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Gene information
General information
TC002838 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8695186..8698503
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002838
      iBeetle screen iB_07981

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002838 RA
        MADAFAFNA RQYFDFTQE HDELNSDSF FDENVEENG 
        PLGQNLEEN FQCNGEVFE REVSDPESD YFFSPMEDT 
        KTNSSTIYL KSRMRKSLS MNDLRLKKV PNQNGLDRA 
        MDELCLNNH QPAEPRPSS ACSLNSRKT ISKEHINRL 
        AQPKRYTSH DNLARQSSM TLAEAVNKF QAGTPKRFR 
        SKPLNNKAP LGPTIPKTP KFLTNARVR PVTALSKEE 
        QEERELEEA RKFKIKANP VNKKVLRGP MKPPVDKKC 
        PTVAEPFHL TEVRKKPEP DEPDKAPSF KAQPVPKSL 
        HNPFRVIPK GQPPTQPES PNFSKKIAR TTPNKVTKQ 
        EEAPKPCKP TNTHVKPFS FEERDKKLM KKKEELINR 
        YLEEEKKAR EFHARPVPK SILRSRNNS QENISDPGS 
        SKSFKRSTE ELDFGTFKA RPAKVLTMK PFEPKKCED 
        HGIVIKEFQ FATNQRLEK RHEFDEAVR AKEARLEAQ 
        KREIEERLR RQEEEEKLK LRKQAELKA QPIKKYKEV 
        TIKPANKIT NPVSPKFSS RVRNKENIE E
      CDS sequence
      • >TC002838 RA
        atggccgat gcatttgct tttaacgcc cgacagtat 
        ttcgatttc actcaagaa cacgacgaa ctaaacagt 
        gattctttc tttgatgaa aatgtagaa gagaacggc 
        cctttaggc cagaacctg gaagaaaat ttccaatgc 
        aatggagag gttttcgaa agggaggtg tctgatccc 
        gaaagtgac tatttcttc tcccctatg gaggataca 
        aagacgaat agctcgact atttatctc aaaagtcgg 
        atgcggaag tctttgagt atgaatgat ttacgtctt 
        aagaaagtg ccaaatcaa aacggactc gatcgagcc 
        atggatgag ttatgtcta aataaccac caacctgca 
        gagcccagg ccgagttct gcttgttcc ttgaacagc 
        agaaaaaca atctcaaag gaacacatc aatcgatta 
        gctcaacct aaacgttac acctcgcac gataacttg 
        gcccgacaa tcgtccatg actttagca gaggctgtc 
        aataaattc caagctggg acacccaaa cgcttccgc 
        tccaaaccc ctaaacaat aaagcccca ctgggtcca 
        actatacca aaaacgccc aaatttctc acaaacgct 
        cgtgtgcgt ccagtgaca gcactctca aaggaagag 
        caagaagaa agggagctt gaagaagcc agaaagttc 
        aagatcaag gcaaatccg gtgaacaag aaagtccta 
        aggggcccc atgaagccc ccagtcgat aaaaagtgc 
        cccactgtt gcggaaccc ttccatttg actgaagtg 
        aggaagaag cccgagccc gatgagcct gataaagcc 
        ccctcattc aaggcacaa cctgtgcct aaaagtttg 
        cacaatcca ttcagagtc atacccaag ggacagccc 
        ccgacccag cctgagtcc cccaatttt tcgaaaaaa 
        attgccaga accacacca aataaagta acgaagcag 
        gaagaagcg ccaaaacca tgcaaacct acaaacaca 
        catgtcaaa ccgtttagt ttcgaagaa cgcgacaag 
        aaactgatg aagaagaaa gaagaattg attaataga 
        tatctggaa gaagagaag aaagctcgt gagttccac 
        gctaggcca gttccgaaa tctatactg aggtcgagg 
        aacaacagc caggaaaat atcagtgat cctggatcg 
        tcaaaatca tttaaacgc agcactgaa gaattggat 
        tttggcact ttcaaagca cggcccgcc aaagtctta 
        actatgaaa ccttttgag ccgaaaaag tgcgaggat 
        catggaatc gtgatcaaa gagtttcaa tttgcgacc 
        aatcaaaga cttgaaaag cgacacgag tttgacgag 
        gcagtgaga gctaaggaa gcgagactt gaagctcaa 
        aaacgggaa attgaggag agactgagg cgacaggaa 
        gaagaggag aagttgaag ttgagaaaa caggcggag 
        ttaaaagcg caaccaatt aaaaagtac aaggaagta 
        accatcaag ccggcgaat aaaattaca aacccggtc 
        agtccgaag ttcagttcc agggtcagg aacaaggag 
        aatattgag gaataa
      mRNA sequence
      • >TC002838 RA
        agactaaaa tatatattt tagtttctg tctataggg 
        actaactaa cggttgcac agatcagtt catctcaaa 
        ctttgtttt tgtggcctg aaagccggt aattatttt 
        gctaaaatc atggccgat gcatttgct tttaacgcc 
        cgacagtat ttcgatttc actcaagaa cacgacgaa 
        ctaaacagt gattctttc tttgatgaa aatgtagaa 
        gagaacggc cctttaggc cagaacctg gaagaaaat 
        ttccaatgc aatggagag gttttcgaa agggaggtg 
        tctgatccc gaaagtgac tatttcttc tcccctatg 
        gaggataca aagacgaat agctcgact atttatctc 
        aaaagtcgg atgcggaag tctttgagt atgaatgat 
        ttacgtctt aagaaagtg ccaaatcaa aacggactc 
        gatcgagcc atggatgag ttatgtcta aataaccac 
        caacctgca gagcccagg ccgagttct gcttgttcc 
        ttgaacagc agaaaaaca atctcaaag gaacacatc 
        aatcgatta gctcaacct aaacgttac acctcgcac 
        gataacttg gcccgacaa tcgtccatg actttagca 
        gaggctgtc aataaattc caagctggg acacccaaa 
        cgcttccgc tccaaaccc ctaaacaat aaagcccca 
        ctgggtcca actatacca aaaacgccc aaatttctc 
        acaaacgct cgtgtgcgt ccagtgaca gcactctca 
        aaggaagag caagaagaa agggagctt gaagaagcc 
        agaaagttc aagatcaag gcaaatccg gtgaacaag 
        aaagtccta aggggcccc atgaagccc ccagtcgat 
        aaaaagtgc cccactgtt gcggaaccc ttccatttg 
        actgaagtg aggaagaag cccgagccc gatgagcct 
        gataaagcc ccctcattc aaggcacaa cctgtgcct 
        aaaagtttg cacaatcca ttcagagtc atacccaag 
        ggacagccc ccgacccag cctgagtcc cccaatttt 
        tcgaaaaaa attgccaga accacacca aataaagta 
        acgaagcag gaagaagcg ccaaaacca tgcaaacct 
        acaaacaca catgtcaaa ccgtttagt ttcgaagaa 
        cgcgacaag aaactgatg aagaagaaa gaagaattg 
        attaataga tatctggaa gaagagaag aaagctcgt 
        gagttccac gctaggcca gttccgaaa tctatactg 
        aggtcgagg aacaacagc caggaaaat atcagtgat 
        cctggatcg tcaaaatca tttaaacgc agcactgaa 
        gaattggat tttggcact ttcaaagca cggcccgcc 
        aaagtctta actatgaaa ccttttgag ccgaaaaag 
        tgcgaggat catggaatc gtgatcaaa gagtttcaa 
        tttgcgacc aatcaaaga cttgaaaag cgacacgag 
        tttgacgag gcagtgaga gctaaggaa gcgagactt 
        gaagctcaa aaacgggaa attgaggag agactgagg 
        cgacaggaa gaagaggag aagttgaag ttgagaaaa 
        caggcggag ttaaaagcg caaccaatt aaaaagtac 
        aaggaagta accatcaag ccggcgaat aaaattaca 
        aacccggtc agtccgaag ttcagttcc agggtcagg 
        aacaaggag aatattgag gaataattt gtaattgtt 
        taaggtgat attattttt acgtt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY