Status
Please wait ...
Gene information
General information
TC002912 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6596634..6599083
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002912
    iBeetle screen iB_07975

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    previtellogenic egg chamber - Number of animals showing the phenotype: 2

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002912 RA
      MDAYDIFKK LSQGAKFSR EHNIKKSYS NNDVIKVEK 
NDNEPKNQP VSIKKEKEE NPITLLSSL KSSTETRGK 
KRKHSDPES EEKKKKLLK QEEVNHYRN LNKISVVGK 
RVPEPVKTF DDLNVDPII INNVKKCGY IEPTPVQKQ 
AVPIMLEGR NLLACAPTG SGKTAAFLI PILHDLKGP 
RKEGFRALV LCPTRELAK QTQRECIRL SEGKGFHVH 
VISKINKAL IQYGPNSSQ KFDILITTP NRVCFLLKQ 
DQAALSLAN IKWLVIDEA DKLFETGNR GFREQLDQI 
LNACTNKEK KVAMFSATY TPMVAKWCV HNMKGLVRI 
TVGQRNAAA DTVDQELLF VGNEQGKLL AFRDLVKKG 
LTPPVLVFV QSKDRAQQL FNELIYDGI NVDAIHADR 
TQLQRDNTV RSFREGRIW VLICTELMA RGIDFKGVN 
LVINYDFPP SAISYVHRV GRAGRAGRT GKAITFFTV 
ENTVNLRSI AYVLKESGC EVPSYMLTM KKTSKKDRK 
KLEKAAPKR DDIDTRPKY EILKRRLKK KKGTPINRE 
GAKTQKSDN KKKTKSKKT KENTESKTS KTVVKKGKK 
KNALTK
    CDS sequence
    • >TC002912 RA
      atggatgct tatgatatt tttaaaaaa ttaagtcag 
ggtgcgaag ttttctcgt gaacataac atcaagaaa 
agttatagc aacaatgat gtgataaaa gttgagaaa 
aatgataac gaaccaaaa aatcaacct gtatctatc 
aaaaaggaa aaggaggaa aatcctatc actctttta 
agtagttta aaatcttca acagagact agaggcaag 
aaacgtaag cattccgac cctgaatct gaagaaaaa 
aagaaaaag ttattaaaa caggaagaa gtgaaccat 
tacagaaat ttaaacaaa atatctgtt gttggcaaa 
cgcgtacca gaacctgtt aaaacattt gacgattta 
aacgtggac cctatcata atcaataat gtaaaaaaa 
tgcggttac attgaacca actcctgtg caaaaacaa 
gcagttcca attatgcta gaggggaga aatttacta 
gcgtgtgcc cctactggg tctggcaaa actgcagcg 
tttttgatc ccgatttta cacgatctt aaagggccc 
agaaaagag ggttttagg gctctggtt ttgtgccca 
accagagag ttggcaaag cagacgcaa agggaatgt 
attcgttta tcagaagga aagggtttt cacgtgcat 
gtgataagc aaaataaat aaagccctg atacagtat 
ggaccaaac agttcgcaa aaatttgat attttgatt 
acaactccg aacagggtt tgcttttta ttaaagcaa 
gatcaggct gcattatca ttagcaaat atcaaatgg 
ttagtcatt gatgaggca gataagctt tttgaaact 
ggaaatagg ggctttaga gagcagttg gaccaaata 
ttaaatgct tgtacaaac aaagagaaa aaagtggcc 
atgtttagt gccacatac acaccaatg gttgctaaa 
tggtgtgtt cataatatg aaaggatta gttcgcatt 
actgttgga caaagaaat gctgcagca gatactgtt 
gaccaagaa ttattattt gttggtaac gaacaggga 
aaattatta gcttttaga gatttggtc aaaaaaggt 
ttgacacct ccagttttg gttttcgtt caaagtaaa 
gacagagcg caacagttg tttaatgaa ttgatttat 
gatggaatt aacgttgat gctatccat gcagacaga 
acacaactg cagagagat aataccgtt agaagtttt 
cgggaaggt cgtatttgg gtgttaatt tgcactgag 
ttgatggct cgcggtatt gattttaaa ggagttaat 
ttagtgata aactatgat tttccaccg tcggctatt 
tcttacgtt catagggtg ggaagggct ggacgtgcg 
ggacgtaca ggcaaagct attactttt ttcacagtt 
gagaatact gttaattta agaagtatt gcttatgtt 
ttgaaagaa tctggttgt gaagttccc tcatatatg 
cttactatg aagaaaact tcgaaaaaa gataggaaa 
aaattggag aaagcggcg cctaaaaga gatgatatt 
gatacgagg cctaaatat gaaattttg aagaggcgc 
ttgaagaaa aagaaagga acaccaata aatcgagag 
ggtgcaaag acccaaaaa tcagataat aagaaaaaa 
acgaaaagc aaaaaaacc aaagaaaac acagaatcc 
aaaacgagt aaaacggta gtgaaaaaa ggaaaaaag 
aaaaatgct ttgactaaa taa
    mRNA sequence
    • >TC002912 RA
      tggtaggta acctacaaa acgtgttaa gataaccta 
aaaaaaaaa taagttcaa tttaattaa agggggtta 
gttaataaa acctaataa tacaaacca atgtagaaa 
ggtccaaaa aatggatgc ttatgatat ttttaaaaa 
attaagtca gggtgcgaa gttttctcg tgaacataa 
catcaagaa aagttatag caacaatga tgtgataaa 
agttgagaa aaatgataa cgaaccaaa aaatcaacc 
tgtatctat caaaaagga aaaggagga aaatcctat 
cactctttt aagtagttt aaaatcttc aacagagac 
tagaggcaa gaaacgtaa gcattccga ccctgaatc 
tgaagaaaa aaagaaaaa gttattaaa acaggaaga 
agtgaacca ttacagaaa tttaaacaa aatatctgt 
tgttggcaa acgcgtacc agaacctgt taaaacatt 
tgacgattt aaacgtgga ccctatcat aatcaataa 
tgtaaaaaa atgcggtta cattgaacc aactcctgt 
gcaaaaaca agcagttcc aattatgct agaggggag 
aaatttact agcgtgtgc ccctactgg gtctggcaa 
aactgcagc gtttttgat cccgatttt acacgatct 
taaagggcc cagaaaaga gggttttag ggctctggt 
tttgtgccc aaccagaga gttggcaaa gcagacgca 
aagggaatg tattcgttt atcagaagg aaagggttt 
tcacgtgca tgtgataag caaaataaa taaagccct 
gatacagta tggaccaaa cagttcgca aaaatttga 
tattttgat tacaactcc gaacagggt ttgcttttt 
attaaagca agatcaggc tgcattatc attagcaaa 
tatcaaatg gttagtcat tgatgaggc agataagct 
ttttgaaac tggaaatag gggctttag agagcagtt 
ggaccaaat attaaatgc ttgtacaaa caaagagaa 
aaaagtggc catgtttag tgccacata cacaccaat 
ggttgctaa atggtgtgt tcataatat gaaaggatt 
agttcgcat tactgttgg acaaagaaa tgctgcagc 
agatactgt tgaccaaga attattatt tgttggtaa 
cgaacaggg aaaattatt agcttttag agatttggt 
caaaaaagg tttgacacc tccagtttt ggttttcgt 
tcaaagtaa agacagagc gcaacagtt gtttaatga 
attgattta tgatggaat taacgttga tgctatcca 
tgcagacag aacacaact gcagagaga taataccgt 
tagaagttt tcgggaagg tcgtatttg ggtgttaat 
ttgcactga gttgatggc tcgcggtat tgattttaa 
aggagttaa tttagtgat aaactatga ttttccacc 
gtcggctat ttcttacgt tcatagggt gggaagggc 
tggacgtgc gggacgtac aggcaaagc tattacttt 
tttcacagt tgagaatac tgttaattt aagaagtat 
tgcttatgt tttgaaaga atctggttg tgaagttcc 
ctcatatat gcttactat gaagaaaac ttcgaaaaa 
agataggaa aaaattgga gaaagcggc gcctaaaag 
agatgatat tgatacgag gcctaaata tgaaatttt 
gaagaggcg cttgaagaa aaagaaagg aacaccaat 
aaatcgaga gggtgcaaa gacccaaaa atcagataa 
taagaaaaa aacgaaaag caaaaaaac caaagaaaa 
cacagaatc caaaacgag taaaacggt agtgaaaaa 
aggaaaaaa gaaaaatgc tttgactaa ataataaat 
taataaata actttaatg caa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY