Status
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Gene information
General information
TC003001 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4997910..5005355
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003001
    iBeetle screen iB_07961

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003001 RA
      MGLTKQYLR YVPAGNFNI IASQNCNLK FVTLEGQEG 
      RFVAVGACE HVIIWDLRL GEKAQVLPG EKATVTAIC 
      ASPNKRHLA VGYSDGNVQ VYDLKSAEV ISIFSGHRS 
      EVTTLAFDS YGHKLASGS KDTDVIVWD TVAETGLCR 
      LSGHKGPIT QIAFMSQHN ILISGSKDS FVKFWDLDT 
      QHCFKTLTG HRTEVWGLS LVKDDQYLV TGCGDAELR 
      VWKLSQKDP DSENKSSID HLTSTLELI DLHESDDLN 
      HPLQCHKIG SLLRAGKGR VVSMFCDGS GQVIGCHGT 
      DTQLELFYF CTDDEAQVR LKKRLKKER KKNKELESN 
      DQDQETQSQ DLQLTLRDQ VKRLVPIKL PNKPKSLDL 
      VLGNGGELR VSVNLSNNT VELYTIQTA VKNCEATCL 
      RSILNQGHH SEVRSVCFS NDNLAIASG SAEAIKMWN 
      RPSQACLRT VETGYILSC IFVPGDRHV LAGLKNGTL 
      LIIDIAAGD ILEEIPAHS DELWAICLQ PDQRGCVTG 
      GGDKTVKFW QFELVVDEK SESKAKVLS LLHVKTLKL 
      EDSVLCVKI SPNGKFIAV GLLDTTVKI FFVDTLKFY 
      LSLYGHKLP VLCLDISSD SALIATGSA DRNVKIWGM 
      DFGDCHKSI FAHDDSVTG LQFVPRTHY FFTCGKDGK 
      VKQWDGDSY DKIITLQGH AGEAWALAV SPNGQFVVS 
      CGADRVLRL YEKSEQPLV LQDEQEEER EQQEVLATG 
      EQTVIPGQS GLNLPSKKT VGSEKAAES ILECLEICE 
      KYKEQLAEH EALQAASSR TLAPPQPPL LMQALGVST 
      PDDFLLETI RRIRPSDLE EALLLLPFS SVCEVLQIL 
      PALIARGDQ VELVGKICI FLLKLHHAP IVANHTLLV 
      TLKQLQKLA FAKVQELRD LVGYNLYGM QFIQREIEA 
      AEGVQLFRD ATLEKRKGD KKRKQREKL KRSMIVLNS
    CDS sequence
    • >TC003001 RA
      atgggttta acaaagcaa tacctaaga tacgtcccg 
      gccggcaac tttaatata atcgcgagc caaaactgc 
      aatctcaaa tttgtcaca cttgaagga caagaagga 
      agatttgtg gcggtcggt gcatgcgaa catgtgata 
      atttgggac ttgcgactc ggggagaaa gctcaggtg 
      ctcccagga gaaaaggcc acagtgaca gccatctgt 
      gccagcccc aataaaaga cacctagcg gtcggttac 
      tccgatggc aacgtccaa gtctacgat ttgaagtcg 
      gccgaagtg attagcatt ttctcaggc catcggtca 
      gaagtcaca actttggct ttcgatagt tacggacac 
      aaattggcc tcaggatct aaagacacg gacgttatc 
      gtgtgggat acggtggcc gaaactggg ctttgtaga 
      ctctcaggt cacaagggc ccaattacc caaatcgca 
      ttcatgtcc caacacaat atcttgatc agtggctcc 
      aaagacagc ttcgtcaaa ttctgggat ttggacacc 
      caacactgt ttcaagaca ctcacaggc cacaggact 
      gaagtctgg ggcttgagt ttggttaaa gacgaccag 
      tatttggta acaggttgt ggggacgca gaattgagg 
      gtttggaag ttatcacaa aaagacccc gattctgaa 
      aacaagagc tcaattgac catttgaca tcaacgttg 
      gaactcatt gatttacat gaatcagac gacttaaat 
      cacccttta caatgtcac aaaataggg tccttgttg 
      agagcgggc aaaggccga gtggtctca atgttctgt 
      gacggcagc ggtcaagtg attggctgc cacggcact 
      gatacacaa ctggaatta ttttatttt tgcacagac 
      gatgaggca caagtgcgt ttaaaaaaa cgactcaag 
      aaggagaga aagaagaac aaagaattg gaatctaac 
      gaccaagac caagagacg caaagccag gacttgcag 
      ctgacttta agggatcaa gtgaaacga ttggttcca 
      atcaaatta ccaaataaa cccaagtcg ttggatttg 
      gtgcttgga aatgggggc gaattgcga gtctctgtt 
      aatttatcc aacaacacc gttgaattg tacacaatt 
      cagacggct gtgaaaaac tgtgaagcc acgtgtttg 
      agatctatc ctcaatcag ggacatcac agtgaagtt 
      agatctgtt tgtttcagt aatgataat ttagcgatt 
      gcgtccgga agtgctgag gccatcaag atgtggaat 
      cggccttcg caagcctgt ctcaggacg gtggaaaca 
      gggtacatt ttatcttgc atctttgtc cccggcgat 
      cgtcacgtc ttggcaggt ctgaagaat ggcactttg 
      ctcatcatc gacattgcg gcgggggat attttggag 
      gagattccc gcacacagc gacgaactc tgggccatt 
      tgtttacaa ccagaccaa aggggttgt gtgactgga 
      gggggcgac aagaccgtc aaattctgg caattcgag 
      ttagtggtc gacgagaaa agcgaaagc aaagccaaa 
      gtgctttca ctcctccac gtcaagacg ctgaagctc 
      gaggactca gttctatgc gtcaaaatc tcgcccaat 
      ggcaaattc atcgcagtg gggcttttg gacacgaca 
      gtcaagata tttttcgtc gatacttta aagttttat 
      ctctcgtta tacggccac aaactgcca gtcctttgc 
      ttggacatt tcgtccgat tctgcactg attgccaca 
      gggtcagct gaccgcaat gtaaaaatc tggggaatg 
      gacttcggt gactgccac aagagtatt tttgcccac 
      gacgattcg gtcactggg ctacagttc gttccgcgg 
      acccattac ttcttcacc tgcgggaaa gacgggaag 
      gtcaagcag tgggatggc gactcttat gataaaata 
      ataacgctt caaggacat gcgggtgaa gcgtgggct 
      ttggccgtg agtccaaac ggccagttt gtggtttcg 
      tgtggtgca gaccgcgtt ttgcgactg tacgaaaag 
      tcggaacag cctttggtg ctccaagac gaacaggag 
      gaagaaaga gagcaacag gaagtgctc gcaacgggg 
      gaacaaacg gttattccg ggtcagagt ggcttgaac 
      ttaccttcg aagaaaact gtggggagc gaaaaggcc 
      gctgagagc attctggag tgtttggaa atttgcgaa 
      aaatacaag gagcagtta gcggagcac gaggccctc 
      caggcggcc tcgagccgg acgctggcg ccgccgcag 
      ccccctttg ctcatgcaa gccctgggt gtgtcaacc 
      cctgacgac ttcctcttg gagacaatc aggaggatc 
      cgccccagt gacttggag gaggcgctt ctgctgctg 
      ccgtttagt tcagtttgc gaggtgttg caaattctg 
      ccggccttg atcgccaga ggcgaccag gtcgaattg 
      gtcgggaaa atttgcata ttcttattg aagttacac 
      cacgcaccc attgtggcc aaccacacg ctgctcgta 
      acgcttaaa caattgcaa aaattggcg ttcgccaaa 
      gtgcaggag ctgagggac cttgttggc tacaatttg 
      tacggtatg cagttcatt cagagggag attgaggcg 
      gctgaaggg gttcagttg ttccgcgac gccactttg 
      gagaagagg aagggcgac aagaagcgc aaacaacgc 
      gagaagttg aaacggtca atgattgtc ttgaatagc 
      tag
    mRNA sequence
    • >TC003001 RA
      ccacaactc aaattttga cataaatat aattaacca 
      atttttgtg tggttatgt ttaacttaa cctaacttt 
      ttacaaacg gcatgtgaa aaaaaattc agttccaca 
      cagttacaa gatgggttt aacaaagca atacctaag 
      atacgtccc ggccggcaa ctttaatat aatcgcgag 
      ccaaaactg caatctcaa atttgtcac acttgaagg 
      acaagaagg aagatttgt ggcggtcgg tgcatgcga 
      acatgtgat aatttggga cttgcgact cggggagaa 
      agctcaggt gctcccagg agaaaaggc cacagtgac 
      agccatctg tgccagccc caataaaag acacctagc 
      ggtcggtta ctccgatgg caacgtcca agtctacga 
      tttgaagtc ggccgaagt gattagcat tttctcagg 
      ccatcggtc agaagtcac aactttggc tttcgatag 
      ttacggaca caaattggc ctcaggatc taaagacac 
      ggacgttat cgtgtggga tacggtggc cgaaactgg 
      gctttgtag actctcagg tcacaaggg cccaattac 
      ccaaatcgc attcatgtc ccaacacaa tatcttgat 
      cagtggctc caaagacag cttcgtcaa attctggga 
      tttggacac ccaacactg tttcaagac actcacagg 
      ccacaggac tgaagtctg gggcttgag tttggttaa 
      agacgacca gtatttggt aacaggttg tggggacgc 
      agaattgag ggtttggaa gttatcaca aaaagaccc 
      cgattctga aaacaagag ctcaattga ccatttgac 
      atcaacgtt ggaactcat tgatttaca tgaatcaga 
      cgacttaaa tcacccttt acaatgtca caaaatagg 
      gtccttgtt gagagcggg caaaggccg agtggtctc 
      aatgttctg tgacggcag cggtcaagt gattggctg 
      ccacggcac tgatacaca actggaatt attttattt 
      ttgcacaga cgatgaggc acaagtgcg tttaaaaaa 
      acgactcaa gaaggagag aaagaagaa caaagaatt 
      ggaatctaa cgaccaaga ccaagagac gcaaagcca 
      ggacttgca gctgacttt aagggatca agtgaaacg 
      attggttcc aatcaaatt accaaataa acccaagtc 
      gttggattt ggtgcttgg aaatggggg cgaattgcg 
      agtctctgt taatttatc caacaacac cgttgaatt 
      gtacacaat tcagacggc tgtgaaaaa ctgtgaagc 
      cacgtgttt gagatctat cctcaatca gggacatca 
      cagtgaagt tagatctgt ttgtttcag taatgataa 
      tttagcgat tgcgtccgg aagtgctga ggccatcaa 
      gatgtggaa tcggccttc gcaagcctg tctcaggac 
      ggtggaaac agggtacat tttatcttg catctttgt 
      ccccggcga tcgtcacgt cttggcagg tctgaagaa 
      tggcacttt gctcatcat cgacattgc ggcggggga 
      tattttgga ggagattcc cgcacacag cgacgaact 
      ctgggccat ttgtttaca accagacca aaggggttg 
      tgtgactgg agggggcga caagaccgt caaattctg 
      gcaattcga gttagtggt cgacgagaa aagcgaaag 
      caaagccaa agtgctttc actcctcca cgtcaagac 
      gctgaagct cgaggactc agttctatg cgtcaaaat 
      ctcgcccaa tggcaaatt catcgcagt ggggctttt 
      ggacacgac agtcaagat atttttcgt cgatacttt 
      aaagtttta tctctcgtt atacggcca caaactgcc 
      agtcctttg cttggacat ttcgtccga ttctgcact 
      gattgccac agggtcagc tgaccgcaa tgtaaaaat 
      ctggggaat ggacttcgg tgactgcca caagagtat 
      ttttgccca cgacgattc ggtcactgg gctacagtt 
      cgttccgcg gacccatta cttcttcac ctgcgggaa 
      agacgggaa ggtcaagca gtgggatgg cgactctta 
      tgataaaat aataacgct tcaaggaca tgcgggtga 
      agcgtgggc tttggccgt gagtccaaa cggccagtt 
      tgtggtttc gtgtggtgc agaccgcgt tttgcgact 
      gtacgaaaa gtcggaaca gcctttggt gctccaaga 
      cgaacagga ggaagaaag agagcaaca ggaagtgct 
      cgcaacggg ggaacaaac ggttattcc gggtcagag 
      tggcttgaa cttaccttc gaagaaaac tgtggggag 
      cgaaaaggc cgctgagag cattctgga gtgtttgga 
      aatttgcga aaaatacaa ggagcagtt agcggagca 
      cgaggccct ccaggcggc ctcgagccg gacgctggc 
      gccgccgca gcccccttt gctcatgca agccctggg 
      tgtgtcaac ccctgacga cttcctctt ggagacaat 
      caggaggat ccgccccag tgacttgga ggaggcgct 
      tctgctgct gccgtttag ttcagtttg cgaggtgtt 
      gcaaattct gccggcctt gatcgccag aggcgacca 
      ggtcgaatt ggtcgggaa aatttgcat attcttatt 
      gaagttaca ccacgcacc cattgtggc caaccacac 
      gctgctcgt aacgcttaa acaattgca aaaattggc 
      gttcgccaa agtgcagga gctgaggga ccttgttgg 
      ctacaattt gtacggtat gcagttcat tcagaggga 
      gattgaggc ggctgaagg ggttcagtt gttccgcga 
      cgccacttt ggagaagag gaagggcga caagaagcg 
      caaacaacg cgagaagtt gaaacggtc aatgattgt 
      cttgaatag ctagtgaat tggattgaa agggttgtt 
      agcataata ttttcttat tattgtttt taagttcat 
      tacatatta tgtgaaaat aaacaaatc agtattct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY