Status
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Gene information
General information
TC002912 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6596634..6599083
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002912
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07975

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    previtellogenic egg chamber - Number of animals showing the phenotype: 2

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002912 RA
      MDAYDIFKK LSQGAKFSR EHNIKKSYS NNDVIKVEK 
      NDNEPKNQP VSIKKEKEE NPITLLSSL KSSTETRGK 
      KRKHSDPES EEKKKKLLK QEEVNHYRN LNKISVVGK 
      RVPEPVKTF DDLNVDPII INNVKKCGY IEPTPVQKQ 
      AVPIMLEGR NLLACAPTG SGKTAAFLI PILHDLKGP 
      RKEGFRALV LCPTRELAK QTQRECIRL SEGKGFHVH 
      VISKINKAL IQYGPNSSQ KFDILITTP NRVCFLLKQ 
      DQAALSLAN IKWLVIDEA DKLFETGNR GFREQLDQI 
      LNACTNKEK KVAMFSATY TPMVAKWCV HNMKGLVRI 
      TVGQRNAAA DTVDQELLF VGNEQGKLL AFRDLVKKG 
      LTPPVLVFV QSKDRAQQL FNELIYDGI NVDAIHADR 
      TQLQRDNTV RSFREGRIW VLICTELMA RGIDFKGVN 
      LVINYDFPP SAISYVHRV GRAGRAGRT GKAITFFTV 
      ENTVNLRSI AYVLKESGC EVPSYMLTM KKTSKKDRK 
      KLEKAAPKR DDIDTRPKY EILKRRLKK KKGTPINRE 
      GAKTQKSDN KKKTKSKKT KENTESKTS KTVVKKGKK 
      KNALTK
    CDS sequence
    • >TC002912 RA
      atggatgct tatgatatt tttaaaaaa ttaagtcag 
      ggtgcgaag ttttctcgt gaacataac atcaagaaa 
      agttatagc aacaatgat gtgataaaa gttgagaaa 
      aatgataac gaaccaaaa aatcaacct gtatctatc 
      aaaaaggaa aaggaggaa aatcctatc actctttta 
      agtagttta aaatcttca acagagact agaggcaag 
      aaacgtaag cattccgac cctgaatct gaagaaaaa 
      aagaaaaag ttattaaaa caggaagaa gtgaaccat 
      tacagaaat ttaaacaaa atatctgtt gttggcaaa 
      cgcgtacca gaacctgtt aaaacattt gacgattta 
      aacgtggac cctatcata atcaataat gtaaaaaaa 
      tgcggttac attgaacca actcctgtg caaaaacaa 
      gcagttcca attatgcta gaggggaga aatttacta 
      gcgtgtgcc cctactggg tctggcaaa actgcagcg 
      tttttgatc ccgatttta cacgatctt aaagggccc 
      agaaaagag ggttttagg gctctggtt ttgtgccca 
      accagagag ttggcaaag cagacgcaa agggaatgt 
      attcgttta tcagaagga aagggtttt cacgtgcat 
      gtgataagc aaaataaat aaagccctg atacagtat 
      ggaccaaac agttcgcaa aaatttgat attttgatt 
      acaactccg aacagggtt tgcttttta ttaaagcaa 
      gatcaggct gcattatca ttagcaaat atcaaatgg 
      ttagtcatt gatgaggca gataagctt tttgaaact 
      ggaaatagg ggctttaga gagcagttg gaccaaata 
      ttaaatgct tgtacaaac aaagagaaa aaagtggcc 
      atgtttagt gccacatac acaccaatg gttgctaaa 
      tggtgtgtt cataatatg aaaggatta gttcgcatt 
      actgttgga caaagaaat gctgcagca gatactgtt 
      gaccaagaa ttattattt gttggtaac gaacaggga 
      aaattatta gcttttaga gatttggtc aaaaaaggt 
      ttgacacct ccagttttg gttttcgtt caaagtaaa 
      gacagagcg caacagttg tttaatgaa ttgatttat 
      gatggaatt aacgttgat gctatccat gcagacaga 
      acacaactg cagagagat aataccgtt agaagtttt 
      cgggaaggt cgtatttgg gtgttaatt tgcactgag 
      ttgatggct cgcggtatt gattttaaa ggagttaat 
      ttagtgata aactatgat tttccaccg tcggctatt 
      tcttacgtt catagggtg ggaagggct ggacgtgcg 
      ggacgtaca ggcaaagct attactttt ttcacagtt 
      gagaatact gttaattta agaagtatt gcttatgtt 
      ttgaaagaa tctggttgt gaagttccc tcatatatg 
      cttactatg aagaaaact tcgaaaaaa gataggaaa 
      aaattggag aaagcggcg cctaaaaga gatgatatt 
      gatacgagg cctaaatat gaaattttg aagaggcgc 
      ttgaagaaa aagaaagga acaccaata aatcgagag 
      ggtgcaaag acccaaaaa tcagataat aagaaaaaa 
      acgaaaagc aaaaaaacc aaagaaaac acagaatcc 
      aaaacgagt aaaacggta gtgaaaaaa ggaaaaaag 
      aaaaatgct ttgactaaa taa
    mRNA sequence
    • >TC002912 RA
      tggtaggta acctacaaa acgtgttaa gataaccta 
      aaaaaaaaa taagttcaa tttaattaa agggggtta 
      gttaataaa acctaataa tacaaacca atgtagaaa 
      ggtccaaaa aatggatgc ttatgatat ttttaaaaa 
      attaagtca gggtgcgaa gttttctcg tgaacataa 
      catcaagaa aagttatag caacaatga tgtgataaa 
      agttgagaa aaatgataa cgaaccaaa aaatcaacc 
      tgtatctat caaaaagga aaaggagga aaatcctat 
      cactctttt aagtagttt aaaatcttc aacagagac 
      tagaggcaa gaaacgtaa gcattccga ccctgaatc 
      tgaagaaaa aaagaaaaa gttattaaa acaggaaga 
      agtgaacca ttacagaaa tttaaacaa aatatctgt 
      tgttggcaa acgcgtacc agaacctgt taaaacatt 
      tgacgattt aaacgtgga ccctatcat aatcaataa 
      tgtaaaaaa atgcggtta cattgaacc aactcctgt 
      gcaaaaaca agcagttcc aattatgct agaggggag 
      aaatttact agcgtgtgc ccctactgg gtctggcaa 
      aactgcagc gtttttgat cccgatttt acacgatct 
      taaagggcc cagaaaaga gggttttag ggctctggt 
      tttgtgccc aaccagaga gttggcaaa gcagacgca 
      aagggaatg tattcgttt atcagaagg aaagggttt 
      tcacgtgca tgtgataag caaaataaa taaagccct 
      gatacagta tggaccaaa cagttcgca aaaatttga 
      tattttgat tacaactcc gaacagggt ttgcttttt 
      attaaagca agatcaggc tgcattatc attagcaaa 
      tatcaaatg gttagtcat tgatgaggc agataagct 
      ttttgaaac tggaaatag gggctttag agagcagtt 
      ggaccaaat attaaatgc ttgtacaaa caaagagaa 
      aaaagtggc catgtttag tgccacata cacaccaat 
      ggttgctaa atggtgtgt tcataatat gaaaggatt 
      agttcgcat tactgttgg acaaagaaa tgctgcagc 
      agatactgt tgaccaaga attattatt tgttggtaa 
      cgaacaggg aaaattatt agcttttag agatttggt 
      caaaaaagg tttgacacc tccagtttt ggttttcgt 
      tcaaagtaa agacagagc gcaacagtt gtttaatga 
      attgattta tgatggaat taacgttga tgctatcca 
      tgcagacag aacacaact gcagagaga taataccgt 
      tagaagttt tcgggaagg tcgtatttg ggtgttaat 
      ttgcactga gttgatggc tcgcggtat tgattttaa 
      aggagttaa tttagtgat aaactatga ttttccacc 
      gtcggctat ttcttacgt tcatagggt gggaagggc 
      tggacgtgc gggacgtac aggcaaagc tattacttt 
      tttcacagt tgagaatac tgttaattt aagaagtat 
      tgcttatgt tttgaaaga atctggttg tgaagttcc 
      ctcatatat gcttactat gaagaaaac ttcgaaaaa 
      agataggaa aaaattgga gaaagcggc gcctaaaag 
      agatgatat tgatacgag gcctaaata tgaaatttt 
      gaagaggcg cttgaagaa aaagaaagg aacaccaat 
      aaatcgaga gggtgcaaa gacccaaaa atcagataa 
      taagaaaaa aacgaaaag caaaaaaac caaagaaaa 
      cacagaatc caaaacgag taaaacggt agtgaaaaa 
      aggaaaaaa gaaaaatgc tttgactaa ataataaat 
      taataaata actttaatg caa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY