General information | |||
TC002912 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 6596634..6599083 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC002912 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07975 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)previtellogenic egg chamber - Number of animals showing the phenotype: 2
previtellogenic egg chamber not present - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002912 RA
MDAYDIFKK LSQGAKFSR EHNIKKSYS NNDVIKVEK NDNEPKNQP VSIKKEKEE NPITLLSSL KSSTETRGK KRKHSDPES EEKKKKLLK QEEVNHYRN LNKISVVGK RVPEPVKTF DDLNVDPII INNVKKCGY IEPTPVQKQ AVPIMLEGR NLLACAPTG SGKTAAFLI PILHDLKGP RKEGFRALV LCPTRELAK QTQRECIRL SEGKGFHVH VISKINKAL IQYGPNSSQ KFDILITTP NRVCFLLKQ DQAALSLAN IKWLVIDEA DKLFETGNR GFREQLDQI LNACTNKEK KVAMFSATY TPMVAKWCV HNMKGLVRI TVGQRNAAA DTVDQELLF VGNEQGKLL AFRDLVKKG LTPPVLVFV QSKDRAQQL FNELIYDGI NVDAIHADR TQLQRDNTV RSFREGRIW VLICTELMA RGIDFKGVN LVINYDFPP SAISYVHRV GRAGRAGRT GKAITFFTV ENTVNLRSI AYVLKESGC EVPSYMLTM KKTSKKDRK KLEKAAPKR DDIDTRPKY EILKRRLKK KKGTPINRE GAKTQKSDN KKKTKSKKT KENTESKTS KTVVKKGKK KNALTK
- >TC002912 RA
atggatgct tatgatatt tttaaaaaa ttaagtcag ggtgcgaag ttttctcgt gaacataac atcaagaaa agttatagc aacaatgat gtgataaaa gttgagaaa aatgataac gaaccaaaa aatcaacct gtatctatc aaaaaggaa aaggaggaa aatcctatc actctttta agtagttta aaatcttca acagagact agaggcaag aaacgtaag cattccgac cctgaatct gaagaaaaa aagaaaaag ttattaaaa caggaagaa gtgaaccat tacagaaat ttaaacaaa atatctgtt gttggcaaa cgcgtacca gaacctgtt aaaacattt gacgattta aacgtggac cctatcata atcaataat gtaaaaaaa tgcggttac attgaacca actcctgtg caaaaacaa gcagttcca attatgcta gaggggaga aatttacta gcgtgtgcc cctactggg tctggcaaa actgcagcg tttttgatc ccgatttta cacgatctt aaagggccc agaaaagag ggttttagg gctctggtt ttgtgccca accagagag ttggcaaag cagacgcaa agggaatgt attcgttta tcagaagga aagggtttt cacgtgcat gtgataagc aaaataaat aaagccctg atacagtat ggaccaaac agttcgcaa aaatttgat attttgatt acaactccg aacagggtt tgcttttta ttaaagcaa gatcaggct gcattatca ttagcaaat atcaaatgg ttagtcatt gatgaggca gataagctt tttgaaact ggaaatagg ggctttaga gagcagttg gaccaaata ttaaatgct tgtacaaac aaagagaaa aaagtggcc atgtttagt gccacatac acaccaatg gttgctaaa tggtgtgtt cataatatg aaaggatta gttcgcatt actgttgga caaagaaat gctgcagca gatactgtt gaccaagaa ttattattt gttggtaac gaacaggga aaattatta gcttttaga gatttggtc aaaaaaggt ttgacacct ccagttttg gttttcgtt caaagtaaa gacagagcg caacagttg tttaatgaa ttgatttat gatggaatt aacgttgat gctatccat gcagacaga acacaactg cagagagat aataccgtt agaagtttt cgggaaggt cgtatttgg gtgttaatt tgcactgag ttgatggct cgcggtatt gattttaaa ggagttaat ttagtgata aactatgat tttccaccg tcggctatt tcttacgtt catagggtg ggaagggct ggacgtgcg ggacgtaca ggcaaagct attactttt ttcacagtt gagaatact gttaattta agaagtatt gcttatgtt ttgaaagaa tctggttgt gaagttccc tcatatatg cttactatg aagaaaact tcgaaaaaa gataggaaa aaattggag aaagcggcg cctaaaaga gatgatatt gatacgagg cctaaatat gaaattttg aagaggcgc ttgaagaaa aagaaagga acaccaata aatcgagag ggtgcaaag acccaaaaa tcagataat aagaaaaaa acgaaaagc aaaaaaacc aaagaaaac acagaatcc aaaacgagt aaaacggta gtgaaaaaa ggaaaaaag aaaaatgct ttgactaaa taa
- >TC002912 RA
tggtaggta acctacaaa acgtgttaa gataaccta aaaaaaaaa taagttcaa tttaattaa agggggtta gttaataaa acctaataa tacaaacca atgtagaaa ggtccaaaa aatggatgc ttatgatat ttttaaaaa attaagtca gggtgcgaa gttttctcg tgaacataa catcaagaa aagttatag caacaatga tgtgataaa agttgagaa aaatgataa cgaaccaaa aaatcaacc tgtatctat caaaaagga aaaggagga aaatcctat cactctttt aagtagttt aaaatcttc aacagagac tagaggcaa gaaacgtaa gcattccga ccctgaatc tgaagaaaa aaagaaaaa gttattaaa acaggaaga agtgaacca ttacagaaa tttaaacaa aatatctgt tgttggcaa acgcgtacc agaacctgt taaaacatt tgacgattt aaacgtgga ccctatcat aatcaataa tgtaaaaaa atgcggtta cattgaacc aactcctgt gcaaaaaca agcagttcc aattatgct agaggggag aaatttact agcgtgtgc ccctactgg gtctggcaa aactgcagc gtttttgat cccgatttt acacgatct taaagggcc cagaaaaga gggttttag ggctctggt tttgtgccc aaccagaga gttggcaaa gcagacgca aagggaatg tattcgttt atcagaagg aaagggttt tcacgtgca tgtgataag caaaataaa taaagccct gatacagta tggaccaaa cagttcgca aaaatttga tattttgat tacaactcc gaacagggt ttgcttttt attaaagca agatcaggc tgcattatc attagcaaa tatcaaatg gttagtcat tgatgaggc agataagct ttttgaaac tggaaatag gggctttag agagcagtt ggaccaaat attaaatgc ttgtacaaa caaagagaa aaaagtggc catgtttag tgccacata cacaccaat ggttgctaa atggtgtgt tcataatat gaaaggatt agttcgcat tactgttgg acaaagaaa tgctgcagc agatactgt tgaccaaga attattatt tgttggtaa cgaacaggg aaaattatt agcttttag agatttggt caaaaaagg tttgacacc tccagtttt ggttttcgt tcaaagtaa agacagagc gcaacagtt gtttaatga attgattta tgatggaat taacgttga tgctatcca tgcagacag aacacaact gcagagaga taataccgt tagaagttt tcgggaagg tcgtatttg ggtgttaat ttgcactga gttgatggc tcgcggtat tgattttaa aggagttaa tttagtgat aaactatga ttttccacc gtcggctat ttcttacgt tcatagggt gggaagggc tggacgtgc gggacgtac aggcaaagc tattacttt tttcacagt tgagaatac tgttaattt aagaagtat tgcttatgt tttgaaaga atctggttg tgaagttcc ctcatatat gcttactat gaagaaaac ttcgaaaaa agataggaa aaaattgga gaaagcggc gcctaaaag agatgatat tgatacgag gcctaaata tgaaatttt gaagaggcg cttgaagaa aaagaaagg aacaccaat aaatcgaga gggtgcaaa gacccaaaa atcagataa taagaaaaa aacgaaaag caaaaaaac caaagaaaa cacagaatc caaaacgag taaaacggt agtgaaaaa aggaaaaaa gaaaaatgc tttgactaa ataataaat taataaata actttaatg caa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
