Status
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Gene information
General information
TC003466 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5521210..5523917
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003466
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_07965

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003466 RA
      MSSREIILT LKDGVRVIR INRPKTKNA FDSNVYTTI 
TNTLTEDAQ NDNVVVTII TGTGEYYSS GFDIKSAMS 
KFGGGDTRG VDELKAMIN AFIIYPKLL IALVNGPAI 
GIAVTTAAL CDIVYASER ATFETPFLR IGLCAEGCS 
SYNFPNILG RSKASEMLF LGKKMTAQE AYQFGFVAE 
VIPHEQLDQ FAHKLMTLG KELSVNGVK ITKKLILNN 
YRNVLCECN ERELDQLVE CFGSEEFSE GIMKFMSRK 
SKL
    CDS sequence
    • >TC003466 RA
      atgtcttca agggaaata atcctcacc cttaaggac 
ggagtccgg gtgatccgc atcaaccgg cccaaaaca 
aaaaacgca tttgactca aacgtctac acaacaatc 
accaacaca ttaactgaa gacgcccaa aacgacaac 
gtggtggtg accatcatc acgggcaca ggggaatat 
tacagcagc ggtttcgac attaaaagc gccatgagc 
aagttcggg ggcggcgat accagaggg gtagacgag 
ctgaaagcc atgattaac gccttcatc atttacccc 
aaactgtta atcgctcta gtcaatggt cctgcaatt 
ggcattgca gtcacaact gcagccttg tgcgatatc 
gtgtacgcg tccgaaagg gcaactttt gagacaccc 
ttccttcgg ataggactg tgtgccgag ggatgctca 
agctacaac ttcccgaat atcttgggt cggagcaag 
gcctcggag atgctcttt ctggggaag aaaatgacg 
gcgcaggaa gcgtatcag tttggattt gttgctgaa 
gtcattccg catgaacaa ctggaccag tttgcacac 
aaactgatg acgcttggg aaggaatta tcagtgaat 
ggagtcaaa atcaccaaa aagttgatt cttaataat 
tacagaaac gtgctgtgt gaatgtaac gaaagggag 
ttggatcag ttggttgaa tgttttggc tcggaggag 
ttttccgag ggcattatg aaatttatg tctcggaaa 
agtaaattg tag
    mRNA sequence
    • >TC003466 RA
      aagcagaaa catttgttt acaatcaga aacccgtca 
gattactcc ctttgcttt tgcaaaaag gtttttaac 
aattctggg tttcattat tgtttaagt tgcacccaa 
tatttctgt tgtgtacca atacatttg aagattcaa 
ggtgttgtc aaggtatct acatcaaaa tgagtggac 
ctcgtatgg gaacatccc aaatgaacg gcaacaaca 
ggcgcccaa cagacttca accgaatgc cctcaagtg 
ataaaccag tcggaagcc aacatgacg atctgatac 
gatacattt ttgattcct ggactaagg tttcccaag 
aagttgaca gaaatacag gcacttctg ttatctttt 
accaagagc aagaaaacc agaacctta aagatttcg 
aaccctttg atttagagg ggttctggg ggcgaagag 
tggtccaga atttccagc agtctcaca attcatagc 
agttcgtgt gaggacgcc accatttat gttatattt 
ataataatt ggagttatt gctcatttg atccacaaa 
tttcaatgt acagtttat aatattttg caatctgct 
caactctct aatttaatg ttgtgaaat gtggaggaa 
attgaacgc cgtttacca aggaacgtg tccacaaac 
aatacaaat tttatcagt aatctcatg tttgcaagg 
caaaaatga aacaaagcg cttaattca ttgacctta 
cgatttttt atacatcaa cgcgagcca tatttcgta 
aatgtgcgc tcgtaattg ctctgttta caacaaatt 
atcagcttt taaacagtg gatcaagtc gtatttata 
agttttata tttattatt tatattttg tgcagtatt 
ttattattt tacattccc cttacgaaa tatttgaat 
ttacaaatt aatgctgaa tcgtttgca gaagaaatt 
gtgcataaa attattgta ccattaaaa taaaatgtt 
tttatttag ccgatttta ttgttactt ttccctgta 
ttccaagta tcattttta acgtcctca gatttttta 
ttaattagt ttactttta taataaatg caaatgaaa 
atcacaaat cacagtggt ggttgactt aagctttgt 
ctcttattt acttttgat tattctatt gacgttaat 
aacataact atgaactga taaggaaaa taattatta 
ctcatgtta aataaattt gtcgtgact tttcccttt 
aaacaattg aggctttgg ctcgttcgt cttgtaaaa 
tatttgtcc attagatta gtaatagga taaggacat 
acaatacca taatgtata attctttta agtgccaaa 
gacaaaaat attaacatt aatttttat aatttaaca 
aattttatt tttaaggat ccatttaac accagaaat 
tgttaggat ttcataggt gggtttcga aaccagtta 
ttatcacat ctgcaattt ccataactt attttatat 
tagttttag aattgtttg gaggttatg tatcttttt 
cgataaata ataaataat tagactgta aaaacaaaa 
aactactat aaaacaggt acatcatct attatattg 
attatctac atttgtcta ttattaatt ttgtacctt 
ttgcagtta cgcacataa aagtagctg ttttgctaa 
aaaccagag gactttgga attattaat tattctaat 
aataaaaaa ctgcattgt aataataaa ttggtgatt 
aatgattca gcactgttg tgaactctg agataaatt 
tcaaagtgg tgtttacgc ttgtgtcgg gtggcatcc 
aagattgga aatgtcttc aagggaaat aatcctcac 
ccttaagga cggagtccg ggtgatccg catcaaccg 
gcccaaaac aaaaaacgc atttgactc aaacgtcta 
cacaacaat caccaacac attaactga agacgccca 
aaacgacaa cgtggtggt gaccatcat cacgggcac 
aggggaata ttacagcag cggtttcga cattaaaag 
cgccatgag caagttcgg gggcggcga taccagagg 
ggtagacga gctgaaagc catgattaa cgccttcat 
catttaccc caaactgtt aatcgctct agtcaatgg 
tcctgcaat tggcattgc agtcacaac tgcagcctt 
gtgcgatat cgtgtacgc gtccgaaag ggcaacttt 
tgagacacc cttccttcg gataggact gtgtgccga 
gggatgctc aagctacaa cttcccgaa tatcttggg 
tcggagcaa ggcctcgga gatgctctt tctggggaa 
gaaaatgac ggcgcagga agcgtatca gtttggatt 
tgttgctga agtcattcc gcatgaaca actggacca 
gtttgcaca caaactgat gacgcttgg gaaggaatt 
atcagtgaa tggagtcaa aatcaccaa aaagttgat 
tcttaataa ttacagaaa cgtgctgtg tgaatgtaa 
cgaaaggga gttggatca gttggttga atgttttgg 
ctcggagga gttttccga gggcattat gaaatttat 
gtctcggaa aagtaaatt gtagttttt acacataac 
ttgttaata aaactgttg gggataaaa gaaataaat 
ttgtttaac atgg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY