General information | |||
TC003466 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 5521210..5523917 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003466 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_07965 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC003466 RA
MSSREIILT LKDGVRVIR INRPKTKNA FDSNVYTTI TNTLTEDAQ NDNVVVTII TGTGEYYSS GFDIKSAMS KFGGGDTRG VDELKAMIN AFIIYPKLL IALVNGPAI GIAVTTAAL CDIVYASER ATFETPFLR IGLCAEGCS SYNFPNILG RSKASEMLF LGKKMTAQE AYQFGFVAE VIPHEQLDQ FAHKLMTLG KELSVNGVK ITKKLILNN YRNVLCECN ERELDQLVE CFGSEEFSE GIMKFMSRK SKL
- >TC003466 RA
atgtcttca agggaaata atcctcacc cttaaggac ggagtccgg gtgatccgc atcaaccgg cccaaaaca aaaaacgca tttgactca aacgtctac acaacaatc accaacaca ttaactgaa gacgcccaa aacgacaac gtggtggtg accatcatc acgggcaca ggggaatat tacagcagc ggtttcgac attaaaagc gccatgagc aagttcggg ggcggcgat accagaggg gtagacgag ctgaaagcc atgattaac gccttcatc atttacccc aaactgtta atcgctcta gtcaatggt cctgcaatt ggcattgca gtcacaact gcagccttg tgcgatatc gtgtacgcg tccgaaagg gcaactttt gagacaccc ttccttcgg ataggactg tgtgccgag ggatgctca agctacaac ttcccgaat atcttgggt cggagcaag gcctcggag atgctcttt ctggggaag aaaatgacg gcgcaggaa gcgtatcag tttggattt gttgctgaa gtcattccg catgaacaa ctggaccag tttgcacac aaactgatg acgcttggg aaggaatta tcagtgaat ggagtcaaa atcaccaaa aagttgatt cttaataat tacagaaac gtgctgtgt gaatgtaac gaaagggag ttggatcag ttggttgaa tgttttggc tcggaggag ttttccgag ggcattatg aaatttatg tctcggaaa agtaaattg tag
- >TC003466 RA
aagcagaaa catttgttt acaatcaga aacccgtca gattactcc ctttgcttt tgcaaaaag gtttttaac aattctggg tttcattat tgtttaagt tgcacccaa tatttctgt tgtgtacca atacatttg aagattcaa ggtgttgtc aaggtatct acatcaaaa tgagtggac ctcgtatgg gaacatccc aaatgaacg gcaacaaca ggcgcccaa cagacttca accgaatgc cctcaagtg ataaaccag tcggaagcc aacatgacg atctgatac gatacattt ttgattcct ggactaagg tttcccaag aagttgaca gaaatacag gcacttctg ttatctttt accaagagc aagaaaacc agaacctta aagatttcg aaccctttg atttagagg ggttctggg ggcgaagag tggtccaga atttccagc agtctcaca attcatagc agttcgtgt gaggacgcc accatttat gttatattt ataataatt ggagttatt gctcatttg atccacaaa tttcaatgt acagtttat aatattttg caatctgct caactctct aatttaatg ttgtgaaat gtggaggaa attgaacgc cgtttacca aggaacgtg tccacaaac aatacaaat tttatcagt aatctcatg tttgcaagg caaaaatga aacaaagcg cttaattca ttgacctta cgatttttt atacatcaa cgcgagcca tatttcgta aatgtgcgc tcgtaattg ctctgttta caacaaatt atcagcttt taaacagtg gatcaagtc gtatttata agttttata tttattatt tatattttg tgcagtatt ttattattt tacattccc cttacgaaa tatttgaat ttacaaatt aatgctgaa tcgtttgca gaagaaatt gtgcataaa attattgta ccattaaaa taaaatgtt tttatttag ccgatttta ttgttactt ttccctgta ttccaagta tcattttta acgtcctca gatttttta ttaattagt ttactttta taataaatg caaatgaaa atcacaaat cacagtggt ggttgactt aagctttgt ctcttattt acttttgat tattctatt gacgttaat aacataact atgaactga taaggaaaa taattatta ctcatgtta aataaattt gtcgtgact tttcccttt aaacaattg aggctttgg ctcgttcgt cttgtaaaa tatttgtcc attagatta gtaatagga taaggacat acaatacca taatgtata attctttta agtgccaaa gacaaaaat attaacatt aatttttat aatttaaca aattttatt tttaaggat ccatttaac accagaaat tgttaggat ttcataggt gggtttcga aaccagtta ttatcacat ctgcaattt ccataactt attttatat tagttttag aattgtttg gaggttatg tatcttttt cgataaata ataaataat tagactgta aaaacaaaa aactactat aaaacaggt acatcatct attatattg attatctac atttgtcta ttattaatt ttgtacctt ttgcagtta cgcacataa aagtagctg ttttgctaa aaaccagag gactttgga attattaat tattctaat aataaaaaa ctgcattgt aataataaa ttggtgatt aatgattca gcactgttg tgaactctg agataaatt tcaaagtgg tgtttacgc ttgtgtcgg gtggcatcc aagattgga aatgtcttc aagggaaat aatcctcac ccttaagga cggagtccg ggtgatccg catcaaccg gcccaaaac aaaaaacgc atttgactc aaacgtcta cacaacaat caccaacac attaactga agacgccca aaacgacaa cgtggtggt gaccatcat cacgggcac aggggaata ttacagcag cggtttcga cattaaaag cgccatgag caagttcgg gggcggcga taccagagg ggtagacga gctgaaagc catgattaa cgccttcat catttaccc caaactgtt aatcgctct agtcaatgg tcctgcaat tggcattgc agtcacaac tgcagcctt gtgcgatat cgtgtacgc gtccgaaag ggcaacttt tgagacacc cttccttcg gataggact gtgtgccga gggatgctc aagctacaa cttcccgaa tatcttggg tcggagcaa ggcctcgga gatgctctt tctggggaa gaaaatgac ggcgcagga agcgtatca gtttggatt tgttgctga agtcattcc gcatgaaca actggacca gtttgcaca caaactgat gacgcttgg gaaggaatt atcagtgaa tggagtcaa aatcaccaa aaagttgat tcttaataa ttacagaaa cgtgctgtg tgaatgtaa cgaaaggga gttggatca gttggttga atgttttgg ctcggagga gttttccga gggcattat gaaatttat gtctcggaa aagtaaatt gtagttttt acacataac ttgttaata aaactgttg gggataaaa gaaataaat ttgtttaac atgg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
