Status
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Gene information
General information
TC003390 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4034546..4044090
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003390
    iBeetle screen iB_07953

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (13 dpi)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    low validity due to technical reasons - should be repeated: cuticle phenotype not scorable,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003390 RA
      MFAQGFTSV LRSFLSSEP APTKVIDAK SDAFSDRAI 
      HLREDGLVL YLPANTVDK KKGATYEIV LHRPYTESL 
      HQTYSLYRS PNLDEAEVK FCVLKDKLP TFVEISKEM 
      YNVQGLQKI CDVLAEHPS WNLAHLAAY FSLYDSFKD 
      SKINNYLNS TDDETGISP LQVAITTKN LKTVQVLVA 
      ANASLEHLD YNSDSVFHY AASTTKDII AALTQGSPP 
      NCLNARNKN GYTPLHLAC QADKPDCVR ALLLAGADV 
      NKTSGLIDD NVLPGYVGD YLQSHPNSL HKEDMKMGG 
      TPLHWACSR EVIEALVDM NCHIDSLNF DKKTALHIM 
      VMRNRLECV VALLSRQAD ANIGDAEGN RPIHLAVKQ 
      GNISIIQCL IIFGVDLNI LNNAGHSPR HVITKEQEP 
      KILYYLHAV GAQRCPPGT PYCTDGCVH GGTFDGVPP 
      PPVIGPTNR DVLNQMLDV AAMEIASKK HKDGKMPKN 
      GRLLCMDGG GIRGLVLVE MLLEIENVL QKPVSHCFD 
      WIAGTSTGG ILALGIASG KTMKECLCL YFRIKELTF 
      VGIRPYASE PLENVLKET FGAETVMAD ITHPKVMVT 
      GVLADRKPV ELHLFRNYQ SPSDILQVE HNSPYELPP 
      PPEEQYVWQ VGRATGAAP TYFRAFGYF LDGGLIANN 
      PTLDALSEI HEHHLALKA IGREEDAAP VSVVVSLGT 
      GVIPVTPVK DIDVFLPGS IFDGVKLYT GISTLGTLL 
      VDQATASDG RVVDRARAW CSTIGVPYF RFSPQMSEE 
      IAMNETSDE KLCKMLWET KAYMYKNLN TIKQIADLL 
      NTS
    CDS sequence
    • >TC003390 RA
      atgtttgcc caaggattt acaagtgtt ttacgtagc 
      ttcctcagt tcagaaccc gccccaacc aaagtgatt 
      gacgccaaa tcagacgct ttttccgac cgagcgatt 
      cacctacgg gaagatgga ttggtcctc tacctccca 
      gctaataca gttgacaag aaaaaaggg gccacttac 
      gaaattgtg ctccatcgc ccgtacacg gaaagccta 
      caccaaacc tacagttta taccgctcg ccaaatctt 
      gacgaagct gaagtgaaa ttttgtgtc ttgaaggat 
      aagttaccc acgtttgtt gagatttcg aaagaaatg 
      tataatgtg caaggtttg caaaagatt tgtgacgtt 
      cttgcggaa cacccctct tggaacttg gcccatttg 
      gcggcgtat tttagtctc tatgattct tttaaagac 
      tctaagatt aacaattat ttgaatagt actgatgat 
      gaaactggg atctcgcca ctacaagtt gctattacc 
      acgaagaat ttgaagacg gtgcaagtt ttggttgct 
      gcgaatgcg tctttggaa catctcgat tacaactct 
      gattcggtg ttccattac gccgctagc accaccaaa 
      gatatcatt gccgcgttg acgcaaggc agcccccca 
      aactgctta aacgcccgc aacaaaaac ggctacaca 
      ccactgcac cttgcctgc caagcggac aaacccgac 
      tgcgtccga gccctcctt ctggccgga gccgacgta 
      aacaaaacc tcaggtcta atagacgac aacgtccta 
      cccggctac gttggcgac tacctccaa agccacccc 
      aacagcctc cacaaggag gacatgaaa atggggggc 
      accccttta cactgggcc tgttcccgc gaagtaatc 
      gaagctcta gtcgacatg aactgccac atagactct 
      ctaaacttc gacaaaaaa accgctttg cacatcatg 
      gtcatgaga aatcgcctc gaatgcgtt gtagcttta 
      ctgagtcgg caagcagac gcaaatatc ggcgatgcc 
      gaaggcaac cgaccgatt catttagct gtgaaacaa 
      ggcaacata tcgataatc cagtgcttg attattttc 
      ggcgtcgat ttgaatatt ttgaacaat gccgggcat 
      tcgcccaga catgttata acaaaggag caagagccg 
      aaaatcttg tattacttg cacgccgtg ggggcgcag 
      cggtgcccc cctggcacg ccctactgc accgacggg 
      tgcgtgcat gggggcacc ttcgacggg gttccaccg 
      cccccggtg atcggcccc actaaccgt gacgttctg 
      aaccaaatg ctcgatgta gcagcgatg gaaatcgcc 
      tcaaaaaaa cacaaagat gggaaaatg cccaaaaat 
      gggcggctc ctgtgcatg gacgggggc ggaattcgg 
      ggcctagtt ctggtggaa atgctgcta gaaattgaa 
      aatgtttta cagaaacct gtaagtcat tgtttcgat 
      tggattgcg ggaactagc actgggggg attctggca 
      ctggggatt gcgagtggt aaaaccatg aaggagtgt 
      ctgtgtttg tattttcgg attaaggag ctgacgttt 
      gtggggatc aggccgtat gctagtgag ccgctggag 
      aatgttttg aaggaaacg ttcggggcc gagactgtg 
      atggcggat attacgcat ccgaaggtg atggttacg 
      ggggtgctg gctgataga aaaccggtg gagttgcac 
      ttgtttagg aattatcaa agtccgagc gatattttg 
      caagtggag cataattcg ccgtatgag ttgccgcca 
      ccgcccgaa gaacagtat gtgtggcag gttgggagg 
      gccactggg gctgctccc acttatttc agagcgttt 
      ggctacttt ctggacgga ggcctgatt gcaaacaac 
      cccacactg gacgccctg tcggaaatc cacgagcac 
      cacttggct ttgaaggcc atcggtcgt gaggaagac 
      gccgctccg gtttccgta gtcgtcagt ttgggcaca 
      ggggtgatc cctgtaacc cccgtcaag gatatcgac 
      gtgtttctt cccggaagc atcttcgac ggagtcaag 
      ctttatacc ggtatctca actttggga acacttcta 
      gtcgatcag gcaactgct agtgatgga cgagtcgtg 
      gacagagct agagcctgg tgctctacc attggagtg 
      ccatacttt cgattttcc ccacaaatg tcggaggaa 
      atcgcaatg aacgaaact tcggacgag aagttgtgc 
      aaaatgctg tgggagacg aaggcgtac atgtataaa 
      aatctcaac actattaag cagattgcc gatctactc 
      aataccagt taa
    mRNA sequence
    • >TC003390 RA
      tcttaaata aaacacaat gatcggtag tcaatctca 
      gtgagtgat tagtgttcg taaccgtat gaatagata 
      ttaagtaat aaaatgttt gcccaagga tttacaagt 
      gttttacgt agcttcctc agttcagaa cccgcccca 
      accaaagtg attgacgcc aaatcagac gctttttcc 
      gaccgagcg attcaccta cgggaagat ggattggtc 
      ctctacctc ccagctaat acagttgac aagaaaaaa 
      ggggccact tacgaaatt gtgctccat cgcccgtac 
      acggaaagc ctacaccaa acctacagt ttataccgc 
      tcgccaaat cttgacgaa gctgaagtg aaattttgt 
      gtcttgaag gataagtta cccacgttt gttgagatt 
      tcgaaagaa atgtataat gtgcaaggt ttgcaaaag 
      atttgtgac gttcttgcg gaacacccc tcttggaac 
      ttggcccat ttggcggcg tattttagt ctctatgat 
      tcttttaaa gactctaag attaacaat tatttgaat 
      agtactgat gatgaaact gggatctcg ccactacaa 
      gttgctatt accacgaag aatttgaag acggtgcaa 
      gttttggtt gctgcgaat gcgtctttg gaacatctc 
      gattacaac tctgattcg gtgttccat tacgccgct 
      agcaccacc aaagatatc attgccgcg ttgacgcaa 
      ggcagcccc ccaaactgc ttaaacgcc cgcaacaaa 
      aacggctac acaccactg caccttgcc tgccaagcg 
      gacaaaccc gactgcgtc cgagccctc cttctggcc 
      ggagccgac gtaaacaaa acctcaggt ctaatagac 
      gacaacgtc ctacccggc tacgttggc gactacctc 
      caaagccac cccaacagc ctccacaag gaggacatg 
      aaaatgggg ggcacccct ttacactgg gcctgttcc 
      cgcgaagta atcgaagct ctagtcgac atgaactgc 
      cacatagac tctctaaac ttcgacaaa aaaaccgct 
      ttgcacatc atggtcatg agaaatcgc ctcgaatgc 
      gttgtagct ttactgagt cggcaagca gacgcaaat 
      atcggcgat gccgaaggc aaccgaccg attcattta 
      gctgtgaaa caaggcaac atatcgata atccagtgc 
      ttgattatt ttcggcgtc gatttgaat attttgaac 
      aatgccggg cattcgccc agacatgtt ataacaaag 
      gagcaagag ccgaaaatc ttgtattac ttgcacgcc 
      gtgggggcg cagcggtgc ccccctggc acgccctac 
      tgcaccgac gggtgcgtg catgggggc accttcgac 
      ggggttcca ccgcccccg gtgatcggc cccactaac 
      cgtgacgtt ctgaaccaa atgctcgat gtagcagcg 
      atggaaatc gcctcaaaa aaacacaaa gatgggaaa 
      atgcccaaa aatgggcgg ctcctgtgc atggacggg 
      ggcggaatt cggggccta gttctggtg gaaatgctg 
      ctagaaatt gaaaatgtt ttacagaaa cctgtaagt 
      cattgtttc gattggatt gcgggaact agcactggg 
      gggattctg gcactgggg attgcgagt ggtaaaacc 
      atgaaggag tgtctgtgt ttgtatttt cggattaag 
      gagctgacg tttgtgggg atcaggccg tatgctagt 
      gagccgctg gagaatgtt ttgaaggaa acgttcggg 
      gccgagact gtgatggcg gatattacg catccgaag 
      gtgatggtt acgggggtg ctggctgat agaaaaccg 
      gtggagttg cacttgttt aggaattat caaagtccg 
      agcgatatt ttgcaagtg gagcataat tcgccgtat 
      gagttgccg ccaccgccc gaagaacag tatgtgtgg 
      caggttggg agggccact ggggctgct cccacttat 
      ttcagagcg tttggctac tttctggac ggaggcctg 
      attgcaaac aaccccaca ctggacgcc ctgtcggaa 
      atccacgag caccacttg gctttgaag gccatcggt 
      cgtgaggaa gacgccgct ccggtttcc gtagtcgtc 
      agtttgggc acaggggtg atccctgta acccccgtc 
      aaggatatc gacgtgttt cttcccgga agcatcttc 
      gacggagtc aagctttat accggtatc tcaactttg 
      ggaacactt ctagtcgat caggcaact gctagtgat 
      ggacgagtc gtggacaga gctagagcc tggtgctct 
      accattgga gtgccatac tttcgattt tccccacaa 
      atgtcggag gaaatcgca atgaacgaa acttcggac 
      gagaagttg tgcaaaatg ctgtgggag acgaaggcg 
      tacatgtat aaaaatctc aacactatt aagcagatt 
      gccgatcta ctcaatacc agttaattt acgataacg 
      ttcaaattt ttagtaaat tattacatt ttttacaca 
      accttttta gcacaatat tctttccgt agtcaattt 
      tatcagtgt tgttaaaat gttgatcta gtatgagta 
      cagaagtta cggacattt tgcccagtc tacacatta 
      aggtaggta cagtagcgt aaaatttgg gggttgaca 
      gtaaaatga aattttatt gccaaatta ctacaaaat 
      attagaaaa tgttttatt tgataaaag tgaaataat 
      tgagtaact aagaaaata agagtttgt atattttat 
      tataatttt gtacatttc gctatcaat tcaaaaaaa 
      aaaactaaa gaaaaatta tttagtcat agtggaaat 
      tttccttct ctgacgtac ttctgaagc gttctttac 
      tcaatttca ttttcttga tcttgtgga aataaaaat 
      taaaatttt taatttgat tgaaacaaa tatacgtat 
      tagaaaact acttatgac ataaacaaa ataataaaa 
      aaatgtgat ttgtgagtg gaagaaaat tatgtaaga 
      aaaattcgc ttgttaaat caatttatt aaaatcatt 
      tcattaaat gttgcagca ctgtaacat tctaataaa 
      taaaataaa gtaataagt gcatcgtag attcgtcaa 
      ttcttatcg tttatcaat ttatcattg tctaatgtg 
      aaaaaatgt accatttca ccagttcat acgtacttg 
      tataattag ttaatgttg ttactttac tcggtgatt 
      caaaagacc aacacataa ttaccttcc ctaagaaaa 
      aaatttttt gtagtaaaa atttgtgtt tttaatgat 
      ctatctatg gaaagttgc ttcaaaaaa tcacacaaa 
      gtatggtac agttctttt gaatcacgc tgtatgtgg 
      tgtataata ttttaataa gttataaat gataatgta 
      gttaatttg gacaatcat tgttatcct gattatgtg 
      atttttttc atggtgata ataatttta gtgtacgat 
      taattaatt aatgtgcat atgaatatg aatgttaat 
      atgtgttta aaaaacgga cgatacttc cctgtaggt 
      gtccacatt tactttttt tttaattga agagttgag 
      atttatctt attttctgc tattgtaaa tcagcacca 
      aaattagtt ttctagtat tacagacaa atctgaagt 
      tactttgtt ttgattttg ggaacttta gaactattt 
      ttttttggg aattatgtt ctgccgtac tgacacttt 
      attgttatt tattattgc agaacaggt ctgtccaaa 
      atttcaaaa ataatctag tctgaatct gatgaatat 
      ttttgcaag tggaacgta tcctaatat ttttttatt 
      taaagtatg taagcaatt acacaatgt acgtccgta 
      ggtattgtt tttcaaaca tgacaaata attatttag 
      tgcattaca tgattaatt aatcaataa gtgtgcaaa 
      aatgtgtag ttcagttgt gtgcttaat ttataaaaa 
      catagatct tgagcgata cttattcag ggaattgta 
      gattttatc aaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY