| General information | |||
| TC000103 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 11124837..11185634 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC000103 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07993 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000103 RA
MSSNNQKLS TTQRMIQAV PFPPSHKLT VAEVFDQRT AKPRPDVLK QHFILEGRI DEAAALRII NEGAQLLRA EKTMIDIEA PVTVCGDIH GQFYDLMKL FEVGGPPAS TKYLFLGDY VDRGYFSIE CVLYLWALK LCYPTTLFL LRGNHECRH LTEYFTFKQ ECKIKYSER VYDACMEAF DCLPLAALM NQQFLCVHG GLSPEIHNL DDIRKLDRF KEPPAFGPM CDLLWSDPL EDFGNENNA EHFSHNSVR GCSYFYSYA ACCDFLQNN NLLSIIRAH EAQDAGYRM YRKSQTTGF PSLITIFSA PNYLDVYNN KAAVLKYEN NVMNIRQFN CSAHPYWLP NFMDVFTWS LPFVGEKVT EMLVNVLNI CSDDELVSD GDDALEEAA NLRKEVIRN KIRAIGKMA RVFSVLREE SESVLQLKG LTPTGALPL GALSGGKTS LKNALQGFS PNHKITSFA EAKGLDAIN ERMPPRKDA PPSPAEETP QDHNHTQSN AHS
- >TC000103 RB
MSSNNQKLS TTQRMIQAV PFPPSHKLT VAEVFDQRT AKPRPDVLK QHFILEGRI DEAAALRII NEGAQLLRA EKTMIDIEA PVTVCGDIH GQFYDLMKL FEVGGPPAS TKYLFLGDY VDRGYFSIE CVLYLWALK LCYPTTLFL LRGNHECRH LTEYFTFKQ ECKIKYSER VYDACMEAF DCLPLAALM NQQFLCVHG GLSPEIHNL DDIRKLDRF KEPPAFGPM CDLLWSDPL EDFGNENNA EHFSHNSVR GCSYFYSYA ACCDFLQNN NLLSIIRAH EAQDAGYRM YRKSQTTGF PSLITIFSA PNYLDVYNN KAAVLKYEN NVMNIRQFN CSAHPYWLP NFMDVFTWS LPFVGEKVT EMLVNVLNI CSDDELVSD GDDALEEAN LRKEVIRNK IRAIGKMAR VFSVLREES ESVLQLKGL TPTGALPLG ALSGGKTSL KNALQGFSP NHKITSFAE AKGLDAINE RMPPRKDAP PSPAEETPQ DHNHTQSNA HS
- >TC000103 RA
atgtcttcg aacaaccag aagctatcg accacgcaa cgtatgata caagctgtc ccgtttcca ccgagtcat aagttgaca gtggccgaa gtgtttgac cagcggaca gcaaaaccg cgaccggac gtgttaaag cagcacttt atattggag ggtcgaatc gatgaagca gcagctctc cgaatcatc aatgaaggc gcacaactc ctacgcgcc gaaaagacg atgatagat atagaagct cccgtcaca gtatgtgga gatatacac ggtcaattt tatgacctt atgaaatta tttgaagta gggggacca cccgctagt acaaagtac ctttttctc ggtgattat gttgataga ggatatttt agtatagag tgcgtgttg tacctgtgg gccctgaag ctgtgctac cctacgacc ttattcctt cttcgtggt aaccatgag tgtcgccac cttacagaa tatttcact tttaaacaa gaatgtaag ataaaatat tcagaacgt gtatatgat gcgtgtatg gaggcattc gactgcctg cctcttgcg gcgctgatg aaccaacag ttcctctgc gtccatggc ggattatct ccagaaata cataattta gatgacatt cgaaagtta gacagattt aaggaaccg cccgctttc gggccgatg tgcgacctc ctgtggtct gacccttta gaagacttc ggtaacgaa aacaacgca gaacatttc tctcataat tccgtgagg ggttgctca tatttctac agctacgct gcctgctgc gacttccta caaaataat aaccttcta tcaataatt agagcacac gaggcacaa gatgcgggg tatcgaatg tataggaaa agtcaaaca acaggcttc ccttccctt atcacaatt ttctcagca cccaattac ctggacgtg tataataat aaggcagcc gtactaaag tatgaaaat aacgtaatg aacattcgc caatttaat tgttcggcg cacccttac tggttacca aactttatg gatgtgttc acgtggtcg cttcctttc gttggggaa aaagtgacg gaaatgctc gtgaacgtg ctcaacatc tgttctgat gacgaactc gtctcagac ggtgatgat gcgctagaa gaagcagca aacttgagg aaagaagtg atacgtaac aaaatccgg gcgattggt aaaatggct cgagtattc tctgttcta agggaggaa agtgaatca gtgttgcaa cttaaaggt ctcacgcct acgggtgcg ctgcctcta ggcgcgtta tcaggcgga aagacgagt ctcaaaaac gcgttgcaa ggattctct cctaatcat aagataacg agcttcgcg gaagccaaa ggtttagac gccatcaat gagcgcatg cctccgagg aaagatgcg ccaccgtcg ccggctgaa gaaacgcca caggaccac aatcacacc caatctaat gcacactcg tga
- >TC000103 RB
atgtcttcg aacaaccag aagctatcg accacgcaa cgtatgata caagctgtc ccgtttcca ccgagtcat aagttgaca gtggccgaa gtgtttgac cagcggaca gcaaaaccg cgaccggac gtgttaaag cagcacttt atattggag ggtcgaatc gatgaagca gcagctctc cgaatcatc aatgaaggc gcacaactc ctacgcgcc gaaaagacg atgatagat atagaagct cccgtcaca gtatgtgga gatatacac ggtcaattt tatgacctt atgaaatta tttgaagta gggggacca cccgctagt acaaagtac ctttttctc ggtgattat gttgataga ggatatttt agtatagag tgcgtgttg tacctgtgg gccctgaag ctgtgctac cctacgacc ttattcctt cttcgtggt aaccatgag tgtcgccac cttacagaa tatttcact tttaaacaa gaatgtaag ataaaatat tcagaacgt gtatatgat gcgtgtatg gaggcattc gactgcctg cctcttgcg gcgctgatg aaccaacag ttcctctgc gtccatggc ggattatct ccagaaata cataattta gatgacatt cgaaagtta gacagattt aaggaaccg cccgctttc gggccgatg tgcgacctc ctgtggtct gacccttta gaagacttc ggtaacgaa aacaacgca gaacatttc tctcataat tccgtgagg ggttgctca tatttctac agctacgct gcctgctgc gacttccta caaaataat aaccttcta tcaataatt agagcacac gaggcacaa gatgcgggg tatcgaatg tataggaaa agtcaaaca acaggcttc ccttccctt atcacaatt ttctcagca cccaattac ctggacgtg tataataat aaggcagcc gtactaaag tatgaaaat aacgtaatg aacattcgc caatttaat tgttcggcg cacccttac tggttacca aactttatg gatgtgttc acgtggtcg cttcctttc gttggggaa aaagtgacg gaaatgctc gtgaacgtg ctcaacatc tgttctgat gacgaactc gtctcagac ggtgatgat gcgctagaa gaagcaaac ttgaggaaa gaagtgata cgtaacaaa atccgggcg attggtaaa atggctcga gtattctct gttctaagg gaggaaagt gaatcagtg ttgcaactt aaaggtctc acgcctacg ggtgcgctg cctctaggc gcgttatca ggcggaaag acgagtctc aaaaacgcg ttgcaagga ttctctcct aatcataag ataacgagc ttcgcggaa gccaaaggt ttagacgcc atcaatgag cgcatgcct ccgaggaaa gatgcgcca ccgtcgccg gctgaagaa acgccacag gaccacaat cacacccaa tctaatgca cactcgtga
- >TC000103 RA
cgcgctcgt cgctccatc ttgagtaga atatagctt gtgaagggt gagtgtata attgtgcga cgaaaatcc gatgtttat agtttaaag tgtataaaa ttacttcaa gaaatgtct tcgaacaac cagaagcta tcgaccacg caacgtatg atacaagct gtcccgttt ccaccgagt cataagttg acagtggcc gaagtgttt gaccagcgg acagcaaaa ccgcgaccg gacgtgtta aagcagcac tttatattg gagggtcga atcgatgaa gcagcagct ctccgaatc atcaatgaa ggcgcacaa ctcctacgc gccgaaaag acgatgata gatatagaa gctcccgtc acagtatgt ggagatata cacggtcaa ttttatgac cttatgaaa ttatttgaa gtaggggga ccacccgct agtacaaag taccttttt ctcggtgat tatgttgat agaggatat tttagtata gagtgcgtg ttgtacctg tgggccctg aagctgtgc taccctacg accttattc cttcttcgt ggtaaccat gagtgtcgc caccttaca gaatatttc acttttaaa caagaatgt aagataaaa tattcagaa cgtgtatat gatgcgtgt atggaggca ttcgactgc ctgcctctt gcggcgctg atgaaccaa cagttcctc tgcgtccat ggcggatta tctccagaa atacataat ttagatgac attcgaaag ttagacaga tttaaggaa ccgcccgct ttcgggccg atgtgcgac ctcctgtgg tctgaccct ttagaagac ttcggtaac gaaaacaac gcagaacat ttctctcat aattccgtg aggggttgc tcatatttc tacagctac gctgcctgc tgcgacttc ctacaaaat aataacctt ctatcaata attagagca cacgaggca caagatgcg gggtatcga atgtatagg aaaagtcaa acaacaggc ttcccttcc cttatcaca attttctca gcacccaat tacctggac gtgtataat aataaggca gccgtacta aagtatgaa aataacgta atgaacatt cgccaattt aattgttcg gcgcaccct tactggtta ccaaacttt atggatgtg ttcacgtgg tcgcttcct ttcgttggg gaaaaagtg acggaaatg ctcgtgaac gtgctcaac atctgttct gatgacgaa ctcgtctca gacggtgat gatgcgcta gaagaagca gcaaacttg aggaaagaa gtgatacgt aacaaaatc cgggcgatt ggtaaaatg gctcgagta ttctctgtt ctaagggag gaaagtgaa tcagtgttg caacttaaa ggtctcacg cctacgggt gcgctgcct ctaggcgcg ttatcaggc ggaaagacg agtctcaaa aacgcgttg caaggattc tctcctaat cataagata acgagcttc gcggaagcc aaaggttta gacgccatc aatgagcgc atgcctccg aggaaagat gcgccaccg tcgccggct gaagaaacg ccacaggac cacaatcac acccaatct aatgcacac tcgtgagct acacttaaa taggacatc acattggcc ggctgcagg tttgtgcat tagttt
- >TC000103 RB
cgcgctcgt cgctccatc ttgagtaga atatagctt gtgaagggt gagtgtata attgtgcga cgaaaatcc gatgtttat agtttaaag tgtataaaa ttacttcaa gaaatgtct tcgaacaac cagaagcta tcgaccacg caacgtatg atacaagct gtcccgttt ccaccgagt cataagttg acagtggcc gaagtgttt gaccagcgg acagcaaaa ccgcgaccg gacgtgtta aagcagcac tttatattg gagggtcga atcgatgaa gcagcagct ctccgaatc atcaatgaa ggcgcacaa ctcctacgc gccgaaaag acgatgata gatatagaa gctcccgtc acagtatgt ggagatata cacggtcaa ttttatgac cttatgaaa ttatttgaa gtaggggga ccacccgct agtacaaag taccttttt ctcggtgat tatgttgat agaggatat tttagtata gagtgcgtg ttgtacctg tgggccctg aagctgtgc taccctacg accttattc cttcttcgt ggtaaccat gagtgtcgc caccttaca gaatatttc acttttaaa caagaatgt aagataaaa tattcagaa cgtgtatat gatgcgtgt atggaggca ttcgactgc ctgcctctt gcggcgctg atgaaccaa cagttcctc tgcgtccat ggcggatta tctccagaa atacataat ttagatgac attcgaaag ttagacaga tttaaggaa ccgcccgct ttcgggccg atgtgcgac ctcctgtgg tctgaccct ttagaagac ttcggtaac gaaaacaac gcagaacat ttctctcat aattccgtg aggggttgc tcatatttc tacagctac gctgcctgc tgcgacttc ctacaaaat aataacctt ctatcaata attagagca cacgaggca caagatgcg gggtatcga atgtatagg aaaagtcaa acaacaggc ttcccttcc cttatcaca attttctca gcacccaat tacctggac gtgtataat aataaggca gccgtacta aagtatgaa aataacgta atgaacatt cgccaattt aattgttcg gcgcaccct tactggtta ccaaacttt atggatgtg ttcacgtgg tcgcttcct ttcgttggg gaaaaagtg acggaaatg ctcgtgaac gtgctcaac atctgttct gatgacgaa ctcgtctca gacggtgat gatgcgcta gaagaagca aacttgagg aaagaagtg atacgtaac aaaatccgg gcgattggt aaaatggct cgagtattc tctgttcta agggaggaa agtgaatca gtgttgcaa cttaaaggt ctcacgcct acgggtgcg ctgcctcta ggcgcgtta tcaggcgga aagacgagt ctcaaaaac gcgttgcaa ggattctct cctaatcat aagataacg agcttcgcg gaagccaaa ggtttagac gccatcaat gagcgcatg cctccgagg aaagatgcg ccaccgtcg ccggctgaa gaaacgcca caggaccac aatcacacc caatctaat gcacactcg tgagctaca cttaaatag gacatcaca ttggccggc tgcaggttt gtgcattag ttt
