Status
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Gene information
General information
TC002957 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5820132..5827882
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002957
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07970

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002957 RA
      MFPTFISIL DIQSWWEVP SIAHFCSLF RTAFNLLDF 
      DIEDLEEAL LTDGTEETS WLQELIVRL LSGCLPNNE 
      ISTFNYQMF LRRLFRQKC QEHDQYNPF NTDVDFQLL 
      PLRTKVDIL HALCDFRLD ADDVLDQLK NLEADSLRV 
      EPLGYDSNE SAYWYFYGT RLYREDFKT KNNKKKSVW 
      QVICFTEDD WFQLNKKFK KSTAKCERE LHRTLSENF 
      LPELPRLFR EKERLARKR LLENQPRRT SRRLRKENT 
      TEKEEVTCE SPKTNPLEE EERQRKRDL EIKRKEAYE 
      RSNRLKEQS EDSQSEDSE VRYEMGGTK TRQKKSKGR 
      QRKLSTSSS NSKDASSAP KSSKRKKTG EETSKSSTS 
      SATTTVGRQ TNNSLAAAT GQIVIQPVT NSHKKKLKT 
      SQVFRQTDE DLITGMHKI LDYVKNHED AWPFADPVD 
      EDYAPNYYT VIRKPMDLQ RMEERLDAG YYKNFAKFR 
      DDFQLIVDN CRLYNGVEN EYTEMVDNL LKVFERATE 
      KFLDQISSS DDEIAVEFP NTDLDAKDT KKNNKPESK 
      SSKRKPTLA AKTDKKREH SPSEESLGK RSLSCDSLI 
      EEDEKISSN KKDKNTKKT NKVAKKDKN DTKKNTRDK 
      KNSNSKHKK EKFSSPLRS RSPSRSVSR DSFRRSYSS 
      SPPPSSVPS SPDNRFDFF PKKHKSFED KDSDTHSGP 
      KGKQKQKQD DTNTCDTSA KKQNDKPVT SPSKPKDKH 
      NKLRETIEK LKAKSEISN KLNDDEFRF DLDTFKENK 
      EKSKKDDKS RKKKDKVTK PHLKKDLDF EDSNSTMES 
      LHTTDNKKK KTNTKNCKN ASIDALTIA TEQTLKDIN 
      KWLDDTPKY SEFSSASNS PCYPPLDDF DIVNKIDND 
      PRKKVEKFN SKKDGIGKD IKRRTFSRD PAKFLKRRE 
      VQRTIDRLQ PGKSKGNLI SSVQSSKQT DELFPLGPL 
      SKNKDTKNS LIVKTDDNA PKLSLGSVL DSFGRHKFV 
      DDTKQDESE NATKKDEKF SISTEIKEE TQIKPKLED 
      VKKESPVRE ESSMMSGAT PNLSAWFKA FGAPKIQSS 
      QKKSEKSEG KESEKTDDS KVDPKSKEE AKVPVPEHS 
      PSTDSPVAT HGQPVPRQR KVSTGSSIS ERSSFSQDM 
      DSPRVGIDE RLGAYPAPY PSPMHRSPS GASPVMASP 
      RPDVSPKAA PYPPFNGQI RVGFYQDTV SNKSSPDKS 
      CSPRDNPQS PYAQCSEHV YTPNTTQNM PYTYPNSPY 
      YSHTPNYSN TNPTPPYNP DSVNPPSYY DTNKSLTDQ 
      YQAKNIQNY VNSPNNVQS PSPHPPIET FRQEDQNQN 
      KVNEKMVQS AMFPVKKRA YNESDSAQM SHRYEEVNR 
      NIQPMEGQN TQNVDTMHM NAREESMKH YIHQNIPDP 
      KPLTVQTNV SPQQQLASP IINYSTPSN PYNNYSNES 
      NYPLNILSR SLASTPGIE RKLDIGKYT NMGYSGPEV 
      NFPRGMQQY SRSELSYAR PPSNDQQPV SNQGQQPSE 
      VLHYSNASP HMGYKNVDM NAATSNPQQ AHTNYTNTS 
      VHSNPNRLG QPLDVQSSY KSNEVSIPR PTYNTHIDM 
      DQPLGLRGN IANLSHIVD RYNNDERML TGLQPAASQ 
      YYGDKGLGG PHMFNKPIC TSSSGLPIF NQPNMIQSY 
      NQNMQVPTS SMYNRQMTE LQNPMSGEA KNANVAQQG 
      SEKKSKKRK SAKTAQAAS ASEPPAQAN NQGFQSYAG 
      LKSTSPMEP SAISLKTAS VVPGSAFNF GPSPSALGI 
      GSGLYGDKD AYPNFLEDF RAAPNYYIA AATAAHHRS 
      TPDAPDKQT RVAAHQSSA RQAPSYPFI GARQATPPS 
      YPLSSHFMP SAQAPLMDA SSPLYQHYL QAGVLNQGL 
      LGPPGPYPP GYHPALSMR QPYDSMTRP PWL
    CDS sequence
    • >TC002957 RA
      atgtttcca actttcatt agcatccta gatattcaa 
      tcatggtgg gaggttcca agcattgca catttttgt 
      tcattgttt cgaacagca tttaacctt ttggatttt 
      gatatcgag gatttggag gaggctctc ttgactgat 
      gggacggag gaaacctct tggcttcaa gaactgatc 
      gtgaggctt ctcagtggg tgcttgccg aacaatgaa 
      atctcaacg ttcaattat cagatgttc ctgagacgt 
      ctctttcgc cagaaatgt caggaacat gatcaatac 
      aacccattt aacacagac gttgatttc caattgtta 
      cctttgaga acaaaagtg gacatttta catgcactc 
      tgtgatttt cgtttagat gctgatgat gtgcttgac 
      cagctgaaa aatttagaa gcagacagc ttgagggtt 
      gagcccctg ggttatgac agtaacgaa tctgcttac 
      tggtatttt tacggtact agactgtac agagaggac 
      tttaaaacc aagaataat aagaagaaa tccgtgtgg 
      caggttatt tgtttcact gaagacgat tggtttcaa 
      cttaacaag aaatttaaa aaatcgact gctaagtgt 
      gaaagagag ttacatcgt actttaagt gagaacttc 
      ttgcctgaa ttgcccaga ttgttccgg gaaaaggaa 
      agactggcc aggaagcgt ctgctggaa aatcaaccg 
      agacgcact tctcgacgt ttgagaaaa gaaaataca 
      actgaaaaa gaagaagta acgtgtgaa tcgccgaaa 
      accaatcct cttgaagag gaagaaaga caaagaaag 
      cgcgatttg gaaatcaag agaaaagaa gcttatgag 
      agaagtaac agacttaaa gagcagagc gaagatagc 
      caaagtgag gactctgaa gtacggtat gagatgggc 
      gggactaaa acgcgacaa aagaagtcc aaaggaaga 
      caaagaaaa ttgtcaaca agttcctct aacagtaaa 
      gatgcatca tctgcgccg aagagctca aaaaggaag 
      aaaacaggt gaggagact tcaaaatcc tcgacttct 
      tccgcaact acaacagtt ggtcgacaa accaacaat 
      tcgctggcg gcagctact ggacaaatt gttatccaa 
      cctgttacc aactcacac aagaaaaaa ctaaaaact 
      tctcaagtg tttcgtcaa accgacgaa gatctaata 
      acaggaatg cacaagatc ttagattac gtaaaaaat 
      catgaagac gcatggccc tttgccgat cctgttgat 
      gaggattac gcacccaat tattacaca gttattcgg 
      aaaccaatg gatttgcaa agaatggaa gagaggctg 
      gatgcaggt tattacaag aactttgca aaattcaga 
      gacgatttc cagttaatc gtggacaat tgtcgactt 
      tataacggt gttgaaaat gaatatacg gaaatggtc 
      gataatttg ttgaaagta tttgaaaga gcaactgag 
      aaatttttg gaccaaatc tcatcatcc gacgacgaa 
      atcgccgtt gaattccct aatactgat ctggacgca 
      aaagatacc aaaaagaac aacaaacca gaatctaaa 
      tcatcaaag cgtaaaccg actcttgca gctaaaaca 
      gataaaaag agggagcat tcgccctct gaagaatct 
      ctaggtaaa aggagtctt tcctgtgat tcgcttatt 
      gaggaagat gaaaagata tcgtcgaat aaaaaagac 
      aaaaacact aagaaaaca aataaagta gctaaaaag 
      gataaaaac gacactaag aaaaacaca cgagataag 
      aagaattct aatagtaaa cacaagaaa gagaaattt 
      tcaagtccc ttgagaagt agaagtcct agcaggagc 
      gttagtcga gattccttt aggcgaagt tatagcagt 
      agtcctccg ccctcaagt gtcccttcc agtcccgat 
      aacaggttc gattttttc ccaaaaaaa cataagtct 
      tttgaagat aaggactcg gacacccat tcagggcct 
      aaaggtaaa cagaagcag aagcaagac gacacaaac 
      acatgtgat acttcagca aagaaacaa aatgacaaa 
      ccggtcaca tcgccatca aaaccgaaa gataaacat 
      aacaagctt cgagaaact atagaaaaa ttaaaagcc 
      aaaagtgaa atttcgaat aaattgaac gacgacgag 
      ttccgtttc gacttggat acctttaaa gaaaacaag 
      gaaaagtcc aagaaagac gacaaaagt cgaaagaag 
      aaggataaa gtgactaag ccacacttg aagaaagac 
      ctcgatttt gaagatagt aatagcaca atggagagt 
      ttgcatact actgacaat aagaagaag aaaacaaac 
      acgaaaaat tgtaaaaat gcgtcgatt gatgctttg 
      acaattgct acagaacag acgctgaaa gacatcaac 
      aagtggtta gacgacaca ccaaaatat tcggaattt 
      agttcggcg agtaattct ccgtgttat cctcctttg 
      gatgacttc gatattgtc aataagatt gacaacgat 
      cctcggaag aaagtcgaa aaattcaat tcgaaaaag 
      gacggaatt ggtaaagat ataaagaga cgaacattt 
      agtagagat ccggcgaaa tttctcaaa cgccgggaa 
      gtgcaaagg accatagat cgtctacag cctggaaaa 
      agcaaaggc aatttgatt tcgagcgtt cagagttcg 
      aaacaaaca gacgaactg tttcctcta ggtccgcta 
      tcaaagaat aaagacaca aaaaattct ttgatcgtt 
      aaaactgac gataacgca ccaaagttg agtttaggc 
      tcggttttg gacagtttc ggacggcac aagtttgtc 
      gacgacacg aaacaggac gaatccgaa aatgcgacc 
      aaaaaagac gaaaagttt agcatcagt acggaaatt 
      aaagaggaa actcaaatt aaacccaaa ctggaagat 
      gtaaagaaa gaatctccc gtgcgagaa gaatctagt 
      atgatgagt ggagcaact ccaaattta agtgcctgg 
      tttaaagct ttcggtgcc ccgaaaatt caatcatcc 
      caaaagaaa agtgaaaag tcggaagga aaagaaagc 
      gagaaaact gacgatagt aaagttgat cccaaatcg 
      aaggaggaa gcaaaagtt ccagtccct gaacattca 
      ccgagcact gacagcccc gtggccact cacggccaa 
      cccgtgcca agacaaagg aaagtgagt actggaagt 
      agcatatcg gagcgttcg tcattcagc caagacatg 
      gactcgccc agagtggga atcgacgaa agacttggg 
      gcctacccg gccccctac ccatccccc atgcaccgt 
      tcgccctct ggggcctcg ccagtgatg gcctcgccc 
      cgtccggac gtctccccc aaagcagcc ccatatcct 
      cccttcaac ggacaaatc agagtaggg ttctaccaa 
      gacacggtc tcgaacaaa agcagccct gacaagtcg 
      tgcagcccc cgagacaat ccccagtcg ccctacgcc 
      caatgctct gaacacgtc tacacccct aataccacc 
      caaaacatg ccttatacg tatcccaac tcgccctat 
      tactcacac acaccgaat tatagcaac acgaatcct 
      acgcctccc tataatccg gatagtgtg aaccctcca 
      tcttactac gacacgaat aagtcacta acggatcag 
      taccaagcc aagaatata caaaattat gttaattcg 
      cccaataat gtgcaatca ccgtcgcca catcctcca 
      atagagacg ttccgacaa gaggatcag aaccagaac 
      aaagtgaac gagaagatg gtgcaaagt gcgatgttt 
      ccagtcaag aaacgggca tacaatgag tcagactcg 
      gcccagatg agtcacagg tacgaggaa gtcaaccga 
      aatatccaa cccatggag ggccaaaac acccagaat 
      gttgacacc atgcatatg aatgcgcgt gaggagtcc 
      atgaaacac tacatccat caaaatatc ccagaccct 
      aaaccacta acagtccag accaatgtt tcgccccaa 
      caacaactc gcctccccg attataaac tactctacg 
      ccgtcaaat ccgtacaat aactacagt aacgagtct 
      aattacccc ctaaatata ctatcgcga agtctggcc 
      tcgacccca ggcattgag cgaaaactg gacattggg 
      aaatatacc aatatgggg tactccggc cccgaggtg 
      aacttccca aggggtatg caacagtac agtcggtca 
      gagttgagc tatgcgaga cccccatcc aacgaccaa 
      caaccagta tccaaccaa ggacaacaa ccatccgag 
      gttttgcac tactcgaac gccagcccc catatgggg 
      tacaaaaac gtcgacatg aacgcagcg acttccaac 
      ccacaacaa gcacacacc aactataca aacacttcg 
      gtgcattcg aaccccaat cgtttaggg cagcctcta 
      gacgtgcag agttcgtac aagtcgaac gaagtgtcg 
      atacccagg ccgacgtac aatactcac atcgatatg 
      gaccaacct ttaggttta agagggaat atagcgaat 
      ttgtcgcat atcgtcgac cggtataat aatgacgag 
      aggatgttg accggattg caaccagca gcaagtcag 
      tattatggt gataagggc ttaggaggg ccccatatg 
      tttaataaa cccatctgt acgagctcg tcaggactg 
      ccgattttt aatcagccc aatatgata cagtcatat 
      aatcagaat atgcaagtg ccgacttct tcaatgtat 
      aataggcag atgacggaa ttgcagaat ccgatgagt 
      ggcgaagcg aagaatgcc aatgttgcg caacagggg 
      agtgagaag aagtccaag aagcggaag agtgcaaaa 
      acagcccag gccgcgtcc gcgtcagag ccgccggcg 
      caggccaac aatcagggc ttccagtcg tacgcgggc 
      ttgaagtcg acctccccc atggaacca agtgcaata 
      tcgctgaag acagcgagc gtcgtcccc ggcagcgca 
      ttcaatttc ggaccatcg ccgtcagcg ctgggcata 
      ggctcaggc ctgtacggt gacaaagat gcatatccc 
      aacttcctg gaggacttc cgcgccgcc cccaactac 
      tacatcgcg gcggcgacg gcggcccac cacagatcc 
      accccggac gcccccgac aagcaaacc cgcgtggcg 
      gcgcaccag tcgtccgcc cggcaggcc cccagttac 
      cccttcata ggcgcgcgg caggcgacg ccccctagt 
      taccccctg agcagccac ttcatgccg tctgcgcag 
      gcgcccctc atggatgcc agctcgccg ctctaccag 
      cactatctg caagcgggg gtgctgaac cagggactt 
      ttgggcccc ccagggccg tacccgccc gggtaccat 
      cccgcgcta agtatgagg caaccatac gattccatg 
      acaaggccg ccttggctc taa
    mRNA sequence
    • >TC002957 RA
      gagtcgtaa attcgttaa ttcatggct ttttgggga 
      gtttcgtcg agattaatg tgaattaac aacgttaca 
      tgtattgat caccgtata ggttatgtt tccaacttt 
      cattagcat cctagatat tcaatcatg gtgggaggt 
      tccaagcat tgcacattt ttgttcatt gtttcgaac 
      agcatttaa ccttttgga ttttgatat cgaggattt 
      ggaggaggc tctcttgac tgatgggac ggaggaaac 
      ctcttggct tcaagaact gatcgtgag gcttctcag 
      tgggtgctt gccgaacaa tgaaatctc aacgttcaa 
      ttatcagat gttcctgag acgtctctt tcgccagaa 
      atgtcagga acatgatca atacaaccc atttaacac 
      agacgttga tttccaatt gttaccttt gagaacaaa 
      agtggacat tttacatgc actctgtga ttttcgttt 
      agatgctga tgatgtgct tgaccagct gaaaaattt 
      agaagcaga cagcttgag ggttgagcc cctgggtta 
      tgacagtaa cgaatctgc ttactggta tttttacgg 
      tactagact gtacagaga ggactttaa aaccaagaa 
      taataagaa gaaatccgt gtggcaggt tatttgttt 
      cactgaaga cgattggtt tcaacttaa caagaaatt 
      taaaaaatc gactgctaa gtgtgaaag agagttaca 
      tcgtacttt aagtgagaa cttcttgcc tgaattgcc 
      cagattgtt ccgggaaaa ggaaagact ggccaggaa 
      gcgtctgct ggaaaatca accgagacg cacttctcg 
      acgtttgag aaaagaaaa tacaactga aaaagaaga 
      agtaacgtg tgaatcgcc gaaaaccaa tcctcttga 
      agaggaaga aagacaaag aaagcgcga tttggaaat 
      caagagaaa agaagctta tgagagaag taacagact 
      taaagagca gagcgaaga tagccaaag tgaggactc 
      tgaagtacg gtatgagat gggcgggac taaaacgcg 
      acaaaagaa gtccaaagg aagacaaag aaaattgtc 
      aacaagttc ctctaacag taaagatgc atcatctgc 
      gccgaagag ctcaaaaag gaagaaaac aggtgagga 
      gacttcaaa atcctcgac ttcttccgc aactacaac 
      agttggtcg acaaaccaa caattcgct ggcggcagc 
      tactggaca aattgttat ccaacctgt taccaactc 
      acacaagaa aaaactaaa aacttctca agtgtttcg 
      tcaaaccga cgaagatct aataacagg aatgcacaa 
      gatcttaga ttacgtaaa aaatcatga agacgcatg 
      gccctttgc cgatcctgt tgatgagga ttacgcacc 
      caattatta cacagttat tcggaaacc aatggattt 
      gcaaagaat ggaagagag gctggatgc aggttatta 
      caagaactt tgcaaaatt cagagacga tttccagtt 
      aatcgtgga caattgtcg actttataa cggtgttga 
      aaatgaata tacggaaat ggtcgataa tttgttgaa 
      agtatttga aagagcaac tgagaaatt tttggacca 
      aatctcatc atccgacga cgaaatcgc cgttgaatt 
      ccctaatac tgatctgga cgcaaaaga taccaaaaa 
      gaacaacaa accagaatc taaatcatc aaagcgtaa 
      accgactct tgcagctaa aacagataa aaagaggga 
      gcattcgcc ctctgaaga atctctagg taaaaggag 
      tctttcctg tgattcgct tattgagga agatgaaaa 
      gatatcgtc gaataaaaa agacaaaaa cactaagaa 
      aacaaataa agtagctaa aaaggataa aaacgacac 
      taagaaaaa cacacgaga taagaagaa ttctaatag 
      taaacacaa gaaagagaa attttcaag tcccttgag 
      aagtagaag tcctagcag gagcgttag tcgagattc 
      ctttaggcg aagttatag cagtagtcc tccgccctc 
      aagtgtccc ttccagtcc cgataacag gttcgattt 
      tttcccaaa aaaacataa gtcttttga agataagga 
      ctcggacac ccattcagg gcctaaagg taaacagaa 
      gcagaagca agacgacac aaacacatg tgatacttc 
      agcaaagaa acaaaatga caaaccggt cacatcgcc 
      atcaaaacc gaaagataa acataacaa gcttcgaga 
      aactataga aaaattaaa agccaaaag tgaaatttc 
      gaataaatt gaacgacga cgagttccg tttcgactt 
      ggatacctt taaagaaaa caaggaaaa gtccaagaa 
      agacgacaa aagtcgaaa gaagaagga taaagtgac 
      taagccaca cttgaagaa agacctcga ttttgaaga 
      tagtaatag cacaatgga gagtttgca tactactga 
      caataagaa gaagaaaac aaacacgaa aaattgtaa 
      aaatgcgtc gattgatgc tttgacaat tgctacaga 
      acagacgct gaaagacat caacaagtg gttagacga 
      cacaccaaa atattcgga atttagttc ggcgagtaa 
      ttctccgtg ttatcctcc tttggatga cttcgatat 
      tgtcaataa gattgacaa cgatcctcg gaagaaagt 
      cgaaaaatt caattcgaa aaaggacgg aattggtaa 
      agatataaa gagacgaac atttagtag agatccggc 
      gaaatttct caaacgccg ggaagtgca aaggaccat 
      agatcgtct acagcctgg aaaaagcaa aggcaattt 
      gatttcgag cgttcagag ttcgaaaca aacagacga 
      actgtttcc tctaggtcc gctatcaaa gaataaaga 
      cacaaaaaa ttctttgat cgttaaaac tgacgataa 
      cgcaccaaa gttgagttt aggctcggt tttggacag 
      tttcggacg gcacaagtt tgtcgacga cacgaaaca 
      ggacgaatc cgaaaatgc gaccaaaaa agacgaaaa 
      gtttagcat cagtacgga aattaaaga ggaaactca 
      aattaaacc caaactgga agatgtaaa gaaagaatc 
      tcccgtgcg agaagaatc tagtatgat gagtggagc 
      aactccaaa tttaagtgc ctggtttaa agctttcgg 
      tgccccgaa aattcaatc atcccaaaa gaaaagtga 
      aaagtcgga aggaaaaga aagcgagaa aactgacga 
      tagtaaagt tgatcccaa atcgaagga ggaagcaaa 
      agttccagt ccctgaaca ttcaccgag cactgacag 
      ccccgtggc cactcacgg ccaacccgt gccaagaca 
      aaggaaagt gagtactgg aagtagcat atcggagcg 
      ttcgtcatt cagccaaga catggactc gcccagagt 
      gggaatcga cgaaagact tggggccta cccggcccc 
      ctacccatc ccccatgca ccgttcgcc ctctggggc 
      ctcgccagt gatggcctc gccccgtcc ggacgtctc 
      ccccaaagc agccccata tcctccctt caacggaca 
      aatcagagt agggttcta ccaagacac ggtctcgaa 
      caaaagcag ccctgacaa gtcgtgcag cccccgaga 
      caatcccca gtcgcccta cgcccaatg ctctgaaca 
      cgtctacac ccctaatac cacccaaaa catgcctta 
      tacgtatcc caactcgcc ctattactc acacacacc 
      gaattatag caacacgaa tcctacgcc tccctataa 
      tccggatag tgtgaaccc tccatctta ctacgacac 
      gaataagtc actaacgga tcagtacca agccaagaa 
      tatacaaaa ttatgttaa ttcgcccaa taatgtgca 
      atcaccgtc gccacatcc tccaataga gacgttccg 
      acaagagga tcagaacca gaacaaagt gaacgagaa 
      gatggtgca aagtgcgat gtttccagt caagaaacg 
      ggcatacaa tgagtcaga ctcggccca gatgagtca 
      caggtacga ggaagtcaa ccgaaatat ccaacccat 
      ggagggcca aaacaccca gaatgttga caccatgca 
      tatgaatgc gcgtgagga gtccatgaa acactacat 
      ccatcaaaa tatcccaga ccctaaacc actaacagt 
      ccagaccaa tgtttcgcc ccaacaaca actcgcctc 
      cccgattat aaactactc tacgccgtc aaatccgta 
      caataacta cagtaacga gtctaatta ccccctaaa 
      tatactatc gcgaagtct ggcctcgac cccaggcat 
      tgagcgaaa actggacat tgggaaata taccaatat 
      ggggtactc cggccccga ggtgaactt cccaagggg 
      tatgcaaca gtacagtcg gtcagagtt gagctatgc 
      gagaccccc atccaacga ccaacaacc agtatccaa 
      ccaaggaca acaaccatc cgaggtttt gcactactc 
      gaacgccag cccccatat ggggtacaa aaacgtcga 
      catgaacgc agcgacttc caacccaca acaagcaca 
      caccaacta tacaaacac ttcggtgca ttcgaaccc 
      caatcgttt agggcagcc tctagacgt gcagagttc 
      gtacaagtc gaacgaagt gtcgatacc caggccgac 
      gtacaatac tcacatcga tatggacca acctttagg 
      tttaagagg gaatatagc gaatttgtc gcatatcgt 
      cgaccggta taataatga cgagaggat gttgaccgg 
      attgcaacc agcagcaag tcagtatta tggtgataa 
      gggcttagg agggcccca tatgtttaa taaacccat 
      ctgtacgag ctcgtcagg actgccgat ttttaatca 
      gcccaatat gatacagtc atataatca gaatatgca 
      agtgccgac ttcttcaat gtataatag gcagatgac 
      ggaattgca gaatccgat gagtggcga agcgaagaa 
      tgccaatgt tgcgcaaca ggggagtga gaagaagtc 
      caagaagcg gaagagtgc aaaaacagc ccaggccgc 
      gtccgcgtc agagccgcc ggcgcaggc caacaatca 
      gggcttcca gtcgtacgc gggcttgaa gtcgacctc 
      ccccatgga accaagtgc aatatcgct gaagacagc 
      gagcgtcgt ccccggcag cgcattcaa tttcggacc 
      atcgccgtc agcgctggg cataggctc aggcctgta 
      cggtgacaa agatgcata tcccaactt cctggagga 
      cttccgcgc cgcccccaa ctactacat cgcggcggc 
      gacggcggc ccaccacag atccacccc ggacgcccc 
      cgacaagca aacccgcgt ggcggcgca ccagtcgtc 
      cgcccggca ggcccccag ttacccctt cataggcgc 
      gcggcaggc gacgccccc tagttaccc cctgagcag 
      ccacttcat gccgtctgc gcaggcgcc cctcatgga 
      tgccagctc gccgctcta ccagcacta tctgcaagc 
      gggggtgct gaaccaggg acttttggg ccccccagg 
      gccgtaccc gcccgggta ccatcccgc gctaagtat 
      gaggcaacc atacgattc catgacaag gccgccttg 
      gctctaatc gacggtttg tttttcggg aaagttatt 
      agtgattgt ttttatttg ccaaagaca gtttattcc 
      gtttgttaa attgtacat taaatttta ttttggaaa 
      ttcgacgat tttgcgatt attgtttta tttttttat 
      agcggcgtg cggaaagct ctgtgattt actttacgt 
      gtgatcgta aatattttt gttatgtct ttttcatgt 
      ttatggatt cactgtcaa tagtttttc gtaactgtt 
      agcatcgga ccattattt tacctcatg ttttatatc 
      atagtatat cgaactttt gtagtgctt acaatagtt 
      ctgtgttgt ttattttaa aatgaaatt gttgtatac 
      tgggtaact ctgaccaga agttgtctt cgattaaca 
      tgttgctat acggtcggg tgtatccgc aaacattcc 
      caaattttg tgcaaaata tctacttac ctagactaa 
      ggttcttaa tagcagaga tatatgtca atgaattta 
      ttattcatc acggtttga tttatgttt atacattta 
      tgtatagta cataaatac acaacttgt gttaat
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