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Gene information
General information
TC002813 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9247405..9249175
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002813
    iBeetle screen iB_07983

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): more than 80%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labrum complete not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum position irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002813 RA
      MHTIFTEHQ NAYYRPGGG YSTAAAAVT ATANPYAYE 
      QYSRYGYAA WHHQQHQLS TAKDMVKPP YSYIALIAM 
      AIQNAPDKK ITLNGIYQF IMERFPYYR ENKQGWQNS 
      IRHNLSLNE CFVKVPRDD KKPGKGSYW TLDPDSYNM 
      FDNGSYLRR RRRFKKKDA LREKEEAIK RQQLDSNVT 
      AKRDEKPLE EAKKLECKP KREPAELTQ CKYNDVKSV 
      IVHQEHVIS EAMAMDSAA AFTVDSLMT TREQHAYGN 
      SGRLHTSGG MYYCPSATP QQYIGDEAA PRYSPWYSP 
      ETSPESGYR DMIFDSSAA PSCQLAGFR HSGVSPSPP 
      NYRTAAAYY QQDCVGQKY
    CDS sequence
    • >TC002813 RA
      atgcatacg attttcacc gaacatcag aatgcctac 
      taccggccc gggggcggc tatagcacc gccgccgcc 
      gcggtgacc gccaccgcg aacccctac gcctacgag 
      cagtactcc cgttatggc tacgccgca tggcaccac 
      caacaacac caattatcc accgccaag gacatggtc 
      aaaccgccg tattcctac atcgcgctc atcgccatg 
      gccatccag aacgccccc gacaagaaa atcaccctc 
      aacggcatc taccagttc ataatggag cgctttccc 
      tactaccgc gagaacaaa caaggctgg cagaactca 
      atccgtcac aacctcagc ctgaacgaa tgtttcgtc 
      aaagtccca cgggacgac aaaaagccg ggaaaaggg 
      agctactgg acccttgac ccagactcc tacaacatg 
      ttcgacaac ggctcgtac ctgcgccgc cggcggcgc 
      ttcaagaag aaggacgcc ctccgcgag aaggaggaa 
      gccatcaag cgccaacag ctcgactcc aacgtcacc 
      gccaagcgc gacgaaaaa ccgctggag gaggcgaaa 
      aagctcgag tgcaagccc aagcgcgag cccgcggag 
      ctcacccag tgcaagtac aacgacgtc aagtcggtc 
      atcgtgcac caggagcac gtcatcagc gaggccatg 
      gcgatggac tcggcggcg gccttcacc gtcgactcg 
      ctgatgacg acgcgggag cagcacgcc tacggcaac 
      tccgggcgc ctgcacacc tccggaggg atgtactac 
      tgccccagt gccactccg cagcagtac atcggggac 
      gaggccgcg ccgaggtac agcccctgg tacagcccg 
      gaaacgtcc ccggagtcc ggctacagg gatatgatt 
      ttcgacagc agtgcggcg cccagctgt caattggcg 
      gggtttagg cactccggg gtctcgcca agtccgcct 
      aattataga acggcggcg gcttactac caacaggac 
      tgtgtggga cagaaatac tga
    mRNA sequence
    • >TC002813 RA
      aactgtttt cactagtaa catcgtgtc tcggcaaga 
      agcaacata ctctgtttt tacttaatc tttgtgtaa 
      actgcccca tgttgtgta tcataatat taagtgaac 
      gcaaacttg ttttcggaa gattgattg gaatgaaaa 
      cacaaaacg cactatact gttgaaata acaagactg 
      ttttcgttt tagaaaact ctcggcctt ttgtcttgt 
      ttgttgttt ttgcgtgta aacatgcat acgattttc 
      accgaacat cagaatgcc tactaccgg cccgggggc 
      ggctatagc accgccgcc gccgcggtg accgccacc 
      gcgaacccc tacgcctac gagcagtac tcccgttat 
      ggctacgcc gcatggcac caccaacaa caccaatta 
      tccaccgcc aaggacatg gtcaaaccg ccgtattcc 
      tacatcgcg ctcatcgcc atggccatc cagaacgcc 
      cccgacaag aaaatcacc ctcaacggc atctaccag 
      ttcataatg gagcgcttt ccctactac cgcgagaac 
      aaacaaggc tggcagaac tcaatccgt cacaacctc 
      agcctgaac gaatgtttc gtcaaagtc ccacgggac 
      gacaaaaag ccgggaaaa gggagctac tggaccctt 
      gacccagac tcctacaac atgttcgac aacggctcg 
      tacctgcgc cgccggcgg cgcttcaag aagaaggac 
      gccctccgc gagaaggag gaagccatc aagcgccaa 
      cagctcgac tccaacgtc accgccaag cgcgacgaa 
      aaaccgctg gaggaggcg aaaaagctc gagtgcaag 
      cccaagcgc gagcccgcg gagctcacc cagtgcaag 
      tacaacgac gtcaagtcg gtcatcgtg caccaggag 
      cacgtcatc agcgaggcc atggcgatg gactcggcg 
      gcggccttc accgtcgac tcgctgatg acgacgcgg 
      gagcagcac gcctacggc aactccggg cgcctgcac 
      acctccgga gggatgtac tactgcccc agtgccact 
      ccgcagcag tacatcggg gacgaggcc gcgccgagg 
      tacagcccc tggtacagc ccggaaacg tccccggag 
      tccggctac agggatatg attttcgac agcagtgcg 
      gcgcccagc tgtcaattg gcggggttt aggcactcc 
      ggggtctcg ccaagtccg cctaattat agaacggcg 
      gcggcttac taccaacag gactgtgtg ggacagaaa 
      tactgaggg tacgcgtat catctcaat atcaagact 
      gatgtttga aggttttgt gcgattccg gcctccaag 
      gaattccag tagtgcatg gtggaaatt atagaggtg 
      taggagagg tttagtgag acgattttt cactttcct 
      acaggtctg taatttttt ttcattcga aatcgtttg 
      gctttgttt cgttccaac ggcagctgg ttttattta 
      tgtgatatg ttgatagtg taatttcgt tttttcgaa 
      aatttatat ttttttatt attaggaat ggtgtttct 
      ttttttgta ttatactca taggttcct ataaagatt 
      ttaaccatt ccccgatga atttgcggc atttatcgg 
      ttatcaact ttatagaaa gtaattacc ttacgtgtc 
      cggtgtaca tatttattt gtaaaataa tgtagatgt 
      attattacc cactgtatg tgttgaaag cacgtatgt 
      atattattt attgacttt aaagaaata aatcacacc 
      attgtgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY