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Gene information
General information
TC003022 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4299691..4301144
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003022
    iBeetle screen iB_07957

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003022 RA
      MCALVTFTS FALAATSAV LLVLAATNG KLKNNKMNN 
      IENNHTSLL DPDGPNVQF ILFTRSHPP FNLRINDFN 
      GFKKSGFNF SNPVKIIIH GFQSSIEED IFVVNKNAY 
      LDSGDYNVI GMDWSVLCE FEYLSAIGG VRKAGKVLG 
      EFLTWLSVL GVDYNNIHL VGHSLGAHV AGIGGHEVK 
      NGKIGRITG LDPAAPGFK DIEAKLKLD ANDAKMVDV 
      VHTYMKVLS LAQPVGHVD FYPNGGRRQ PGCPEISDI 
      WKFSESVIC NHARAYYYF AESIRNKRA FRSNRCNNV 
      EEALRMRCV QATSVYMGQ VESYKNGLY FFRTNAKRP 
      FSLSR
    CDS sequence
    • >TC003022 RA
      atgtgtgcg cttgttacc ttcacaagc tttgctcta 
      gctgcgact tcggccgtt ttgctcgtc ttggcggca 
      accaacgga aaactgaag aacaacaaa atgaacaat 
      atcgagaat aatcacacc agtctgctt gaccccgat 
      gggcccaac gtacagttt attttattc acaaggagc 
      catccccca tttaatctc agaattaac gatttcaac 
      gggttcaaa aaatcaggg ttcaatttc agcaatccg 
      gtcaagata attattcac ggctttcaa agcagcatt 
      gaggaggac atttttgtg gtcaacaaa aacgcttat 
      ttagattcg ggtgattac aatgttatt gggatggat 
      tggtccgtt ctgtgcgaa ttcgagtat ttatcggca 
      attggcgga gtgcgaaaa gccggcaaa gttctgggc 
      gaatttctc acttggttg tctgtactc ggggtcgat 
      tacaacaac atacacctg gtggggcac agtctgggg 
      gcgcacgtg gcggggatc gggggccac gaagtcaaa 
      aacgggaaa atcgggcgc attacaggc cttgatcct 
      gcagcccct ggcttcaag gacatcgag gccaagctg 
      aaactggac gcaaatgac gcgaaaatg gtcgatgtc 
      gtgcacacg tacatgaaa gtgttaagt cttgcacaa 
      cctgttggt cacgttgat ttttacccc aacgggggg 
      agacggcaa cccggttgt cccgaaata tccgacatc 
      tggaagttt agtgaaagt gtaatttgt aaccacgct 
      cgagcttat tattatttt gctgaatca atacggaac 
      aaaagggct ttcaggagc aacagatgt aacaatgtg 
      gaggaagcg cttcgtatg cggtgtgtt caagcgaca 
      agtgtttac atgggacaa gtcgagtct tataaaaat 
      gggttgtat tttttcagg accaatgcg aaaagaccg 
      ttcagtctc agtcgctga
    mRNA sequence
    • >TC003022 RA
      gatgcatga aatatggac cctccatta ttttgattg 
      tccatttcc atccaatgt gtggtgaaa gtgacaaaa 
      cattttttt ccactaaat gtgtgcgct tgttacctt 
      cacaagctt tgctctagc tgcgacttc ggccgtttt 
      gctcgtctt ggcggcaac caacggaaa actgaagaa 
      caacaaaat gaacaatat cgagaataa tcacaccag 
      tctgcttga ccccgatgg gcccaacgt acagtttat 
      tttattcac aaggagcca tcccccatt taatctcag 
      aattaacga tttcaacgg gttcaaaaa atcagggtt 
      caatttcag caatccggt caagataat tattcacgg 
      ctttcaaag cagcattga ggaggacat ttttgtggt 
      caacaaaaa cgcttattt agattcggg tgattacaa 
      tgttattgg gatggattg gtccgttct gtgcgaatt 
      cgagtattt atcggcaat tggcggagt gcgaaaagc 
      cggcaaagt tctgggcga atttctcac ttggttgtc 
      tgtactcgg ggtcgatta caacaacat acacctggt 
      ggggcacag tctgggggc gcacgtggc ggggatcgg 
      gggccacga agtcaaaaa cgggaaaat cgggcgcat 
      tacaggcct tgatcctgc agcccctgg cttcaagga 
      catcgaggc caagctgaa actggacgc aaatgacgc 
      gaaaatggt cgatgtcgt gcacacgta catgaaagt 
      gttaagtct tgcacaacc tgttggtca cgttgattt 
      ttaccccaa cggggggag acggcaacc cggttgtcc 
      cgaaatatc cgacatctg gaagtttag tgaaagtgt 
      aatttgtaa ccacgctcg agcttatta ttattttgc 
      tgaatcaat acggaacaa aagggcttt caggagcaa 
      cagatgtaa caatgtgga ggaagcgct tcgtatgcg 
      gtgtgttca agcgacaag tgtttacat gggacaagt 
      cgagtctta taaaaatgg gttgtattt tttcaggac 
      caatgcgaa aagaccgtt cagtctcag tcgctgaat 
      tattaatat actgcacca cggttgcaa tggggcact 
      tttttactg ttgtgttgt gtcaataaa ttataaaaa 
      acata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY