| General information | |||
| TC003390 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 4034546..4044090 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003390 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_07953 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
low validity due to technical reasons - should be repeated: cuticle phenotype not scorable,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC003390 RA
MFAQGFTSV LRSFLSSEP APTKVIDAK SDAFSDRAI HLREDGLVL YLPANTVDK KKGATYEIV LHRPYTESL HQTYSLYRS PNLDEAEVK FCVLKDKLP TFVEISKEM YNVQGLQKI CDVLAEHPS WNLAHLAAY FSLYDSFKD SKINNYLNS TDDETGISP LQVAITTKN LKTVQVLVA ANASLEHLD YNSDSVFHY AASTTKDII AALTQGSPP NCLNARNKN GYTPLHLAC QADKPDCVR ALLLAGADV NKTSGLIDD NVLPGYVGD YLQSHPNSL HKEDMKMGG TPLHWACSR EVIEALVDM NCHIDSLNF DKKTALHIM VMRNRLECV VALLSRQAD ANIGDAEGN RPIHLAVKQ GNISIIQCL IIFGVDLNI LNNAGHSPR HVITKEQEP KILYYLHAV GAQRCPPGT PYCTDGCVH GGTFDGVPP PPVIGPTNR DVLNQMLDV AAMEIASKK HKDGKMPKN GRLLCMDGG GIRGLVLVE MLLEIENVL QKPVSHCFD WIAGTSTGG ILALGIASG KTMKECLCL YFRIKELTF VGIRPYASE PLENVLKET FGAETVMAD ITHPKVMVT GVLADRKPV ELHLFRNYQ SPSDILQVE HNSPYELPP PPEEQYVWQ VGRATGAAP TYFRAFGYF LDGGLIANN PTLDALSEI HEHHLALKA IGREEDAAP VSVVVSLGT GVIPVTPVK DIDVFLPGS IFDGVKLYT GISTLGTLL VDQATASDG RVVDRARAW CSTIGVPYF RFSPQMSEE IAMNETSDE KLCKMLWET KAYMYKNLN TIKQIADLL NTS
- >TC003390 RA
atgtttgcc caaggattt acaagtgtt ttacgtagc ttcctcagt tcagaaccc gccccaacc aaagtgatt gacgccaaa tcagacgct ttttccgac cgagcgatt cacctacgg gaagatgga ttggtcctc tacctccca gctaataca gttgacaag aaaaaaggg gccacttac gaaattgtg ctccatcgc ccgtacacg gaaagccta caccaaacc tacagttta taccgctcg ccaaatctt gacgaagct gaagtgaaa ttttgtgtc ttgaaggat aagttaccc acgtttgtt gagatttcg aaagaaatg tataatgtg caaggtttg caaaagatt tgtgacgtt cttgcggaa cacccctct tggaacttg gcccatttg gcggcgtat tttagtctc tatgattct tttaaagac tctaagatt aacaattat ttgaatagt actgatgat gaaactggg atctcgcca ctacaagtt gctattacc acgaagaat ttgaagacg gtgcaagtt ttggttgct gcgaatgcg tctttggaa catctcgat tacaactct gattcggtg ttccattac gccgctagc accaccaaa gatatcatt gccgcgttg acgcaaggc agcccccca aactgctta aacgcccgc aacaaaaac ggctacaca ccactgcac cttgcctgc caagcggac aaacccgac tgcgtccga gccctcctt ctggccgga gccgacgta aacaaaacc tcaggtcta atagacgac aacgtccta cccggctac gttggcgac tacctccaa agccacccc aacagcctc cacaaggag gacatgaaa atggggggc accccttta cactgggcc tgttcccgc gaagtaatc gaagctcta gtcgacatg aactgccac atagactct ctaaacttc gacaaaaaa accgctttg cacatcatg gtcatgaga aatcgcctc gaatgcgtt gtagcttta ctgagtcgg caagcagac gcaaatatc ggcgatgcc gaaggcaac cgaccgatt catttagct gtgaaacaa ggcaacata tcgataatc cagtgcttg attattttc ggcgtcgat ttgaatatt ttgaacaat gccgggcat tcgcccaga catgttata acaaaggag caagagccg aaaatcttg tattacttg cacgccgtg ggggcgcag cggtgcccc cctggcacg ccctactgc accgacggg tgcgtgcat gggggcacc ttcgacggg gttccaccg cccccggtg atcggcccc actaaccgt gacgttctg aaccaaatg ctcgatgta gcagcgatg gaaatcgcc tcaaaaaaa cacaaagat gggaaaatg cccaaaaat gggcggctc ctgtgcatg gacgggggc ggaattcgg ggcctagtt ctggtggaa atgctgcta gaaattgaa aatgtttta cagaaacct gtaagtcat tgtttcgat tggattgcg ggaactagc actgggggg attctggca ctggggatt gcgagtggt aaaaccatg aaggagtgt ctgtgtttg tattttcgg attaaggag ctgacgttt gtggggatc aggccgtat gctagtgag ccgctggag aatgttttg aaggaaacg ttcggggcc gagactgtg atggcggat attacgcat ccgaaggtg atggttacg ggggtgctg gctgataga aaaccggtg gagttgcac ttgtttagg aattatcaa agtccgagc gatattttg caagtggag cataattcg ccgtatgag ttgccgcca ccgcccgaa gaacagtat gtgtggcag gttgggagg gccactggg gctgctccc acttatttc agagcgttt ggctacttt ctggacgga ggcctgatt gcaaacaac cccacactg gacgccctg tcggaaatc cacgagcac cacttggct ttgaaggcc atcggtcgt gaggaagac gccgctccg gtttccgta gtcgtcagt ttgggcaca ggggtgatc cctgtaacc cccgtcaag gatatcgac gtgtttctt cccggaagc atcttcgac ggagtcaag ctttatacc ggtatctca actttggga acacttcta gtcgatcag gcaactgct agtgatgga cgagtcgtg gacagagct agagcctgg tgctctacc attggagtg ccatacttt cgattttcc ccacaaatg tcggaggaa atcgcaatg aacgaaact tcggacgag aagttgtgc aaaatgctg tgggagacg aaggcgtac atgtataaa aatctcaac actattaag cagattgcc gatctactc aataccagt taa
- >TC003390 RA
tcttaaata aaacacaat gatcggtag tcaatctca gtgagtgat tagtgttcg taaccgtat gaatagata ttaagtaat aaaatgttt gcccaagga tttacaagt gttttacgt agcttcctc agttcagaa cccgcccca accaaagtg attgacgcc aaatcagac gctttttcc gaccgagcg attcaccta cgggaagat ggattggtc ctctacctc ccagctaat acagttgac aagaaaaaa ggggccact tacgaaatt gtgctccat cgcccgtac acggaaagc ctacaccaa acctacagt ttataccgc tcgccaaat cttgacgaa gctgaagtg aaattttgt gtcttgaag gataagtta cccacgttt gttgagatt tcgaaagaa atgtataat gtgcaaggt ttgcaaaag atttgtgac gttcttgcg gaacacccc tcttggaac ttggcccat ttggcggcg tattttagt ctctatgat tcttttaaa gactctaag attaacaat tatttgaat agtactgat gatgaaact gggatctcg ccactacaa gttgctatt accacgaag aatttgaag acggtgcaa gttttggtt gctgcgaat gcgtctttg gaacatctc gattacaac tctgattcg gtgttccat tacgccgct agcaccacc aaagatatc attgccgcg ttgacgcaa ggcagcccc ccaaactgc ttaaacgcc cgcaacaaa aacggctac acaccactg caccttgcc tgccaagcg gacaaaccc gactgcgtc cgagccctc cttctggcc ggagccgac gtaaacaaa acctcaggt ctaatagac gacaacgtc ctacccggc tacgttggc gactacctc caaagccac cccaacagc ctccacaag gaggacatg aaaatgggg ggcacccct ttacactgg gcctgttcc cgcgaagta atcgaagct ctagtcgac atgaactgc cacatagac tctctaaac ttcgacaaa aaaaccgct ttgcacatc atggtcatg agaaatcgc ctcgaatgc gttgtagct ttactgagt cggcaagca gacgcaaat atcggcgat gccgaaggc aaccgaccg attcattta gctgtgaaa caaggcaac atatcgata atccagtgc ttgattatt ttcggcgtc gatttgaat attttgaac aatgccggg cattcgccc agacatgtt ataacaaag gagcaagag ccgaaaatc ttgtattac ttgcacgcc gtgggggcg cagcggtgc ccccctggc acgccctac tgcaccgac gggtgcgtg catgggggc accttcgac ggggttcca ccgcccccg gtgatcggc cccactaac cgtgacgtt ctgaaccaa atgctcgat gtagcagcg atggaaatc gcctcaaaa aaacacaaa gatgggaaa atgcccaaa aatgggcgg ctcctgtgc atggacggg ggcggaatt cggggccta gttctggtg gaaatgctg ctagaaatt gaaaatgtt ttacagaaa cctgtaagt cattgtttc gattggatt gcgggaact agcactggg gggattctg gcactgggg attgcgagt ggtaaaacc atgaaggag tgtctgtgt ttgtatttt cggattaag gagctgacg tttgtgggg atcaggccg tatgctagt gagccgctg gagaatgtt ttgaaggaa acgttcggg gccgagact gtgatggcg gatattacg catccgaag gtgatggtt acgggggtg ctggctgat agaaaaccg gtggagttg cacttgttt aggaattat caaagtccg agcgatatt ttgcaagtg gagcataat tcgccgtat gagttgccg ccaccgccc gaagaacag tatgtgtgg caggttggg agggccact ggggctgct cccacttat ttcagagcg tttggctac tttctggac ggaggcctg attgcaaac aaccccaca ctggacgcc ctgtcggaa atccacgag caccacttg gctttgaag gccatcggt cgtgaggaa gacgccgct ccggtttcc gtagtcgtc agtttgggc acaggggtg atccctgta acccccgtc aaggatatc gacgtgttt cttcccgga agcatcttc gacggagtc aagctttat accggtatc tcaactttg ggaacactt ctagtcgat caggcaact gctagtgat ggacgagtc gtggacaga gctagagcc tggtgctct accattgga gtgccatac tttcgattt tccccacaa atgtcggag gaaatcgca atgaacgaa acttcggac gagaagttg tgcaaaatg ctgtgggag acgaaggcg tacatgtat aaaaatctc aacactatt aagcagatt gccgatcta ctcaatacc agttaattt acgataacg ttcaaattt ttagtaaat tattacatt ttttacaca accttttta gcacaatat tctttccgt agtcaattt tatcagtgt tgttaaaat gttgatcta gtatgagta cagaagtta cggacattt tgcccagtc tacacatta aggtaggta cagtagcgt aaaatttgg gggttgaca gtaaaatga aattttatt gccaaatta ctacaaaat attagaaaa tgttttatt tgataaaag tgaaataat tgagtaact aagaaaata agagtttgt atattttat tataatttt gtacatttc gctatcaat tcaaaaaaa aaaactaaa gaaaaatta tttagtcat agtggaaat tttccttct ctgacgtac ttctgaagc gttctttac tcaatttca ttttcttga tcttgtgga aataaaaat taaaatttt taatttgat tgaaacaaa tatacgtat tagaaaact acttatgac ataaacaaa ataataaaa aaatgtgat ttgtgagtg gaagaaaat tatgtaaga aaaattcgc ttgttaaat caatttatt aaaatcatt tcattaaat gttgcagca ctgtaacat tctaataaa taaaataaa gtaataagt gcatcgtag attcgtcaa ttcttatcg tttatcaat ttatcattg tctaatgtg aaaaaatgt accatttca ccagttcat acgtacttg tataattag ttaatgttg ttactttac tcggtgatt caaaagacc aacacataa ttaccttcc ctaagaaaa aaatttttt gtagtaaaa atttgtgtt tttaatgat ctatctatg gaaagttgc ttcaaaaaa tcacacaaa gtatggtac agttctttt gaatcacgc tgtatgtgg tgtataata ttttaataa gttataaat gataatgta gttaatttg gacaatcat tgttatcct gattatgtg atttttttc atggtgata ataatttta gtgtacgat taattaatt aatgtgcat atgaatatg aatgttaat atgtgttta aaaaacgga cgatacttc cctgtaggt gtccacatt tactttttt tttaattga agagttgag atttatctt attttctgc tattgtaaa tcagcacca aaattagtt ttctagtat tacagacaa atctgaagt tactttgtt ttgattttg ggaacttta gaactattt ttttttggg aattatgtt ctgccgtac tgacacttt attgttatt tattattgc agaacaggt ctgtccaaa atttcaaaa ataatctag tctgaatct gatgaatat ttttgcaag tggaacgta tcctaatat ttttttatt taaagtatg taagcaatt acacaatgt acgtccgta ggtattgtt tttcaaaca tgacaaata attatttag tgcattaca tgattaatt aatcaataa gtgtgcaaa aatgtgtag ttcagttgt gtgcttaat ttataaaaa catagatct tgagcgata cttattcag ggaattgta gattttatc aaaatt
