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Gene information
General information
TC003466 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5521210..5523917
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003466
    iBeetle screen iB_07965

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003466 RA
      MSSREIILT LKDGVRVIR INRPKTKNA FDSNVYTTI 
      TNTLTEDAQ NDNVVVTII TGTGEYYSS GFDIKSAMS 
      KFGGGDTRG VDELKAMIN AFIIYPKLL IALVNGPAI 
      GIAVTTAAL CDIVYASER ATFETPFLR IGLCAEGCS 
      SYNFPNILG RSKASEMLF LGKKMTAQE AYQFGFVAE 
      VIPHEQLDQ FAHKLMTLG KELSVNGVK ITKKLILNN 
      YRNVLCECN ERELDQLVE CFGSEEFSE GIMKFMSRK 
      SKL
    CDS sequence
    • >TC003466 RA
      atgtcttca agggaaata atcctcacc cttaaggac 
      ggagtccgg gtgatccgc atcaaccgg cccaaaaca 
      aaaaacgca tttgactca aacgtctac acaacaatc 
      accaacaca ttaactgaa gacgcccaa aacgacaac 
      gtggtggtg accatcatc acgggcaca ggggaatat 
      tacagcagc ggtttcgac attaaaagc gccatgagc 
      aagttcggg ggcggcgat accagaggg gtagacgag 
      ctgaaagcc atgattaac gccttcatc atttacccc 
      aaactgtta atcgctcta gtcaatggt cctgcaatt 
      ggcattgca gtcacaact gcagccttg tgcgatatc 
      gtgtacgcg tccgaaagg gcaactttt gagacaccc 
      ttccttcgg ataggactg tgtgccgag ggatgctca 
      agctacaac ttcccgaat atcttgggt cggagcaag 
      gcctcggag atgctcttt ctggggaag aaaatgacg 
      gcgcaggaa gcgtatcag tttggattt gttgctgaa 
      gtcattccg catgaacaa ctggaccag tttgcacac 
      aaactgatg acgcttggg aaggaatta tcagtgaat 
      ggagtcaaa atcaccaaa aagttgatt cttaataat 
      tacagaaac gtgctgtgt gaatgtaac gaaagggag 
      ttggatcag ttggttgaa tgttttggc tcggaggag 
      ttttccgag ggcattatg aaatttatg tctcggaaa 
      agtaaattg tag
    mRNA sequence
    • >TC003466 RA
      aagcagaaa catttgttt acaatcaga aacccgtca 
      gattactcc ctttgcttt tgcaaaaag gtttttaac 
      aattctggg tttcattat tgtttaagt tgcacccaa 
      tatttctgt tgtgtacca atacatttg aagattcaa 
      ggtgttgtc aaggtatct acatcaaaa tgagtggac 
      ctcgtatgg gaacatccc aaatgaacg gcaacaaca 
      ggcgcccaa cagacttca accgaatgc cctcaagtg 
      ataaaccag tcggaagcc aacatgacg atctgatac 
      gatacattt ttgattcct ggactaagg tttcccaag 
      aagttgaca gaaatacag gcacttctg ttatctttt 
      accaagagc aagaaaacc agaacctta aagatttcg 
      aaccctttg atttagagg ggttctggg ggcgaagag 
      tggtccaga atttccagc agtctcaca attcatagc 
      agttcgtgt gaggacgcc accatttat gttatattt 
      ataataatt ggagttatt gctcatttg atccacaaa 
      tttcaatgt acagtttat aatattttg caatctgct 
      caactctct aatttaatg ttgtgaaat gtggaggaa 
      attgaacgc cgtttacca aggaacgtg tccacaaac 
      aatacaaat tttatcagt aatctcatg tttgcaagg 
      caaaaatga aacaaagcg cttaattca ttgacctta 
      cgatttttt atacatcaa cgcgagcca tatttcgta 
      aatgtgcgc tcgtaattg ctctgttta caacaaatt 
      atcagcttt taaacagtg gatcaagtc gtatttata 
      agttttata tttattatt tatattttg tgcagtatt 
      ttattattt tacattccc cttacgaaa tatttgaat 
      ttacaaatt aatgctgaa tcgtttgca gaagaaatt 
      gtgcataaa attattgta ccattaaaa taaaatgtt 
      tttatttag ccgatttta ttgttactt ttccctgta 
      ttccaagta tcattttta acgtcctca gatttttta 
      ttaattagt ttactttta taataaatg caaatgaaa 
      atcacaaat cacagtggt ggttgactt aagctttgt 
      ctcttattt acttttgat tattctatt gacgttaat 
      aacataact atgaactga taaggaaaa taattatta 
      ctcatgtta aataaattt gtcgtgact tttcccttt 
      aaacaattg aggctttgg ctcgttcgt cttgtaaaa 
      tatttgtcc attagatta gtaatagga taaggacat 
      acaatacca taatgtata attctttta agtgccaaa 
      gacaaaaat attaacatt aatttttat aatttaaca 
      aattttatt tttaaggat ccatttaac accagaaat 
      tgttaggat ttcataggt gggtttcga aaccagtta 
      ttatcacat ctgcaattt ccataactt attttatat 
      tagttttag aattgtttg gaggttatg tatcttttt 
      cgataaata ataaataat tagactgta aaaacaaaa 
      aactactat aaaacaggt acatcatct attatattg 
      attatctac atttgtcta ttattaatt ttgtacctt 
      ttgcagtta cgcacataa aagtagctg ttttgctaa 
      aaaccagag gactttgga attattaat tattctaat 
      aataaaaaa ctgcattgt aataataaa ttggtgatt 
      aatgattca gcactgttg tgaactctg agataaatt 
      tcaaagtgg tgtttacgc ttgtgtcgg gtggcatcc 
      aagattgga aatgtcttc aagggaaat aatcctcac 
      ccttaagga cggagtccg ggtgatccg catcaaccg 
      gcccaaaac aaaaaacgc atttgactc aaacgtcta 
      cacaacaat caccaacac attaactga agacgccca 
      aaacgacaa cgtggtggt gaccatcat cacgggcac 
      aggggaata ttacagcag cggtttcga cattaaaag 
      cgccatgag caagttcgg gggcggcga taccagagg 
      ggtagacga gctgaaagc catgattaa cgccttcat 
      catttaccc caaactgtt aatcgctct agtcaatgg 
      tcctgcaat tggcattgc agtcacaac tgcagcctt 
      gtgcgatat cgtgtacgc gtccgaaag ggcaacttt 
      tgagacacc cttccttcg gataggact gtgtgccga 
      gggatgctc aagctacaa cttcccgaa tatcttggg 
      tcggagcaa ggcctcgga gatgctctt tctggggaa 
      gaaaatgac ggcgcagga agcgtatca gtttggatt 
      tgttgctga agtcattcc gcatgaaca actggacca 
      gtttgcaca caaactgat gacgcttgg gaaggaatt 
      atcagtgaa tggagtcaa aatcaccaa aaagttgat 
      tcttaataa ttacagaaa cgtgctgtg tgaatgtaa 
      cgaaaggga gttggatca gttggttga atgttttgg 
      ctcggagga gttttccga gggcattat gaaatttat 
      gtctcggaa aagtaaatt gtagttttt acacataac 
      ttgttaata aaactgttg gggataaaa gaaataaat 
      ttgtttaac atgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY