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Gene information
General information
TC003476 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5680370..5683084
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003476
    iBeetle screen iB_07967

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003476 RA
      MPQRKKPFS GKAKKEQLK AKKQSKQNA PQEGFGSRN 
RRPLLKTNA GDSEDQPQI QKFNYCPPK DGRDRNRYA 
LQFYRETPA ELKARKEEA CKTIEPVSE KELEIDSND 
YFPKDLGFP RRPPWSYEL TTEQLEARE TKYFNDYVN 
GILEKYNWK ELSFFELNL ETWRQLWRV LEMSDIVLH 
IVDIRYPAH MFPPSLYEY VTETLKKDF ILVLNKIDL 
APAPLVVAW KKYFEENYP NLHICMFTT LPGYNLVGT 
QVNRAGLQV RKRKGKFRL AAEGSQQLF EVCKTIVGD 
NIDISSWQT KIKEEMNLE YENDEDEKV EIGETVQMK 
AVDTSYFEH EKYKNGILT IGCLGQPNV GKSSLINAI 
MGKKVVSVS RTPGHTKHF QTIFLTPNV RLCDCPGLV 
FPSKTPKVI QVLMGSFPI AQLREPFTT IKYLAERMD 
LPNMLRIEH PENDDTWCA MDICDGWAK KRGFLTAKA 
ARLDSYRAA NDLLRMALD GKICLCLRP PKYSNKKDY 
WASHPEIEK VKWIQAKSD DSTQLADDK DYLSDASDE 
DNVPGSSGD HKDRSSEES EDEETDSDE EPTNYAHNK 
FAALADDD
    CDS sequence
    • >TC003476 RA
      atgcctcag cgcaagaaa ccgttcagt ggcaaagcc 
aagaaagag cagctaaag gccaaaaaa cagtccaaa 
cagaacgcc cctcaagaa ggttttggt tcccggaac 
cggcggcct ttgctgaag accaacgcc ggtgatagt 
gaggatcag ccccaaatt caaaaattc aattactgt 
ccccctaag gacgggcga gaccgcaac cgttatgcc 
ttgcagttt taccgcgag actcccgct gagctcaaa 
gcacgtaaa gaagaggct tgcaaaacc atagaacct 
gtctctgag aaagagctg gaaattgac tcaaatgac 
tattttccc aaagacttg ggctttcct cgaaggcct 
ccgtggtca tatgaactc acgactgaa cagctcgaa 
gcacgcgaa accaaatat tttaatgat tatgttaat 
ggaatcttg gaaaagtat aattggaaa gagttaagt 
ttctttgaa ctgaacttg gagacgtgg cggcagttg 
tggcgtgtt ctcgaaatg tcagatatt gttctgcac 
attgttgat atcagatat cccgcccac atgtttccc 
ccatctttg tacgaatac gtgactgaa acattgaag 
aaagacttt attttagta ttgaataaa attgatttg 
gccccagcg ccgttagtc gtggcgtgg aaaaaatac 
tttgaggag aattacccc aatttgcac atttgcatg 
tttaccaca ctccctggc tataatcta gttggcacc 
caagtgaac agagctggt ctccaagta cgtaaacgc 
aaaggcaag ttccgattg gctgcagaa gggagtcaa 
cagttgttt gaagtttgt aaaaccata gttggggat 
aacatagac ataagttct tggcaaact aaaataaag 
gaagaaatg aatttggaa tacgaaaac gatgaagac 
gagaaagtt gaaattgga gaaacagtt caaatgaaa 
gcagtggac acgagttac ttcgaacac gaaaaatac 
aaaaacggt atcctcaca atcggctgc ttgggccaa 
cccaacgtc ggcaaatcg tcacttatt aacgcaatc 
atgggcaag aaagtcgtc agcgtgtcc agaactcca 
ggccatacg aaacacttc caaacaatt tttttaaca 
cccaatgtg cggctatgt gactgtcct ggtctagtc 
tttccgtcg aaaacacca aaggttata caggttttg 
atggggagt ttccccatc gcccaactt agggagccg 
tttacaacg attaagtat ttagcggaa cgcatggat 
ctaccaaat atgctgagg attgaacat ccagaaaat 
gacgatact tggtgtgca atggacatt tgcgatggc 
tgggccaaa aaacggggt tttctcaca gccaaagcc 
gccaggttg gacagctac agagctgcg aatgattta 
ctgaggatg gctttggac gggaaaatt tgcctgtgt 
ctcaggcca cctaagtac tcaaataaa aaagattat 
tgggcctca cacccggag atcgagaaa gtgaagtgg 
attcaggcc aaatctgac gattccact cagctcgca 
gacgacaaa gactacctc tctgatgct tcagatgaa 
gacaacgtc ccgggctcc tccggtgac cacaaagac 
cgaagcagt gaagaaagc gaagacgaa gaaactgac 
tcagatgaa gagccgacg aattacgcc cacaataaa 
tttgcagcg ttggcagac gacgattga
    mRNA sequence
    • >TC003476 RA
      ttctagtaa cttccatgt gattttcta gaattcgtt 
gattttaaa ataaaaagt caggttcac agcacaata 
agtaaaatg cctcagcgc aagaaaccg ttcagtggc 
aaagccaag aaagagcag ctaaaggcc aaaaaacag 
tccaaacag aacgcccct caagaaggt tttggttcc 
cggaaccgg cggcctttg ctgaagacc aacgccggt 
gatagtgag gatcagccc caaattcaa aaattcaat 
tactgtccc cctaaggac gggcgagac cgcaaccgt 
tatgccttg cagttttac cgcgagact cccgctgag 
ctcaaagca cgtaaagaa gaggcttgc aaaaccata 
gaacctgtc tctgagaaa gagctggaa attgactca 
aatgactat tttcccaaa gacttgggc tttcctcga 
aggcctccg tggtcatat gaactcacg actgaacag 
ctcgaagca cgcgaaacc aaatatttt aatgattat 
gttaatgga atcttggaa aagtataat tggaaagag 
ttaagtttc tttgaactg aacttggag acgtggcgg 
cagttgtgg cgtgttctc gaaatgtca gatattgtt 
ctgcacatt gttgatatc agatatccc gcccacatg 
tttccccca tctttgtac gaatacgtg actgaaaca 
ttgaagaaa gactttatt ttagtattg aataaaatt 
gatttggcc ccagcgccg ttagtcgtg gcgtggaaa 
aaatacttt gaggagaat taccccaat ttgcacatt 
tgcatgttt accacactc cctggctat aatctagtt 
ggcacccaa gtgaacaga gctggtctc caagtacgt 
aaacgcaaa ggcaagttc cgattggct gcagaaggg 
agtcaacag ttgtttgaa gtttgtaaa accatagtt 
ggggataac atagacata agttcttgg caaactaaa 
ataaaggaa gaaatgaat ttggaatac gaaaacgat 
gaagacgag aaagttgaa attggagaa acagttcaa 
atgaaagca gtggacacg agttacttc gaacacgaa 
aaatacaaa aacggtatc ctcacaatc ggctgcttg 
ggccaaccc aacgtcggc aaatcgtca cttattaac 
gcaatcatg ggcaagaaa gtcgtcagc gtgtccaga 
actccaggc catacgaaa cacttccaa acaattttt 
ttaacaccc aatgtgcgg ctatgtgac tgtcctggt 
ctagtcttt ccgtcgaaa acaccaaag gttatacag 
gttttgatg gggagtttc cccatcgcc caacttagg 
gagccgttt acaacgatt aagtattta gcggaacgc 
atggatcta ccaaatatg ctgaggatt gaacatcca 
gaaaatgac gatacttgg tgtgcaatg gacatttgc 
gatggctgg gccaaaaaa cggggtttt ctcacagcc 
aaagccgcc aggttggac agctacaga gctgcgaat 
gatttactg aggatggct ttggacggg aaaatttgc 
ctgtgtctc aggccacct aagtactca aataaaaaa 
gattattgg gcctcacac ccggagatc gagaaagtg 
aagtggatt caggccaaa tctgacgat tccactcag 
ctcgcagac gacaaagac tacctctct gatgcttca 
gatgaagac aacgtcccg ggctcctcc ggtgaccac 
aaagaccga agcagtgaa gaaagcgaa gacgaagaa 
actgactca gatgaagag ccgacgaat tacgcccac 
aataaattt gcagcgttg gcagacgac gattgaaac 
aaccacgat gttgagtat ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY