccacg 
caacgtatg atacaagct gtcccgttt ccaccgagt 
cataagttg acagtggcc gaagtgttt gaccagcgg 
acagcaaaa ccgcgaccg gacgtgtta aagcagcac 
tttatattg gagggtcga atcgatgaa gcagcagct 
ctccgaatc atcaatgaa ggcgcacaa ctcctacgc 
gccgaaaag acgatgata gatatagaa gctcccgtc 
acagtatgt ggagatata cacggtcaa ttttatgac 
cttatgaaa ttatttgaa gtaggggga ccacccgct 
agtacaaag taccttttt ctcggtgat tatgttgat 
agaggatat tttagtata gagtgcgtg ttgtacctg 
tgggccctg aagctgtgc taccctacg accttattc 
cttcttcgt ggtaaccat gagtgtcgc caccttaca 
gaatatttc acttttaaa caagaatgt aagataaaa 
tattcagaa cgtgtatat gatgcgtgt atggaggca 
ttcgactgc ctgcctctt gcggcgctg atgaaccaa 
cagttcctc tgcgtccat ggcggatta tctccagaa 
atacataat ttagatgac attcgaaag ttagacaga 
tttaaggaa ccgcccgct ttcgggccg atgtgcgac 
ctcctgtgg tctgaccct ttagaagac ttcggtaac 
gaaaacaac gcagaacat ttctctcat aattccgtg 
aggggttgc tcatatttc tacagctac gctgcctgc 
tgcgacttc ctacaaaat aataacctt ctatcaata 
attagagca cacgaggca caagatgcg gggtatcga 
atgtatagg aaaagtcaa acaacaggc ttcccttcc 
cttatcaca attttctca gcacccaat tacctggac 
gtgtataat aataaggca gccgtacta aagtatgaa 
aataacgta atgaacatt cgccaattt aattgttcg 
gcgcaccct tactggtta ccaaacttt atggatgtg 
ttcacgtgg tcgcttcct ttcgttggg gaaaaagtg 
acggaaatg ctcgtgaac gtgctcaac atctgttct 
gatgacgaa ctcgtctca gacggtgat gatgcgcta 
gaagaagca gcaaacttg aggaaagaa gtgatacgt 
aacaaaatc cgggcgatt ggtaaaatg gctcgagta 
ttctctgtt ctaagggag gaaagtgaa tcagtgttg 
caacttaaa ggtctcacg cctacgggt gcgctgcct 
ctaggcgcg ttatcaggc ggaaagacg agtctcaaa 
aacgcgttg caaggattc tctcctaat cataagata 
acgagcttc gcggaagcc aaaggttta gacgccatc 
aatgagcgc atgcctccg aggaaagat gcgccaccg 
tcgccggct gaagaaacg ccacaggac cacaatcac 
acccaatct aatgcacac tcgtgagct acacttaaa 
taggacatc acattggcc ggctgcagg tttgtgcat 
tagttt
    • >TC000103 RB
      cgcgctcgt cgctccatc ttgagtaga atatagctt 
gtgaagggt gagtgtata attgtgcga cgaaaatcc 
gatgtttat agtttaaag tgtataaaa ttacttcaa 
gaaatgtct tcgaacaac cagaagcta tcgaccacg 
caacgtatg atacaagct gtcccgttt ccaccgagt 
cataagttg acagtggcc gaagtgttt gaccagcgg 
acagcaaaa ccgcgaccg gacgtgtta aagcagcac 
tttatattg gagggtcga atcgatgaa gcagcagct 
ctccgaatc atcaatgaa ggcgcacaa ctcctacgc 
gccgaaaag acgatgata gatatagaa gctcccgtc 
acagtatgt ggagatata cacggtcaa ttttatgac 
cttatgaaa ttatttgaa gtaggggga ccacccgct 
agtacaaag taccttttt ctcggtgat tatgttgat 
agaggatat tttagtata gagtgcgtg ttgtacctg 
tgggccctg aagctgtgc taccctacg accttattc 
cttcttcgt ggtaaccat gagtgtcgc caccttaca 
gaatatttc acttttaaa caagaatgt aagataaaa 
tattcagaa cgtgtatat gatgcgtgt atggaggca 
ttcgactgc ctgcctctt gcggcgctg atgaaccaa 
cagttcctc tgcgtccat ggcggatta tctccagaa 
atacataat ttagatgac attcgaaag ttagacaga 
tttaaggaa ccgcccgct ttcgggccg atgtgcgac 
ctcctgtgg tctgaccct ttagaagac ttcggtaac 
gaaaacaac gcagaacat ttctctcat aattccgtg 
aggggttgc tcatatttc tacagctac gctgcctgc 
tgcgacttc ctacaaaat aataacctt ctatcaata 
attagagca cacgaggca caagatgcg gggtatcga 
atgtatagg aaaagtcaa acaacaggc ttcccttcc 
cttatcaca attttctca gcacccaat tacctggac 
gtgtataat aataaggca gccgtacta aagtatgaa 
aataacgta atgaacatt cgccaattt aattgttcg 
gcgcaccct tactggtta ccaaacttt atggatgtg 
ttcacgtgg tcgcttcct ttcgttggg gaaaaagtg 
acggaaatg ctcgtgaac gtgctcaac atctgttct 
gatgacgaa ctcgtctca gacggtgat gatgcgcta 
gaagaagca aacttgagg aaagaagtg atacgtaac 
aaaatccgg gcgattggt aaaatggct cgagtattc 
tctgttcta agggaggaa agtgaatca gtgttgcaa 
cttaaaggt ctcacgcct acgggtgcg ctgcctcta 
ggcgcgtta tcaggcgga aagacgagt ctcaaaaac 
gcgttgcaa ggattctct cctaatcat aagataacg 
agcttcgcg gaagccaaa ggtttagac gccatcaat 
gagcgcatg cctccgagg aaagatgcg ccaccgtcg 
ccggctgaa gaaacgcca caggaccac aatcacacc 
caatctaat gcacactcg tgagctaca cttaaatag 
gacatcaca ttggccggc tgcaggttt gtgcattag 
ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY